12 research outputs found
Combined point of care nucleic acid and antibody testing for SARS-CoV-2 following emergence of D614G Spike Variant
Rapid COVID-19 diagnosis in hospital is essential, though complicated by 30-50% of nose/throat swabs being negative by SARS-CoV-2 nucleic acid amplification testing (NAAT). Furthermore, the D614G spike mutant now dominates the pandemic and it is unclear how serological tests designed to detect anti-Spike antibodies perform against this variant. We assess the diagnostic accuracy of combined rapid antibody point of care (POC) and nucleic acid assays for suspected COVID-19 disease due to either wild type or the D614G spike mutant SARS-CoV-2. The overall detection rate for COVID-19 is 79.2% (95CI 57.8-92.9%) by rapid NAAT alone. Combined point of care antibody test and rapid NAAT is not impacted by D614G and results in very high sensitivity for COVID-19 diagnosis with very high specificity
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Age-related immune response heterogeneity to SARS-CoV-2 vaccine BNT162b2
Abstract: Although two-dose mRNA vaccination provides excellent protection against SARS-CoV-2, there is little information about vaccine efficacy against variants of concern (VOC) in individuals above eighty years of age1. Here we analysed immune responses following vaccination with the BNT162b2 mRNA vaccine2 in elderly participants and younger healthcare workers. Serum neutralization and levels of binding IgG or IgA after the first vaccine dose were lower in older individuals, with a marked drop in participants over eighty years old. Sera from participants above eighty showed lower neutralization potency against the B.1.1.7 (Alpha), B.1.351 (Beta) and P.1. (Gamma) VOC than against the wild-type virus and were more likely to lack any neutralization against VOC following the first dose. However, following the second dose, neutralization against VOC was detectable regardless of age. The frequency of SARS-CoV-2 spike-specific memory B cells was higher in elderly responders (whose serum showed neutralization activity) than in non-responders after the first dose. Elderly participants showed a clear reduction in somatic hypermutation of class-switched cells. The production of interferon-Îł and interleukin-2 by SARS-CoV-2 spike-specific T cells was lower in older participants, and both cytokines were secreted primarily by CD4 T cells. We conclude that the elderly are a high-risk population and that specific measures to boost vaccine responses in this population are warranted, particularly where variants of concern are circulating
Complement lectin pathway activation is associated with COVID-19 disease severity, independent of MBL2 genotype subgroups
IntroductionWhile complement is a contributor to disease severity in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections, all three complement pathways might be activated by the virus. Lectin pathway activation occurs through different pattern recognition molecules, including mannan binding lectin (MBL), a protein shown to interact with SARS-CoV-2 proteins. However, the exact role of lectin pathway activation and its key pattern recognition molecule MBL in COVID-19 is still not fully understood.MethodsWe therefore investigated activation of the lectin pathway in two independent cohorts of SARS-CoV-2 infected patients, while also analysing MBL protein levels and potential effects of the six major single nucleotide polymorphisms (SNPs) found in the MBL2 gene on COVID-19 severity and outcome.ResultsWe show that the lectin pathway is activated in acute COVID-19, indicated by the correlation between complement activation product levels of the MASP-1/C1-INH complex (p=0.0011) and C4d (p<0.0001) and COVID-19 severity. Despite this, genetic variations in MBL2 are not associated with susceptibility to SARS-CoV-2 infection or disease outcomes such as mortality and the development of Long COVID.ConclusionIn conclusion, activation of the MBL-LP only plays a minor role in COVID-19 pathogenesis, since no clinically meaningful, consistent associations with disease outcomes were noted
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Single-cell multi-omics analysis of the immune response in COVID-19
Funder: Lister Institute of Preventive Medicine; doi: https://doi.org/10.13039/501100001255Funder: University College London, Birkbeck MRC Doctoral Training ProgrammeFunder: The Jikei University School of MedicineFunder: Action Medical Research (GN2779)Funder: NIHR Clinical Lectureship (CL-2017-01-004)Funder: NIHR (ACF-2018-01-004) and the BMA FoundationFunder: Chan Zuckerberg Initiative (grant 2017-174169) and from Wellcome (WT211276/Z/18/Z and Sanger core grant WT206194)Funder: UKRI Innovation/Rutherford Fund Fellowship allocated by the MRC and the UK Regenerative Medicine Platform (MR/5005579/1 to M.Z.N.). M.Z.N. and K.B.M. have been funded by the Rosetrees Trust (M944)Funder: Barbour FoundationFunder: ERC Consolidator and EU MRG-Grammar awardsFunder: Versus Arthritis Cure Challenge Research Grant (21777), and an NIHR Research Professorship (RP-2017-08-ST2-002)Funder: European Molecular Biology Laboratory (EMBL)Abstract: Analysis of human blood immune cells provides insights into the coordinated response to viral infections such as severe acute respiratory syndrome coronavirus 2, which causes coronavirus disease 2019 (COVID-19). We performed single-cell transcriptome, surface proteome and T and B lymphocyte antigen receptor analyses of over 780,000 peripheral blood mononuclear cells from a cross-sectional cohort of 130 patients with varying severities of COVID-19. We identified expansion of nonclassical monocytes expressing complement transcripts (CD16+C1QA/B/C+) that sequester platelets and were predicted to replenish the alveolar macrophage pool in COVID-19. Early, uncommitted CD34+ hematopoietic stem/progenitor cells were primed toward megakaryopoiesis, accompanied by expanded megakaryocyte-committed progenitors and increased platelet activation. Clonally expanded CD8+ T cells and an increased ratio of CD8+ effector T cells to effector memory T cells characterized severe disease, while circulating follicular helper T cells accompanied mild disease. We observed a relative loss of IgA2 in symptomatic disease despite an overall expansion of plasmablasts and plasma cells. Our study highlights the coordinated immune response that contributes to COVID-19 pathogenesis and reveals discrete cellular components that can be targeted for therapy
SARS-CoV-2 B.1.617.2 Delta variant replication and immune evasion
Abstract: The B.1.617.2 (Delta) variant of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first identified in the state of Maharashtra in late 2020 and spread throughout India, outcompeting pre-existing lineages including B.1.617.1 (Kappa) and B.1.1.7 (Alpha)1. In vitro, B.1.617.2 is sixfold less sensitive to serum neutralizing antibodies from recovered individuals, and eightfold less sensitive to vaccine-elicited antibodies, compared with wild-type Wuhan-1 bearing D614G. Serum neutralizing titres against B.1.617.2 were lower in ChAdOx1 vaccinees than in BNT162b2 vaccinees. B.1.617.2 spike pseudotyped viruses exhibited compromised sensitivity to monoclonal antibodies to the receptor-binding domain and the amino-terminal domain. B.1.617.2 demonstrated higher replication efficiency than B.1.1.7 in both airway organoid and human airway epithelial systems, associated with B.1.617.2 spike being in a predominantly cleaved state compared with B.1.1.7 spike. The B.1.617.2 spike protein was able to mediate highly efficient syncytium formation that was less sensitive to inhibition by neutralizing antibody, compared with that of wild-type spike. We also observed that B.1.617.2 had higher replication and spike-mediated entry than B.1.617.1, potentially explaining the B.1.617.2 dominance. In an analysis of more than 130 SARS-CoV-2-infected health care workers across three centres in India during a period of mixed lineage circulation, we observed reduced ChAdOx1 vaccine effectiveness against B.1.617.2 relative to non-B.1.617.2, with the caveat of possible residual confounding. Compromised vaccine efficacy against the highly fit and immune-evasive B.1.617.2 Delta variant warrants continued infection control measures in the post-vaccination era
Identification et caractĂ©risation fonctionnelle de LACC1 dans les formes familiales de lâArthrite JuvĂ©nile Idiopathique
Les maladies inflammatoires Ă dĂ©but pĂ©diatrique sont des situations rares de dĂ©rĂ©gulation du systĂšme immunitaire dont les causes sont peu connues et supposĂ©es multifactorielles. Lâarthrite juvĂ©nile idiopathique (AJI) est responsable dâune inflammation articulaire chronique chez lâenfant dont les causes restent indĂ©finies. Les formes familiales de cette maladie suggĂšrent une cause gĂ©nĂ©tique et les nouvelles techniques de sĂ©quençage haut dĂ©bit nous permettent dâidentifier des variants rares prĂ©dits pathogĂ©niques. Nous avons identifiĂ© 3 familles dâAJI dont les patients sont porteurs de mutations homozygotes exoniques dans le gĂšne LACC1. Il existe dans la littĂ©rature des donnĂ©es contradictoires sur LACC1 et son implication dans les maladies inflammatoires. Afin de mieux comprendre son rĂŽle, nous avons dĂ©veloppĂ© plusieurs outils translationels afin dâĂ©tudier LACC1 Ă la fois chez lâhomme et la souris. A lâaide de cellules primaires humaines, nous avons mis en Ă©vidence que lâexpression de LACC1 Ă©tait induite par le M-CSF via la voie mTOR au cours de la diffĂ©rentiation des monocytes en macrophages spĂ©cifiquement. Chez les patients, les mutations dans le gĂšne LACC1 conduisent Ă une perte dâexpression de la protĂ©ine dans les macrophages indiquant quâune perte de fonction dans LACC1 est impliquĂ©e dans le dĂ©veloppement de lâAJI. De plus, pour la premiĂšre fois, nous avons observĂ© que LACC1 promouvait le flux autophagique ainsi que le niveau de corps lipidique qui alimente la respiration mitochondriale. De plus, nous avons montrĂ© que le liquide synovial, composant majeur des articulations, rĂ©duit le flux autophagique et la respiration mitochondriale. Ce microenvironnement potentialise le caractĂšre inflammatoire des macrophages dĂ©ficients en LACC1. Ces rĂ©sultats suggĂšrent que lâĂ©quilibre du flux autophagique est important dans un contexte de dĂ©ficience en LACC1 oĂč le microenvironnement favorise lâinflammation par les macrophages. De ce fait, les thĂ©rapies visant Ă moduler lâautophagie peuvent reprĂ©senter un atout dans le traitement des maladies causĂ©es par une dĂ©ficience en LACC1.Pediatric-onset inflammatory diseases arise from deregulated, uncontrolled and exacerbated immune responses. Juvenile Idiopathic Arthritis (JIA) is one of the most frequent and is associated to chronic inflammation of the joints. The causes of JIA are multifactorial and poorly understood. Familial and very early-onset cases are rare and suggest genetic inheritance of the disease. Next generation sequencing combined with advanced bioinformatics enable us to identify rare variants predicted to be highly pathogenic. We identified 3 families with JIA patients carrying homozygous mutations in the gene encoding for LACC1. In the literature, there exist conflicting data on LACC1 and its role in inflammatory diseases. In order to dissect the role of LACC1, we developed several translational tolls based on both human and mice models. Using primary human cells, we showed that the specific expression of LACC1 is stimulated by M-CSF via the mTOR pathway during monocyte-macrophage differentiation. In patientâs macrophages, mutations in LACC1 gene led to loss of expression in macrophages implying the loss of function of LACC1 contributes to the development of JIA. In addition, we demonstrated for the first time that LACC1 promotes autophagy flux and impact on lipid droplet levels which fed mitochondrial respiration. Furthermore, we showed that synovial fluid, a major component of joints, reduced autophagy flux of LACC1-defieinct macrophages and mitochondrial respiration. Moreover, this microenviroment potentializes the inflammatory character of LACC1-deficient macrophages. These evidences suggest that autophagy homeostasis is crucial in the context of LACC1 deficiency in which the synovial microenvironment favors inflammation mediated by macrophages. Autophagy-modulating drugs could represent a potential therapeutic option in LACC1 deficiency
RĂŽle des macrophages dans le dĂ©veloppement de lâarthrite juvĂ©nile: LACC1 fait-il le lien ?
International audienceNo abstract availabl
Monogenic lupus: Dissecting heterogeneity
Systemic lupus erythematosus (SLE) is a severe lifelong multisystem autoimmune disease characterized by the presence of autoantibodies targeting nuclear autoantigens, increased production of type I interferon and B cell abnormalities. Clinical presentation of SLE is extremely heterogeneous and different groups of disease are likely to exist. Recently, childhood-onset SLE (cSLE) cases have been linked to single gene mutations, defining the concept of monogenic or Mendelian lupus. Genes associated with Mendelian lupus can be grouped in at least three functional categories. First, complement deficiencies represent the main cause of monogenic lupus and its components are involved in the clearance of dying cells, a mechanism also called efferocytosis. Mutations in extracellular DNASE have been also identified in cSLE patients and represent additional causes leading to defective clearance of nucleic acids and apoptotic bodies. Second, the study of Aicardi-GoutiĂšres syndromes has introduced the concept of type-I interferonopathies. Bona fide lupus syndromes have been associated to this genetic condition, driven by defective nucleic acids metabolism or innate sensors overactivity. Interferon signalling anomalies can be detected and monitored during therapies, such as Janus-kinase (JAK) inhibitors. Third, tolerance breakdown can occur following genetic mutations in B and/or T cell expressing key immunoregulatory molecules. Biallelic mutations in PRKCD are associated to lupus and lymphoproliferative diseases as PKC-ÎŽ displays proapoptotic activity and is crucial to eliminate self-reactive transitional B cells. Here we review the literature of the emerging field of Mendelian lupus and discuss the physiopathological learning from these inborn errors of immunity. In addition, clinical and biological features are highlighted as well as specific therapies that have been tested in these genetic contexts
LACC1 deficiency links juvenile arthritis with autophagy and metabolism in macrophages
International audienceJuvenile idiopathic arthritis is the most common chronic rheumatic disease in children, and its etiology remains poorly understood. Here, we explored four families with early-onset arthritis carrying homozygous loss-of-expression mutations in LACC1. To understand the link between LACC1 and inflammation, we performed a functional study of LACC1 in human immune cells. We showed that LACC1 was primarily expressed in macrophages upon mTOR signaling. We found that LACC1 deficiency had no obvious impact on inflammasome activation, type I interferon response, or NF-ÎșB regulation. Using bimolecular fluorescence complementation and biochemical assays, we showed that autophagy-inducing proteins, RACK1 and AMPK, interacted with LACC1. Autophagy blockade in macrophages was associated with LACC1 cleavage and degradation. Moreover, LACC1 deficiency reduced autophagy flux in primary macrophages. This was associated with a defect in the accumulation of lipid droplets and mitochondrial respiration, suggesting that LACC1-dependent autophagy fuels macrophage bioenergetics metabolism. Altogether, LACC1 deficiency defines a novel form of genetically inherited juvenile arthritis associated with impaired autophagy in macrophages