Rast i senzorni pokazatelji afričkog soma (Clarias gariepinus) hranjenog subletalnom dozom ekstrakta lišća nima (azadirahte) i njegovi antibakterijski učinci

The antibacterial effects of Neem leaf, the growth performance and sensory qualities of African catfish fed with Neem leaf inclusion were investigated in this study. Ninety (90) post-juvenile Clarias gariepinus (42.40 ± 2.50 g) were used. Experimental groups in triplicate were exposed bi-weekly to 3.5% and 7% LC50 of Neem leaves aqueous extract infused in commercial floating feed for four weeks, while the control group was exposed to untreated feed. The whole body length and weight of randomly sampled fish were taken for organo-somatic indices. Gills and skin samples were collected for bacteriological screening, while fish muscles were prepared for sensory acceptability tests. Mean feed intake was significantly (P<0.05) influenced by Neem leaf in the three treatment concentrations with an insignificant difference between the treated groups, but it was higher than in the control group. Mean weight gain was significantly higher (P<0.05) in the Neem fed fish than the control but insignificant the differences between treatment doses were insignificant, while the feed conversion ratio was significantly lower (P<0.05) in the Neem fed groups compared to the control, but the difference was insignificant between the treatment doses. All groups were apparently healthy looking with 100% survival. There was a considerable reduction in total bacterial count in both the gills and skin with the increase in Neem concentration. Micrococci spp. and Bacillus subtilis were not isolated in the gills but were present in the skin, while E. coli and Pseudomonas fluorescens were not suppressed in the skin. There was no significant difference (P<0.05) in all the sensory parameters across all groups. The inclusion of Neem leaf extract was shown to have a high antibacterial effect coupled with excellent growth performance and general acceptability of fish tissue.U ovom su radu istraženi antibakterijski učinci lišća nima, kao i rast i senzorni pokazatelji afričkog soma (Clarias gariepinus) hranjenog ovim lišćem. Upotrijebljeno je 90 odraslih afričkih somova (42,40 ± 2,50 g). Pokusna je skupina izložena tijekom dva tjedna 3,5 % i 7 % LC50 vodenom ekstraktu lišća nima dodanog u komercijalnu hranu tijekom četiri tjedna, dok je kontrolna skupina hranjena netretiranom hranom. Određena je tjelesna dužina i masa nasumično odabranih riba za provjeru organoleptičkih pokazatelja. Škrge i uzorci kože prikupljeni su za bakteriološku analizu, dok su uzorci mišića pripremljeni za senzornu analizu. Prosječna konzumacija hrane bila je pod znakovitim utjecajem (P < 0,05) lišća nima, neovisno o koncentraciji, u odnosu na kontrolnu skupinu. Prosječan prirast bio je znakovito viši (P < 0,05) u riba hranjenih lišćem nima u odnosu na kontrolnu skupinu, dok je konverzija hrane bila znakovito niža (P < 0,05) u skupini hranjenoj lišćem nima, neovisno o dozi. Ribe u svim skupinama bile su naizgled zdrave uz 100%-tno preživljenje. Utvrđen je zamjetan pad brojnosti ukupnih bakterija u škrgama i na koži s porastom koncentracije lišća nima. Bakterije Micrococci spp. i Bacillus subtilis nisu izolirane iz škrga, ali su utvrđene u koži, kao i E. coli i Pseudomonas fluorescens. Nisu utvrđene znakovite razlike senzornih pokazatelja. Dodatak lišća nima ima visok antibakterijski učinak praćen odličnim pokazateljima rasta te općom prihvatljivošću tkiva ribe za konzumaciju

Efficacy of intervention strategies for bioremediation of crude oil in polluted soil microcosm

Crude oil, though not manmade but largely manipulated by man to provide different oil-based products has become a major source of environmental pollution. This menace on land do contribute to the retardation of vegetation growth and human health hazards, while in water it may be toxic to aquatic animals. The search for the solution to ameliorate the seemingly unending pollution and its side effects necessitated the evaluation on the effect of bioaugmentation, biostimulation and natural attenuation of crude oil pollution in soil microcosms. The bacterial species selected for this study (Bacillus thuringensis strain LG32 and Burkolderia pseudomallei strain A81) were preliminarily identified using the conventional biochemical tests and further identification was carried out using the API kit. The results of the study carried out over a period of five weeks indicated that there was a marked reduction in the available phosphorous and potassium in the bioaugmented and biostimulated soils compared with that of the control. The mean values for total viable counts (TVC) of population of hydrocarbon utilizing bacteria (HUB) was higher in the bioaugmented soil ranged (LG32=6.0-7.5log10cfu g-1 ; A81=5.5- 7.5log10cfug-1 ; LG32+A81=6.0-7.5log10cfug-1 ) compared with that of the control (6.0-6.2log10cfug-1 ). When bioaugmentation was combined with biostimulation, the soil had higher counts of HUB (6.0-9.0log10cfug-1 ) and HUF (3.5-6.5log10cfug-1) compared to bioaugmentation without stimulation (HUB: 6.0-7.5; HUF: 3.5-5.5). The GC result indicated that by day 35, 96.92% of the aliphatic and aromatic components have been degraded in the augmented soil, higher than the natural attenuation control

Potentials of microorganisms associated with plantain peels in the Lagos metropolis for biodegradation and bioconversion.

The role of microbes in the degradation of plantain derived-wastes and their potential to produce cellulolytic enzymes was assessed. Soil samples of decomposing waste piles were collected from two major plantain markets in the Lagos metropolis and analyzed for physicochemical properties, toxic heavy metal content and microbial populations. Findings revealed that the values of moisture content of the two soils varied between 7.27±0.04 and 8.06±0.19 %. M-12 site had the highest organic matter content of 6.89±0.14 %. A similar pattern was observed for nitrate, phosphate and chloride levels while some heavy metals were also detected in varying and high amounts. The highest viable bacterial counts was 58.0±2.9 x 104 cfu/g at MU and there were no fungi at the site whereas M-12 had a fungal count of 40.0±3.3 x 103 cfu/g. Out of the total of 34 isolates encountered, 8 isolates having maximum cellulase activities were selected for further studies by the primary screening technique. These test organisms were then evaluated by secondary screening for enzyme production. The test organisms were phenotypically and biochemically characterized and identified as Klebsiella pneumoniae spp pneumoniae (2 strains), Klebsiella pneumoniae spp ozaenae, Enterobacter aerogenes, Providencia alcalifaciens, Aspergillus flavus, Aspergillus fumigatus and Aspergillus niger respectively. Both the bacteria and moulds were found to be capable of utilizing lignin and cellulosic substrates for growth and for production of cellulolytic enzymes. It is suggested that such microorganisms could be useful in bioconversion of cellulosic substrates like plantain-derived wastes for biotechnological application

Community engagement and compliance monitoring of COVID-19 safety protocols: innovative approach combining indigenous practice and GIS technology in Oyo State, Nigeria

Background: One of the major challenges that has driven the spread of Coronavirus Disease 2019 (COVID-19) worldwide is the burden of enforcing the preventive measures required to contain the pandemic. Enforcement of COVID-19 precautionary behaviour should not be homogenous; every country needs to be creative to ensure that humane considerations guide all decisions during the extraordinary experience that COVID-19 pandemic portends. The model of self-policing is acceptable and maintained principally because the citizens of any communities operate, recognize, and accept them as preferred alternatives to the official models of policing for enforcement. Hence the approach presented in this paper, which deployed existing indigenous alternative systems in ensuring compliance with COVID-19 precautionary behaviour. This article therefore documents the unique approach deployed for the containment of COVID-19 in Oyo State, Nigeria. Objective: This intervention was designed to explore established indigenous alternative systems and models of control, justice, law, security, and enforcement in Nigeria. Additionally, geographic information system (GIS) technology and investigative journalism was used to monitor and evaluate the effectiveness of the intervention. Method: The method employed was community conversation; a method of increasing inclusive, community-based engagement harnessing the expertise and motivation of key stakeholders. The community conversations were convened after the pattern of a traditional Town-hall meeting. Community conversations were organized as a qualitative framework focusing on deploying the indigenous practice of self-policing associated with Nigerias trade unions and aims to inform COVID-19 preventive behaviour at the community level. Geographical information system technology was used to develop COVID-19 Containment Compliance Citizens Reporter App. The App was developed using ESRI ArcGIS online platform to crowd source public feedback on compliance or contravention of COVID-19 protocols. Social media platforms were also deployed for monitoring and evaluation of the intervention post townhall meeting. Results: The establishment of a State-wide Containment response network provided the required inroad for advocacy and deployment of state-wide community conversation framework in the different communities comprising diverse ethnic groups, religious leaders, market leaders, National Union of Road Transport Workers (NURTW), and so on. Testimonials from the various communities showed that the people have embraced the self-policing strategy and the network system was effective with good outcomes in terms of response to decontamination, containment, and advocacy. The COVID-19 Containment Compliance Citizens Reporter App, investigative reporting by mass media were highly effective tools for monitoring and evaluation of the outcome of the intervention as well as possible evidence for melting out incentive and disincentive measures as necessary. This approach is a template, which could be adapted and replicated in other parts of Nigeria and other African societies with similar structures, demographics, and indigenous practices

Burnout among surgeons before and during the SARS-CoV-2 pandemic: an international survey

Background: SARS-CoV-2 pandemic has had many significant impacts within the surgical realm, and surgeons have been obligated to reconsider almost every aspect of daily clinical practice. Methods: This is a cross-sectional study reported in compliance with the CHERRIES guidelines and conducted through an online platform from June 14th to July 15th, 2020. The primary outcome was the burden of burnout during the pandemic indicated by the validated Shirom-Melamed Burnout Measure. Results: Nine hundred fifty-four surgeons completed the survey. The median length of practice was 10&nbsp;years; 78.2% included were male with a median age of 37&nbsp;years old, 39.5% were consultants, 68.9% were general surgeons, and 55.7% were affiliated with an academic institution. Overall, there was a significant increase in the mean burnout score during the pandemic; longer years of practice and older age were significantly associated with less burnout. There were significant reductions in the median number of outpatient visits, operated cases, on-call hours, emergency visits, and research work, so, 48.2% of respondents felt that the training resources were insufficient. The majority (81.3%) of respondents reported that their hospitals were included in the management of COVID-19, 66.5% felt their roles had been minimized; 41% were asked to assist in non-surgical medical practices, and 37.6% of respondents were included in COVID-19 management. Conclusions: There was a significant burnout among trainees. Almost all aspects of clinical and research activities were affected with a significant reduction in the volume of research, outpatient clinic visits, surgical procedures, on-call hours, and emergency cases hindering the training. Trial registration: The study was registered on clicaltrials.gov "NCT04433286" on 16/06/2020

Changes in preterm birth and stillbirth during COVID-19 lockdowns in 26 countries.

Preterm birth (PTB) is the leading cause of infant mortality worldwide. Changes in PTB rates, ranging from -90% to +30%, were reported in many countries following early COVID-19 pandemic response measures ('lockdowns'). It is unclear whether this variation reflects real differences in lockdown impacts, or perhaps differences in stillbirth rates and/or study designs. Here we present interrupted time series and meta-analyses using harmonized data from 52 million births in 26 countries, 18 of which had representative population-based data, with overall PTB rates ranging from 6% to 12% and stillbirth ranging from 2.5 to 10.5 per 1,000 births. We show small reductions in PTB in the first (odds ratio 0.96, 95% confidence interval 0.95-0.98, P value <0.0001), second (0.96, 0.92-0.99, 0.03) and third (0.97, 0.94-1.00, 0.09) months of lockdown, but not in the fourth month of lockdown (0.99, 0.96-1.01, 0.34), although there were some between-country differences after the first month. For high-income countries in this study, we did not observe an association between lockdown and stillbirths in the second (1.00, 0.88-1.14, 0.98), third (0.99, 0.88-1.12, 0.89) and fourth (1.01, 0.87-1.18, 0.86) months of lockdown, although we have imprecise estimates due to stillbirths being a relatively rare event. We did, however, find evidence of increased risk of stillbirth in the first month of lockdown in high-income countries (1.14, 1.02-1.29, 0.02) and, in Brazil, we found evidence for an association between lockdown and stillbirth in the second (1.09, 1.03-1.15, 0.002), third (1.10, 1.03-1.17, 0.003) and fourth (1.12, 1.05-1.19, <0.001) months of lockdown. With an estimated 14.8 million PTB annually worldwide, the modest reductions observed during early pandemic lockdowns translate into large numbers of PTB averted globally and warrant further research into causal pathways

Changes in preterm birth and stillbirth during COVID-19 lockdowns in 26 countries.

Preterm birth (PTB) is the leading cause of infant mortality worldwide. Changes in PTB rates, ranging from -90% to +30%, were reported in many countries following early COVID-19 pandemic response measures ('lockdowns'). It is unclear whether this variation reflects real differences in lockdown impacts, or perhaps differences in stillbirth rates and/or study designs. Here we present interrupted time series and meta-analyses using harmonized data from 52 million births in 26 countries, 18 of which had representative population-based data, with overall PTB rates ranging from 6% to 12% and stillbirth ranging from 2.5 to 10.5 per 1,000 births. We show small reductions in PTB in the first (odds ratio 0.96, 95% confidence interval 0.95-0.98, P value <0.0001), second (0.96, 0.92-0.99, 0.03) and third (0.97, 0.94-1.00, 0.09) months of lockdown, but not in the fourth month of lockdown (0.99, 0.96-1.01, 0.34), although there were some between-country differences after the first month. For high-income countries in this study, we did not observe an association between lockdown and stillbirths in the second (1.00, 0.88-1.14, 0.98), third (0.99, 0.88-1.12, 0.89) and fourth (1.01, 0.87-1.18, 0.86) months of lockdown, although we have imprecise estimates due to stillbirths being a relatively rare event. We did, however, find evidence of increased risk of stillbirth in the first month of lockdown in high-income countries (1.14, 1.02-1.29, 0.02) and, in Brazil, we found evidence for an association between lockdown and stillbirth in the second (1.09, 1.03-1.15, 0.002), third (1.10, 1.03-1.17, 0.003) and fourth (1.12, 1.05-1.19, <0.001) months of lockdown. With an estimated 14.8 million PTB annually worldwide, the modest reductions observed during early pandemic lockdowns translate into large numbers of PTB averted globally and warrant further research into causal pathways

Antibiotic Sensitivity Pattern of Pathogenic Bacterial Isolates From Diseased Clarias gariepinus From Selected Ibadan And Ikorodu Farms

This study was carried out to isolate, characterise and identify bacteria from diseased Clarias gariepinusand also assess the occurrence of resistance to antimicrobial in isolated bacteria. Samples of diseased African Catfish were collected for a period of six weeks from consenting farms in Ibadan and Ikorodu in Nigeria and examined for clinical signs of disease. Pond water samples along with organs such as gills, skin, intestine, kidney and lungs from these fish were analyzed microbiologically using differential and selective media. Bacteria enumeration, identification and biochemical characterization were carried out and the physiochemical parameters of the water samples determined. All isolates were subjected to antibiotic sensitivity test using the standard Kirby-Bauer disc diffusion method. The total bacterial load for the organs ranged between 3.0 x 104 (lungs sample) and 6.0 x 107cfu/g (gill sample). The gills had the highest average total bacterial count, while lungs had the least. Morphologically unique bacterial isolates obtained included Salmonella (14 isolates), Pseudomonas (4 isolates), Aeromonas (2 isolates), Edwardsiella (3 isolates) and Shigella (3 isolates). These isolates displayed antibiotic resistance profile to the following: Ceftazidime (38%), Cefuroxime (77%), Gentamicin (37%), Cefixime (73%), Ofloxacin (23%), Augmentin (66%), Nitrofurantoin (58%) and Ciprofloxacin (15%). Two Salmonella isolates had multi-drug resistance pattern. This study showed that indiscriminate use of unlicensed or unapproved antibiotics for aquaculture portends significant hazards to public health therefore disease prevention is preferable through good culture and health management to ensure optimum yields and wholesome products

Different organic manure sources and NPK fertilizer on soil chemical 3 properties, growth, yield and quality of okra

Use of organic manures to meet the nutrient requirement of crop would be an inevitable practice 29 in the years to come for sustainable agriculture since organic manures generally improve the soil 30 physical, chemical and biological properties. Hence, field experiments were carried out in 2017 31 and 2018 to compare the impact of different organic manures and NPK fertilizer on soil properties, 32 growth, yield, proximate and mineral contents of okra (Abelmoschus esculentus L.). The 33 treatments each year 2017 and 2018 consisted of: rabbit manure, cow dung, poultry manure, green 34 manure [Mexican sunflower (Tithonia diversifolia Asteraceae)], pig manure, NPK 15- 15-15 35 fertilizer applied at 120 kg N ha-1 and a control (no manure/ inorganic fertilizer). The seven 36 treatments were laid out in a randomized complete block design with three replication. Organic 37 manures and NPK fertilizer increased the soil organic matter (OM), N, P, K, Ca and Mg (NPK 38 fertilizer did not increase OM, Ca and Mg significantly), growth, yield, minerals, protein, ash, 39 carbohydrate and mucilage contents of okra fruit as compared with control. Organic manures 40 improved okra yield compared with NPK fertilizer. Okra growth and yield parameters were 41 significantly higher in 2018 compared with 2017. Control, rabbit manure, cow dung, poultry 42 manure, green manure, pig manure and NPK fertilizer in 2018 increased the pod yield of okra by 43 9.7%, 35.3%, 57.9%, 36.2%, 39.2%, 45.5% and 3.2%, respectively compare with the same 44 treatment in 2017. Amongst various organic manures, poultry manure produced significantly 45 higher plant growth, yield, mineral and proximate composition of okra because of its high soil 46 chemical properties which could be related to its lowest C: N ratio, lignin and lignin: N ratio. 47 Results also showed that okra grown during high intensity rainfall has higher yield but with 48 reduced quality except its mucilage content. Therefore, planting of okra with poultry manure under 49 moderate rainfall will enhance the health benefit from the fruit, however, those that desire its 50 mucilage content planting during high rainfall is recommended

A multi-state survey of farm-level preparedness towards African swine fever outbreak in Nigeria

Outbreaks of African Swine Fever (ASF) have severe economic implications for Nigeria and result in significant loss of livelihoods. The non-availability of vaccines makes biosecurity the reliable key to reducing ASF outbreaks. This study evaluated preparedness for ASF outbreaks at the farm level among 247 pig farmers randomly selected from Abia, Akwa-Ibom, Edo, Kwara, and Oyo states. We categorized each pig farmer's ASF preparedness rating (ASF - PR) as "poor", "moderate", and "satisfactory" based on their score on an 11-item scale. Finally, a multivariable logistic regression analysis was conducted to assess the association between the socio-demographic variables and farm-level ASF preparedness. The awareness of ASF among pig farmers was very high (87.9%, n = 217). Most farmers knew the clinical signs of the disease, the modes of transmission of ASF, and correctly identified the risk factors. They also considered the need for thorough cleaning and disinfection of piggeries (87.1%, n = 189), tightened biosecurity (85.7%, n = 186), culling all ASF-affected pigs (77.9%, n = 169) as well as the ban on the transport of pigs and their products (49.8%, n = 108) as very important in ASF control. Conversely, 27.6%, (n = 60) of the farmers thought ASF could affect humans, 12% (n = 27) of them openly discarded the carcasses of dead pigs, and there was a high antibiotic usage. Most of the pig farmers used antibiotics as prophylaxis (63.6%, n = 157), chemotherapeutics (66.4%, n = 164), growth promoters (15.4%, n = 38), and wrongly so, 13% (n = 32) of them thought that antibiotics could be used to prevent and treat ASF. At the farm level, two-thirds (68.8%, n = 170) of the farmers had strict movement restrictions, and 48.6% (n = 120) routinely quarantine new pigs before introduction into their herd. Across the five states, 36% (n = 89) of the farmers had witnessed sudden death with signs consistent with ASF amongst their pigs and only 10.1% (n = 27) had confirmatory ASF diagnosis. The mean score for the farm-level ASF-PR was 6.95 & PLUSMN; 2.7. Approximately onequarter of the 247 pig farmers had satisfactory ASF - PR that might help to prevent the incursion of ASF into their farms. Most farmers had moderate ASF - PR (59.5%, n = 147) whereas 17% (n = 42) had very poor ASF-PR and were most prone to an ASF outbreak. Of the sociodemographic variables, only age was significantly associated with farm-level ASF preparedness as older pig farmers especially those aged between 50 and 59 years (OR: 4.83; 95% CI: 1.10, 21.22; p = 0.037) were more likely to have satisfactory ASF - PR than the others. Our findings showed pig farmers were not adequately prepared and the next ASF outbreak could pose more significant threat to pig populations across Nigeria. Government should urgently establish minimum biosecurity measures and improve its ASF surveillance mechanisms for commercial and backyard pig production.Peer reviewe