Responses in bacterial community structure to waste nutrients from aquaculture: an in situ microcosm experiment in a Chilean fjord

Indexación: Web of Science; Scopus.Chilean salmon farms release inorganic nutrients excreted by the fish into the surrounding water in Patagonian fjords. The objective of this experiment from the Comau Fjord (42.2 degrees S) in southern Chile was to study how increased input of ammonium (NH4) and phosphate (PO4) from salmon farms might affect the community structure of bacteria in surface waters where fish farms are located. We used microcosms (35 l) with NH4-N and PO4-P added to the natural seawater in a gradient of nutrient-loading rates, with the same N: P ratio as in salmon aquaculture effluents. Additionally, we measured bacterial community structure at different depths in the Comau Fjord to assess the natural variation to compare with our experiment. We used denaturing gradient gel electrophoresis (DGGE) to create 16S rDNA fingerprints of the bacterial communities and monitored biological and environmental variables (chlorophyll a, inorganic nutrients, pH, microbial abundance). The nutrient- loading rate had a significant impact on the bacterial community structure, and the community dissimilarity between low and high nutrient additions was up to 78%. Of the measured environmental variables, phytoplankton abundance and increased pH from photosynthesis had a significant effect. We observed no significant changes in bacterial diversity, which remained at the same level as in the unmanipulated community. Thus, the bacterial community of the fjord was not resistant, but resilient within the time frame and nutrient gradient of our experiment.http://www.int-res.com/abstracts/aei/v9/p21-32

Neutron skin uncertainties of Skyrme energy density functionals

Background: Neutron-skin thickness is an excellent indicator of isovector properties of atomic nuclei. As such, it correlates strongly with observables in finite nuclei that depend on neutron-to-proton imbalance and the nuclear symmetry energy that characterizes the equation of state of neutron-rich matter. A rich worldwide experimental program involving studies with rare isotopes, parity violating electron scattering, and astronomical observations is devoted to pinning down the isovector sector of nuclear models. Purpose: We assess the theoretical systematic and statistical uncertainties of neutron-skin thickness and relate them to the equation of state of nuclear matter, and in particular to nuclear symmetry energy parameters. Methods: We use the nuclear superfluid Density Functional Theory with several Skyrme energy density functionals and density dependent pairing. To evaluate statistical errors and their budget, we employ the statistical covariance technique. Results: We find that the errors on neutron skin increase with neutron excess. Statistical errors due to uncertain coupling constants of the density functional are found to be larger than systematic errors, the latter not exceeding 0.06 fm in most neutron-rich nuclei across the nuclear landscape. The single major source of uncertainty is the poorly determined slope L of the symmetry energy that parametrizes its density dependence. Conclusions: To provide essential constraints on the symmetry energy of the nuclear energy density functional, next-generation measurements of neutron skins are required to deliver precision better than 0.06 fm.Comment: 5 pages, 4 figure

vbyCaHbeta CCD Photometry of Clusters. VIII. The Super-Metal Rich, Old Open Cluster NGC 6791

CCD photometry on the intermediate-band vbyCaHbeta system is presented for the metal-rich, old open cluster, NGC 6791. Preliminary analysis led to [Fe/H] above +0.4 with an anomalously high reddening and an age below 5 Gyr. A revised calibration between (b-y)_0 and [Fe/H] at a given temperature shows that the traditional color-metallicity relations underestimate the color of the turnoff stars at high metallicity. With the revised relation, the metallicity from hk and the reddening for NGC 6791 become [Fe/H] = +0.45 +/- 0.04 and E(b-y) = 0.113 +/- 0.012 or E(B-V) = 0.155 +/- 0.016. Using the same technique, reanalysis of the photometry for NGC 6253 produces [Fe/H] = +0.58 +/-0.04 and E(b-y) = 0.120 +/- 0.018 or E(B-V) = 0.160 +/- 0.025. The errors quoted include both the internal and external errors. For NGC 6791, the metallicity from m_1 is a factor of two below that from hk, a result that may be coupled to the consistently low metal abundance from DDO photometry of the cluster and the C-deficiency found from high dispersion spectroscopy. E(B-V) is the same value predicted from Galactic reddening maps. With E(B-V) = 0.15 and [Fe/H] = +0.45, the available isochrones predict an age of 7.0 +/- 1.0 Gyr and an apparent modulus of (m-M) = 13.60 +/- 0.15, with the dominant source of the uncertainty arising from inconsistencies among the isochrones. The reanalysis of NGC 6253 with the revised lower reddening confirms that on both the hk and m_1 metallicity scales, NGC 6253, while less than half the age of NGC 6791, remains at least as metal-rich as NGC 6791, if not richer.Comment: Accepted for Astronomical Journal. 42 p. latex file includes 11 figures and 3 tables, one of which is a short version of a data table to appear in online AJ in its entiret

A Temporal Analysis of Water Quality Variability at the Seattle Aquarium in Elliott Bay, Puget Sound, WA

The Seattle Aquarium is centrally located on Elliott Bay in Puget Sound, built on a pier along the central waterfront in Seattle, WA, USA. The Seattle Aquarium Water Quality Laboratory regularly measures water quality metrics on incoming saltwater pumped directly from Elliott Bay for use in the animal exhibits. This study provides a descriptive temporal analysis of variability in the incoming saltwater conducted from 2007 through 2016. Parameters measured on a weekly basis include ammonia (NH3), nitrite (NO2), pH and fecal coliform bacteria. Ammonia mean throughout the dataset was 0.02 mg/l (SE ± 0.0005), with clear seasonal trends of higher ammonia levels during the summer months (May, June and July) annually. Nitrite mean was 0.01 mg/l (SE ± 0.002), with clear seasonal trends of this nutrient with bi-annual peaks in spring and fall (May and September). Saltwater pH mean was 7.81 (SE ± 0.004), trending lower in winter and spring and higher in summer and fall. Fecal coliform bacteria mean over the 10-year period was 20 colony-forming units (CFU) per 100 ml of water. Overall, Elliott Bay water quality remained relatively stable from 2007 to 2016, and if remains unchanged, will continue to be a reliable source of saltwater with known water quality parameters for use in animal exhibits in the Seattle Aquarium

SU(3) Predictions of B→PPB\to PP Decays in the Standard Model

With SU(3) symmetry one only needs 13 hadronic parameters to describe $B\to PP$ decays in the Standard Model. When annihilation contributions are neglected, only 7 hadronic parameters are needed. These parameters can be determined from existing experimental data and some unmeasured branching ratios and CP asymmetries of the type $B\to PP$ can be predicted. In this talk we present SU(3) predictions of branching ratios and CP asymmetries for $B\to PP$ decays in the Standard Model.Comment: 4 pages, no figure. Talk present at the 5th International Conference on Hyperons, Charm and Beauty Hadrons, Vancouver, June 200

Observations of snowpack properties to evaluate ground-based microwave remote sensing

Active microwave radar has been shown to have great potential for estimating snow water equivalent (SWE) globally from space. To help evaluate optimal active microwave sensor configurations to observe SWE, we evaluated ground-based Frequency Modulated Continuous Wave (FMCW) radar (12–18 GHz, cross-polarisation) using very high resolution in-situ observations of snowpack layering, dielectric permittivity and density over a 10 m snow trench on Toolik Lake, Alaska. Results showed that the thicknesses of layers within the 10 m trench were highly variable over short distances (< 1 m), even where total snow depth changed very little. Layer boundaries observed using NIR photography identified all bands of high radar backscatter. Although additional observations of density and dielectric permittivity helped to explain the causes of backscatter, not all snowpack properties which cause backscatter were coincident with strong vertical changes in density or permittivity. Further observations of high surface roughness in layer boundaries explained some areas of weak backscatter, nonetheless it was shown that a suite of coincident observations, rather than a single technique in isolation, were required to adequately explain the variability of backscatter and the influence of snowpack properties upon it

A Dramatic Decrease in Carbon Star Formation in M31

We analyze resolved stellar near-infrared photometry of 21 HST fields in M31 to constrain the impact of metallicity on the formation of carbon stars. Observations of nearby galaxies show that the carbon stars are increasingly rare at higher metallicity. Models indicate that carbon star formation efficiency drops due to the decrease in dredge-up efficiency in metal-rich thermally-pulsing Asymptotic Giant Branch (TP-AGB) stars, coupled to a higher initial abundance of oxygen. However, while models predict a metallicity ceiling above which carbon stars cannot form, previous observations have not yet pinpointed this limit. Our new observations reliably separate carbon stars from M-type TP-AGB stars across 2.6-13.7 kpc of M31's metal-rich disk using HST WFC3/IR medium-band filters. We find that the ratio of C to M stars (C/M) decreases more rapidly than extrapolations of observations in more metal-poor galaxies, resulting in a C/M that is too low by more than a factor of 10 in the innermost fields and indicating a dramatic decline in C star formation efficiency at metallicities higher than [M/H] $\approx$ -0.1 dex. The metallicity ceiling remains undetected, but must occur at metallicities higher than what is measured in M31's inner disk ([M/H] $\gtrsim$ +0.06 dex).Comment: 16 pages, 13 Figures; text clarifications in response to the referee. Results are unchanged; accepted for publication in Ap