Lycopene Prevents Development of Steatohepatitis in Experimental Nonalcoholic Steatohepatitis Model Induced by High-Fat Diet

We investigated the preventive effect of lycopene on nonalcoholic steatohepatitis-induced by high-fat diet in rats. Forty male Sprague-Dawley rats were divided into 4 groups. They were fed standard diet, high-fat diet (HFD), high-fat diet plus lycopene at a dose of 2 mg/kg body weight and the high-fat diet lycopene at a dose of 4 mg/kg BW for a period of 6 weeks. Inflammation, steatosis, α-smooth muscle actin (α-SMA), and cytochrome P450 2E1 (CYP 2E1) expression increased significantly in the rats fed HFD and decreased in the rats administered by lycopene. Significantly elevated levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), tumor necrosis factor (TNF α), and serum and liver malondialdehyde (MDA) were observed in rats fed the high-fat diet as compared to the control rats (P < .01). Supplementation with lycopene lowered serum MDA and tumor necrosis factor (TNF-α) levels and elevated liver GSH level (P < .001). Insulin resistance was higher in the rats fed HFD than in rats supplemented with lycopene. The data indicate that supplementation with lycopene can reduce high-fat diet-induced oxidative stress to the cells

IRE1α-XBP1s pathway promotes prostate cancer by activating c-MYC signaling

Activation of endoplasmic reticulum (ER) stress/the unfolded protein response (UPR) has been linked to cancer, but the molecular mechanisms are poorly understood and there is a paucity of reagents to translate this for cancer therapy. Here, we report that an IRE1α RNase-specific inhibitor, MKC8866, strongly inhibits prostate cancer (PCa) tumor growth as monotherapy in multiple preclinical models in mice and shows synergistic antitumor effects with current PCa drugs. Interestingly, global transcriptomic analysis reveal that IRE1α-XBP1s pathway activity is required for c-MYC signaling, one of the most highly activated oncogenic pathways in PCa. XBP1s is necessary for optimal c-MYC mRNA and protein expression, establishing, for the first time, a direct link between UPR and oncogene activation. In addition, an XBP1-specific gene expression signature is strongly associated with PCa prognosis. Our data establish IRE1α-XBP1s signaling as a central pathway in PCa and indicate that its targeting may offer novel treatment strategies.</p

Reliability and Validity of the Turkish Version of the Body Image Disturbance Questionnaire-Scoliosis

Study Design.It is cross-cultural adaptation and validation of the Body Image Disturbances Questionnaire.Objective.The purpose of the study was to adopt the English version of the Body Image Disturbance Questionnaire-Scoliosis (BIDQ-S) into Turkish language.Summary of Background Data.BIDQ-S is originally adopted from BIDQ scale which is designed to be used for healthy subjects in order to assess concerns and distress about physical appearance, and impairment on daily functioning. However, there is no culturally adopted and validated BIDQ-S for the Turkish population.Method.Translation and back translation method was used to guide the translation process of the BIDQ-S from English to Turkish. In order to determine and confirm the factor structure of the Turkish BIDQ-S exploratory and confirmatory factor analysis were performed. Convergent validity of the Turkish BIDQ-S- was determined by calculating the correlations of BIDQ-S, and the factors emerged, with the indicators of subjective well-being which consists of three components: positive affect (PA), negative affect (NA), and satisfaction with life (SWL) and of health related quality of life measured by Scoliosis Research Society Questionnaire (SRS-22).Results.The data from the present study demonstrated that different from the original, German and Chinese versions two factor solutions were found. The Turkish BIDQ-S also showed satisfactory internal consistency value with Cronbach a equals to 0.88 construct validity.Conclusion.In conclusion, current study shows that Turkish BIDQ-S is a valid and reliable questionnaire for assessing the body image concerns in patients with scoliosis in Turkish population.Level of Evidence: 4. © 2020 Lippincott Williams and Wilkins. All rights reserved

Current State of Animal (Mouse) Modeling in Melanoma Research

International audienc

Stamp2 expression mediated by cytokines attenuates their growth-limiting effects in prostate cancer cells

Inflammatory events and dysregulated cytokine expression are implicated in prostate cancer (PCa), but the underlying molecular mechanisms are poorly understood at present. We have previously identified six transmembrane protein of the prostate 2 (STAMP2, also known as STEAP4) as an androgen-regulated gene, as well as a key regulator of PCa growth and survival. STAMP2 is also regulated by, and participates in, inflammatory signaling in other tissues and pathologies. Here, we show that the proinflammatory cytokines interleukin 6 (IL-6) and Interleukin 1 beta (IL-1β) significantly increase and strongly synergize in promoting STAMP2 expression in PCa cells. The two cytokines increase androgen-induced STAMP2 expression, but not expression of other known androgen target genes, suggesting a unique interplay of androgens and cytokines in regulating STAMP2 expression. Interestingly, STAMP2 knockdown significantly increased the ability of IL-6 and IL-1β to inhibit PCa cell growth in vitro. These results suggest that STAMP2 may represent a unique node through which inflammatory events mediate their effects on PCa growth and survival

A new mathematical formula to predict the foetal weight in twin pregnancies: A comparison of it with 19 different formulas

One hundred and seventy-two twin-pregnant patients were enrolled. The estimated foetal weight was calculated using 19 different formulas. Ong's formula (0.954 (95%CI=0.938/0.966)), which was designed specifically for twins, produced the highest Cronbach's alpha value followed by Hadlock II (0.952 (95%CI=0.935/0.965)), Hadlock I (0.952 (95%CI=0.935/0.964)), Hadlock III (0.952 (95%CI=0.935/0.964)), Hadlock IV (0.952 (95%CI=0.935/0.964)) and our formula (0.952 (95%CI=0.935/0.964)), which produced the same Cronbach's alpha values for twin A. For twin B, our formula produced the highest Cronbach's alpha value (0.961 (95%CI=0.948/0.972) followed by Hadlock II (0.960 (95%CI=0.946/0.971)), Hadlock I (0.960 (95%CI=0.946/0.970)), Hadlock III (0.960 (95%CI=0.946/0.970)) and Hadlock IV (0.960 (95%CI=0.946/0.970)). In conclusion, our formula (AC, FL) performed well in predicting the foetal weights in twin pregnancies (>24 weeks) in our study. However, it should be tested in other populations. Hadlock II (AC, FL) produced a comparable performance to Hadlock I (BPD, HC, AC, FL), Hadlock III (BPD, AC, FL) and Hadlock IV (HC, AC, FL). Hadlock II may be preferable in twin pregnancies since it is based on AC and FL only