Variation of bone mineral density induced by exercise in postmenopausal women

OBJETIVO: Evaluar la variación de la densidad mineral ósea (DMO) en columna lumbar (CL) y cuello femoral (CF).MÉTODO: 77 mujeres con osteopenia y menopausia, formaron cuatro grupos. Dos con ejercicio físico: uno tratamiento de estrógeno/Calcitonina (n=16), y otro ingesta de Calcio/Vitamina D (n=7). Dos sin ejercicio físico, uno tratamiento estrógeno/Calcitonina (n=27) y otro ingesta Calcio/Vitamina D (n=27). El ejercicio consistió en Fuerza Muscular (65% a 75% de 1RM) y multisaltos de baja a moderada intensidad. La DMO se midió mediante absorciometría. RESULTADOS: En ANOVA 2x2 se encontró homogeneidad (p<0,05) en la DMO tanto en CL y CF entre los tratamientos farmacológicos y nutricionales, heterogeneidad entre los grupos activos y sedentarios (CL p<0,05, CF p<0,01) y heterogeneidad en la interacción (p<0,01) de ambos tratamientos con el ejercicio físico. CONCLUSIONES: El ejercicio físico programado junto con los tratamientos habituales conduce a un mayor incremento de la DMO de en mujeres postmenopáusicasOBJECTIVE: Assess the variation of Bone Mineral Density (BMD) in Lumbar Spine (LS) and Femoral Neck (FN). METHOD: 77 postmenopausal women with osteopenia. Four groups, two groups with exercise: one Estrogen/Calcitonin treatment (n=16), another supplement Calcium/Vitamin D (n=7); two groups without exercise, one Estrogen/Calcitonin treatment (n=27), and another supplement Calcium/Vitamin D (n=27). The exercise program consisted of resistance training (65% to 75% 1RM) and multi-jumps of low-to-moderate intensity. The BMD was evaluated by absorptiometry. RESULTS. ANOVA 2x2, homogeneity (p<0,05) in the BMD in both LS and FN between the pharmacological and nutritional treatments, and heterogeneity between the active and sedentary groups (LS p <0,05, FN p<0,01) as also in the interaction (p<0,01) of the pharmacological/nutritional treatments with the physical exercise were found. CONCLUSION. Physical exercise program with the usual treatments, lead to a greater increase in the BMD of LS and FN in postmenopausal wome

Antimicrobial Peptides Pom-1 and Pom-2 from Pomacea poeyana Are Active against Candida auris, C. parapsilosis and C. albicans Biofilms

Recently two peptides isolated from the Cuban freshwater snail Pomacea poeyana (Pilsbry, 1927) were described to have antimicrobial activity against bacterial pathogens. Here we show considerable activities of Pom-1 and Pom-2 to reduce the viability of C. albicans, C. parapsilosis and the less common species C. auris measured as the decrease of metabolic activity in the resazurin reduction assay for planktonic cells. Although these activities were low, Pom-1 and Pom-2 turned out to be highly potent inhibitors of biofilm formation for the three Candida species tested. Whereas Pom-1 was slightly more active against C. albicans and C. parapsilosis as representatives of the more common Candida species Pom-2 showed no preference and was fully active also against biofilms of the more uncommon species C. auris. Pom-1 and Pom-2 may represent promising lead structures for the development of a classical peptide optimization strategy with the realistic aim to further increase antibiofilm properties and other pharmacologic parameters and to generate finally the first antifungal drug with a pronounced dedication against Candida biofilms

Tectonosedimentary evolution of the Coastal Cordillera and Central Depression of south-Central Chile (36°30′-42°S)

The forearc of south-central Chile (36°30′-42°S) is characterized by the presence of a Coastal Cordillera and a low lying area known as the Central Depression. The origin of these morphostructural units has been largely debated. They have been ascribed to different ages between the Cretaceous and the Pliocene, and tectonic causes that involve extensional or contractional deformation and have been related to Andean evolution or accretionary processes in the forearc. To investigate the geologic evolution of the Coastal Cordillera and Central Depression in the cited area, we based on stratigraphy, sedimentology, geochronology (U[sbnd]Pb, LA-ICP-MS), structural geology, and geomorphology. Our studies, which are based on our own data and a thorough bibliographic review, indicate the following sequence of events in the tectono-sedimentary evolution of the forearc of south-central Chile during the late Cenozoic. The area was subjected to extensional tectonics caused by slab rollback during the Oligocene-early Miocene, resulting in the genesis of a series of basins filled with volcanic, continental, and marine deposits that extended from the present Chilean coast to the retroac. The extensional basins were tectonically inverted during the late Early-Late Miocene, and most of the forearc became a positive relief that was subjected to fluvial erosion. The West Andean Thrust, a major reverse west verging fault in the limit between the Central Depression and the Andean Cordillera, caused significant uplift of the western flank of the Andes. This triggered flexural subsidence and the accumulation of coarse-grained deposits in the forearc during the Pliocene-Pleistocene. The sudden increase of sediment flux to the trench during this period caused a change from non-accretion or subduction erosion to accretion in the margin of south-central Chile. Progressive growth of the accretionary wedge gave birth to a forearc high, the Coastal Cordillera, which was uplifted in the last 2 Ma. Some forearc areas placed above subducted oceanic fracture zones did not experience uplift during this period due to the significant transport of fluid that inhibited the deformation of the accretionary wedge. Our studies show that the late Cenozoic tectono-sedimentary evolution of the forearc in the study area is complex and involves different processes related to Andean evolution in the first stage and accretionary processes acting exclusively in the forearc in a second stage. The Coastal Cordillera and the Central Depression of south-central Chile were developed in the last 2 Ma and are much younger than classically considered.Fil: Encinas, A.. Universidad de Concepción; ChileFil: Sagripanti, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Estudios Andinos "Don Pablo Groeber". Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Estudios Andinos "Don Pablo Groeber"; ArgentinaFil: Rodríguez, M.P.. Universidad de Atacama.; ChileFil: Orts, Darío Leandro. Universidad Nacional de Río Negro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación en Paleobiología y Geología; ArgentinaFil: Anavalón, A.. Universidad de Concepción; ChileFil: Giroux, P.. Universidad de Concepción; ChileFil: Otero, J.. Universidad Austral de Chile; ChileFil: Echaurren Gonzalez, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Provincia de Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Universidad Nacional de Cuyo. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales; ArgentinaFil: Zambrano, P.. Universidad Andrés Bello; ChileFil: Valencia, V.. Washington State University; Estados Unido

Increased Activities against Biofilms of the Pathogenic Yeast Candida albicans of Optimized Pom-1 Derivatives

Antimicrobial peptides (AMPs) are an alternative group for the therapy of infectious diseases, with activity against a wide range of diverse pathogens. However, classical AMPs have significant side effects in human cells due to their unspecific pore formation in biomembranes. Nevertheless, AMPs are promising therapeutics and can be isolated from natural sources, which include sea and freshwater molluscs. The AMPs identified in these organisms show promising antimicrobial activities, as pathogens are mainly fought by innate defence mechanisms. An auspicious candidate among molluscs is the Cuban freshwater snail Pomacea poeyana, from which the peptides Pom-1 and Pom-2 have been isolated and studied. These studies revealed significant antimicrobial activities for both AMPs. Based on the activities determined, Pom-1 was used for further optimization. In order to meet the emerging requirements of improved anti-biofilm activity against naturally occurring Candida species, the six derivatives Pom-1A to F were developed and investigated. Analysis of the derivatives acting on the most abundant naturally occurring Candida yeast Candida albicans (C. albicans) revealed a strong anti-biofilm activity, especially induced by Pom-1 B, C, and D. Furthermore, a moderate decrease in the metabolic activity of planktonic yeast cells was observed

Bottom-up control of common octopus Octopus vulgaris in the Galician upwelling system, northeast Atlantic Ocean

This paper investigates the possible underlying causes of the wide interannual fluctuations in catch of the common octopus Octopus vulgaris Cuvier, 1797 in one of the main small-scale fisheries off the coast of Galicia (northwest Spain). Galicia is at the northern boundary of the Iberian–Canary current upwelling system in the northeast Atlantic Ocean, where local winds induce seasonal upwelling, largely driving the annual cycles of primary and secondary production. We hypothesize that such dynamics are also fundamental for the survival of the planktonic stages of octopus and set the year class strength. We address this hypothesis by investigating the influence of upwelling on time-series of octopus fishery data. Wind stress structure during the spring–summer (prior to the hatching peak) and autumn–winter (during the planktonic stage) was found to affect the early life phase of this species, and explains up to 85% of the total variance of the year-to-year variability of the adult catch. Despite this bottom-up modulation via environmental conditions, our results also provide evidence for a between-cohort density-dependent interaction, probably caused by cannibalism and competition for habitat.Postprin

Status and challenges of plant-anticancer compounds in cancer treatment

Nowadays, cancer is one of the deadliest diseases in the world, which has been estimated to cause 9.9 million deaths in 2020. Conventional treatments for cancer commonly involve monochemotherapy or a combination of radiotherapy and mono-chemotherapy. However, the negative side effects of these approaches have been extensively reported and have prompted the search of new therapeutic drugs. In this context, scientific community started to look for innovative sources of anticancer compounds in natural sources, including traditional plants. Currently, numerous studies have evaluated the anticancer properties of natural compounds derived from plants, both in vitro and in vivo. In pre-clinical stages, some promising compounds could be mentioned, such as the sulforaphane or different phenolic compounds. On the other hand, some phytochemicals obtained positive results in clinical stages and were further approved for cancer treatment, such as vinca alkaloids or the paclitaxel. Nevertheless, these compounds are not exempt of limitations, such as low solubility, restricted effect on their own, negative side-effects, etc. This review aims to compile the information about the current phytochemicals used for cancer treatment and also promising candidates, main action mechanisms and also reported limitations. In this sense, some strategies to face the limitations have been considered, such as nano-based formulations to improve solubility or chemical modification to reduce toxicity. In conclusion, although more research is still necessary to develop more efficient and safe phytochemical drugs, more of these compounds might be used in future cancer therapies.The research leading to these results was supported by: MICINN supporting the Ramón&Cajal grant for M.A. Prieto (RYC-2017-22891), the project UP4HEALTH for supporting the researcher P. Otero, the Xunta de Galicia and University of Vigo supporting the post-doctoral grant for M. Fraga-Corral (ED481B-2019/096) and the pre-doctoral grants for P. García-Oliveira (ED481A-2019/295) and A.G. Pereira (ED481A-2019/0228).info:eu-repo/semantics/publishedVersio

Combination of Six Individual Derivatives of the Pom-1 Antibiofilm Peptide Doubles Their Efficacy against Invasive and Multi-Resistant Clinical Isolates of the Pathogenic Yeast Candida albicans

In previous studies, derivatives of the peptide Pom-1, which was originally extracted from the freshwater mollusk Pomacea poeyana, showed an exceptional ability to specifically inhibit biofilm formation of the laboratory strain ATCC 90028 as a model strain of the pathogenic yeast Candida albicans. In follow-up, here, we demonstrate that the derivatives Pom-1A to Pom-1F are also active against biofilms of invasive clinical C. albicans isolates, including strains resistant against fluconazole and/or amphotericin B. However, efficacy varied strongly between the isolates, as indicated by large deviations in the experiments. This lack of robustness could be efficiently bypassed by using mixtures of all peptides. These mixed peptide preparations were active against biofilm formation of all the isolates with uniform efficacies, and the total peptide concentration could be halved compared to the original MIC of the individual peptides (2.5 µg/mL). Moreover, mixing the individual peptides restored the antifungal effect of fluconazole against fluconazole-resistant isolates even at 50% of the standard therapeutic concentration. Without having elucidated the reason for these synergistic effects of the peptides yet, both the gain of efficacy and the considerable increase in efficiency by combining the peptides indicate that Pom-1 and its derivatives in suitable formulations may play an important role as new antibiofilm antimycotics in the fight against invasive clinical infections with (multi-) resistant C. albicans

Exploring the Pharmacological Potential of Promiscuous Host-Defense Peptides: From Natural Screenings to Biotechnological Applications

In the last few years, the number of bacteria with enhanced resistance to conventional antibiotics has dramatically increased. Most of such bacteria belong to regular microbial flora, becoming a real challenge, especially for immune-depressed patients. Since the treatment is sometimes extremely expensive, and in some circumstances completely inefficient for the most severe cases, researchers are still determined to discover novel compounds. Among them, host-defense peptides (HDPs) have been found as the first natural barrier against microorganisms in nearly all living groups. This molecular class has been gaining attention every day for multiple reasons. For decades, it was believed that these defense peptides had been involved only with the permeation of the lipid bilayer in pathogen membranes, their main target. Currently, it is known that these peptides can bind to numerous targets, as well as lipids including proteins and carbohydrates, from the surface to deep within the cell. Moreover, by using in vivo models, it was shown that HDPs could act both in pathogens and cognate hosts, improving immunological functions as well as acting through multiple pathways to control infections. This review focuses on structural and functional properties of HDP peptides and the additional strategies used to select them. Furthermore, strategies to avoid problems in large-scale manufacture by using molecular and biochemical techniques will also be explored. In summary, this review intends to construct a bridge between academic research and pharmaceutical industry, providing novel insights into the utilization of HDPs against resistant bacterial strains that cause infections in humans

Immigration and Sexual Partner Risk Among Latino Adolescents in San Francisco

Sexual partner characteristics increase risk for adverse reproductive health outcomes. Evidence is limited regarding whether choice of sexual partners among Latino adolescents changes with U.S. acculturation/adaptation