Nonlinear Zeeman Effects in the Cavity-Enhanced Emission of Polarised Photons

We theoretically and experimentally investigate nonlinear Zeeman effects within a polarised single-photon source that uses a single 87Rb atom strongly coupled to a high finesse optical cavity. The breakdown of the atomic hyperfine structure in the D2 transition manifold for intermediate strength magnetic fields is shown to result in asymmetric and, ultimately, inhibited operation of the polarised atom-photon interface. The coherence of the system is considered using Hong-Ou-Mandel interference of the emitted photons. This informs the next steps to be taken and the modelling of future implementations, based on feasible cavity designs operated in regimes minimising nonlinear Zeeman effects, is presented and shown to provide improved performance.Comment: 12 pages, 8 figure

Polarisation oscillations in birefringent emitter-cavity systems

We present the effects of resonator birefringence on the cavity-enhanced interfacing of quantum states of light and matter, including the first observation of single photons with a time-dependent polarisation state that evolves within their coherence time. A theoretical model is introduced and experimentally verified by the modified polarisation of temporally-long single photons emitted from a $^{87}$Rb atom coupled to a high-finesse optical cavity by a vacuum-stimulated Raman adiabatic passage (V-STIRAP) process. Further theoretical investigation shows how a change in cavity birefringence can both impact the atom-cavity coupling and engender starkly different polarisation behaviour in the emitted photons. With polarisation a key resource for encoding quantum states of light and modern micron-scale cavities particularly prone to birefringence, the consideration of these effects is vital to the faithful realisation of efficient and coherent emitter-photon interfaces for distributed quantum networking and communications.Comment: 9 pages, 5 figures including Supplemental Materia

Gene dynamics of toll-like receptor 4 through a population bottleneck in an insular population of water voles (Arvicola amphibius)

Acknowledgments We would like to thank all colleagues who have contributed to fieldwork and sampling during this study. We would especially like to thank Marius Wenzel and Sandra Telfer for collaboration with different aspects of the study, and Dave Jones and Nat Jones for Bartonella PCR assays. This work was supported by the BBSRC studentship to MKG (BB/J01446X/1) and a NERC studentship to MKO. The research was carried out under project license PPL 40/1813.Peer reviewedPublisher PD

Density-and trait-mediated effects of a parasite and a predator in a tri-trophic food web

1. Despite growing interest in ecological consequences of parasitism in food webs, relatively little is known about effects of parasites on long-term population dynamics of non-host species or about whether such effects are density- or trait- mediated. 2. We studied a tri-trophic food chain comprised of: (i) a bacterial basal resource (Serratia fonticola), (ii) an intermediate consumer (Paramecium caudatum), (iii) a top predator (Didinium nasutum), and (iv) a parasite of the intermediate consumer (Holospora undulata). A fully-factorial experimental manipulation of predator and parasite presence/absence was combined with analyses of population dynamics, modelling, and analyses of host (Paramecium) morphology and behavior. 3. Predation and parasitism each reduced the abundance of the intermediate consumer (Paramecium), and parasitism indirectly reduced the abundance of the basal resource (Serratia). However, in combination, predation and parasitism had non-additive effects on the abundance of the intermediate consumer, as well as on that of the basal resource. In both cases, the negative effect of parasitism seemed to be effaced by predation. 4. Infection of the intermediate consumer reduced predator abundance. Modelling and additional experimentation revealed that this was most likely due to parasite reduction of intermediate host abundance (a density-mediated effect), as opposed to changes in predator functional or numerical response. 5. Parasitism altered morphological and behavioural traits, by reducing host cell length and increasing the swimming speed of cells with moderate parasite loads. Additional tests showed no significant difference in Didinium feeding rate on infected and uninfected hosts, suggesting that the combination of these modifications does not affect host vulnerability to predation. However, estimated rates of encounter with Serratia based on these modifications were higher for infected Paramecium than for uninfected Paramecium. 6. A mixture of density-mediated and trait-mediated indirect effects of parasitism on non- host species creates rich and complex possibilities for effects of parasites in food webs that should be included in assessments of possible impacts of parasite eradication or introduction

The XRCC1 phosphate-binding pocket binds poly (ADP-ribose) and is required for XRCC1 function

Poly (ADP-ribose) is synthesized at DNA single-strand breaks and can promote the recruitment of the scaffold protein, XRCC1. However, the mechanism and importance of this process has been challenged. To address this issue, we have characterized the mechanism of poly (ADP-ribose) binding by XRCC1 and examined its importance for XRCC1 function. We show that the phosphate-binding pocket in the central BRCT1 domain of XRCC1 is required for selective binding to poly (ADP-ribose) at low levels of ADP-ribosylation, and promotes interaction with cellular PARP1. We also show that the phosphate-binding pocket is required for EGFP-XRCC1 accumulation at DNA damage induced by UVA laser, H2O2, and at sites of sub-nuclear PCNA foci, suggesting that poly (ADP-ribose) promotes XRCC1 recruitment both at single-strand breaks globally across the genome and at sites of DNA replication stress. Finally, we show that the phosphate-binding pocket is required following DNA damage for XRCC1-dependent acceleration of DNA single-strand break repair, DNA base excision repair, and cell survival. These data support the hypothesis that poly (ADP-ribose) synthesis promotes XRCC1 recruitment at DNA damage sites and is important for XRCC1 function

Environmental hazards and society: Landsliding in Basilicata, Italy, with specific reference to Grassano.

This dissertation takes a realist approach to examine landsliding in the Basilicata region of Italy, with specific reference to the municipality of Grassano, in order to understand humankind's role in contributing to environmental hazards. It concludes that environmental hazards such as landslides have partly-social causes, which are characteristic of the societies they affect, and any real accommodation with environmental hazards must involve radical social change. The dissertation analyzes the differing explanations for environmental hazards given by previous schools of thought. Passing to the empirical material to be examined using these ideas, it describes the current pattern of landslides in Basilicata and discusses whether the reported landslide hazard has increased during the twentieth century. It then examines the physical environment of the region, and discusses the extent to which recent reported changes in the intensity of landslides are correlated to changes in the use of the land. The example of the municipality of Grassano allows an examination of the causative mechanisms behind the regional trends. The dissertation examines the vulnerability of Grassano's physical environment to landslides, and the social factors which have influenced landsliding there. It then discusses the history of landslides in the municipality, and the extent to which they have intensified during the twentieth century. Finally it questions the degree to which reported changes in the level of landslides in Grassano have been the result of a growing sensitivity to landslides. The dissertation's conclusion summarizes the work done, then evaluates the contribution to academic knowledge. It ends with an assessment of the viability of likely future approaches to reducing the landslide hazard in Basilicata

Dietary Protein for Training Adaptation and Body Composition Manipulation in Track and Field Athletes

Track and field athletes engage in vigorous training that places stress on physiological systems requiring nutritional support for optimal recovery. Of paramount importance when optimising recovery nutrition are rehydration and refuelling which are covered in other papers in this volume. Here, we highlight the benefits for dietary protein intake over and above requirements set out in various countries at ~0.8-1.0 g/kg body mass (BM)/day for training adaptation, manipulating body composition, and optimising performance in track and field athletes. To facilitate the remodelling of protein-containing structures, which are turning over rapidly due to their training volumes, track and field athletes with the goal of weight maintenance or weight gain should aim for protein intakes of ~1.6 g/kg BM/day. Protein intakes at this level would not necessarily require an over-emphasis on protein-containing foods and, beyond convenience, does not suggest a need to use protein or amino acid-based supplements. This review also highlights that optimal protein intakes may exceed 1.6 g/kg BM/day for athletes who are restricting energy intake and attempting to minimise loss of lean body mass. We discuss the underpinning rationale for weight loss in track and field athletes, explaining changes in metabolic pathways that occur in response to energy restriction when manipulating protein intake and training. Finally, this review offers practical advice on protein intakes that warrant consideration in allowing an optimal adaptive response for track and field athletes seeking to train effectively and to lose fat mass while energy restricted with minimal (or no) loss of lean body mass

Long-chain n-3 fatty acids as an essential link between musculoskeletal and cardio-metabolic health in older adults

This narrative review aims to critically evaluate scientific evidence exploring the therapeutic role(s) of long-chain n-3 PUFA in the context of ageing, and specifically, sarcopenia. We highlight that beyond impairments in physical function and a lack of independence, the age-related decline in muscle mass has ramifications for cardio-metabolic health. Specifically, skeletal muscle is crucial in regulating blood glucose homeostasis (and by extension reducing type 2 diabetes mellitus risk) and providing gluconeogenic precursors that are critical for survival during muscle wasting conditions (i.e. AIDS). Recent interest in the potential anabolic action of n-3 PUFA is based on findings from experimental studies that measured acute changes in the stimulation of muscle protein synthesis (MPS) and/or chronic changes in muscle mass and strength in response to fish oil-derived n-3 PUFA supplementation. Key findings include a potentiated response of MPS to amino acid provision or resistance-based exercise with n-3 PUFA in healthy older adults that extrapolated to longer-term changes in muscle mass and strength. The key mechanism(s) underpinning this enhanced response of MPS remains to be fully elucidated, but is likely driven by the incorporation of exogenous n-3 PUFA into the muscle phospholipid membrane and subsequent up-regulation of cell signalling proteins known to control MPS. In conclusion, multiple lines of evidence suggest that dietary n-3 PUFA provide an essential link between musculoskeletal and cardio-metabolic health in older adults. Given that western diets are typically meagre in n-3 PUFA content, nutritional recommendations for maintaining muscle health with advancing age should place greater emphasis on dietary n-3 PUFA intake