Reproductive capacity of the red cusk-eel genypterus chilensis (Guichenot, 1848) in captivity

Indexación: Scopus.This work was supported by the FONDEF Project D06I 1024 “Development of technologies for the production of red cusk-eel fingerlings (Genypterus chilensis)”.Genypterus chilensis is a marine fish of high gastronomic demand, whose capture has declined in recent years due to overfishing. In the development of the farming technology, high mortalities were obtained during egg incubation. The objective of this study is to contribute to the knowledge of fecundity and eggs viability of G. chilensis in captivity. The spawns of G. chilensis were analyzed over a period of 2 years and 3 months. The total fecundity was estimated by counting the masses and eggs produced monthly throughout the period. The results confirm that G. chilensis is a partial spawner, since a female may more than two masses of eggs per day, due to a large amount of mass spawned per season (621 average). The total production of masses of the Farming Centre during the period was 2,290; of these, only 7% (166) corresponding to 15,330,517 eggs were incubated. Because of its high fecundity, G. chilensis produces numerous masses of eggs, of which only a small percentage reaches incubation, as well as it occurs in other marine fish. © 2018, Escuela de Ciencias del Mar. All rights reserved.https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0718-560X201800020048

Functional and Structural Investigation of Songbird Brain Projection Neurons with Shuttle and Find

Extended abstract of a paper presented at Microscopy and Microanalysis 2011 in Nashville, Tennessee, USA, August 7-August 11, 201

Screening for significant chronic liver disease by using three simple ultrasound parameters

Objectives Chronic liver diseases remain asymptomatic for many years. Consequently, patients are diagnosed belatedly, when cirrhosis is unmasked by lifethreatening complications. We aimed to identify simple ultrasound parameters for the screening of patients with unknown significant chronic liver disease. Methods Three hundred and twenty seven patients with chronic liver disease, liver biopsy, and ultrasound examination were included in the derivation set. 283 consecutive patients referred for ultrasound examination were included in the validation set; those selected according to the ultrasound parameters identified in the derivation set were then referred for specialized consultation including non-invasive fibrosis tests and ultimately liver biopsy if liver fibrosis was suspected. Results In the derivation set, three ultrasound parameters were independent predictors of severe fibrosis: liver surface irregularity, spleen length (>110 mm), and demodulation of hepatic veins. The association of ≥2 of the three above parameters provided 49.1% sensitivity and 86.9% specificity. In the validation set, at ≥2 of the three parameters were present in 23 (8%) of the patients. Among these patients, 8 had liver fibrosis (F ≥ 1), 5 had significant fibrosis (F  ≥2) and two cirrhosis. Conclusion The generalized search of three simple ultrasound signs in patients referred for abdominal ultrasound examination may be an easy way to detect those with silent but significant chronic liver disease

Use of Genuine Sources of Ergot Resistance in Species of the \u3ci\u3edilatata\u3c/i\u3e Group of \u3ci\u3ePaspalum\u3c/i\u3e

Paspalum dilatatum Poir. (dallisgrass) is an excellent C4 forage for summer production, highly productive with a wide distribution within warm-temperate regions. P. dilatatum is native to South America with special relevance for milk and meat production since its forage quality is superior to that of other C4 forage grasses and it shows tolerance to frost and water stress. In situations of temperate and warm temperate climate, the presence of these species plays a key role in the ecological stability of the system due to the complementarity of the growth cycles between winter and summer grasses. Claviceps paspali (ergot) is a fungus that mainly parasitizes Paspalum spp., generating a structure called sclerotia, in which indole-diterpenoid alkaloids are isolated. Despite the excellent forage characteristics of Paspalum species, there is a need for the generation of varieties able to overcome two major limitations that were identified early on in this species, such as ergot susceptibility and seed production. With this objective, selected genotypes were crossed between apomictic and sexual species of different ploidy using P. malacophyllum as a source of immunity. Immunity to ergot has been evaluated in the field for two years in different representative regions of Uruguay and the accessions that did not get sick were selected as pollen donors. Crosses made with P. flavescens showed a germination percentage ranging between 1.7 and 7.09, while in P. dilatatum var. Chirú the range was reduced between 0.99 and 1.25 according to the employed parents. The hybrid nature of the progeny is being verified by microsatellites and functional markers associated with immunity and DNA content estimated by flow cytometry. This work aims to generate the basis to transfer immunity from P. malacophyllum in selected genotypes of Paspalum species predominant in Pampa biome and to improve seed production

Intra-arterial injection of lipid nanocapsules loaded with rhenium-188 for the radiotherapy of hepatocellular carcinomas in rats

International audienc

Factors limiting complete tumor ablation by radiofrequency ablation

The purpose of this study was to determine radiological or physical factors to predict the risk of residual mass or local recurrence of primary and secondary hepatic tumors treated by radiofrequency ablation (RFA). Eighty-two patients, with 146 lesions (80 hepatocellular carcinomas, 66 metastases), were treated by RFA. Morphological parameters of the lesions included size, location, number, ultrasound echogenicity, computed tomography density, and magnetic resonance signal intensity were obtained before and after treatment. Parameters of the generator were recorded during radiofrequency application. The recurrence-free group was statistically compared to the recurrence and residual mass groups on all these parameters. Twenty residual masses were detected. Twenty-nine lesions recurred after a mean follow-up of 18 months. Size was a predictive parameter. Patients\u27 sex and age and the echogenicity and density of lesions were significantly different for the recurrence and residual mass groups compared to the recurrence-free group (p < 0.05). The presence of an enhanced ring on the magnetic resonance control was more frequent in the recurrence and residual mass groups. In the group of patients with residual lesions, analysis of physical parameters showed a significant increase (p < 0.05) in the time necessary for the temperature to rise. In conclusion, this study confirms risk factors of recurrence such as the size of the tumor and emphasizes other factors such as a posttreatment enhanced ring and an increase in the time necessary for the rise in temperature. These factors should be taken into consideration when performing RFA and during follow-up

A retrofit variable-rate control system for pressurized slurry tankers

n the last several decades, livestock effluent management practices and field slurry spreading operations have received increasing attention due to their economic and environmental implications. In this study, a variable-rate control system for pressurized slurry tankers was developed according a retrofit approach (i.e., as a self-standing module that can be adapted and mounted on existing slurry tanker equipment). The system provides farmers with a useful tool for achieving compliance with environmental protection regulations and for developing good practices for livestock and nitrogen management. This system is suitable for new and used pressurized tanker equipment. For field-testing purposes, this system was mounted on a double-axis 10 m3 slurry tanker equipped with a crawling nozzle distribution unit. Field experiments were conducted at two typical forward speeds (2 and 3 km h-1) and three different nitrogen application rates (170, 250, and 340 kg ha-1). Based on the experimental results, the system was generally capable of limiting the differences between the nominal and measured application rates to less than 9%. In addition, the data analysis indicated that the slurry spreading was not significantly affected by the forward speed of the tanker. The uniformity of nitrogen spreading was evaluated according to the UNI EN 13406:2002 standard. The results showed that the transverse field distribution was uniform throughout the working width of the machine and at all tested operating conditions, with maximum deviations that were limited to less than 15%

Combination of blood tests for significant fibrosis and cirrhosis improves the assessment of liver-prognosis in chronic hepatitis C

BACKGROUND: Recent longitudinal studies have emphasised the prognostic value of noninvasive tests of liver fibrosis and cross-sectional studies have shown their combination significantly improves diagnostic accuracy. AIM: To compare the prognostic accuracy of six blood fibrosis tests and liver biopsy, and evaluate if test combination improves the liver-prognosis assessment in chronic hepatitis C (CHC). METHODS: A total of 373 patients with compensated CHC, liver biopsy (Metavir F) and blood tests targeting fibrosis (APRI, FIB4, Fibrotest, Hepascore, FibroMeter) or cirrhosis (CirrhoMeter) were included. Significant liver-related events (SLRE) and liver-related deaths were recorded during follow-up (started the day of biopsy). RESULTS: During the median follow-up of 9.5 years (3508 person-years), 47 patients had a SLRE and 23 patients died from liver-related causes. For the prediction of first SLRE, most blood tests allowed higher prognostication than Metavir F [Harrell C-index: 0.811 (95% CI: 0.751-0.868)] with a significant increase for FIB4: 0.879 [0.832-0.919] (P = 0.002), FibroMeter: 0.870 [0.812-0.922] (P = 0.005) and APRI: 0.861 [0.813-0.902] (P = 0.039). Multivariate analysis identified FibroMeter, CirrhoMeter and sustained viral response as independent predictors of first SLRE. CirrhoMeter was the only independent predictor of liver-related death. The combination of FibroMeter and CirrhoMeter classifications into a new FM/CM classification improved the liver-prognosis assessment compared to Metavir F staging or single tests by identifying five subgroups of patients with significantly different prognoses. CONCLUSIONS: Some blood fibrosis tests are more accurate than liver biopsy for determining liver prognosis in CHC. A new combination of two complementary blood tests, one targeted for fibrosis and the other for cirrhosis, optimises assessment of liver-prognosis

Proposal to estimate the engine oil consumption in agricultural tractors

Lubrication plays a crucial role in a tractor engines' efficiency and durability. Without suitable lubrication, excessive friction will significantly reduce an engine's power, and high-intensity wear will damage the moving parts in a short period of time. A set of 178 agricultural tractor models from 20 different international manufacturers located in Europe, North America and Asia was used in this study. The tractor models were produced between 2000 and 2015 with rated engine power ranging from 30 to 428.8 kW. Rated engine power, crankcase oil capacity, and oil change intervals were derived from official test reports. Engine oil consumption was calculated using the method described in ASABE Standard D497.7, clause 3.4. A linear relationship between rated engine power and hourly oil consumption rate was confirmed, but the regression coefficients deviated from current values in the ASABE Standard. These results indicate that ASABE equation coefficients should be updated to more accurately estimate engine oil consumption for use in technical/economical evaluations and in the analysis of operating costs of new tractor models