The Gilboa Dam and its possible failure : what it means for NYC and Upstate New York

The majority of the NYC water supply is provided by a system of dams and reservoirs that have been created in upstate New York. As the owner of this water supply system, NYC is responsible for managing each of the dams and reservoirs within the system. This responsibility includes upholding dam regulations in order to ensure the safety of the citizens of the surrounding area and to lesson any environmental impacts that may occur due to the water supply system. Of the 30 dams owned by NYC, however, 25 of them have been identified at a high-hazard level according the National Inventory of Dams. The Gilboa Dam, which is located in Schoharie County along the Schoharie Creek, is part of the Catskill water supply system of NYC and is one of the NYC dams that has recently been classified at a high-hazard level. The possibility of the Gilboa Dam failing is just one of many problems NYC has had to mitigate with regard to its water supply system. While this thesis will focus primarily on the Gilboa Dam crisis, it will also examine the management of dams at a national level in order to recommend future policies in dam projects

A device-dependent interface for interactive image display

The structure of the device independent Display Management Subsystem (DMS) and the interface routines that are available to the applications programmer for use in developing a set of portable image display utility programs are described

Rural Kansas Family Physician Satisfaction with Caring for Spanish-Speaking Only Patients

Introduction. Patient satisfaction with the care they receive can beinfluenced negatively by a language barrier between the physician andpatient. However, there is a paucity of information regarding the consequencesof a language barrier on physician satisfaction, althoughthis barrier has the potential to decrease physician wellness. Thisstudy sought to determine if a language barrier is a source of professionaldissatisfaction in family medicine physicians in rural Kansas. Methods. In a cross-sectional study, members of the Kansas Academyof Family Physicians who practiced in the rural Kansas countieswith the highest percentage of Hispanic residents were surveyed. Aquestionnaire was developed to determine the demographics of thephysician, details regarding his or her practice, and percentage of Hispanicand Spanish-speaking only (SSO) patients in their practice.Physicians also were queried as to their level of Spanish-speakingability, availability of certified interpreters, and their satisfaction withcaring for their SSO patients. Results. Fifty-two physicians were identified and sent questionnairesby mail. Eighteen questionnaires were completed and returned, resultingin a 34% response rate. Respondents remained anonymous. In thepractices surveyed, 61% of practice settings had a Hispanic-patientpopulation greater than 25%. Only one of the eighteen respondentshad greater than 25% of SSO patients in his or her practice. A certifiedinterpreter was used less than 25% of the time in over 75% ofthe clinical encounters with SSO patients. Seventy-five percent ofphysicians reported no difficulty establishing trust and rapport withtheir SSO patients. Eighty-nine percent of respondents rated theirrelationship with SSO patients as good to excellent, and 83% weresatisfied with the care they were able to provide this group. Seventyeightpercent of respondents reported that their ability to care forSSO patients decreased or had no effect on their professional satisfaction.Seventy-eight percent of physicians also rated their overallprofessional satisfaction in regards to their physician/patient relationshipas good to excellent. However, language barriers affectedphysician-patient relationships, physician satisfaction with care, andprofessional satisfaction. Conclusion. Language barrier affected physician’s relationships withSSO patients, led to decreased physician satisfaction with the carethey provided and to decreased professional satisfaction.KS J Med 2017;10(4):79-83

On-Orbit Evaluation of a New Treadmill Harness for Improved Crewmember Comfort and Load Distribution

The current design of the International Space Station (ISS) Treadmill Harness has been reported to cause pain and discomfort to crewmembers during exercise. The Harness Station Development Test Objective (SDTO) provided participating crewmembers (n = 6) with a new harness design, the "Glenn Harness," to evaluate for comfort and loading as compared to the current Treadmill Harness. A novel suite of load-sensing instrumentation was developed to noninvasively measure load distribution and provided a first-ever quantification of actual dynamic loads during treadmill exercise. In addition, crew debriefs provided feedback on harness preference and overall impressions. Conclusions: Post-flight analysis in returned Glenn Harnesses (n = 3) showed minimal wear and tear. Four of the six subjects found the Glenn Harness to be more comfortable in this on-orbit, side-by-side comparison as measured by the crew comfort questionnaire and crew debriefs. Specific areas for improvement have been identified, and forward recommendations will be provided to the Human Research Program. The protocol developed for the SDTO provided valuable insight into crew comfort issues, design improvements, and loading preferences for exercise harnessing, which lays the groundwork for better harnessing systems and training protocols

Population Structure Analyses Provide Insight into the Source Populations Underlying Rural Isolated Communities in Illinois

We have previously hypothesized that relatively small and isolated rural communities may experience founder effects, defined as the genetic ramifications of small population sizes at the time of a community’s establishment. To explore this, we used an Illumina Infinium Omni2.5Exome-8 chip to collect data from 157 individuals from four Illinois communities, three rural and one urban. Genetic diversity estimates of 999,259 autosomal markers suggested that the reduction in heterozygosity due to shared ancestry was approximately 0, indicating a randomly mating population. An eigenanalysis, which is similar to a principal component analysis but ran on a genetic coancestry matrix, conducted in the SNPRelate R package revealed that the majority of these individuals formed one cluster with a few putative outliers obscuring population variation. An additional eigenanalysis on the same markers in a combined data set including the 2,504 individuals in the 1000 Genomes database found that most of the 157 Illinois individuals clustered into one group in close proximity to individuals of European descent. A final eigenanalysis of the Illinois individuals with the 503 individuals of European descent (within the 1000 Genomes Project) revealed two clusters of individuals and likely two source populations; one British and one consisting of multiple European subpopulations. We therefore demonstrate the feasibility of examining genetic relatedness across Illinois populations and assessing the number of source populations using publicly available databases. When assessed, it becomes possible for population structure information to contribute to the understanding of genetic history in rural populations

Genetic structure of First Nation communities in the Pacific Northwest

This study presents genetic data for nine Native American populations from northern North America. Analyses of genetic variation focus on the Pacific Northwest (PNW). Using mitochondrial, Y chromosomal and autosomal DNA variants, we aim to more closely address the relationships of geography and language with present genetic diversity among the regional PNW Native American populations. Patterns of genetic diversity exhibited by the three genetic systems were consistent with our hypotheses, in that we expected genetic variation to be more strongly explained by geographic proximity than linguistic structure. Our findings were corroborated through a variety on analytic approaches, with the unrooted trees for the three genetic systems consistently separating inland from coastal PNW populations. Furthermore, the AMOVA tests support the trends exhibited by the unrooted trees, with geographic partitioning of PNW populations (FCT = 19.43%, p = 0.010 ± 0.009) accounting for over twice as much of the observed genetic variation compared with linguistic partitioning of the same populations (FCT = 9.15%, p = 0.193 ± 0.013). These findings demonstrate a consensus with previous PNW population studies examining the relationships of genome-wide variation, mitochondrial haplogroup frequencies, and skeletal morphology with geography and language

From cheek swabs to consensus sequences : an A to Z protocol for high-throughput DNA sequencing of complete human mitochondrial genomes

Background: Next-generation DNA sequencing (NGS) technologies have made huge impacts in many fields of biological research, but especially in evolutionary biology. One area where NGS has shown potential is for high-throughput sequencing of complete mtDNA genomes (of humans and other animals). Despite the increasing use of NGS technologies and a better appreciation of their importance in answering biological questions, there remain significant obstacles to the successful implementation of NGS-based projects, especially for new users. Results: Here we present an ‘A to Z’ protocol for obtaining complete human mitochondrial (mtDNA) genomes – from DNA extraction to consensus sequence. Although designed for use on humans, this protocol could also be used to sequence small, organellar genomes from other species, and also nuclear loci. This protocol includes DNA extraction, PCR amplification, fragmentation of PCR products, barcoding of fragments, sequencing using the 454 GS FLX platform, and a complete bioinformatics pipeline (primer removal, reference-based mapping, output of coverage plots and SNP calling). Conclusions: All steps in this protocol are designed to be straightforward to implement, especially for researchers who are undertaking next-generation sequencing for the first time. The molecular steps are scalable to large numbers (hundreds) of individuals and all steps post-DNA extraction can be carried out in 96-well plate format. Also, the protocol has been assembled so that individual ‘modules’ can be swapped out to suit available resources

Ancient and modern genomics of the Ohlone Indigenous population of California

Traditional knowledge, along with archaeological and linguistic evidence, documents that California supports cultural and linguistically diverse Indigenous populations. Studies that have included ancient genomes in this region, however, have focused primarily on broad-scale migration history of the North American continent, with relatively little attention to local population dynamics. Here, in a partnership involving researchers and the Muwekma Ohlone tribe, we analyze genomic data from ancient and present-day individuals from the San Francisco Bay Area in California: 12 ancient individuals dated to 1905 to 1826 and 601 to 184 calibrated years before the present (cal BP) from two archaeological sites and eight present-day members of the Muwekma Ohlone tribe, whose ancestral lands include these two sites. We find that when compared to other ancient and modern individuals throughout the Americas, the 12 ancient individuals from the San Francisco Bay Area cluster with ancient individuals from Southern California. At a finer scale of analysis, we find that the 12 ancient individuals from the San Francisco Bay Area have distinct ancestry from the other groups and that this ancestry has a component of continuity over time with the eight present-day Muwekma Ohlone individuals. These results add to our understanding of Indigenous population history in the San Francisco Bay Area, in California, and in western North America more broadly

AACP Special Taskforce White Paper on Diversifying Our Investment in Human Capital

The 2015-2017 American Association of Colleges of Pharmacy (AACP) Special Taskforce on Diversifying our Investment in Human Capital was appointed for a two-year term, due to the rigors and complexities of its charges. This report serves as a white paper for academic pharmacy on diversifying our investment in human capital. The Taskforce developed and recommended a representation statement that was adapted and adopted by the AACP House of Delegates at the 2016 AACP Annual Meeting. In addition, the Taskforce developed a diversity statement for the Association that was adopted by the AACP Board of Directors in 2017. The Taskforce also provides recommendations to AACP and to academic pharmacy in this white paper