The c4h, tat, hppr and hppd Genes Prompted Engineering of Rosmarinic Acid Biosynthetic Pathway in Salvia miltiorrhiza Hairy Root Cultures

Rational engineering to produce biologically active plant compounds has been greatly impeded by our poor understanding of the regulatory and metabolic pathways underlying the biosynthesis of these compounds. Here we capitalized on our previously described gene-to-metabolite network in order to engineer rosmarinic acid (RA) biosynthesis pathway for the production of beneficial RA and lithospermic acid B (LAB) in Salvia miltiorrhiza hairy root cultures. Results showed their production was greatly elevated by (1) overexpression of single gene, including cinnamic acid 4-hydroxylase (c4h), tyrosine aminotransferase (tat), and 4-hydroxyphenylpyruvate reductase (hppr), (2) overexpression of both tat and hppr, and (3) suppression of 4-hydroxyphenylpyruvate dioxygenase (hppd). Co-expression of tat/hppr produced the most abundant RA (906 mg/liter) and LAB (992 mg/liter), which were 4.3 and 3.2-fold more than in their wild-type (wt) counterparts respectively. And the value of RA concentration was also higher than that reported before, that produced by means of nutrient medium optimization or elicitor treatment. It is the first report of boosting RA and LAB biosynthesis through genetic manipulation, providing an effective approach for their large-scale commercial production by using hairy root culture systems as bioreactors

Population Genetics of GYPB and Association Study between GYPB*S/s Polymorphism and Susceptibility to P. falciparum Infection in the Brazilian Amazon

Merozoites of Plasmodium falciparum invade through several pathways using different RBC receptors. Field isolates appear to use a greater variability of these receptors than laboratory isolates. Brazilian field isolates were shown to mostly utilize glycophorin A-independent invasion pathways via glycophorin B (GPB) and/or other receptors. The Brazilian population exhibits extensive polymorphism in blood group antigens, however, no studies have been done to relate the prevalence of the antigens that function as receptors for P. falciparum and the ability of the parasite to invade. Our study aimed to establish whether variation in the GYPB*S/s alleles influences susceptibility to infection with P. falciparum in the admixed population of Brazil.Two groups of Brazilian Amazonians from Porto Velho were studied: P. falciparum infected individuals (cases); and uninfected individuals who were born and/or have lived in the same endemic region for over ten years, were exposed to infection but have not had malaria over the study period (controls). The GPB Ss phenotype and GYPB*S/s alleles were determined by standard methods. Sixty two Ancestry Informative Markers were genotyped on each individual to estimate admixture and control its potential effect on the association between frequency of GYPB*S and malaria infection.GYPB*S is associated with host susceptibility to infection with P. falciparum; GYPB*S/GYPB*S and GYPB*S/GYPB*s were significantly more prevalent in the in the P. falciparum infected individuals than in the controls (69.87% vs. 49.75%; P<0.02). Moreover, population genetics tests applied on the GYPB exon sequencing data suggest that natural selection shaped the observed pattern of nucleotide diversity.Epidemiological and evolutionary approaches suggest an important role for the GPB receptor in RBC invasion by P. falciparum in Brazilian Amazons. Moreover, an increased susceptibility to infection by this parasite is associated with the GPB S+ variant in this population

Too Many of a Good Thing? The Effects of Multiple Motivations on Stress, Cost, Fulfillment, and Satisfaction

Individuals engage in behaviors to satisfy motivations and can engage in the same behavior to satisfy a variety of different motivations. Previous research has examined how differences in the overall amount of motivation influence outcomes but has not addressed how differences in the number of conceptually distinct motivations one has for a particular action influence outcomes. In two longitudinal field studies, individuals with more than one motivation for volunteering experienced greater negative outcomes than did those who volunteered to satisfy a single motivation. A laboratory study manipulated the number of motivations individuals had for engaging in a volunteer activity. Individuals with two motivations reported greater negative outcomes than did those with one motivation. The importance of these findings for understanding the linkages of motivation and action in self-regulated behaviors is discussed

Using Web 2.0 and Social Media Technologies to Foster Proenvironmental Action

Research from a variety of disciplines suggests that online technologies (i.e., Web 2.0 and social media) have considerable potential for spurring proenvironmental action; however, relatively little work examines how to effectively capitalize on these communication and organization tools. This review paper describes the Technologies for Proenvironmental Action Model (TPAM), a conceptual framework that explicates how different functions of Web 2.0 and social media (i.e., informational, relational, and experiential) can generate and/or facilitate personal, social, and contextual pathways to environmentally responsible behaviors. As derived from the TPAM, the likelihood of achieving practical goals of increasing proenvironmental behaviors is enhanced when technological functions are matched to the different pathways to proenvironmental action. For example, the relational function of technologies, as exemplified by Social Networking Sites (SNSs), should be particularly effective in communicating social norms supportive of environmentally responsible behaviors. The TPAM is intended as a guide to develop novel approaches, research questions, and methodologies in leveraging Web 2.0 and social media technologies to promote proenvironmental action. Results will contribute to basic theory development and work in applied settings (e.g., local environmental organizations) in order to effectively communicate and organize with different segments of the population to increase sustainable behaviors

Effects of cocoa-enriched diet on orofacial pain in a murine model

Objectives: To investigate and discuss the effects of cocoa on orofacial pain. Setting and Sample Population: The Department of Orthodontics at the University of Florida (UF). Male and female hairless rats (N=20/group) were tested. Materials and Methods: Rats were tested using the Orofacial Pain Assessment Device (OPAD) before and after changing their food from the standard chow to a cocoa‐enriched or control‐equivalent diet. Results: Male rats fed the cocoa diet had a significantly higher operant pain index when tested at 37°C as compared to control diet‐fed animals. Female rats on the cocoa diet had a significantly higher pain index when tested at 18°C and 44°C, as compared to animals fed the control diet. Capsaicin‐induced pain was inhibited, with cocoa‐diet male rats having a significantly higher pain index than control‐diet male rats and cocoa‐diet female rats at both 37°C and 44°C. Cocoa‐diet female rats had a significantly higher pain index at 44°C than control‐diet females. Mechanical sensitivity was affected following capsaicin cream, with a significantly decreased tolerated bottle distance in both cocoa‐ and control‐diet animals, but there was no difference between cocoa‐ and control‐diet groups. Conclusion: Using the OPAD operant system, we demonstrated that a diet rich in cocoa was effective in inhibiting neurogenic inflammatory pain in rats. This has implications for the use of novel alternative therapies such as diet modification for pain control

Patterns of genome-wide variation, population differentiation and SNP discovery of the red banded stink bug (piezodorus guildinii)

Unravelling the details of range expansion and ecological dominance shifts of insect pests has been challenging due to the lack of basic knowledge about population structure, gene flow, and most importantly, how natural selection is affecting the adaptive process. Piezodous guildinii is an emerging pest of soybean in the southern region of the United States, and increasingly important in Brazil in recent years. However, the reasons P. guildinii is gradually becoming more of a problem are questions still mostly unanswered. Here, we have genotyped P. guildinii samples and discovered 1,337 loci containing 4,083 variant sites SNPs that were used to estimate genetic structure and to identify gene candidates under natural selection. Our results revealed the existence of a significant genetic structure separating populations according to their broad geographic origin, i.e., U.S. and Brazil, supported by AMOVA (F-GT = 0.26), STRUCTURE, PCA, and F-ST analyses. High levels of gene flow or coancestry within groups (i.e., within countries) can be inferred from the data, and no spatial pattern was apparent at the finer scale in Brazil. Samples from different seasons show more heterogeneous compositions suggesting mixed ancestry and a more complex dynamic. Lastly, we were able to detect and successfully annotated 123 GBS loci (10.5%) under positive selection. The gene ontology (GO) analysis implicated candidate genes under selection with genome reorganization, neuropeptides, and energy mobilization. We discuss how these findings could be related to recent outbreaks and suggest how new efforts directed to better understand P. guildinii population dynamics9CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP202001/2015-62017/02393-0FAPESP (Brazil)Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2017/02393-0]; National Council for Scientific and Technological Development (CNPq)National Council for Scientific and Technological Development (CNPq) [202001/2015-6]; Sao Paulo Research Foundation (FAPESP, Portuguese: Fundacao de Amparo a Pesquisa do Estado de Sao Paulo)Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP); Brazilian National Council for Scientific and Technological Development (CNPq, Portuguese: Conselho Nacional de Desenvolvimento Cientifico e Tecnologico)National Council for Scientific and Technological Development (CNPq); FAPESPFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP