Effect of ultrasound on herpes simplex virus infection in cell culture

<p>Abstract</p> <p>Background</p> <p>Ultrasound has been shown to increase the efficiency of gene expression from retroviruses, adenoviruses and adeno-associated viruses. The effect of ultrasound to stimulate cell membrane permeabilization on infection with an oncolytic herpes simplex virus type 1 (HSV-1) was examined.</p> <p>Results</p> <p>Vero monkey kidney cells were infected with HSV-1 and exposed to 1 MHz ultrasound after an adsorption period. The number of plaques was significantly greater than that of the untreated control. A combination of ultrasound and microbubbles further increased the plaque number. Similar results were obtained using a different type of HSV-1 and oral squamous cell carcinoma (SCC) cells. The appropriate intensity, duty cycle and time of ultrasound to increase the plaque number were 0.5 W/cm², 20% duty cycle and 10 sec, respectively. Ultrasound with microbubbles at an intensity of 2.0 W/cm², at 50% duty cycle, or for 40 sec reduced cell viability.</p> <p>Conclusion</p> <p>These results indicate that ultrasound promotes the entry of oncolytic HSV-1 into cells. It may be useful to enhance the efficiency of HSV-1 infection in oncolytic virotherapy.</p

Generation of high-power arbitrary-wave-form modulated inductively coupled plasmas for materials processing

金沢大学大学院自然科学研究科電子科学金沢大学工学部An arbitrary-wave-form modulated induction thermal plasma (AMITP) system was developed using a high-power semiconductor high-frequency power supply. The modulated high-power plasma is a breakthrough technique for controlling the temperature and the radical density in high-density plasmas. The arbitrary-wave-form modulation of the coil current enables more detailed control of the temperature of the high-density plasmas than the pulse-amplitude modulation that has already been developed. The Ar AMITP with intentionally modulated coil current could be generated at a power of 10-15 kW. Results showed that the Ar excitation temperature between the specified excitation levels was changed intentionally according to the modulation control signal. © 2007 American Institute of Physics

The 150th anniversary of Nagasaki University School of Medicine: recovery from the atomic disaster and evolution of the department of neurosurgery.

NAGASAKI IS LOCATED on the western edge of Japan, closer to the Asian continent. Because of this geographical proximity, Nagasaki became a gateway for the introduction of continental culture and civilization to Japan. After the port of Nagasaki was opened for trade with the Portuguese in 1571, Nagasaki had a central role in cultural exchange with the West and China until the latter half of the 19th century. As a result of the political situation, students came to Nagasaki from all over Japan to obtain information on Western science, especially in medicine, turning Nagasaki into a hub for modern academic studies. The first medical facility in Japan educating doctors in the Western style was founded in Nagasaki in 1857. Despite the tragedy of World War II, the medical school arose again. More than 10 000 physicians have completed their studies at the medical school since its founding. The Department of Neurosurgery at Nagasaki University had its origins within the Second Department of Surgery and became an independent department in 1973. The post of professor was assumed by Kazuo Mori and succeeded in 1991 by Shobu Shibata and in 2003 by Izumi Nagata, who holds the post at the time of this writing. Neurosurgery is dynamic and constantly changing at Nagasaki University with work in progress on technological, diagnostic, and surgical innovations that permit the treatment of highly complex cases. In 2007, the 150th anniversary of the founding of Nagasaki University School of Medicine was celebrated with a number of commemorative events

Laboratory study of concrete produced with admixtures intended to inhibit corrosion

Thesis (M.S.)--University of Hawaii at Manoa, 2005.Includes bibliographical references (leaves 120-121).xii, 282 leaves, bound ill. (some col.) 29 c