Distribution of Polysulfide in Human Biological Fluids and Their Association with Amylase and Sperm Activities

Intracellular polysulfide could regulate the redox balance via its anti-oxidant activity. However, the existence of polysulfide in biological fluids still remains unknown. Recently, we developed a quantitative analytical method for polysulfide and discovered that polysulfide exists in plasma and responds to oxidative stress. In this study, we confirmed the presence of polysulfide in other biological fluids, such as semen and nasal discharge. The levels of polysulfide in these biological fluids from healthy volunteers (n = 9) with identical characteristics were compared. Additionally, the circadian rhythm of plasma polysulfide was also investigated. The polysulfide levels detected from nasal discharge and seminal fluid were approximately 400 and 600 μM, respectively. No correlation could be found between plasma polysulfide and the polysulfide levels of tear, saliva, and nasal discharge. On the other hand, seminal polysulfide was positively correlated with plasma polysulfide, and almost all polysulfide contained in semen was found in seminal fluid. Intriguingly, saliva and seminal polysulfide strongly correlated with salivary amylase and sperm activities, respectively. These results provide a foundation for scientific breakthroughs in various research areas like infertility and the digestive system process

Distribution of Polysulfide in Human Biological Fluids and Their Association with Amylase and Sperm Activities

Intracellular polysulfide could regulate the redox balance via its anti-oxidant activity. However, the existence of polysulfide in biological fluids still remains unknown. Recently, we developed a quantitative analytical method for polysulfide and discovered that polysulfide exists in plasma and responds to oxidative stress. In this study, we confirmed the presence of polysulfide in other biological fluids, such as semen and nasal discharge. The levels of polysulfide in these biological fluids from healthy volunteers (n = 9) with identical characteristics were compared. Additionally, the circadian rhythm of plasma polysulfide was also investigated. The polysulfide levels detected from nasal discharge and seminal fluid were approximately 400 and 600 μM, respectively. No correlation could be found between plasma polysulfide and the polysulfide levels of tear, saliva, and nasal discharge. On the other hand, seminal polysulfide was positively correlated with plasma polysulfide, and almost all polysulfide contained in semen was found in seminal fluid. Intriguingly, saliva and seminal polysulfide strongly correlated with salivary amylase and sperm activities, respectively. These results provide a foundation for scientific breakthroughs in various research areas like infertility and the digestive system process

A Facelift Procedure for Resection of Benign Parapharyngeal Tumors

Objective: The feasibility, surgical outcomes and possible risks and complications encountered during a facelift procedure for patients with parapharyngeal space (PPS) tumor were analyzed. Method: This retrospective analysis examined 10 patients who underwent surgery for PPS tumor using a facelift incision at our institutes between April 2015 and August 2019. Results: This study included four retro-styloid (benign nerve sheath tumor) and six pre-styloid tumors (pleomorphic adenoma). Mean tumor dimensions were 4.1 x 4.2 x 3.8 cm respectively. None of the patients needed conversion to conventional open resection. Transient sensory changes in the auricle occurred in 30% of the patients; however, all recovered within four months. In all the patients, postoperative scars were fully concealed by the auricle and hair. No recurrences were detected during a mean follow-up period of 16.6 months. Conclusion: The facelift procedure provides adequate visualization, workspace and excellent cosmetic results in properly selected cases

Lifting twarzy w resekcji łagodnych nowotworów przestrzeni przygardłowej

Cel: Przeanalizowano opcje terapeutyczne, wyniki leczenia, potencjalne ryzyko i powikłania w czasie zabiegu liftingu twarzy u pacjentów z guzami przestrzeni przygardłowej (PPG). Metody: W retrospektywnej analizie uwzględniono 10 pacjentów poddawanych leczeniu operacyjnemu w naszym ośrodku w okresie od kwietnia 2015 r. do sierpnia 2019 r. z powodu guza PPG z wykorzystaniem cięcia do liftingu. Wyniki: W badaniu uwzględniono cztery guzy zarylcowate (łagodne nowotwory z osłonek nerwów obwodowych) i sześć guzów przedrylcowatych (gruczolak wielopostaciowy). Średnie wymiary guza wynosiły 4,1 x 4,2 x 3,8 cm. U żadnego pacjenta nie zaistniała potrzeba konwersji do otwartej resekcji. Przejściowe zaburzenia czucia w obrębie płatka pojawiły się u 30% osób, przy czym u wszystkich zmiany wycofały się w ciągu czterech miesięcy. U pacjentów ranę pooperacyjną całkowicie zakrywała małżowina uszna i włosy. Nie obserwowano wznowy w okresie obserwacji przez średnio 16.6 miesięcy. Wnioski: Lifting twarzy zapewnia dobre uwidocznienie guza, właściwe pole operacyjne i doskonałe efekty kosmetyczne u wybranych chorych

Single Short Retention Instillation of Pirarubicin Prevents Intravesical Recurrence of Low-risk Non Muscle Invasive Bladder Cancer.

Background:This study evaluated the efficacy of a single instillation of pirarubicin with a short retention time for preventing intravesical recurrence of low-risk non-muscle-invasive bladder cancer.Patients and methods:We analyzed 165 patients with low-risk non-muscle-invasive bladder cancer who underwent transurethral surgery. Single instillation of pirarubicin with 15-min retention time immediate after surgery was performed in 47 (28%) patients. The other patients (118, 72%) were treated without instillation therapy. The primary endpoint was recurrence-free survival.Results:Median overall follow-up was 50 (range=6-134) months. Recurrence-free survival at 1 and 5 years was 91% and 72%, and 79% and 54% in the group treated with pirarubicin, and that treated with surgery alone, respectively (p=0.031). Cox\u27s hazard analysis revealed lack of instillation and larger tumor size (>10 mm) as significant factors for risk of recurrence. No adverse events regarding intravesical chemotherapy were observed.Conclusion:Pirarubicin instillation with 15-min retention time can prevent intravesical recurrence of low-risk bladder tumors