A Learning Approach to Monitoring and Evaluation

This article draws on literature from both monitoring and evaluation (M&E) and organisational learning to explore synergies between these two fields in support of organisational performance. Two insights from the organisational learning literature are that organisations learn through ‘double?loop’ learning: reflecting on experience and using this to question critically underlying assumptions; and that power relations within an organisation will influence what and whose learning is valued and shared. This article identifies four incentives that can help link M&E with organisational learning: the incentive to learn why; the incentive to learn from below; the incentive to learn collaboratively; and the incentive to take risks. Two key elements are required to support these incentives: (1) establishing and promoting an ‘evaluative culture’ within an organisation; and (2) having accountability relationships where value is placed on learning ‘why’, as well as on learning from mistakes, which requires trust

Devotions for Lent 2023 Hymns of Lent

This Lent, we will continue reflecting on hymns of faith, namely, some of our most beloved Lenten hymns. 10 such hymns have been chosen to fill the 40(+) days of Lent. Therefore, this devotional, different from previous editions, does not proceed on a weekly basis, but merely flows from one hymn to the next. Also different from previous editions, the devotional reflections are specifically based on the stanzas of the selected hymns. Therefore, each day’s reflection features the text of the hymn stanza, a devotion based on that stanza, a prayer, and then a Scripture passage or passages for further meditation. I pray these reflections may be of edification for you during this Lenten season.https://scholar.csl.edu/osp/1022/thumbnail.jp

Fumonisins affect the intestinal microbial homeostasis in broiler chickens, predisposing to necrotic enteritis

Fumonisins (FBs) are mycotoxins produced by Fusarium fungi. This study aimed to investigate the effect of these feed contaminants on the intestinal morphology and microbiota composition, and to evaluate whether FBs predispose broilers to necrotic enteritis. One-day-old broiler chicks were divided into a group fed a control diet, and a group fed a FBs contaminated diet (18.6 mg FB1+ FB2/kg feed). A significant increase in the plasma sphinganine/sphingosine ratio in the FBs-treated group (0.21 +/- 0.016) compared to the control (0.14 +/- 0.014) indicated disturbance of the sphingolipid biosynthesis. Furthermore, villus height and crypt depth of the ileum was significantly reduced by FBs. Denaturing gradient gel electrophoresis showed a shift in the microbiota composition in the ileum in the FBs group compared to the control. A reduced presence of low-GC containing operational taxonomic units in ileal digesta of birds exposed to FBs was demonstrated, and identified as a reduced abundance of Candidatus Savagella and Lactobaccilus spp. Quantification of total Clostridium perfringens in these ileal samples, previous to experimental infection, using cpa gene (alpha toxin) quantification by qPCR showed an increase in C. perfringens in chickens fed a FBs contaminated diet compared to control (7.5 +/- 0.30 versus 6.3 +/- 0.24 log10 copies/g intestinal content). After C. perfringens challenge, a higher percentage of birds developed subclinical necrotic enteritis in the group fed a FBs contaminated diet as compared to the control (44.9 +/- 2.22% versus 29.8 +/- 5.46%)

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Lamenting Toward Hope: Peculiar Speech in a Changing World

The ways the church speaks shape her hope. While the church uses many forms of speaking, she has not often used the biblical language of lament in worship or preaching. Too often her hope is stunted. A rapidly changing context allows space for the recovery of lament in corporate worship and preaching, allowing the church to clarify her hope. Recovering a biblical language of lament would allow the church to aim her hope even more sharply, rather than, as Walter Brueggemann puts it, “…to accept the world’s possibilities as the only chance for the future.” This sectional will explore the biblical and theological themes of lament, provide options for a Lutheran theology of lament and propose a regular practice of lament in worship and preaching. Specific attention will be given to the corporate practice of lament. Through these practices, the church can shape her language and her hope toward “the resurrection of the dead and the life of the world to come.

101. Genesis 3:1-21

Chapel Sermon by Drew Oswald from Genesis 3:1-21 on Thursday, March 2, 2023

Miscarriage and Memorial Service 10/27/22

Sample Bulletin and Entire Service Recorded for the Miscarriage and Memorial Service 10/27/22. Sermon only can be found at Chapel Sermon.https://scholar.csl.edu/lifeteam/1000/thumbnail.jp

The Burr November 2012

https://kent-islandora.s3.us-east-2.amazonaws.com/theburr/54/thumbnail.jp

Structure of the aliphatic sulfonate-binding protein SsuA from Escherichia coli

Sulfur is an essential component for the biosynthesis of the sulfur-containing amino acids l-methionine and l-cysteine. The crystal structure of the periplasmic aliphatic sulfonate-binding protein SsuA from E. coli has been solved at 1.75 Å resolution in a substrate-free state. Comparison with the substrate-bound protein shows significant movement of one of the two domains