Methanol fraction of Calliandra portoricensis root bark activates caspases via alteration in mitochondrial viability in vivo

Introduction: Dysregulated apoptosis is associated with a number of disease conditions. Traditionally, Calliandra portoricensis is used in the management of prostate enlargement. This study investigates the in vivo effect of potent methanol fraction of C. portoricensis (MFCP) on mitochondrial permeability transition (mPT) pore, an important pharmacological target in treatment of various diseases, and examines the toxicities associated with its oral administration. Methods: Forty-two male Wistar strain rats (70-80 g) were divided into 6 groups of 7 animals each. Each group was orally administered 25, 50, 100, 200, 400 mg/kg MFCP and the control group received distilled water for 21 and 30 days, respectively. mPT, assay for serum enzymes and hematological parameters were assessed spectrophotometrically while activation of caspases 3 and 9 was done by ELISA technique. Histological assessment of vital organs (liver, kidney, prostate) was carried out according to standard procedures. Results: There were no significant effects on mPT pore at all doses administered after 21 days of oral administration. However, after 30 days of administration, MFCP induced mPT pore opening at doses 100 and 200 mg/kg with induction folds of 2.6 and 3.3, respectively while there was no induction of mPT pore opening at lower doses of 25 mg/kg and 50 mg/kg. Furthermore, significant (P < 0.05) increases in serum enzymes (ALT, AST) were observed at all doses administered when compared with control after 30 days of oral administration. Cell counts (Hb, PCV, RBC, WBC) were adversely affected at the highest dose (200 mg/kg) compared with control and other treated groups (25, 50 and 100 mg/kg) after 30 days of administration. Similarly, activation of caspases 9 and 3 were observed in rat liver homogenate at high doses of the fraction while histological evaluation showed degeneration and distortion of organs at the highest dose. Conclusion: MFCP contains phytochemicals that elicit the opening of the pore and induction of mitochondrial-mediated apoptosis. This would be relevant in treatment of degenerative diseases that results from down-regulation of apoptosis. However, caution should be exercised in using high doses of the plant

A Low-Cost Instrument for Estimating the Starch Content of Cassava Roots Based on the Measurement of RF Return Loss

The problem of simply and reliably estimating starch content of cassava roots in the field is addressed by the development of a low cost test instrument that measures return loss at radio frequencies using a coaxial probe. A clear relationship between starch content of cassava roots and the measured return loss of root samples at a specific frequency of 30 MHz is first verified experimentally. A prototype test instrument is then designed with goals of portability, low cost and simplicity of use. The test instrument displays starch content in 5 categories, from “low” to “high” using an array of 5 LEDs. The performance of the test instrument is experimentally verified in the field and a reliable correlation between cassava root starch content and LED indication is demonstrated

Extraction of a low grade zinc ore using gravity and froth flotation methods

Extraction of low grade zinc ore found in Gumau- Toro town was carried out using gravity and froth flotation methods. Analysis of the chemical composition reveals that the crude ore contains 20.20% Zinc and other related minerals such as  66.72% SiO2, 2.36%.Pb, 16.62%Fe, 0.001%Ag, these are impurities that  hindered the grade of zinc in this ore from appreciating. Fractional and grain sieve size results revealed that the liberation size of the ore is at -355+250μm assaying 32.41%Zn. At this liberation sieve size, the low grade zinc ore was subjected to four different beneficiation methods using five kilograms (5kg) each as charge into  Wilfley shaking Table, Humphrey spiral concentrator, Air floating Machine and one kilogram (1kg) for froth flotation. Resulting products (concentrate and tailing) from these processes were analyzed using instrumentation method. Percentage recovery of each applied process was calculated. This was used to ascertain that the use of froth flotation that gives 74.32%Zn at a recovery of 84.62% stands out the best method for zinc extraction towards value addition to the low grade crude zinc ore. ©JASEMKey words: Extraction, Upgrading, Gravity, Froth flotation, Mineral, Fractional  analysis.

A rapid review of the evidence for children’s TV and online advertisement restrictions to fight obesity

The World Health Organisation has urged all governments to address rising rates of obesity by implementing population-based interventions, such as restrictions on the marketing to children of unhealthy food and beverage items. However, the relationship between unhealthy food advertisements and childhood obesity is disputed by industry-sponsored reports, which recommend promoting physical activity and weight loss campaigns rather than policies to limit exposure to advertisements. We aimed to elucidate this debate by providing a narrative review of the evidence on the relationship between unhealthy TV and online food advertisements, short-term food consumption and childhood obesity. We also examined the impact of unhealthy food advertisements on vulnerable groups and identified which policy interventions are supported by current evidence. We conducted a rapid overview of reviews published since 2006. From a synthesis of 18 reviews meeting the inclusion criteria, we conclude that exposure to unhealthy TV and online food advertising is a contributing factor to childhood obesity. Evidence of a relationship between exposure to unhealthy food advertisements and childhood obesity was evident at all stages of the causal pathway, including a clear dose-response relationship. The evidence base was particularly strong for children aged 3–12 years of age and for children from socio-economically disadvantaged and minority ethnic backgrounds. The introduction of statutory regulation is a potentially cost-effective policy option, in terms of healthcare savings outweighing the costs of implementing the policy, although voluntary codes were shown to be ineffective, with exposure to unhealthy food advertisements similar in countries before and after their introduction. Food advertising, however, is just one factor in the wider obesogenic environment and further advertising restrictions must be implemented alongside population-based interventions that aim to address systemic causes of poor diet

COVID-19 Outcomes in Minority Ethnic Groups: Do Obesity and Metabolic Risk Play a Role?

Purpose of Review Globally, minority ethnic groups have been at higher risk of COVID-19 mortality and morbidity than majority populations. This review outlines factors that may interact to create these inequalities and explores the hypothesis that differing levels of cardio-metabolic risk, according to ethnic group, play a role. Recent Findings Two UK Biobank studies have reported that the body mass index is more strongly associated with an increased risk of COVID-19 infection and mortality in minority ethnic populations than in White populations. A study of UK patients found that the strongest association between obesity and adverse COVID-19 outcomes was in people of Black ethnicity. Summary Differences in the prevalence of obesity and its metabolic sequelae have been shown to partly mediate ethnic inequalities in COVID-19 outcomes, although not always consistently. It is possible that ethnic differences in the consequences of obesity may explain some of the remaining disparity in COVID-19 risk

Succinylation of potato (Ipomoea batatas) starch: effect of varying ethanol concentration on pasting, structural, morphological, and functional properties

The shortcomings arising from usage of native starches in various industrial processes have demanded the need for modification in order to enhance its functionality. This study investigated the impact of ethanol concentration on pasting, structural, morphological, and functional properties of succinylated potato starch. Some granules of the potato starch sample also appeared to be multilobed and fractured. The Fourier transform -1 infrared spectroscopy (FT-IR) study revealed the presence of carbonyl group (1747 cm-1 ) in the starch chains as an additional functional group after succinylation. The statistical analysis of the study data revealed a significant (p &lt; 0.05) decrease in protein, fat, fibre, ash, and reduction in moisture contents after modification. Analysis with visco-analyzer showed reduction in pasting parameters after modification. Modification of native potato starch with ethanol improved the functionality of the starch by imparting some additional physicochemical and functional properties. These improved properties upon modification, therefore, suggest some potential favorable qualities for special applications

Improving the health and welfare of people who live in slums

Summary In the first paper in this Series we assessed theoretical and empirical evidence and concluded that the health of people living in slums is a function not only of poverty but of intimately shared physical and social environments. In this paper we extend the theory of so-called neighbourhood effects. Slums offer high returns on investment because beneficial effects are shared across many people in densely populated neighbourhoods. Neighbourhood effects also help explain how and why the benefits of interventions vary between slum and non-slum spaces and between slums. We build on this spatial concept of slums to argue that, in all low-income and-middle-income countries, census tracts should henceforth be designated slum or non-slum both to inform local policy and as the basis for research surveys that build on censuses. We argue that slum health should be promoted as a topic of enquiry alongside poverty and health

Psychosocial interventions for people with dementia: An overview and commentary on recent developments

YesAn influential review in 2010 concluded that non-pharmacological multi-component interventions have positive effects on cognitive functioning, activities of daily living, behaviour and mood of people with dementia. Our aim here is to provide an up-to-date overview of research into psychosocial interventions and their impact on psychosocial outcomes. We focused on randomised controlled trials, controlled studies and reviews published between October 2008 and August 2015, since the earlier review. The search of PsychInfo, Medline and the Cochrane database of systematic reviews yielded 61 relevant articles, organised into four themes echoing key phases of the care pathway: Living at home with dementia (five reviews, eight studies), carer interventions (three reviews, four studies), interventions in residential care (16 reviews, 12 studies) and end-of-life care (three reviews, two studies), along with an additional group spanning community and institutional settings (six reviews, two studies). Community findings suggested that appointment of dementia specialists and attention to case management can produce positive outcomes; physical therapies, cognitive training and modified cognitive behaviour therapy also had a range of benefits. There was more limited evidence of positive benefits for people with dementia through interventions with family carers. Thirty-two articles focused on the management of ‘behavioural symptoms’ through a range of interventions all of which had some evidence of benefit. Also a range of multi-component and specific interventions had benefits for cognitive, emotional and behavioural well-being of people with dementia in residential settings, as well as for quality of life. Overall, interventions tended to be short term with impact only measured in the short term. We recommend further research on interventions to promote living well in the community post-diagnosis and to address end-of-life care. Development of psychosocial interventions would benefit from moving beyond the focus on control of behaviours to focus on wider aspects of life for people with dementia

Ethical implications of the perception and portrayal of dementia

yesThe way we perceive and portray dementia has implications for how we act towards people with dementia and how we address the issue of dementia within society. As a multi-disciplinary working group, established within the framework of the European Dementia Ethics Network of Alzheimer Europe, we aimed to describe the different ways that people with dementia are perceived and portrayed within society and to consider the moral implications of this. In the current paper, we address perceptions of dementia as reflected in explanatory models of its cause and nature, descriptions of characteristics of people with dementia, the use of language, media portrayals and the views of people living with dementia. Academics and professionals could use this exploration to reflect on their behaviour and their use of language regarding people with dementiaThe taskforce’s work arises from the 2013 Work Plan of Alzheimer Europe, which received funding from the European Union in the framework of the Health Programme

Text-message reminders increase uptake of routine breast screening appointments : a randomised controlled trial in a hard-to-reach population

Background: There is a need for interventions to promote uptake of breast screening throughout Europe. Methods: We performed a single-blind randomised controlled trial to test whether text-message reminders were effective. Two thousand two hundred and forty women receiving their first breast screening invitation were included in the study and randomly assigned in a 1 : 1 ratio to receive either a normal invitation only (n=1118) or a normal invitation plus a text-message reminder 48 h before their appointment (n=1122). Findings: In the intention-to-treat analysis, uptake of breast screening was 59.1% among women in the normal invitation group and 64.4% in the text-message reminder group (χ2=6.47, odds ratio (OR): 1.26, 95% confidence intervals (CI): 1.05–1.48, P=0.01). Of the 1122 women assigned to the text-message reminder group, only 456 (41%) had a mobile number recorded by their GP and were thereby sent a text. In the per-protocol analysis, uptake by those in the control group who had a mobile number recorded on the GP system was 59.77% and by those in the intervention group who were sent a reminder 71.7% (χ2=14.12, OR=1.71, 95% CI=1.29–2.26, P<0.01). Interpretation: Sending women a text-message reminder before their first routine breast screening appointment significantly increased attendance. This information can be used to allocate resources efficiently to improve uptake without exacerbating social inequalities