Synthesis and structural characterization of thin multi-walled carbon nanotubes with a partially facetted cross section by a floating reactant method

ArticleCarbon. 43(11):2243-2250 (2005)journal articl

Removal of entrapped iron compounds from isothermally treated catalytic chemical vapor deposition derived multi-walled carbon nanotubes

ArticleCARBON. 46(3): 391-396(2008)journal articl

Cosmology from weak lensing peaks and minima with Subaru Hyper Suprime-Cam survey first-year data

We present cosmological constraints derived from peak counts, minimum counts, and the angular power spectrum of the Subaru Hyper Suprime-Cam first-year (HSC Y1) weak lensing shear catalog. Weak lensing peak and minimum counts contain non-Gaussian information and hence are complementary to the conventional two-point statistics in constraining cosmology. In this work, we forward-model the three summary statistics and their dependence on cosmology, using a suite of $N$-body simulations tailored to the HSC Y1 data. We investigate systematic and astrophysical effects including intrinsic alignments, baryon feedback, multiplicative bias, and photometric redshift uncertainties. We mitigate the impact of these systematics by applying cuts on angular scales, smoothing scales, statistic bins, and tomographic redshift bins. By combining peaks, minima, and the power spectrum, assuming a flat-$\Lambda$CDM model, we obtain $S_{8} \equiv \sigma_8\sqrt{\Omega_m/0.3}= 0.810^{+0.022}_{-0.026}$, a 35\% tighter constraint than that obtained from the angular power spectrum alone. Our results are in agreement with other studies using HSC weak lensing shear data, as well as with Planck 2018 cosmology and recent CMB lensing constraints from the Atacama Cosmology Telescope and the South Pole Telescope

The Hyper Suprime-Cam SSP Survey: Overview and Survey Design

Hyper Suprime-Cam (HSC) is a wide-field imaging camera on the prime focus of the 8.2m Subaru telescope on the summit of Maunakea in Hawaii. A team of scientists from Japan, Taiwan and Princeton University is using HSC to carry out a 300-night multi-band imaging survey of the high-latitude sky. The survey includes three layers: the Wide layer will cover 1400 deg$^2$ in five broad bands ($grizy$), with a $5\,\sigma$ point-source depth of $r \approx 26$. The Deep layer covers a total of 26~deg$^2$ in four fields, going roughly a magnitude fainter, while the UltraDeep layer goes almost a magnitude fainter still in two pointings of HSC (a total of 3.5 deg$^2$). Here we describe the instrument, the science goals of the survey, and the survey strategy and data processing. This paper serves as an introduction to a special issue of the Publications of the Astronomical Society of Japan, which includes a large number of technical and scientific papers describing results from the early phases of this survey.Comment: 14 pages, 7 figures, 5 tables. Corrected for a typo in the coordinates of HSC-Wide spring equatorial field in Table

HSC Year 1 cosmology results with the minimal bias method: HSC×\timesBOSS galaxy-galaxy weak lensing and BOSS galaxy clustering

We present cosmological parameter constraints from a blinded joint analysis of galaxy-galaxy weak lensing, $\Delta\!\Sigma(R)$, and projected correlation function, $w_\mathrm{p}(R)$, measured from the first-year HSC (HSC-Y1) data and SDSS spectroscopic galaxies over $0.15<z<0.7$. We use luminosity-limited samples as lens samples for $\Delta\!\Sigma$ and as large-scale structure tracers for $w_\mathrm{p}$ in three redshift bins, and use the HSC-Y1 galaxy catalog to define a secure sample of source galaxies at $z_\mathrm{ph}>0.75$ for the $\Delta\!\Sigma$ measurements, selected based on their photometric redshifts. For theoretical template, we use the "minimal bias" model for the cosmological clustering observables for the flat $\Lambda$CDM cosmological model. We compare the model predictions with the measurements in each redshift bin on large scales, $R>12$ and $8~h^{-1}\mathrm{Mpc}$ for $\Delta\!\Sigma(R)$ and $w_\mathrm{p}(R)$, respectively, where the perturbation theory-inspired model is valid. When we employ weak priors on cosmological parameters, without CMB information, we find $S_8=0.936^{+0.092}_{-0.086}$, $\sigma_8=0.85^{+0.16}_{-0.11}$, and $\Omega_\mathrm{m}=0.283^{+0.12}_{-0.035}$ for the flat $\Lambda$CDM model. Although the central value of $S_8$ appears to be larger than those inferred from other cosmological experiments, we find that the difference is consistent with expected differences due to sample variance, and our results are consistent with the other results to within the statistical uncertainties. (abriged)Comment: 24 pages, 19 figures, 4 tables, to be submitted to Phys. Rev.

PlantNATsDB: a comprehensive database of plant natural antisense transcripts

Natural antisense transcripts (NATs), as one type of regulatory RNAs, occur prevalently in plant genomes and play significant roles in physiological and pathological processes. Although their important biological functions have been reported widely, a comprehensive database is lacking up to now. Consequently, we constructed a plant NAT database (PlantNATsDB) involving approximately 2 million NAT pairs in 69 plant species. GO annotation and high-throughput small RNA sequencing data currently available were integrated to investigate the biological function of NATs. PlantNATsDB provides various user-friendly web interfaces to facilitate the presentation of NATs and an integrated, graphical network browser to display the complex networks formed by different NATs. Moreover, a ‘Gene Set Analysis’ module based on GO annotation was designed to dig out the statistical significantly overrepresented GO categories from the specific NAT network. PlantNATsDB is currently the most comprehensive resource of NATs in the plant kingdom, which can serve as a reference database to investigate the regulatory function of NATs. The PlantNATsDB is freely available at http://bis.zju.edu.cn/pnatdb/

Immune-Complex Mimics as a Molecular Platform for Adjuvant-Free Vaccine Delivery

Protein-based vaccine development faces the difficult challenge of finding robust yet non-toxic adjuvants suitable for humans. Here, using a molecular engineering approach, we have developed a molecular platform for generating self-adjuvanting immunogens that do not depend on exogenous adjuvants for induction of immune responses. These are based on the concept of Immune Complex Mimics (ICM), structures that are formed between an oligomeric antigen and a monoclonal antibody (mAb) to that antigen. In this way, the roles of antigens and antibodies within the structure of immune complexes are reversed, so that a single monoclonal antibody, rather than polyclonal sera or expensive mAb cocktails can be used. We tested this approach in the context of Mycobacterium tuberculosis (MTB) infection by linking the highly immunogenic and potentially protective Ag85B with the oligomeric Acr (alpha crystallin, HspX) antigen. When combined with an anti-Acr monoclonal antibody, the fusion protein formed ICM which bound to C1q component of the complement system and were readily taken up by antigen-presenting cells in vitro. ICM induced a strong Th1/Th2 mixed type antibody response, which was comparable to cholera toxin adjuvanted antigen, but only moderate levels of T cell proliferation and IFN-γ secretion. Unfortunately, the systemic administration of ICM did not confer statistically significant protection against intranasal MTB challenge, although a small BCG-boosting effect was observed. We conclude that ICM are capable of inducing strong humoral responses to incorporated antigens and may be a suitable vaccination approach for pathogens other than MTB, where antibody-based immunity may play a more protective role

Integrative analysis of RUNX1 downstream pathways and target genes

Background: The RUNX1 transcription factor gene is frequently mutated in sporadic myeloid and lymphoid leukemia through translocation, point mutation or amplification. It is also responsible for a familial platelet disorder with predisposition to acute myeloid leukemia (FPD-AML). The disruption of the largely unknown biological pathways controlled by RUNX1 is likely to be responsible for the development of leukemia. We have used multiple microarray platforms and bioinformatic techniques to help identify these biological pathways to aid in the understanding of why RUNX1 mutations lead to leukemia. Results: Here we report genes regulated either directly or indirectly by RUNX1 based on the study of gene expression profiles generated from 3 different human and mouse platforms. The platforms used were global gene expression profiling of: 1) cell lines with RUNX1 mutations from FPD-AML patients, 2) over-expression of RUNX1 and CBF[Beta], and 3) Runx1 knockout mouse embryos using either cDNA or Affymetrix microarrays. We observe that our datasets (lists of differentially expressed genes) significantly correlate with published microarray data from sporadic AML patients with mutations in either RUNX1 or its cofactor, CBF[Beta]. A number of biological processes were identified among the differentially expressed genes and functional assays suggest that heterozygous RUNX1 point mutations in patients with FPD-AML impair cell proliferation, microtubule dynamics and possibly genetic stability. In addition, analysis of the regulatory regions of the differentially expressed genes has for the first time systematically identified numerous potential novel RUNX1 target genes. Conclusion: This work is the first large-scale study attempting to identify the genetic networks regulated by RUNX1, a master regulator in the development of the hematopoietic system and leukemia. The biological pathways and target genes controlled by RUNX1 will have considerable importance in disease progression in both familial and sporadic leukemia as well as therapeutic implications

Expression pattern of four storage xyloglucan mobilization-related genes during seedling development of the rain forest tree Hymenaea courbaril L.

During seedling establishment, cotyledons of the rain forest tree Hymenaea courbaril mobilize storage cell wall xyloglucan to sustain growth. The polysaccharide is degraded and its products are transported to growing sink tissues. Auxin from the shoot controls the level of xyloglucan hydrolytic enzymes. It is not yet known how important the expression of these genes is for the control of storage xyloglucan degradation. In this work, partial cDNAs of the genes xyloglucan transglycosylase hydrolase (HcXTH1) and β-galactosidase (HcBGAL1), both related to xyloglucan degradation, and two other genes related to sucrose metabolism [alkaline invertase (HcAlkIN1) and sucrose synthase (HcSUS1)], were isolated. The partial sequences were characterized by comparison with sequences available in the literature, and phylogenetic trees were assembled. Gene expression was evaluated at intervals of 6 h during 24 h in cotyledons, hypocotyl, roots, and leaves, using 45-d-old plantlets. HcXTH1 and HcBGAL1 were correlated to xyloglucan degradation and responded to auxin and light, being down-regulated when transport of auxin was prevented by N-1-naphthylphthalamic acid (NPA) and stimulated by constant light. Genes related to sucrose metabolism, HcAlkIN1 and HcSUS1, responded to inhibition of auxin transport in consonance with storage mobilization in the cotyledons. A model is proposed suggesting that auxin and light are involved in the control of the expression of genes related to storage xyloglucan mobilization in seedlings of H. courbaril. It is concluded that gene expression plays a role in the control of the intercommunication system of the source–sink relationship during seeding growth, favouring its establishment in the shaded environment of the rain forest understorey

A novel RUNX2 missense mutation predicted to disrupt DNA binding causes cleidocranial dysplasia in a large Chinese family with hyperplastic nails

Background: Cleidocranial dysplasia (CCD) is a dominantly inherited disease characterized by hypoplastic or absent clavicles, large fontanels, dental dysplasia, and delayed skeletal development. The purpose of this study is to investigate the genetic basis of Chinese family with CCD. Methods: Here, a large Chinese family with CCD and hyperplastic nails was recruited. The clinical features displayed a significant intrafamilial variation. We sequenced the coding region of the RUNX2 gene for the mutation and phenotype analysis. Results: The family carries a c. T407C (p.L136P) mutation in the DNA- and CBF beta-binding Runt domain of RUNX2. Based on the crystal structure, we predict this novel missense mutation is likely to disrupt DNA binding by RUNX2, and at least locally affect the Runt domain structure. Conclusion: A novel missense mutation was identified in a large Chinese family with CCD with hyperplastic nails. This report further extends the mutation spectrum and clinical features of CCD. The identification of this mutation will facilitate prenatal diagnosis and preimplantation genetic diagnosis