The Proximate Composition and Sensory Evaluation of the Flours of Breadfruit (artocarpus altilis), Benth Seed (adenopus breviflorus) and their Composite Bread.

The proximate composition and sensory evaluation of breadfruit (Artocarpus altilis), benth seed (Adenopus breviflorus) and wheat flour (Triticum aestivum) were analysed. The proximate composition of the seed flour revealed that breadfruit flour had the lowest protein content (6.35%) while benth seed flour had the highest protein content of 28.37%. The percentage ash and crude fibre were high in breadfruit and benth seed when compared with wheat flour. The highest value for crude fat was recorded in benth seed flour (47.60%). However, the protein and fat content of the bread produced increased with increase in the level of substitution of the non-wheat flour. The sensory evaluation also revealed that wheat flour can be substituted with breadfruit or benth seed flour up to 20 % level without any significant difference in taste, appearance and colour of the resulting composite bread and that of the commercially available bread but their flavours and textures were quite different at all levels of substitution. Keywords:  Proximate composition, Sensory evaluation

Prevalence of intestinal protozoan parasites in stray and domicile dogs in Ilorin, North Central, Nigeria

Uncontrolled population of stray and domicile dogs with intestinal protozoan in close proximity to increasing densities of human population in urban environments is a common fact in developing countries, in conjunction with the lack of veterinary attention and zoonotic awareness, increases the risks of disease transmission. We examined using standard method 108 stool specimens of stray and domicile dogs collected randomly from Ilorin, state capital of Kwara State, Nigeria for intestinal protozoan. The prevalence of intestinal parasites from both stray and domicile dogs was 64(59.3%). Three parasites were encountered; Anchylostoma sp, Cryptosporidium sp and Isospora sp. Female dogs were more infected (48.9%) than male dogs (11.1%) (P<0.001) while the stray dogs had more intestinal parasitic infection (68.3%) than domicile dogs (46.7). More of Cryptosporidium sp was observed among strayed dogs (36.5%). Double infection was highest in stray dogs (12.7%) than in domicile dogs (4.4%) but not statistically significant (p=0.051). Consistent sanitary education must be included in public health government actions as a first step for the control of intestinal parasites in dogs and good sanitation and hygiene should be encouraged minimize environmental contamination and contact with the infectious oocysts that may be shed by dogs.Keywords: Stray dog, domicile dog, zoonoses, Crysptosporidium, Isospora

Evaluation and comparison of the Amino acid composition of three varieties of Lagerena siceraria melon seed flours

Amino acids of three varieties of Lageneria siceraria seed flours were determined using standard methods of analysis. The three varieties are rich in essential amino acids needed for human health and growth, with a mean of 53.15%. Sulphur amino acid and aromatic amino acid              of the seeds ranged from 17.06 mg/g protein to 24.10 mg/g protein and from 80.22mg/g protein to 89.00 mg/g protein respectively. The varieties of these melon seed flours are closely related; however, there are significant differences (P?0.05) in Cystine, showing that the varieties have some genetic differences. Key Words: Lageneria siceraria seed flours, Essential amino acid, Genetics, Aginine,  Cystine

Autism spectrum disorder in a community-based sample with neurodevelopmental problems in Lagos, Nigeria

Autism Spectrum Disorder (ASD) is a globally prevalent neurodevelopmental disorder for which early diagnosis and intervention is the mainstay of management. In the African continent, limited data is available regarding the non-clinic based samples. Lack of information available to caregivers and inadequate skilled manpower often limit early detection and access to the few available though under resourced services in the community. Community based screening can be an important drive to create awareness and improve information dissemination regarding services available for those living with this disorder. This is a descriptive cross-sectional study utilizing data obtained from participants of a community-based autism screening exercise. The surveillance exercise was part of the annual Orange Ribbon initiative for autism awareness and screening held in 2014. Data was obtained from 85 participants involved in the Autism Surveillance screening exercise within the Lagos community. Community public service radio announcements state wide and word of mouth were used to invite and enroll eligible participants to the screening and consultation exercise. A second stage screening and a brief sociodemographic questionnaire followed by a third stage clinical interview and evaluation using the Diagnostic and Statistical Manual of Mental Disorders - 5 Edition (DSM 5) were used. Appropriate consultation and referrals to services in the community were given. Participants had a mean age of 7.53 years (SD 4.35). Twenty-nine (34.5%) met the diagnosis of ASD. Other diagnosis included attention deficit hyperactivity disorder (ADHD), language and speech disorder, intellectual disability (8.3%) and learning disorders (9.5%). Main health concerns to caregivers were poor language development in all (100%), of which 11 (40.7%) were non-verbal; gaze avoidance was seen in 14 (48.3%) and challenging behavior in 12 (42.9%). Comorbidities included seizure disorders (3.4%) and ADHD (6.9%). Persons with autism had history of ASD behavior more often when compared to the other neurodevelopmental disorders and these findings were statistically significant. Referrals were given to caregivers to engage in services within the community. As seen in this study, community understanding of ASD is poor in such locations, in which many persons with other neurodevelopmental disorders are often presented as having autism. Caregivers in the study location are distressed by many symptoms associated with autism and their comorbid conditions. Currently there is an evident role for frequent large scale community based screening and autism awareness exercises possibly using inter-sectoral collaboration as a strategy.Yewande O. Oshodi, Andrew T. Olagunju, Motunrayo. A. Oyelohunnu, Elizabeth A. Campbell, Charles S. Umeh, Olatunji F. Aina, Wellington Oyibo, Folusho E.A. Lesi, Joseph D. Adeyem

Cadmium (II) Adsorption from Aqueous Solutions Using Onion Skins

Staff PublicationThe potential of onion skins for removal of aqueous Cd(II) was investigated. Onion skin powder was chemically modified using thioglycolic acid to develop a suitable, low-cost, and efficient adsorbent for the removal of Cd(II) from aqueous solutions. Influences of temperature, contact time, initial concentration of Cd(II), adsorbent dosage, and pH on the removal of Cd(II) were probed. Optimal adsorption conditions were found at pH 5 and 4, and at 60- and 30-min equilibrium time for the modified and native onion skins, respectively. The equilibrium process was well described by the Freundlich isotherm model. The maximum Cd(II) adsorption capacities, from the Langmuir model, are 17.86 mg/g (modified) and 21.28 mg/g (native). The adsorption process followed the mechanism of physisorption. Pseudo second-order rate equation fitted the kinetic data better than the pseudo first-order rate equation for the two adsorbents. Thermodynamic parameters, such as standard free energy change (ΔG°), standard enthalpy change (ΔH°), and standard entropy change (ΔS°), were calculated for adsorption experimental studies. The results showed that the adsorption of Cd(II) on native/unmodified and modified onion skins was a feasible process and exothermic under the studied conditions. The Cd(II) adsorbed was efficiently desorbed from adsorbent using 0.3 M HCl

Sequestration of Aqueous Lead(II) Using Modified and Unmodified Red Onion Skin

Staff PublicationThe efficacy of onion skins, both unmodified and chemically modified with thioglycolic acid, was investigated as alternative low-cost adsorbents for the sequestration of aqueous lead(II) ions from aqueous solution. The adsorbents were characterised using Fourier transform infrared spectroscopy and scanning electron microscopy – energy dispersive X-ray spectroscopy. Adsorption experiments were performed using batch sorption processes. The effects of contact time, pH, initial Pb(II) concentration, adsorbent dose, and temperature were investigated. Optimum sorption conditions were found at pH 4 and a 150 min equilibrium time for the modified onion skin and unmodified onion skin. The Langmuir, Freundlich, DubininRadushkevich and Temkin models were used to characterize the equilibrium experimental results. The equilibrium process was best described by the Freundlich isotherm. The maximum adsorption capacities of 4.878 and 6.173 mg/g were obtained for modified and unmodified adsorbents, respectively, using the Langmuir model. Kinetic studies indicated that the sorption of Pb(II) ions followed a pseudo-second-order model. Thermodynamic parameters such as standard enthalpy change (ΔH°), entropy change (ΔS°), and free energy change (ΔG°) were evaluated from the sorption experimental measurements. The results showed that the sorption process of Pb(II) ions on unmodified and modified onion skins was feasible and exothermic under the conditions used in this study. The sorption process followed the mechanism of physisorption

Serum Metabolomic Profiling Identifies Key Metabolic Signatures Associated With Pathogenesis of Alcoholic Liver Disease in Humans

Alcoholic liver disease (ALD) develops in a subset of heavy drinkers (HDs). The goals of our study were to (1) characterize the global serum metabolomic changes in well-characterized cohorts of controls (Cs), HDs, and those with alcoholic cirrhosis (AC); (2) identify metabolomic signatures as potential diagnostic markers, and (3) determine the trajectory of serum metabolites in response to alcohol abstinence. Serum metabolic profiling was performed in 22 Cs, 147 HDs, and 33 patients with AC using ultraperformance liquid chromatography-tandem mass spectrometry. Hepatic gene expression was conducted in Cs (n = 16) and those with AC (n = 32). We found progressive changes in the quantities of metabolites from heavy drinking to AC. Taurine-conjugated bile acids (taurocholic acid [TCA], 127-fold; taurochenodeoxycholic acid [TCDCA], 131-fold; and tauroursodeoxycholic acid, 56-fold) showed more striking elevations than glycine-conjugated forms (glycocholic acid [GCA], 22-fold; glycochenodeoxycholic acid [GCDCA], 22-fold; and glycoursodeoxycholic acid [GUDCA], 11-fold). This was associated with increased liver cytochrome P450, family 7, subfamily B, member 1 and taurine content (more substrates); the latter was due to dysregulation of homocysteine metabolism. Increased levels of GCDCA, TCDCA, GCA, and TCA positively correlated with disease progression from Child-Pugh A to C and Model for End-Stage Liver Disease scores, whereas GCDCA, GCA, and GUDCA were better predictors of alcohol abstinence. The levels of glucagon-like peptide 1 (GLP-1) and fibroblast growth factor (FGF) 21 but not FGF19 were increased in HDs, and all three were further increased in those with AC. Conclusion: Serum taurine/glycine-conjugated bile acids could serve as noninvasive markers to predict the severity of AC, whereas GLP-1 and FGF21 may indicate a progression from heavy drinking to AC

Impact of Mobile Technologies on Cervical Cancer Screening Practices in Lagos, Nigeria (mHealth-Cervix): A Randomized Controlled Trial

Purpose We assessed the impact of mHealth on Pap test screening uptake and also determined the factors that affect screening uptake among women in Lagos, Nigeria. Materials and Methods A randomized controlled trial was carried out among women in two tertiary health institutions in Lagos, Nigeria, between July 2020 and March 2021. Participants were randomly assigned to either a text message (mHealth) intervention or usual care arm. The main study outcome was the uptake of Pap smear testing within 6 months of enrollment in the study. We tested the associations between two groups of continuous variables using the unpooled independent-sample t-test (normal distribution) and that of two groups of categorical variables with the chi-square (χ2) test. Using a multinomial logistic regression model, we adjusted for relevant sociodemographic and clinical predictors of uptake of Pap smear screening. Statistical significance was defined as P < .05. Results There was a significantly higher rate of uptake of Pap smear screening among women in the mHealth arm compared with those in the usual care arm (51.0% v 35.7%, P = .031). Following adjustment in the final multivariate model, level of income (odds ratio [OR] = 5.13, 95% CI, 1.55 to 16.95), awareness of Pap smear (OR = 16.26; 95% CI, 2.49 to 76.64), General Outpatient clinic attendance, and introduction of mHealth intervention during follow-up (OR = 4.36; 95% CI, 1.44 to 13.22) were the independent predictors of Pap smear uptake. Conclusion The use of mHealth technologies intervention via short-text message services is a feasible solution for cervical cancer prevention in low- and middle-income countries, and thus, the widespread use of mHealth services by health care providers and policymakers could contribute to the implementation of cervical cancer prevention services in Nigeria and in the settings of other low- and middle-income countries

Quantity of alcohol drinking positively correlates with serum levels of endotoxin and markers of monocyte activation

It is unknown if LPS (lipopolysaccharides) and markers of immune activation, soluble CD14 (sCD14) and CD163 (sCD163) are associated with the quantity of alcohol consumption. 148 subjects were enrolled (97 excessive drinkers (ED) and 51 controls). Time Line Follow-Back questionnaire was used to quantify the amount of alcohol consumed. Serum LPS, sCD14, and sCD163 were measured. Peripheral blood mononuclear cells (PBMCs) were also isolated. Compared to controls, ED had higher total drinks in the past 30 days, higher levels of LPS, sCD14 and sCD163. The levels of serum LPS, sCD14, and sCD163 were higher among ED with recent alcohol consumption (last drink <10 days before enrollment) compared to those without recent drinking. Similar bacterial genome copy numbers were detected in control and ED groups. We found that ethanol primed PBMCs for LPS-induced inflammatory responses. A positive correlation between serum LPS, sCD14, sCD163 and the quantity of alcohol drinking was observed after adjusting for covariates and that abstinence was associated with decline in the levels of LPS, sCD14 and sCd163. We found an increase in the levels of LPS and markers of monocyte activations in ED. Further studies are needed to determine whether these can be used as the biomarkers for excessive alcohol use

The Exposure of Workers at a Busy Road Node to PM2.5: Occupational Risk Characterisation and Mitigation Measures

The link between air pollution and health burden in urban areas has been well researched. This has led to a plethora of effective policy-induced monitoring and interventions in the global south. However, the implication of pollutant species like PM2.5 in low middle income countries (LMIC) still remains a concern. By adopting a positivist philosophy and deductive reasoning, this research addresses the question, to what extent can we deliver effective interventions to improve air quality at a building structure located at a busy road node in a LMIC? This study assessed the temporal variability of pollutants around the university environment to provide a novel comparative evaluation of occupational shift patterns and the use of facemasks as risk control interventions. The findings indicate that the concentration of PM2.5, which can be as high as 300% compared to the WHO reference, was exacerbated by episodic events. With a notable decay period of approximately one-week, adequate protection and/or avoidance of hotspots are required for at-risk individuals within a busy road node. The use of masks with 80% efficiency provides sufficient mitigation against exposure risks to elevated PM2.5 concentrations without occupational shift, and 50% efficiency with at least ‘2 h ON, 2 h OFF’ occupational shift scenario