Silvipastoral systems on Amazon river floodplains: identification of multipurpose forest species based on traditional knowledge of riverside inhabitants.

Resumo

Aspectos moleculares da interação do fungo Sclerotinia sclerotiorum e plantas de feijoeiro (Phaseolus vulgaris L.).

O presente trabalho teve como objetivo identificar os genes potencialmente envolvidos no processo de infecção de S. sclerotiorum em plantas de feijoeiro, através do mapeamento de sequências diferencialmente expressas obtidos em bancos de subtração, por meio da técnica de SSH.CONAFE

Caracterização da expressão de inibidores de poligalacturonases (pgips) em resposta aos estresses biótico e abiótico em plantas de feijão comum (Phaseolus vulgaris L.).

O presente trabalho teve como objetivo, avaliar a expressão de genes inibidores de poligalacturonases (PvPgip) em plantas de feijão comum (Phaseolus vulgaris L.) em resposta aos estresses biótico e abiótico.CONAFE

Unravelling the non-classicality role in Gaussian heat engines

At the heart of quantum thermodynamics lies a fundamental question about what is genuine "quantum" in quantum heat engines. With the help of the P-representability expression of classicality, the degree of non-classicality of a bosonic thermal engine - a quantum heat engine consisting of a single bosonic mode as a working substance coupled to a cold thermal and a hot squeezed thermal bath - is quantified. For two specific protocols, i.e., a quantum Otto and a generalized cycle, we show that the non-classicality is a fundamental resource for genuinely quantum heat engines.Comment: 8 pages, 7 figure

Comparação de configurações para leitura de potássio em ICP-OES.

O objetivo do trabalho foi comparar diferentes configurações do ICP-OES na determinação da concentração de K, em amostras de tecido vegetal, buscando reduzir o tempo de leitura das amostras e, consequentemente, o custo operacional do equipamento

Mistura em tanque de boro e herbicidas em semeadura convencional de girassol.

Os objetivos deste experimento foi avaliar a resposta do girassol às aplicações de boro (B), isoladas ou em mistura com herbicidas, e o controle de plantas daninhas por meio de experimento conduzido na Embrapa Soja, Londrina-PR. Os tratamentos foram acetochlor (1,92 kg i.a. ha-1), oxyfluorfen (0,36 kg i.a. ha-1), sulfentrazone (0,35 kg i.a.ha-1), trifluralin (1,80 kg i.a. ha-1) e as testemunhas capinada e sem capina. Todos os tratamentos foram aplicados, isoladamente ou em mistura, com 2 kg ha-1 de B (Na2B4O7.10H20 ? bórax e H3BO3 ? ácido bórico). O tratamento mais eficiente foi acetochlor mais ácido bórico; essa combinação resultou em solução mais homogênea da calda de pulverização, quando comparada com os herbicidas mais bórax. O herbicida acetochlor aplicado isoladamente ou em mistura com as duas fontes de B foi eficiente no controle da trapoeraba (Commelina benghalensis), do picão-preto (Bidens pilosa) e da corda-de-viola (Ipomoea grandifolia). Os herbicidas oxyfluorfen e sulfentrazone, aplicados isoladamente ou em misturas com as duas fontes de B, foram eficientes no controle do amendoim-bravo (Euphorbia heterophylla) e da corda-de-viola, respectivamente. É viável a aplicação de boro juntamente com os herbicidas testados nesta pesquisa em mistura em tanque, evitando a deficiência desse micronutriente e controlando as plantas daninhas na cultura do girassol

Systematic evaluation of software product line architectures

The architecture of a software product line is one of its most important artifacts as it represents an abstraction of the products that can be generated. It is crucial to evaluate the quality attributes of a product line architecture in order to: increase the productivity of the product line process and the quality of the products; provide a means to understand the potential behavior of the products and, consequently, decrease their time to market; and, improve the handling of the product line variability. The evaluation of product line architecture can serve as a basis to analyze the managerial and economical values of a product line for software managers and architects. Most of the current research on the evaluation of product line architecture does not take into account metrics directly obtained from UML models and their variabilities; the metrics used instead are difficult to be applied in general and to be used for quantitative analysis. This paper presents a Systematic Evaluation Method for UML-based Software Product Line Architecture, the SystEM-PLA. SystEM-PLA differs from current research as it provides stakeholders with a means to: (i) estimate and analyze potential products; (ii) use predefined basic UML-based metrics to compose quality attribute metrics; (iii) perform feasibility and trade-off analysis of a product line architecture with respect to its quality attributes; and, (iv) make the evaluation of product line architecture more flexible. An example using the SEI’s Arcade Game Maker (AGM) product line is presented as a proof of concept, illustrating SystEM-PLA activities. Metrics for complexity and extensibility quality attributes are defined and used to perform a trade-off analysis

Characterization of the dry bean polygalacturonase-inhibiting protein (PGIP) gene family during Sclerotinia sclerotiorum (Sclerotiniaceae) infection.

Polygalacturonase-inhibiting proteins are leucine-rich repeat proteins that inhibit fungal endopolygalacturonases. The interaction of polygalacturonase-inhibiting protein with endopolygalacturonases limits the destructive potential of endopolygalacturonases and may trigger plant defense responses induced by oligogalacturonides. We examined the expression of fungal pg and plant Pvpgip genes in bean (Phaseolus vulgaris) stems infected with Sclerotinia sclerotiorum to determine whether any of them are associated with the infection process. Transcriptional analysis was carried out by means of semi-quantitative reverse transcription PCR or real-time PCR. The sspg1 gene was highly expressed during infection; sspg3 was regulated during the later phases of infection; sspg5 was more uniformly expressed during infection, whereas sspg6 was only weakly expressed. During the course of infection, Pvpgip1 transcripts were not detected at early stages, but they appeared 72 h post-inoculation. High levels of Pvpgip2 expression were observed during the initial phase of infection; the transcript peaked by 48 h post-inoculation and declined by 72 h post-inoculation. Pvpgip3 expression increased strongly at 96 h post-inoculation. Pvpgip4 was constantly present from 24 h post-inoculation until the end of the experiment. However, we detected higher levels of the Pvpgip4 transcript in the necrotic lesion area than in plants that had been mechanically wounded. Remarkably, only Pvpgip4 appeared to be moderately induced by mechanical wounding. These results provide evidence that endopolygalacturonases contribute to the infection process during host colonization by promoting the release of plant cell oligogalacturonides, which are powerful signaling molecules and may also activate plant defenses, such as polygalacturonase-inhibiting proteins