PROYECTO DE NOVELA GRÁFICA

[EN] This memory contains a graphic novel’s preproduction. The story is about an adventure in a fictional world where the real and the magical are mixed. This work has been developed with a colleague, who participated in the script and the mythology of the story. The intention is to graphically expand the first moments and elements of the comic. This includes the main characters of the story, some backgrounds of the places where the first part of the story takes place and the more important gods of the mythology. Moreover, the storyboard of the first volume is developed including its initial pages. The intentionality of this work is to have a first approach of the project, that will keep on evolving in a near future until it is a solid and final project, that can be auto-edited or be presented to publishers[ES] Esta memoria recoge la preproducción de una novela gráfica. La historia trata de una aventura, en un mundo ficticio, donde se mezcla lo real y lo mágico. La colaboración de un estudiante de historia ha ayudado ha desarrollar el mundo imaginario donde transcurre las acciones. Gracias a esta colaboración se ha podido desarrollar la trama de la historia de manera creíble y compleja. El objetivo principal ha sido desarrollar de manera gráfica los primeros elementos del cómic. Estos elementos son los personajes principales de la historia, algunos fondos del lugar donde transcurre la primera parte de la trama y los dioses más importantes de la mitología. Además, se ha realizado el storyboard del primer tomo y las primeras páginas de éste. La intención de este trabajo es tener los elementos necesarios para que en un futuro seguir trabajando con este proyecto y poder concluirlo para una futura autoedición o presentar a editorialesOrtiz Masia, A. (2016). PROYECTO DE NOVELA GRÁFICA. http://hdl.handle.net/10251/76254.TFG

Análisis de la docencia en línea en el proceso enseñanza-aprendizaje: efectos colaterales del SARS-COV2 en la asignatura Fundamentos Médicos del Máster Ingeniería Biomédica

[EN] The current health situation caused by SARS-CoV2 has forced the adaptation of university teaching, favoring continuous assessment and the transition from classroom teaching to no classroom teaching. In the present work we analyze and compare the continuous and final evaluation grades in the pre-pandemic period (academic year 2018-2019) with the pandemic period (academic year 2019-2020) of the subject Medical Fundamentals of the Master's Degree in Biomedical Engineering. In addition, the students' perception of the online methodology was analyzed by means of the official evaluation surveys of the faculty. The results show that in the subject Medical Fundamentals, the change to online teaching led to a significant improvement in the continuous evaluation, in the final evaluation and in the final grade of the subject. However, the students' perception showed an inverse tendency, showing a dislike of the teaching methodology and the online evaluation carried out.[ES] La actual situación sanitaria producida por el SARS-CoV2 ha forzado la adaptación de la docencia universitaria, favoreciendo la evaluación continua y el paso de la docencia presencial a docencia no presencial. En el presente trabajo se analizan y comparan las calificaciones de evaluación continua y final en el periodo pre-pandemia (curso 2018-2019) con el periodo pandemia (curso 2019-2020) de la asignatura Fundamentos Médicos, del Máster de Ingeniería Biomédica. Además, se analizó la percepción de los estudiantes a la metodología en línea mediante las encuestas oficiales de evaluación del profesorado. Los resultados muestran que en la asignatura Fundamentos Médicos, el paso a la docencia en línea supuso una mejora significativa en la evaluación continua, en la evaluación final y en la calificación final de la asignatura. Sin embargo, la percepción de los estudiantes mostraba tendencia inversas, mostrando un desagrado en la metodología docente y la evaluación en línea realizada.Ortiz-Masia, D. (2021). Análisis de la docencia en línea en el proceso enseñanza-aprendizaje: efectos colaterales del SARS-COV2 en la asignatura Fundamentos Médicos del Máster Ingeniería Biomédica. En IN-RED 2021: VII Congreso de Innovación Edicativa y Docencia en Red. Editorial Universitat Politècnica de València. 996-1005. https://doi.org/10.4995/INRED2021.2021.13778OCS996100

El e-learning y el aprendizaje por problemas como metodologías docentes

[EN] The European Higher Education Area, the pandemic and the new competence-based learning model have required changes in teaching and assessment, which has led to the emergence of new teaching methodologies such as e-learning. Within the subject of Nutritional Pathology of the Degree in Human Nutrition and Dietetics, the aim is to implement a change in the methodology from lectures, in which the master class was generally used, to a more participative one. In this project, the "delivery" of the material was moved out of the formal classroom, through the Virtual Classroom platform, and it was used to undertake collaborative activities where students had to solve a series of cases in groups with the help of the material prepared by the teacher (problem-based learning). The results show that the application of the inverted classroom pedagogical model through mobile devices and problem-based learning improves the students' perception of their use of class time, their participation, and their subjective perception of their performance, compared to the traditional pedagogical model.[ES] La entrada en el Espacio Europeo de Educación Superior, la pandemia y el nuevo modelo de aprendizaje por competencias ha requerido cambios en la docencia y en la evaluación, lo que ha propiciado la aparición de nuevas de metodologías de enseñanza como el e-learning. Dentro de la materia de Patología Nutricional del Grado de Nutrición Humana y Dietética, se pretende implantar un cambio de la metodología de las clases expositivas en las que generalmente se empleaba la clase magistral a otro más participativo. En este proyecto se movió la “entrega” del material fuera de la clase formal, a través de la plataforma Aula Virtual, y se hizo uso de ésta para emprender actividades colaborativas donde los estudiantes debían resolver una serie de casos en grupo con la ayuda del material elaborado por la profesora (aprendizaje por problemas). Los resultados ponen de manifiesto que la aplicación del modelo pedagógico de clase invertida a través de dispositivos móviles y aprendizaje por problemas mejora la percepción del alumnado sobre su aprovechamiento del tiempo de clase, su participación, y la percepción subjetiva sobre su rendimiento; respecto al modelo pedagógico tradicional.Innovation Project Universitat de Valencia nº NOU-PID, UV-SFPIE_PID-1641477.Ortiz Masia, MD. (2022). El e-learning y el aprendizaje por problemas como metodologías docentes. Editorial Universitat Politècnica de València. 908-921. https://doi.org/10.4995/INRED2022.2022.1584690892

Immunolocalization of dually phosphorylated MAPKs in dividing root meristem cells of Vicia faba, Pisum sativum, Lupinus luteus and Lycopersicon esculentum

Key message In plants, phosphorylated MAPKs display constitutive nuclear localization; however, not all studied plant species show co-localization of activated MAPKs to mitotic microtubules. Abstract The mitogen-activated protein kinase (MAPK) signaling pathway is involved not only in the cellular response to biotic and abiotic stress but also in the regulation of cell cycle and plant development. The role of MAPKs in the formation of a mitotic spindle has been widely studied and the MAPK signaling pathway was found to be indispensable for the unperturbed course of cell division. Here we show cellular localization of activated MAPKs (dually phosphorylated at their TXY motifs) in both interphase and mitotic root meristem cells of Lupinus luteus, Pisum sativum, Vicia faba (Fabaceae) and Lycopersicon esculentum (Solanaceae). Nuclear localization of activated MAPKs has been found in all species. Colocalization of these kinases to mitotic microtubules was most evident in L. esculentum, while only about 50 % of mitotic cells in the root meristems of P. sativum and V. faba displayed activated MAPKs localized to microtubules during mitosis. Unexpectedly, no evident immunofluorescence signals at spindle microtubules and phragmoplast were noted in L. luteus. Considering immunocytochemical analyses and studies on the impact of FR180204 (an inhibitor of animal ERK1/2) on mitotic cells, we hypothesize that MAPKs may not play prominent role in the regulation of microtubule dynamics in all plant species

Progastrin Represses the Alternative Activation of Human Macrophages and Modulates Their Influence on Colon Cancer Epithelial Cells

Macrophage infiltration is a negative prognostic factor for most cancers but gastrointestinal tumors seem to be an exception. The effect of macrophages on cancer progression depends on their phenotype, which may vary between M1 (pro-inflammatory, defensive) to M2 (tolerogenic, pro-tumoral). Gastrointestinal cancers often become an ectopic source of gastrins and macrophages present receptors for these peptides. The aim of the present study is to analyze whether gastrins can affect the pattern of macrophage infiltration in colorectal tumors. We have evaluated the relationship between gastrin expression and the pattern of macrophage infiltration in samples from colorectal cancer and the influence of these peptides on the phenotype of macrophages differentiated from human peripheral monocytes in vitro. The total number of macrophages (CD68+ cells) was similar in tumoral and normal surrounding tissue, but the number of M2 macrophages (CD206+ cells) was significantly higher in the tumor. However, the number of these tumor-associated M2 macrophages correlated negatively with the immunoreactivity for gastrin peptides in tumor epithelial cells. Macrophages differentiated from human peripheral monocytes in the presence of progastrin showed lower levels of M2-markers (CD206, IL10) with normal amounts of M1-markers (CD86, IL12). Progastrin induced similar effects in mature macrophages treated with IL4 to obtain a M2-phenotype or with LPS plus IFNγ to generate M1-macrophages. Macrophages differentiated in the presence of progastrin presented a reduced expression of Wnt ligands and decreased the number and increased cell death of co-cultured colorectal cancer epithelial cells. Our results suggest that progastrin inhibits the acquisition of a M2-phenotype in human macrophages. This effect exerted on tumor associated macrophages may modulate cancer progression and should be taken into account when analyzing the therapeutic value of gastrin immunoneutralization

Cloning and molecular characterization of a mitogen-activated protein kinase gene from Poncirus trifoliata whose ectopic expression confers dehydration/drought tolerance in transgenic tobacco

The mitogen-activated protein kinase (MAPK) cascade plays pivotal roles in diverse signalling pathways related to plant development and stress responses. In this study, the cloning and functional characterization of a group-I MAPK gene, PtrMAPK, in Poncirus trifoliata (L.) Raf are reported. PtrMAPK contains 11 highly conserved kinase domains and a phosphorylation motif (TEY), and is localized in the nucleus of transformed onion epidermal cells. The PtrMAPK transcript level was increased by dehydration and cold, but was unaffected by salt. Transgenic overexpression of PtrMAPK in tobacco confers dehydration and drought tolerance. The transgenic plants exhibited better water status, less reactive oxygen species (ROS) generation, and higher levels of antioxidant enzyme activity and metabolites than the wild type. Interestingly, the stress tolerance capacity of the transgenic plants was compromised by inhibitors of antioxidant enzymes. In addition, overexpression of PtrMAPK enhanced the expression of ROS-related and stress-responsive genes under normal or drought conditions. Taken together, these data demonstrate that PtrMAPK acts as a positive regulator in dehydration/drought stress responses by either regulating ROS homeostasis through activation of the cellular antioxidant systems or modulating transcriptional levels of a variety of stress-associated genes