As iniciativas de economia solidária presentes em Santa Catarina: análise da microrregião do Alto Vale do Itajaí

A economia solidária está presente em diversos empreendimentos no Alto Vale do Itajaí, em forma de associações e cooperativas, que buscam uma alternativa viável de colocação no mercado. Dentre os 28 municípios que compõem a região, 34 empreendimentos de economia solidária estão cadastrados na base de dados do Atlas Digital da Economia Solidária, distribuídos em 17 municípios. Diante desse cenário o presente artigo tem o objetivo de analisar as iniciativas econômicas que se enquadram no sistema de economia solidária, presentes na região do Alto Vale do Itajaí - SC, no período recente, bem como explorar qual o perfil dos participantes. Para realização da pesquisa utilizou-se a metodologia de pesquisa bibliográfica, para elaboração do referencial teórico e para obtenção de dados utilizou-se as informações do atlas Digital da Economia solidária. As principais conclusões do estudo foram que a economia solidária ainda é pouco difundida na região do Alto Vale do Itajaí, devido a isso torna-se pouco explorada, seja por parte da população, grupos sociais ou órgãos públicos o que dificulta o trabalho de divulgação e orientação, inibindo o desejo de empreender de forma alternativa. Muitos empreendimentos de economia solidária surgem da necessidade dos próprios sócios de obterem renda para subsistência ou complementação da pouca já existente ou ainda mediante alguma dificuldade enfrentada no cotidiano

Cytotoxicity and Effects of a New Calcium Hydroxide Nanoparticle Material on Production of Reactive Oxygen Species by LPS-Stimulated Dental Pulp Cells

Introduction: The aim of this study was to synthesize and characterize calcium hydroxide (CH) nanoparticles [CH-NP] and compare the cytotoxicity of these materials with that of mineral trioxide aggregate (White MTA) in human dental pulp mesenchymal cells (hDPMCs) stimulated by lipopolysaccharide (LPS). Methods and Materials: The CH-NP were synthesized by the co-precipitation method, and the physical properties were investigated through X-ray diffraction, scanning electron microscopy (SEM) and energy dispersive x-ray spectrometry (EDS). LPS-stimulated hDPMCs were placed in contact with different dilutions of culture media previously exposed to CH-NP and white MTA for 24 h. The groups were tested for cell viability by MTT formazan and Alamar Blue assays, the production of nitric oxide (NO) by Griess method and the production of reactive oxygen species (ROS) by means of the fluorescent oxidant-sensing probe 2’,7’-dichlorodihydrofluorescein diacetate (DCFH-DA). Control groups for viability test were maintained in DMEM (not LPS-stimulated). For NO and ROS production, negative control group was cells in DMEM, and positive control was cells stimulated by LPS. The results were statistically analyzed by two-way ANOVA, Tukey’s test and Dunnett’s test (ɑ=0.05). Results: The results showed that the cell viability remained above 50% in all materials, independent of the dilution in MTT formazan and Alamar Blue tests. MTA showed a reduction in NO production at dilutions of 1:4 to 1:32 compared with the positive control group (P<0.05). The tested materials exhibited lower ROS production by DPMCs than that by cells in the positive control group (P<0.05), and similar ROS production to the negative control group (P>0.05). Conclusion: The outcomes of present in vitro study showed that MTA and [CH-NP] were not cytotoxic materials, with MTA closer to the results of control group (DMEM). MTA and [CH-NP] reduced ROS production at basal levels, with MTA inhibiting NO production at higher dilutions

Extraction of bioactive compounds from Curcuma longa L. using deep eutectic solvents: in vitro and in vivo biological activities

In this work, deep eutectic solvents (DES-based menthol and cholinium chloride) and the ethanol, temperature, and times were selected to extract bioactive compounds from the rhizome, leaves, and flowers Curcuma longa L., using ultrasound-assisted extraction. Analyzes antioxidant, flavonoids, antimicrobial, chelation Fe2+, inhibition of the cholinesterase's enzymes, cytotoxicity, and genotoxicity in Allium cepa cells were performed. The extracts showed results of iron chelation and antibacterial. Curcuma flowers and leaves' extracts inhibited food spoilage bacteria with values above 45%, with substantial iron-chelating activity above 50%. Extracts obtained by DES based on menthol and lactic acid exhibited a high percentage of inhibition of acetyl and butyryl cholinesterase. In contrast, flower extracts obtained by menthol and acetic acid showed low inhibition of cholinesterase enzyme activity. No extract showed cytotoxicity and genotoxicity. Biological activities showed a high potential for the application of these extracts in the food and pharmaceutical industries.This study was financed by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) – Finance Code 001. The authors thank both CAPES for the financial support, the Federal University of Paraná (UFPR) and the Federal University of Technology - Paraná (UTFPR), Labmulti-CM (UTFPR) for the technical support provided. M. R. Mafra and L. Igarashi-Mafra are grateful to the Brazilian National Council for Scientific and Technological Development (CNPq - Grant 310182/2018-2 and 308517/2018-0, respectively).info:eu-repo/semantics/publishedVersio

Antibacterial efficacy of Enterococcus microencapsulated bacteriocin on Listeria monocytogenes, Listeria innocua and Listeria ivanovi

This study focused on the microencapsulation of enterocin from Enterococcus durans (E. durans MF5) in whey powder (WP) using a spray-drying technique followed by the evaluation of how complexation can preserve the enterocin structure and antimicrobial activity against foodborne pathogens. Crude enterocin samples (1 and 5%) were microencapsulated in 10% WP. The antimicrobial activity of unencapsulated (crude) enterocin and microencapsulated enterocin was tested against the target bacteria Salmonella Typhimurium, Escherichia coli, Listeria monocytogenes, Listeria innocua, and Listeria ivanovi. The microencapsulation yields were 31.66% and 34.16% for concentrations of 1 and 5% enterocin, respectively. There was no significant difference between these concentrations. Microencapsulated enterocin was efficient for up to 12 h of cocultivation with Listeria sp., and the concentration required to inhibit the growth of target bacteria presented values of 6400 AU/ mL (arbitrary unit). Microencapsulated enterocin demonstrated enhanced efficacy against Listeria species and E. coli when compared with crude enterocin (p < 0.05). Fourier transform-infrared spectroscopy and differential scanning calorimetry results confirmed the presence of enterocin in the microparticles. Scanning electron microscopy showed cell damage of the target bacteria. The results showed that complexation with WP preserved enterocin antimicrobial activity during spray-drying, indicating its potential use as a food preservative.This work was supported by Fundação Araucária/Governo do Paraná—Brazil, UTFPR-Londrina Campus and State University of Londrina-Paraná. The authors thank the “Central Analítica Multiusuário da UTFPR Campo Mourão (CAMulti-CM)¨ and “Laboratório Multiusuário da UTFPR Campus Londrina (LabMulti-LD)¨ for the analyses. Fernanda V. Leimann (process 039/2019) thanks to Fundação Araucária (CP 15/2017- Programa de Bolsas de Produtividade em Pesquisa e Desenvolvimento Tecnológico) and to CNPq (process number 421541/2018-0, Chamada Universal MCTIC/CNPq n.° 28/2018).info:eu-repo/semantics/publishedVersio

RELATO DE CASO COM NOVO PROTOCOLO DE ATENDIMENTO PARA A CONFECÇÃO DE PRÓTESES TOTAIS NO CEO REGIONAL DE QUIXERAMOBIM

O edentulismo é um agravo de saúde que atinge mais de 3 milhões de idosos entre 65 e 74 anos no Brasil

A relação entre a experiência do paciente, o perfil sociodemográfico e a não adesão à hospitalização / The relationship between patient experience, sociodemographic profile, and nonadherence to hospitalization

As políticas e decisões em saúde devem ser pensadas para além da prática técnica de excelência, o que torna a experiência do paciente um importante indicador da qualidade da assistência em saúde. É necessário, a partir desta perspectiva, analisar os diversos fatores que influenciam a assistência em saúde e a decorrente experiência do paciente com esta assistência. Um destes fatores é a não adesão ao tratamento médico. Nesse sentido, uma questão que demanda investigação é quais são os fatores da experiência do paciente que se relacionam com a não adesão ao tratamento expressa através da saída do paciente do hospital com parecer médico desfavorável. Desta forma, o presente estudo buscou compreender a relação entre dados sociodemográficos, a experiência do paciente e a saída do hospital devido à não adesão à hospitalização em um hospital de urgência e emergência. A amostra foi composta por 254 prontuários de pacientes atendidos nesta instituição, sendo que 67 foram classificados como “risco de evasão''. A pesquisa possui caráter quantitativo, transversal e retrospectivo. Os dados foram analisados através da frequência absoluta e relativa para identificar as variáveis relacionadas ao risco de evasão. Os dados coletados demonstram a prevalência do risco de evasão entre homens jovens, casados/com união estável, autônomos e com baixo nível instrucional

Use of nanoencapsulated curcumin against vegetative cells and spores of Alicyclobacillus spp. in industrialized orange juice

Pathogenic and deteriorating bacteria are a great concern to food safety. In this sense, the present study evaluated the fight against microbial contamination through the use of nanoparticles containing curcumin, in addition to analyzing the physical properties of these nanoparticles. Efficient curcumin encapsulation was determined by Fourier transform infrared spectra evaluation and differential scanning calorimetry. Transmission electron microscopy images showed irregular shaped nanoparticles with broad size distribution (20–250 nm). The antibacterial activity was considered satisfactory, since curcumin in the form of nanoparticles demonstrated antimicrobial and antibacterial activity superior to curcumin in its free form, against both pathogenic bacteria, such as Staphylococcus aureus (MIC 125 μg/mL), and deteriorates, such as Alicyclobacillus acidoterrestris (MIC 62.5 μg/mL). Since curcumin nanoparticles may be consumed as a food additive, the bioactive properties of the nanoencapsulated curcumin were also evaluated in relation to antioxidant capacity (Thiobarbituric acid reactive substances (TBARS) and oxidative hemolysis inhibition assays) and cytotoxicity against four carcinoma cell lines, as well as two non-tumor cells. As a proof of concept, nanoparticles were incorporated in orange juice, with the juice maintaining satisfactory pH, °Brix, and color stability, during three days of storage (8 °C).This study was financed in part by the Coordenaç˜ao de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001. The authors thank the “Central Analítica Multiusu´ario da UTFPR Campo Mourão” (CAMulti-CM) for the analyses. Fernanda V. Leimann (process 039/2019) would like to thank Fundação Araucária (CP 15/2017- Programa de Bolsas de Produtividade em Pesquisa e Desenvolvimento Tecnológico) and CNPq (process number 421541/2018-0, Chamada Universal MCTIC/CNPq n◦ 28/2018). The authors are also grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES to CIMO (UIDB/00690/ 2020); L. Barros and C. Pereira also thank FCT, P.I., through the institutional scientific employment program-contract.info:eu-repo/semantics/publishedVersio

Analytical validation of an ultraviolet–visible procedure for determining vitamin D3 in vitamin D3-loaded microparticles and toxigenetic studies for incorporation into food

Vitamin D is a water-insoluble compound presented in two main forms (D2 and D3), susceptible to environmental conditions. Microencapsulation is an alternative to supplements and preserve vitamin D properties in foods. Entrapment efficiency (EE) is the main property to evaluate the encapsulation effectiveness and therefore it is of interest the study of analytical methods for the identification and quantification of this compound within the particle. This paper describes a low cost UV–Vis methodology validation to the identification and quantification of vitamin D3 in microparticles produced by hot homogenization. The method was validated following the International Conference on Harmonization (ICH) guidelines. To guarantee safe application in foodstuff, microparticles toxigenicity was evaluated with Allium cepa L. in vivo model, showing no cytotoxic nor genotoxic potential. High entrapment efficiency was obtained, the results also demonstrated that the concentration of vitamin D3 in microparticles can be safely accessed by the validated method.This study was financed in part by the Coordenaç˜ao de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES) – Finance Code 001. The authors thank the Multiuser Laboratory of Federal University of Technology – Paraná – Campus Londrina, and the “Central Analítica Multiusuário da UTFPR Campo Mourão” (CAMulti-CM) for the analyses. Also, to CNPq for the financial support (Project n◦ 420055/2018-5) and scholarship.info:eu-repo/semantics/publishedVersio

Soy protein isolate films incorporated with pinhão (Araucaria angustifolia (Bertol.) Kuntze) extract for potential use as edible oil active packaging

One of the traditional residues obtained from pinhão seeds (Araucaria angustifolia (Bertol.) Kuntze) consists of an aqueous extract produced from the cooking process, which presents a significant concentration of phenolic compounds with antioxidant properties. In this work, soy protein isolate (SPI) films with different concentrations of pinhão cooking water extract (EP, 0.5, 1, and 2% wt/wt) were produced and physical properties, microstructure, and antioxidant capacity were investigated. The films were applied as packaging (as sachet-type) for linseed oil, and the oil oxidative stability was evaluated during 10 days under accelerated storage condition (60 °C) by conventional procedures (peroxide index, specific extinction coefficient, and UV-Vis spectrophotometry) and by multivariate curve resolution with alternating least squares (MCR-ALS) chemometric method. The film with EP contributed to the oxidative stability of linseed oil being an interesting alternative of active biodegradable packaging for edible oils.The authors thank the CNPq for the financial support (project no. 420055/2018-5) and scholarship. This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001 (Masters Scholarships). The authors are also grateful to the Foundation for Science and Technology (FCT, Portugal) and FEDER under Programme PT2020 for financial support to CIMO (UID/AGR/00690/2019); national funding by FCT, P.I., through the institutional scientific employment program-contract for M.I. Dias and L. Barros contracts; and to FEDER-Interreg España-Portugal program for financial support through the project 0377_Iberphenol_6_E.info:eu-repo/semantics/publishedVersio

Araucaria angustifolia (Bertol.) Kuntze extract as a source of phenolic compounds in TPS/PBAT active films

There is growing interest in the development of biodegradable packaging materials containing natural antioxidant extracts. In this sense, the use of extracts obtained from agro-industrial byproducts has proved to be a sustainable alternative. In this study, Pinhão extract, a byproduct of Pinhão (Araucaria angustifolia (Bertol.) Kuntze) seed consumption, was characterized by HPLC-DAD-ESI/MSn, demonstrating the presence of eight phenolic compounds, (+)-catechin and (−)-epicatechin being the most abundant molecules. TPS/PBAT films containing Pinhão extract were produced by blown extrusion and their properties (tensile properties, thermal characteristics and microstructure) were evaluated in order to determine the effect of the presence of extracts. Results suggested that the interaction between the phenolic compounds of the extract and the polymeric matrix caused the reduction in the crystallinity degree, and an increase in the starch glass transition temperature. The presence of Araucaria angustifolia (Bertol.) Kuntze extract significantly (p < 0.05) affected the color and opacity of the film. Regarding water vapor permeation, no significant difference (p > 0.05) was detected. However, the water solubility and the contact angle with water (polar solvent) and diiodomethane (non-polar solvent) significantly changed due to the extract addition. Moreover, the Pinhão extract conferred significant antioxidant capacity to the TPS/PBAT films as determined by DPPH, suggesting that this material can be applied as an active packaging material.The authors thank CNPq (Chamada Universal – MCTI/CNPq no. 14/2014, Processo 447768/2014-0), CAPES (Master’s scholarship) and Fundação Araucária (Programa Universal/ Pesquisa Básica e Aplicada 24/2012, protocolo 7334133700514041013) for the financial support and they also thank Associate Laboratory LSRE-LCM – UID/EQU/50020/2019 – funded by national funds through FCT/MCTES (PIDDAC) and FEDER under Programme PT2020 for financial support to CIMO (UID/AGR/00690/2019) and to the national funding by FCT, P.I., through the institutional scientific employment program-contract for L. Barros contracts. The authors are also grateful to FEDER-Interreg España-Portugal programme for financial support through the project 0377_Iberphenol_6_E.info:eu-repo/semantics/publishedVersio