The structure of a purple acid phosphatase involved in plant growth and pathogen defence exhibits a novel immunoglobulin-like fold

Phosphatases function in the production, transport and recycling of inorganic phosphorus, which is crucial for cellular metabolism and bioenergetics, as well as in bacterial killing, since they are able to generate reactive oxygen species via Fenton chemistry. Diphosphonucleotide phosphatase/phosphodiesterase (PPD1), a glycoprotein plant purple acid phosphatase (PAP) from yellow lupin seeds, contains a bimetallic Fe–Mn catalytic site which is most active at acidic pH. Unlike other plant PAPs, PPD1 cleaves the pyrophosphate bond in diphosphonucleotides and the phosphodiester bond in various phosphodiesters. The homohexameric organization of PPD1, as revealed by a 1.65 Å resolution crystal structure and confirmed by solution X-ray scattering, is unique among plant PAPs, for which only homodimers have previously been reported. A phosphate anion is bound in a bidentate fashion at the active site, bridging the Fe and Mn atoms in a binding mode similar to that previously reported for sweet potato PAP, which suggests that common features occur in their catalytic mechanisms. The N-terminal domain of PPD1 has an unexpected and unique fibronectin type III-like fold that is absent in other plant PAPs. Here, the in vitro DNA-cleavage activity of PPD1 is demonstrated and it is proposed that the fibronectin III-like domain, which `overhangs' the active site, is involved in DNA selectivity, binding and activation. The degradation of DNA by PPD1 implies a role for PPD1 in plant growth and repair and in pathogen defence

Parameter Study of Star-Discs Encounters

Interactions between disc-surrounded stars might play a vital role in the formation of planetary systems. Here a first parameter study of the effects of encounters on low-mass discs is presented. The dependence of the mass and angular momentum transport on the periastron distance, the relative mass of the encountering stars and eccentricity of the encounter is investigated in detail. This is done for prograde and retrograde coplanar encounters as well as non-coplanar encounters. For distant coplanar encounters our simulation results agree with the analytical approximation of the angular momentum loss by Ostriker(1994). However, for close or high-mass encounters, significant differences to this approximation are found. This is especially so in the case of retrograde encounters, where the analytical result predict no angular momentum loss regardless of the periastron distance whereas the simulations find up to ~ 20% loss for close encounters. For the non-coplanar case a more complex dependency on the inclination between orbital path and disc plane is found than for distant encounters. For the coplanar prograde case new fitting formulae for the mass and angular momentum loss are obtained, which cover the whole range from grazing to distant encounters. In addition, the final disc size and the mass exchange between discs is examined, demonstrating that for equal mass stars in encounters as close as 1.5 the disc radius, the disc size only is reduced by approximately 10%.Comment: 11 pages, 9 figure

James Webb Space Telescope Primary Mirror Integration: Testing the Multiwavelength Interferometer on the Test Bed Telescope

The James Webb Space Telescope (JWST) integration includes a center of curvature test on its 18 primary mirror segment assemblies (PMSAs). This important test is the only ground test that will demonstrate the ability to align all 18 PMSAs. Using a multi-wavelength interferometer (MWIF) integrated to the test bed telescope (TBT), a one-sixth scale model of the JWST, we verify our ability to align and phase the 18 PMSAs. In this paper we will discuss data analysis and test results when using the MWIF to align the segments of the TBT in preparation for alignment of the JWST

Optical nulling apparatus and method for testing an optical surface

An optical nulling apparatus for testing an optical surface includes an aspheric mirror having a reflecting surface for imaging light near or onto the optical surface under test, where the aspheric mirror is configured to reduce spherical aberration of the optical surface under test. The apparatus includes a light source for emitting light toward the aspheric mirror, the light source longitudinally aligned with the aspheric mirror and the optical surface under test. The aspheric mirror is disposed between the light source and the optical surface under test, and the emitted light is reflected off the reflecting surface of the aspheric mirror and imaged near or onto the optical surface under test. An optical measuring device is disposed between the light source and the aspheric mirror, where light reflected from the optical surface under test enters the optical measuring device. An imaging mirror is disposed longitudinally between the light source and the aspheric mirror, and the imaging mirror is configured to again reflect light, which is first reflected from the reflecting surface of the aspheric mirror, onto the optical surface under test

Wavefront Calibration Testing of the James Webb Space Telescope Primary Mirror Center of Curvature Optical Assembly

The James Webb Space Telescope (JWST) Optical Telescope Element (OTE) consists of a 6.6 meter clear aperture, all-reflective, three-mirror anastigmat!. The 18-segment primary mirror (PM) presents unique and challenging assembly, integration, alignment and testing requirements. A full aperture center of curvature optical test is performed in cryogenic vacuum conditions at the integrated observatory level to verify PM performance requirements. Two wavefront calibration tests are utilized to verify the low and Mid/High spatial frequency performance ofthe test system. In this paper the methods and results of the wavefront calibration tests are presented

Measuring Segmented Primary Mirror WFE in the Presence of Vibration and Thermal Drift on the Light-Weighted JWST

The light-weighted design of the Optical Telescope Element (OTE) of the James Webb Telescope (JWST) leads to additional sensitivity to vibration from the ground - an important consideration to the measurement uncertainty of the wavefront error (WFE) in the primary mirror. Furthermore, segmentation of the primary mirror leads to rigid-body movements of segment areas in the WFE. The ground vibrations are minimized with modifications to the test facility, and by the architecture of the equipment supporting the load. Additional special test equipment (including strategically placed isolators, tunable mass dampers, and cryogenic magnetic dampers) mitigates the vibration and the response sensitivity before reaching the telescope. A multi-wavelength interferometer is designed and operated to accommodate the predicted residual vibration. Thermal drift also adds to the measurement variation. Test results of test equipment components, measurement theory, and finite element analysis combine to predict the test uncertainty in the future measurement of the primary mirror. The vibration input to the finite element model comes from accelerometer measurements of the facility with the environmental control pumps operating. One of the isolators have been built and tested to validate the dynamic performance. A preliminary model of the load support equipment and the OTE with the Integrated Science Instrument Module (ISIM) is complete. The performance of the add-on dampers have been established in previous applications. And operation of the multi-wavelength interferometer was demonstrated on a scaled hardware version of the JWST in an environment with vibration and thermal drift

Correlations between selected parameters of nasal cavity in neonates and young infants — computed tomography study

  Background: Correlations between selected metric parameters of the nasal cavity and nasopharynx in children without atresia may be useful for anticipating probable dimensions of this region in living subjects, in terms of changes with age. Materials and methods: One hundred and eighty children, age range 0–3 years, were divided into five age groups, and measurements of 18 distances between structures of nasal cavity and nosopharynx were performed on computed tomography scans. Correlation coefficients and relations between parameters have been determined. Results: Our study confirmed the existence of statistically significant correlations between linear dimensions within nasal cavity in children. The analysis demon­strated that for the values of following indexes: nasal septum length/piriform aperture width, and maximum length of the nasal septum/posterior nares width no statistically significant differences have been noted between age groups of children. All correlations have been positive. No statistically significant differences have been noted between the maximum width of the vomer and osseous parameters measured, both in the anterior and posterior part of the nasal cavity, and nasal septum length. Conclusions: The size of posterior nares changed with age in children by a constant value. So far, no such an analysis has been carried out assessing potential correlations between linear dimensions for the entire nasal cavity, nasopharynx, length of the nasal septum in children, as well as proportions of individual linear dimensions of the anatomical structures analysed, in various age groups

HmuY Haemophore and Gingipain Proteases Constitute a Unique Syntrophic System of Haem Acquisition by Porphyromonas gingivalis

Haem (iron protoporphyrin IX) is both an essential growth factor and virulence regulator for the periodontal pathogen Porphyromonas gingivalis, which acquires it mainly from haemoglobin via the sequential actions of the R- and K-specific gingipain proteases. The haem-binding lipoprotein haemophore HmuY and its cognate receptor HmuR of P. gingivalis, are responsible for capture and internalisation of haem. This study examined the role of the HmuY in acquisition of haem from haemoglobin and the cooperation between HmuY and gingipain proteases in this process. Using UV-visible spectroscopy and polyacrylamide gel electrophoresis, HmuY was demonstrated to wrest haem from immobilised methaemoglobin and deoxyhaemoglobin. Haem extraction from oxyhaemoglobin was facilitated after oxidation to methaemoglobin by pre-treatment with the P. gingivalis R-gingipain A (HRgpA). HmuY was also capable of scavenging haem from oxyhaemoglobin pre-treated with the K-gingipain (Kgp). This is the first demonstration of a haemophore working in conjunction with proteases to acquire haem from haemoglobin. In addition, HmuY was able to extract haem from methaemalbumin, and could bind haem, either free in solution or from methaemoglobin, even in the presence of serum albumin

Observational indicators of the transition from fully convective stars to stars with radiative cores

We present a discussion of the similarities and key differences between the transition onto (at the turn-on) and away from (at the turn-off) the main sequence, the latter termed the Hertzsprung gap. Using a set of model isochrones and adopting an initial mass function leads us to predict a dearth of G-type stars for any star forming region. This is caused by the (relatively) constant spectral type at which the transition from a fully convective star to a star with a radiative core begins. We also present analysis of the details of this transition in the ONC. In particular we show that a gap in the photometric and spectral type distributions is centred on, and a change in the fractional X-ray luminosity and rotation rate distribution occurs approximately at, the position of a peak in radiative core size as a function of mass. Whilst photometric signatures of this transition are lost at ages over ~20 Myrs, we show that changes in fractional X-ray luminosity and magnetic field configuration persist to older ages. Analysis of literature data show that the mass at which the change in fractional X-ray luminosity is observed decreases with age.Comment: 9 pages and 6 figures. Accepted for publication in MNRA

Characterization of the JWST Pathfinder Mirror Dynamics Using the Center of Curvature Optical Assembly (CoCOA)

The JWST (James Webb Space Telescope) Optical Telescope Element (OTE) consists of a 6.6 meter clear aperture, 18-segment primary mirror, all-reflective, three-mirror anastigmat operating at cryogenic temperatures. To verify performance of the primary mirror, a full aperture center of curvature optical null test is performed under cryogenic conditions in Chamber A at NASA Johnson Space Center using an instantaneous phase measuring interferometer. After phasing the mirrors during the JWST Pathfinder testing, the interferometer is utilized to characterize the mirror relative piston and tilt dynamics under different facility configurations. The correlation between the motions seen on detectors at the focal plane and the interferometer validates the use of the interferometer for dynamic investigations. The success of planned test hardware improvements will be characterized by the multi-wavelength interferometer (MWIF) at the Center of Curvature Optical Assembly (CoCOA)