Wireless capsule endoscopy for the detection of small bowel diseases in HIV-1-infected patients

<p>Abstract</p> <p>Background and Aims</p> <p>In HIV-infected patients, manifestations of the disease are common in the gastrointestinal tract. The objective of our study was to evaluate the diagnostic yield of the Given^®Video Capsule System (Given Imaging, Yoqneam, Israel) in these patients.</p> <p>Methods</p> <p>After exclusion of GI-tract stenosis by anamnestic exploration, 49 patients were included into the study. Stratification: Group A (n = 19): HIV-positive, CD₄cell count < 200/μl, gastrointestinal symptoms present. Group B: HIV-positive, CD₄< 200/μl, without gastrointestinal symptoms (n = 19 Group) C: healthy volunteers (n = 11).</p> <p>Results</p> <p>In group A there was a total of 30 pathological findings, 15 of which with therapeutic implications. In group B, there was a total of 22 pathological findings, 5 relevant for therapy. In group C there was a total of 13 pathological findings, 3 with therapeutic relevance. In 89% (group A) vs. 26% (group B), pathological findings were detected distal the ligament of Treitz (p = 0.001). All capsules were recovered without complications after 12 to 96 h from the stool.</p> <p>Conclusion</p> <p>Wireless capsule endoscopy of the small intestine should be considered for HIV-infected patients with marked immunosuppression and gastrointestinal symptoms.</p

Vergleichende Untersuchungen über vier Thrombocyten-Zählverfahren

Anhand von fünf Anforderungen an ein optimales Thrombocyten-Zählverfahren wurden vier Methoden unter Routinearbeitsbedingungen vergleichend auf Präzision in der Serie, Ergebniskorrelationen der Methoden untereinander, praktische Durchführbarkeit und Zeitbedarf pro Bestimmung überprüft. Dabei zeigten sich mäßige Präzisionen bei dem modifizierten Zählverfahren nach Fonio und bei der Kammerzählung im Phasenkontrastmikroskop, gute bei Verwendung des Coulter Thrombocounter und des Technicon AutoCounter. Ausreichende Ergebniskorrelationen mit tolerabler Streuung der linearen Regression wurden allein bei Vergleich der beiden halb- bzw. vollmechanisierten Methoden untereinander gefunden. Die Problematik der Absolutmessung wird diskutiert. Auf Grund des Zeitbedarfs pro Bestimmung können für Notfall-Einzelbestimmungen das Phasenkontrastverfahren und der Coulter-Thrombocounter, für Serienbestimmungen in größerem Umfang der Technicon AutoCounter empfohlen werden.Peer Reviewe

Familial Defective Apolipoprotein B-100 in 12 Subjects and Their Kindred

Peer Reviewe

Schnellmethode zur Darstellung von Fettsäuremethylestern für die gaschromatographische Analyse

Peer Reviewe

Umesterung und Veresterung von lang- und kurzkettigen Fettsäuren in Kapillaren für die Gaschromatographie

Peer Reviewe

Rapid diagnosis of familial defective apolipoprotein B-100

Geisel J, Schleifenbaum T, Weisshaar B, Oette K. Rapid diagnosis of familial defective apolipoprotein B-100. European Journal of Clinical Chemistry and Clinical Biochemistry. 1991;29(6):395-399.A method is described for the rapid, economic and non-radioactive examination of DNA samples from hypercholesterolaemic patients for familial defective apolipoprotein B-100, using a modified polymerase chain reaction (PCR) protocol and restriction enzyme isoform genotyping. Because of the high prevalence of familial defective apolipoprotein B-100, which is estimated to be one in 500 in most screened general populations, interest is focussed on a simple method for detection of this point mutation. In our protocol a diagnostic restriction site is created by PCR, using a specifically designed partly mismatched primer. In the case of familial defective apolipoprotein B-100 the amplified DNA segment contains an additional ScaI site, whereas DNA amplified from the normal allele is resistant to ScaI digestion. A rapid differentiation between the two alleles is achieved by agarose gel electrophoresis of the ScaI-digested PCR product