Collecting genetic materials and isolating DNAs of grapevine (Vitis spp.) as naturally grown in Ganos Mountains

Ganos Dağları Trakya’nın güneyinde 40° 35’ ve 40° 52’ K ile 26° 58’ ve 27° 27’ D arasındayer almaktadır. Kuzeydoğu-Güneybatı yönünde uzanmaktadır. Tekir Dağları’nın 945 m rakımile en yüksek yeridir. 2014 yılı vejetasyon periyodunda yürütülen araştırmada örnekleme üçfarklı yöntem izlenerek yapılmıştır. Arkeolojik kazılar sonucunda eski yerleşimlerin olduğubilinen alanlar ve yakınlarında; köylerde yaşayan kişilerin belirttiği asmalardan ve asmabulunması olasılığı olan bölge ve dere yataklarının aranması şeklinde yapılmıştır. Örnekler40° 53’ K ile 27° 26’ D ve 40° 35’ K ile 27° 00’ D koordinatları arasından toplanmıştır.“Second Edition of the OIV Descriptor List for GrapeVarieties and Vitis Species” listesindeyer alan 29 tanımlama karakteri kullanılarak irdelenmiştir. Alınan yaprak ve sürgün ucuörnekleri fotoğraflanmış ve incelenen özellikler açısından değerlendirilmiştir. ÖrneklerinDNA’ları “Doyle & Doyle DNA Ekstrasyon Protokolü” kullanılarak izole edilmiştir. Eldeedilen DNA’lar buzdolabında muhafaza altına alınmıştır. Sonuç olarak Ganos Dağları’nınkuzey yamaçlarında yaklaşık 600 m, güney yamaçlarında ise 700-750 m rakımlardan sonraVitis vinifera ssp. silvestris ve Vitis vinifera ssp. sativa örneklerine rastlanmamıştır. Köy ileyerleşim yerlerine uzak ve yoğun ormanlık alanlarda (aşırı gölge) bulunan bazı derelerde deörnek bulunmadığı; genellikle su bulunan nemli alanlarda, etrafı açık, kayalık derelerdeyetiştiği görülmüştür. Bu asmaların arasından gelecekte ıslah çalışmalarında kullanılmak üzereyeni bireylerin eldesi ihtimali olduğundan vejetatif materyal alınarak in-vivo’da saklanmasınınuygun olacağı ön görülmüştür.Ganos Mountains, lied on north-east and south-west direction are located in the south of Trakya Region in 40° 35’ and 40° 52’ N ile 26° 58’ ve 27° 27’ E coordinates. Ganos Mountains are the highest point of Tekir Mountains with 945 m altitude. Research was conducted in the vegetation period in 2014; sampling survey carried out using three different methods. Searching grapevines in the well-known oldest settlement areas which were about archaeological excavation areas; also asking the villagers, and some districts and riverbeds. Grapevine samples are collected between 40° 53' N and 27° 26' E and 40° 35' N 27° 00' D coordinates. Samples were examined on 29 identification characters according to the “Second Edition of the OIV’s Descriptor List for Grape Varieties and Vitis Species” list. Grapevine leaves and shoot apex samples were photographed and evaluated. Samples DNAs content were isolated using the “Doyle & Doyle DNA Extraction Protocol”. DNAs were preserved in refrigerator. As a result, in the northern slopes of the Ganos Mountains about 600 m altitude, while the southern slopes above 700-750 m altitude; neither Vitis vinifera ssp. silvestris nor Vitis vinifera ssp. sativa grapevine samples were found. Far from the villages and settlements also densely forested areas (excessive shade) and streams there was no samples found; while moist areas with water, open areas and rocky creek was have samples. It is foreseen that; in order touse in the future breeding programs these vegetative materials are kept in-vivo conditions

The Effects of Extraction and Nonextraction Treatment on RME Applied Patients

Aim:The aim of this study was to compare the dentoalveolar, skeletal and soft tissue effects of upper two premolar extraction or nonextraction treatments after Rapid Maxillary Expansion (RME).Subjects and Methods:The sample of this study consisted of pre and posttreatment lateral cephalometric radiographs of 21 patients characterized by narrow maxilla and treated with or without extraction following RME. In our study, RME applied patients were divided into two groups as extraction and nonextraction. After adequate expansion and retention period, two upper first premolars were extracted in extraction group and no teeth were extracted in nonextraction group. Thereafter, fixed orthodontic treatment was started in two groups.Results:At the end of treatment when extraction and nonex-traction groups were compared, significant differences in A1 inclination to A-Po angle (p < 0.01), A1 to A-Po plane (p < 0.05) and B1 to A-Po plane distance (p < 0.05) representing the dentoalveolar incisor position were detected. Also statistically no significant differences were found between parameters related to lips or soft tissues. Our results do not support the idea that more retrusive profile occurs as a result of upper extraction treatment.Conclusions:In the light of these results, it is possible to say that there is no esthetic difference between the RME appliances applied patients treated by either upper premolar extraction or nonextraction treatment

The Effects of Bagging Applications on Maturation, Yield and Quality in cv. Italia Grape

Araştırmada Italia çeşidinde 2012 ve 2013 yıllarında üç farklıtorbalama uygulamasının (Mogul 19 g m-2, Mogul 30 g m-2 ve Tyvek)olgunluk, verim ve kalite üzerine etkileri incelenmiştir. Torbalamauygulamaları tane tutumu döneminde gerçekleştirilmiştir.Çalışmanın yürütüldüğü her iki yılda da Mogul tip torbalarda torbaiçi sıcaklıklarının dış koşullara göre daha yüksek olduğu, Tyvek tiptorbalarda ise torba içi sıcaklıkların dış koşullara yakın seyrettiğibelirlenmiştir. Torba içi nem değerleri tüm torbalarda dış koşullaragöre düşük bulunmuş, Mogul tip torbalarda bu değer Tyvek tiptorbalara göre daha düşük ortaya çıkmıştır. Araştırma bulgularınagöre tane yarılma direnci ölçümlerinde açıkta geliştirilen tanelerintorbalama uygulanmış tanelere göre daha sağlam olduğubelirlenmiştir. Özellikle Mogul tip torbaların ise olgunlaşmayıhızlandırıcı etki gösterdiği belirlenmiştir.In the study, the effects of three different bagging applications (Mogul 19 g m-2, Mogul 30 g m-2 and Tyvek) on the maturity, yield and quality of Italia grape cultivar were investigated in 2012 and 2013. Bagging applications were carried out during the berry set period. In both years of the study, it was determined that the temperatures in Mogul type bags were higher than the external conditions, while the temperatures in Tyvek type bags were close to the external conditions. Moisture values were found to be lower in all bags compared to external conditions, this value was lower in Mogul type bags compared to Tyvek type bags. According to the research findings, the berries in the control application were found to be more robust than the bagged ones in terms of resistance to berry crushing. Especially, Mogul type bags were determined to have an accelerating effect on maturation

Characteristics of food allergy in children: National multicenter study

Conference: Congress of the European-Academy-of-Allergy-and-Clinical-Immunology (EAACI) Location: Lisbon, PORTUGAL Date: JUN 01-05, 2019Background : Food allergies impose a significant burden on the life of the child and the family. In this study, to determine the demographic characteristics of food allergies, we investigated the characteristics of patients with food allergies in different regions of Pediatric Allergy- Immunology departments in Turkey. Method : Turkey ' s National Study of Allergy and Clinical Immunology Society has conducted a Study Group on Food Allergies. 25 centers participated in this multicenter, cross- sectional and descriptive study.European Academy of Allergy and Clinical Immunolog

Turkish based library automation programs and using in university libraries

Information bomm in all over the world and publication capacity which increases because of publication bomm, a natural outcome of information bomm, users’ needs changing parallel to developments in technology ensure widespread use of computers, which is the best profit of modern technology, in the libraries. In this way ; speed, easiness and output are obtained in all processes and duties. Computers are, certainly, the most important invention of our time. Computers which ensure speed to which human power cannot reach perfection, reliability and straightnees started to be used in the libraries in 1960s and the number of library carrying out the use of computers increases parallel with the increase of using fields day by day. The aim of this work is, firstly, to reveal the works which were done from the first day to this time and the situation for today; secondly; to examine library automation programs in Turkish foundation which are used in university libraries in Turkey and lastly, to establish whether existing automation programs have standards in literature, at the same time whether libraries which use these programs are satisfied with programs. At the end of this work, it is determined that there are four library automation programs which are used by university libraries in our country and that there are some problems and shortage in each of them and that users are satisfied with only two of these four programs. It is thought that lockness of an organization, follows the developments in library automation field and directs automation activities in the frame of these developments, influences negatively

The peripheral blood inflammatory patterns in the control levels of asthma in children

Objective: Asthma is the most common chronic inflammatory disease of childhood, but there are no useful and easily accessible laboratory tests routinely used in the diagnosis and follow-up of this disease in children. Therefore, this study aimed to investigate the roles of white blood cell (WBC) count, platelet count, mean platelet volume (MPV), and eosinophil percentage as full blood count inflammatory markers in evaluating the control level and follow-up of asthma in the pediatric age group. Methods: A retrospective review of patient records and files of 3,580 patients diagnosed with asthma at the University of Health Sciences in Ankara, Turkey was performed. Patients who met inclusion/exclusion criteria were divided into two groups based on the asthma control level: controlled and uncontrolled. Laboratory data were compared according to the asthma control levels, drug use status, and atopy status of the patients. Results: A total of 348 patients between 4 and 18 years of age, who were followed-up with the diagnosis of asthma, were included in this study. A significant difference was found between the controlled and uncontrolled groups of asthma patients in terms of the eosinophil percentage (mean +/- SD, respectively; 3.493 +/- 2.24; 4.992 +/- 3.43; p = .003). When patients were grouped according to their asthma control levels and atopy status, only the eosinophil percentages were different in the logistic regression analysis (odds ratio = 1.276, 95% confidence interval = 1.113-1.462). Conclusion: Our study showed that the percentage of eosinophils can be used as an asthma control parameter, but additional prospective studies would be desirable to confirm our results

Genetic diversity of Turkish lathyrus L. landraces using ISSR markers

Fifty-one Lathyrus sativus L. landraces and one L. clymenum L. landrace collected from Turkey and one L. sativus cultivar, Gürbüz, were evaluated with ISSR markers in this study to molecular characterization. Three ISSR primers were used and 45 DNA fragment were evaluated, of which 44 bands were polymorphic. The frequencies of scored bands ranged from 0.009 to 0.888 and averaged 0.363. The genotypes MLT04 and NEV02 had high similarity with 0.825 according to pairwise grouping. The furthest pairwise group was Gürbüz and MLT02 with 0.244. The nearest genotype to Gürbüz was CNR03 with 0.577. The pairwise genetic distance between L. clymenum and L. sativus ranged from 0.353 (accession NEV01) to 0.637 (accession DEN04) and pairwise genetic distance to the cultivar Gürbüz was 0.375. Assessment of genetic relationships among Lathyrus genotypes made two main groups. One of them covered only Gürbüz variety and the other covered 52 Lathyrus landrace. L. sativus and L. clymenum separated prominently in the second group