5 research outputs found

    Response to correspondence on Reproducibility of CRISPR-Cas9 Methods for Generation of Conditional Mouse Alleles: A Multi-Center Evaluation

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    Revue d'histoire du Bas Saint-Laurent, vol. 5 (2)

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    Éditorial: Entre deux tendances... -- Jules Brillant et l'universitĂ© rurale du Bas St-Laurent -- Les origines du collĂšge-sĂ©minaire de Rimouski -- Les ursulines de Rimouski -- À l'oeuvre dans l'Est du QuĂ©bec -- Mon Ă©cole d'il y a cinquante ans -- La gĂ©nĂ©alogie -- C.H.N.C., New Carlisle, la premiĂšre station de radio française dans l'Est du QuĂ©be

    Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation

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    BackgroundCRISPR-Cas9 gene-editing technology has facilitated the generation of knockout mice, providing an alternative to cumbersome and time-consuming traditional embryonic stem cell-based methods. An earlier study reported up to 16% efficiency in generating conditional knockout (cKO or floxed) alleles by microinjection of 2 single guide RNAs (sgRNA) and 2 single-stranded oligonucleotides as donors (referred herein as “two-donor floxing” method).ResultsWe re-evaluate the two-donor method from a consortium of 20 laboratories across the world. The dataset constitutes 56 genetic loci, 17,887 zygotes, and 1718 live-born mice, of which only 15 (0.87%) mice contain cKO alleles. We subject the dataset to statistical analyses and a machine learning algorithm, which reveals that none of the factors analyzed was predictive for the success of this method. We test some of the newer methods that use one-donor DNA on 18 loci for which the two-donor approach failed to produce cKO alleles. We find that the one-donor methods are 10- to 20-fold more efficient than the two-donor approach.ConclusionWe propose that the two-donor method lacks efficiency because it relies on two simultaneous recombination events in cis, an outcome that is dwarfed by pervasive accompanying undesired editing events. The methods that use one-donor DNA are fairly efficient as they rely on only one recombination event, and the probability of correct insertion of the donor cassette without unanticipated mutational events is much higher. Therefore, one-donor methods offer higher efficiencies for the routine generation of cKO animal models

    Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation

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    Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation

    No full text
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