Protective effects of halite to vacuum and vacuum-ultraviolet radiation: A potential scenario during a young sun superflare

Halite (NaCl mineral) has exhibited the potential to preserve microorganisms for millions of years on Earth. This mineral was also identified on Mars and in meteorites. In this study, we investigated the potential of halite crystals to protect microbial life-forms on the surface of an airless body (e.g., meteorite), for instance, during a lithopanspermia process (interplanetary travel step) in the early Solar System. To investigate the effect of the radiation of the young Sun on microorganisms, we performed extensive simulation experiments by employing a synchrotron facility. We focused on two exposure conditions: vacuum (low Earth orbit, 10-4 Pa) and vacuum-ultraviolet (VUV) radiation (range 57.6-124 nm, flux 7.14 W/m2), with the latter representing an extreme scenario with high VUV fluxes comparable to the amount of radiation of a stellar superflare from the young Sun. The stellar VUV parameters were estimated by using the very well-studied solar analog of the young Sun, κ1 Cet. To evaluate the protective effects of halite, we entrapped a halophilic archaeon (Haloferax volcanii) and a non-halophilic bacterium (Deinococcus radiodurans) in laboratory-grown halite. Control groups were cells entrapped in salt crystals (mixtures of different salts and NaCl) and non-trapped (naked) cells, respectively. All groups were exposed either to vacuum alone or to vacuum plus VUV. Our results demonstrate that halite can serve as protection against vacuum and VUV radiation, regardless of the type of microorganism. In addition, we found that the protection is higher than provided by crystals obtained from mixtures of salts. This extends the protective effects of halite documented in previous studies and reinforces the possibility to consider the crystals of this mineral as potential preservation structures in airless bodies or as vehicles for the interplanetary transfer of microorganisms.X.C.A. acknowledges CNPEM for the beamtime grantedto the proposal TGM—16126 (LNLS), FAPESP postdoc-toral fellowship (years 2013–2014) (Processo nro: 2012/20106-5), Brazil, and funding from PIP—CONICET 0754,Argentina. M.L. and P.O. acknowledge the Austrian ScienceFund (FWF): P30949-N36, I5711-N for supporting thisproject. J.E.H. acknowledges the financial support of FAPESP (Sao Paulo State) and CNPQ (Brazil) financingagencies. G.F.P.M. acknowledges grant 474972/2009-7from CNPq/Brazil.Peer reviewe

Dose fractionation as a promising strategy to improve UV disinfection

Survival curves for bacteria exposed to germicidal radiation (254 nm) usually exhibit a decreased slope at high doses, because bacterial populations contain a small proportion of cells able to tolerate the effects of the radiation. These cells could survive due to external shielding by aggregation or association with inert particles. However, it has been proposed that transient and reversible phenotypic changes modifying UV susceptibility also occur and lead to a phenomenon of persistence. Using Escherichia coli as a model organism, and comparing the effects of continuous exposures to those of irradiations separated by incubations in the dark, we found that in most of the survivors to high UV doses, the condition conferring tolerance (possibly self-aggregation) is transient and reversible, as would be expected in a persistent sub-population. Splitting the irradiations into two exposures reduced viable counts to the detection limit (6.32 orders of magnitude) under conditions in which continuous irradiations produced a reduction no greater than 4 orders of magnitude. In those processes where it is feasible, UV dose fractionation could improve the effectiveness of disinfection by ultraviolet radiation

Anr, the anaerobic global regulator, modulates the redox state and oxidative stress resistance in Pseudomonas extremaustralis

The role of Anr in oxidative stress resistance was investigated in Pseudomonas extremaustralis, a polyhydroxybutyrate-producing Antarctic bacterium. The absence of Anr caused increased sensitivity to hydrogen peroxide under low oxygen tension. This phenomenon was associated with a decrease in the redox ratio, higher oxygen consumption and higher reactive oxygen species production. Physiological responses of the mutant to the oxidized state included an increase in NADP(H) content, catalase activity and exopolysaccharide production. The wild-type strain showed a sharp decrease in the reduced thiol pool when exposed to hydrogen peroxide, not observed in the mutant strain. In silico analysis of the genome sequence of P. extremaustralis revealed putative Anr binding sites upstream from genes related to oxidative stress. Genes encoding several chaperones and cold shock proteins, a glutathione synthase, a sulfate transporter and a thiol peroxidase were identified as potential targets for Anr regulation. Our results suggest a novel role for Anr in oxidative stress resistance and in redox balance maintenance under conditions of restricted oxygen supply.Fil: Tribelli, Paula Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Nikel, Pablo Ivan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Oppezzo, Oscar J.. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Departamento de Radiobiología; ArgentinaFil: López, Nancy I.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentin