Volume limited dependent Galactic model parameters

We estimated 34 sets of Galactic model parameters for three intermediate latitude fields with Galactic longitudes l=60, l=90, and l=180, and we discussed their dependence on the volume. Also, we confirmed the variation of these parameters with absolute magnitude and Galactic longitude. The star samples in two fields are restricted with bright and unit absolute magnitude intervals, (4,5], and (5,6], whereas for the third field a larger absolute magnitude interval is adopted, (4,10]. The limiting apparent magnitudes of star samples are g=15 and g=22.5 mag which provide space densities within distances in the line of sight 0.9 and 25 kpc. The Galactic model parameters for the thin disc are not volume dependent. However, the ones for thick disc and halo do show spectacular trends in their variations with volume, except for the scalelength of the thick disc. The local space density of the thick disc increases, whereas the scaleheight of the same Galactic component decreases monotonically. However, both model parameters approach asymptotic values at large distances. The axial ratio of the halo increases abruptly for the volumes where thick disc is dominant, whereas it approaches an asymptotic value gradually for larger volumes, indicating a continuous transition from disclike structure to a spherical one at the outermost region of the Galaxy. The variation of the Galactic model parameters with absolute magnitude can be explained by their dependence on the stellar luminosity, whereas the variation with volume and Galactic longitude at short distances is a bias in analysis.Comment: 12 pages, including 8 figures and 5 tables, accepted for publication in PAS

The stellar metallicity distribution in intermediate latitude fields with BATC and SDSS data

Based on the Beijing-Arizona-Taiwan-Connecticut (BATC) and Sloan Digital Sky Survey (SDSS) photometric data, we adopt SEDs fitting Method to evaluate the metallicity distribution for \sim40,000 main-sequence stars in the Galaxy. According to the derived photometric metallicities of these sample stars, we find that the metallicity distribution shift from metal-rich to metal-poor with the increase of distance from the Galactic center. The mean metallicity is about of 1.5 \pm 0.2dex in the outer halo and 1.3 \pm 0.1 dex in the inner halo. The mean metallicity smoothly decreases from -0.4 to -0.8 in interval 0 < r \leq 5 kpc. The fluctuation in the mean metallicity with Galactic longitude can be found in interval 4 < r \leq 8 kpc. There is a vertical abundance gradients d[Fe/H]/dz\sim -0.21 \pm 0.05 dex kpc-1 for the thin disk (z \leq 2 kpc). At distance 2 < z \leq 5 kpc, where the thick disk stars are dominated, the gradients are about of -0.16 \pm0.06 dex kpc-1, it can be interpreted as a mixture of stellar population with different mean metallicities at all z levels. The vertical metallicity gradient is - 0.05 \pm0.04 dex kpc-1 for the halo (z > 5 kpc). So there is little or no metallicity gradient in the halo

The Information Of The Milky Way From 2MASS Whole Sky Star Count: The Structure Parameters

The Ks band differential star count of the Two Micron All Sky Survey (2MASS) is used to derive the global structure parameters of the smooth components of the Milky Way. To avoid complication introduced by other fine structures and significant extinction near and at the Galactic plane, we only consider Galactic latitude |b| > 30 degree data. The star count data is fitted with a threecomponent model: double exponential thin disk and thick disk, and a power law decay oblate halo. Using maximum likelihood the best-fit local density of thin disk is n0 = 0.030 +- 0.002 stars/pc^3. The best-fit scale-height and length of the thin disk are Hz1 = 360+-10 pc and Hr1 = 3.7+-1.0 kpc, and those of the thick disk are and Hz2 = 1020+-30 pc and Hr2 = 5.0+-1.0 kpc, the local thick-to-thin disk density ratio is f2 = 7+-1%. The best-fit axis ratio, power law index and local density ratio of the oblate halo are kappa = 0.55+-0.15, p = 2.6+-0.6 and fh = 0.20+-0:10%, respectively. Moreover, we find some degeneracy among the key parameters (e.g. n0,Hz1, f2 and Hz2). Any pair of these parameters are anticorrelated to each other. The 2MASS data can be well-fitted by several possible combinations of parameters. This is probably the reason that there is a wide range of values for the structure parameters in literature similar to this study. Since only medium and high Galactic latitude data are analyzed, the fitting is very insensitive to the scale-lengths of the disks.Comment: 25 pages, 4 figures, accepted by ApJ on July 15 201

Fluoromycobacteriophages for rapid, specific, and sensitive antibiotic susceptibility testing of Mycobacterium tuberculosis

Rapid antibiotic susceptibility testing of Mycobacterium tuberculosis is of paramount importance as multiple- and extensively- drug resistant strains of M. tuberculosis emerge and spread. We describe here a virus-based assay in which fluoromycobacteriophages are used to deliver a GFP or ZsYellow fluorescent marker gene to M. tuberculosis, which can then be monitored by fluorescent detection approaches including fluorescent microscopy and flow cytometry. Pre-clinical evaluations show that addition of either Rifampicin or Streptomycin at the time of phage addition obliterates fluorescence in susceptible cells but not in isogenic resistant bacteria enabling drug sensitivity determination in less than 24 hours. Detection requires no substrate addition, fewer than 100 cells can be identified, and resistant bacteria can be detected within mixed populations. Fluorescence withstands fixation by paraformaldehyde providing enhanced biosafety for testing MDR-TB and XDR-TB infections. © 2009 Piuri et al

Participatory learning and action cycles with women s groups to prevent neonatal death in low-resource settings: A multi-country comparison of cost-effectiveness and affordability.

WHO recommends participatory learning and action cycles with women's groups as a cost-effective strategy to reduce neonatal deaths. Coverage is a determinant of intervention effectiveness, but little is known about why cost-effectiveness estimates vary significantly. This article reanalyses primary cost data from six trials in India, Nepal, Bangladesh and Malawi to describe resource use, explore reasons for differences in costs and cost-effectiveness ratios, and model the cost of scale-up. Primary cost data were collated, and costing methods harmonized. Effectiveness was extracted from a meta-analysis and converted to neonatal life-years saved. Cost-effectiveness ratios were calculated from the provider perspective compared with current practice. Associations between unit costs and cost-effectiveness ratios with coverage, scale and intensity were explored. Scale-up costs and outcomes were modelled using local unit costs and the meta-analysis effect estimate for neonatal mortality. Results were expressed in 2016 international dollars. The average cost was $203 (range:$61-$537) per live birth. Start-up costs were large, and spending on staff was the main cost component. The cost per neonatal life-year saved ranged from$135 to $1627. The intervention was highly cost-effective when using income-based thresholds. Variation in cost-effectiveness across trials was strongly correlated with costs. Removing discounting of costs and life-years substantially reduced all cost-effectiveness ratios. The cost of rolling out the intervention to rural populations ranges from 1.2% to 6.3% of government health expenditure in the four countries. Our analyses demonstrate the challenges faced by economic evaluations of community-based interventions evaluated using a cluster randomized controlled trial design. Our results confirm that women's groups are a cost-effective and potentially affordable strategy for improving birth outcomes among rural populations

Synthesis, antitubercular activity and mechanism of resistance of highly effective thiacetazone analogues

Defining the pharmacological target(s) of currently used drugs and developing new analogues with greater potency are both important aspects of the search for agents that are effective against drug-sensitive and drug-resistant Mycobacterium tuberculosis. Thiacetazone (TAC) is an anti-tubercular drug that was formerly used in conjunction with isoniazid, but removed from the antitubercular chemotherapeutic arsenal due to toxic side effects. However, several recent studies have linked the mechanisms of action of TAC to mycolic acid metabolism and TAC-derived analogues have shown increased potency against M. tuberculosis. To obtain new insights into the molecular mechanisms of TAC resistance, we isolated and analyzed 10 mutants of M. tuberculosis that were highly resistant to TAC. One strain was found to be mutated in the methyltransferase MmaA4 at Gly101, consistent with its lack of oxygenated mycolic acids. All remaining strains harbored missense mutations in either HadA (at Cys61) or HadC (at Val85, Lys157 or Thr123), which are components of the bhydroxyacyl-ACP dehydratase complex that participates in the mycolic acid elongation step. Separately, a library of 31 new TAC analogues was synthesized and evaluated against M. tuberculosis. Two of these compounds, 15 and 16, exhibited minimal inhibitory concentrations 10-fold lower than the parental molecule, and inhibited mycolic acid biosynthesis in a dose-dependent manner. Moreover, overexpression of HadAB HadBC or HadABC in M. tuberculosis led to high level resistance to these compounds, demonstrating that their mode of action is similar to that of TAC. In summary, this study uncovered new mutations associated with TAC resistance and also demonstrated that simple structural optimization of the TAC scaffold was possible and may lead to a new generation of TAC-derived drug candidates for the potential treatment of tuberculosis as mycolic acid inhibitors

Protocol for the cost-consequence and equity impact analyses of a cluster randomised controlled trial comparing three variants of a nutrition-sensitive agricultural extension intervention to improve maternal and child dietary diversity and nutritional status in rural Odisha, India (UPAVAN trial)

BACKGROUND: Undernutrition causes around 3.1 million child deaths annually, around 45% of all child deaths. India has one of the highest proportions of maternal and child undernutrition globally. To accelerate reductions in undernutrition, nutrition-specific interventions need to be coupled with nutrition-sensitive programmes that tackle the underlying causes of undernutrition. This paper describes the planned economic evaluation of the UPAVAN trial, a four-arm, cluster randomised controlled trial that tests the nutritional and agricultural impacts of an innovative agriculture extension platform of women's groups viewing videos on nutrition-sensitive agriculture practices, coupled with a nutrition-specific behaviour-change intervention of videos on nutrition, and a participatory learning and action approach. METHODS: The economic evaluation of the UPAVAN interventions will be conducted from a societal perspective, taking into account all costs incurred by the implementing agency (programme costs), community and health care providers, and participants and their households, and all measurable outcomes associated with the interventions. All direct and indirect costs, including time costs and donated goods, will be estimated. The economic evaluation will take the form of a cost-consequence analysis, comparing incremental costs and incremental changes in the outcomes of the interventions, compared with the status quo. Robustness of the results will be assessed through a series of sensitivity analyses. In addition, an analysis of the equity impact of the interventions will be conducted. DISCUSSION: Evidence on the cost and cost-effectiveness of nutrition-sensitive agriculture interventions is scarce. This limits understanding of the costs of rolling out or scaling up programs. The findings of this economic evaluation will provide useful information for different multisectoral stakeholders involved in the planning and implementation of nutrition-sensitive agriculture programmes. TRIAL REGISTRATION: ISRCTN65922679 . Registered on 21 December 2016

Production of phi mesons at mid-rapidity in sqrt(s_NN) = 200 GeV Au+Au collisions at RHIC

We present the first results of meson production in the K^+K^- decay channel from Au+Au collisions at sqrt(s_NN) = 200 GeV as measured at mid-rapidity by the PHENIX detector at RHIC. Precision resonance centroid and width values are extracted as a function of collision centrality. No significant variation from the PDG accepted values is observed. The transverse mass spectra are fitted with a linear exponential function for which the derived inverse slope parameter is seen to be constant as a function of centrality. These data are also fitted by a hydrodynamic model with the result that the freeze-out temperature and the expansion velocity values are consistent with the values previously derived from fitting single hadron inclusive data. As a function of transverse momentum the collisions scaled peripheral.to.central yield ratio RCP for the is comparable to that of pions rather than that of protons. This result lends support to theoretical models which distinguish between baryons and mesons instead of particle mass for explaining the anomalous proton yield.Comment: 326 authors, 24 pages text, 23 figures, 6 tables, RevTeX 4. To be submitted to Physical Review C as a regular article. Plain text data tables for the points plotted in figures for this and previous PHENIX publications are (or will be) publicly available at http://www.phenix.bnl.gov/papers.htm

Positive feedback and noise activate the stringent response regulator Rel in mycobacteria

Phenotypic heterogeneity in an isogenic, microbial population enables a subset of the population to persist under stress. In mycobacteria, stresses like nutrient and oxygen deprivation activate the stress response pathway involving the two-component system MprAB and the sigma factor, SigE. SigE in turn activates the expression of the stringent response regulator, rel. The enzyme polyphosphate kinase 1 (PPK1) regulates this pathway by synthesizing polyphosphate required for the activation of MprB. The precise manner in which only a subpopulation of bacterial cells develops persistence, remains unknown. Rel is required for mycobacterial persistence. Here we show that the distribution of rel expression levels in a growing population of mycobacteria is bimodal with two distinct peaks corresponding to low (L) and high (H) expression states, and further establish that a positive feedback loop involving the mprAB operon along with stochastic gene expression are responsible for the phenotypic heterogeneity. Combining single cell analysis by flow cytometry with theoretical modeling, we observe that during growth, noise-driven transitions take a subpopulation of cells from the L to the H state within a "window of opportunity" in time preceding the stationary phase. We find evidence of hysteresis in the expression of rel in response to changing concentrations of PPK1. Our results provide, for the first time, evidence that bistability and stochastic gene expression could be important for the development of "heterogeneity with an advantage" in mycobacteria.Comment: Accepted for publication in PLoS On