72 research outputs found

    Study of long term atmospheric trace contaminant monitoring and control Final report

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    Environmental control and life support subsystem for monitoring and controlling trace contaminants in spacecraft cabin atmospher

    Differential Distribution of Ca2+-Activated K+ Channel Splice Variants among Hair Cells along the Tonotopic Axis of the Chick Cochlea

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    AbstractSummary We have cloned from the receptor epithelium of the chick cochlea a family of alternatively spliced cDNAs derived from cslo, which encodes a Ca2+-activated K+ channel like those shown to help determine the resonant frequency of electrically tuned hair cells. Our results from PCRs using template RNAs from both tonotopically subdivided receptor epithelia and single hair cells demonstrate differential exon usage along the frequency axis of the epithelium at multiple splice sites in cslo. We also show that single hair cells express more than one splice variant at a given splice site. Since channel isoforms encoded by differentially spliced slo transcripts in other species are functionally heterogeneous, these data suggest that differential processing of slo transcripts may account, at least in part, for the systematic variation in hair-cell membrane properties along the frequency axis of electrically tuned auditory receptor epithelia

    Plant damage in urban agroecosystems varies with local and landscape factors

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    Biotic and abiotic factors at local to landscape scales influence insect pest and disease dynamics in agricultural systems. However, relative to studies focused on the importance of these drivers of crop plant damage in rural agricultural systems, few studies investigate plant damage from herbivore insects and plant diseases in urban agroecosystems, and consequently, most urban farmers lack knowledge on crop protection tactics. Here we use three common crop species within urban agroecosystems (community gardens) distributed across an urban landscape as a model system to ask how local, landscape, and microclimate factors relate to herbivore and disease plant damage. We hypothesized that plant damage would be lower in gardens with greater local vegetation complexity, landscapeā€scale complexity, and less variable temperatures, but that the importance of factors is speciesā€ and damageā€specific. By measuring Brassica, cucurbit, and tomato insect pest and disease damage across the growing season, we confirmed that the importance of factors varies with crop species and by damage type. Both local complexity factors (e.g., number of trees and shrubs) and landscape complexity (percent natural cover in the landscape) relate to lower incidence of herbivore and disease damage on some crops, supporting our prediction that habitat heterogeneity at both local and landscape scales lowers plant damage. Greater temperature variability related to higher disease damage on tomatoes linking microclimate factors to disease prevalence. Yet, local complexity factors also related to higher incidence of plant damage for other crop species, indicating variable speciesā€level impacts of local management factors on plant damage. By measuring the abundance of fungusā€feeding lady beetles (Psyllobora) on cucurbits, we confirmed a strong association between natural enemies and powdery mildew. We provide a case study on how changes in local to landscapeā€scale factors relate to plant damage in urban agroecosystems and suggest how urban farmers and gardeners can apply this ecological knowledge to improve sustainable urban food production.TU Berlin, Open-Access-Mittel - 202

    Green and animal manure use in organic field crop systems

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    Dual-use cover/green manure (CGM) crops and animal manure are used to supply nitrogen (N) and phosphorus (P) to organically grown field crops. A comprehensive review of previous research was conducted to identify how CGM crops and animal manure have been used to meet N and P needs of organic field crops, and to identify knowledge gaps to direct future research efforts. Results indicate that: (a) CGM crops are used to provide N to subsequent cash crops in rotations; (b) CGM-supplied N generally can meet field crop needs in warm, humid regions but is insufficient for organic grain crops grown in cool and sub-humid regions; (c) adoption of conservation tillage practices can create or exacerbate N deficiencies; (d) excess N and P can result where animal manures are accessible if application rates are not carefully managed; and (e) integrating animal grazing into organic field crop systems has potential benefits but is generally not practiced. Work is needed to better understand the mechanisms governing the release of N by CGM crops to subsequent cash crops, and the legacy effects of animal manure applications in cool and sub-humid regions. The benefits and synergies that can occur by combining targeted animal grazing and CGMs on soil N, P, and other nutrients should be investigated. Improved communication and networking among researchers can aid efforts to solve soil fertility challenges faced by organic farmers when growing field crops in North America and elsewhere
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