A Continuous Lipase-Catalyzed Acylation Process for the Large-Scale Production of Vitamin A Precursors

A continuous enzyme-catalyzed acylation process for the selective preparation of monoacylated Vitamin A precursors starting from a 1,6-diol on a large scale is reported. Screenings led to the selection of the commercially available immobilized lipase Chirazyme L2-C2 (lipase B from Candida antarctica) as the biocatalyst, different vinyl acylates as the acylating agents, and acetone as the co-solvent. Using a mixture of 70% (v/v) acetone and acylating agent allowed to increase the substrate concentration from 10 to 30% (w/w). Using a small fixed-bed reactor, this continuous process produced monoacylated product with >99% yield and >97% selectivity for the primary hydroxy group. The robustness of this system under different conditions was investigated. Consequently, the stability of the biocatalyst could be greatly improved by adding a protective pre-column and by adding small amounts of organic base and antioxidant to the substrate solution. This optimized laboratory process was used to selectively prepare monoacylated compounds in kilogram scales over one hundred days with only a minor decrease in conversion efficiency. The process was also implemented in an up-scaled mini plant for the continuous production on a kilogram-per-day scale, reproducing the results previously obtained on smaller laboratory scales

Mapping and sequencing of an actively transcribedEuglena gracilis chloroplast gene (ccsA) homologous to theArabidopsis thaliana nuclear genecs(ch-42)

AbstractWe mapped and sequenced a novel chloroplast gene encoding a protein (348 amino acids) which shows a high sequence identity with both the decoded nuclearcs(ch-42) gene product ofArabidopsis thaliana, and the C-terminal half of the decoded ‘crtA’ gene product ofRhodobacter capsulatus. The chloroplast gene (ccsA) is split (two exons) and transcribed into a stable mRNA of about 1200 nucleotides. The putative protein may be involved in the biosynthesis of photosynthetic pigments

Évaluation des pratiques de l’injonction de soins : étude sur 119 sujets

International audienceINTRODUCTION: The injunction to care (IC) is a new compulsory treatment created by the Act of June 17, 1998. Initially, this judicial tool concerned mainly sex offenders, but then the number of overall crimes requiring an IC began to dramatically rise. The judge can order this measure only if a psychiatric expertise has concluded to its potential opportunity. Then the convict must undergo a psychiatric follow-up after having served the sentence. The creation of a court-ordered treatment is based on the premise that many offenders are affected by psychiatric disorders. For the lawmaker, the aim is to lower the risk of recidivism.PURPOSES: The main purpose of this study is to assess the convicts concerned by the IC describing their sociodemographic characteristics, psychiatric diagnostics and criminal characteristics. The second purpose is to assess the efficiency of this measure regarding re-offending and specifically legal recidivism.METHODS: This is a retrospective and descriptive study concerning 119 subjects followed-up by two "coordinating doctors" in the department of Sarthe who could assess their psychiatric diagnostics and sociodemographic characteristics. They could also compare medical data with the judicial data for 78 of the subjects.RESULTS: The population was composed of 117 men (98.3%) aged 45 years old on average. They had a job in 37% of cases (n=44) and were single in 56.3% of cases (n=67). According to the DSM-IV-TR, only 29 subjects (24.4%) had an axis I disorder and 37.8% of the population had a pathological personality trait (non DSM-IV-TR categorized disorder). Furthermore, 51.3% (n=61) of the subjects were addicted (mainly alcohol). The medical follow-up was carried out by a psychiatrist in 83.2% of cases (n=99). The average duration of follow-up was five years. Among the 78 subjects for whom there was access to juridical data, 13 (16.7%) had committed a new offense during follow-up. Among them, seven had recidivated six of whom were initially sentenced for sexual offense.CONCLUSIONS: Most of the subjects in injunction to care had no axis I disorder but addictions and/or pathological personality traits. Nevertheless, the expert concluded the need of an IC. Personality and behavior disorders do not always require psychiatric care, and the management must be multidisciplinary. In France, the psychiatrist remains at the center of injunction to care measure. The addictology care is not developed whereas it is a population at risk and there is a lack of interactions between professionals (medical, social and judicial professionals). The IC is a measure that needs to be improved by means of better communication among the different professionals and a better global assessment of the subjects. Medical care must be a possible option but not a systematic treatment.</p