Genetic Distance Based On Ssr And Grain Yield Of Inter And Intrapopulational Maize Single Cross Hybrids

The objective of this work was to correlate the genetic distances between the progenitors obtained by microsatellite markers with the grain yield of inter and intrapopulational maize single cross hybrids. Three S 0 populations derived from commercial single cross hybrids were used to obtain 163 hybrids (110 interpopulational and 53 intrapopulational). The two best hybrids and two worst hybrids of each the inter- and intrapopulational crosses were selected and their progenitors maintained through self-pollination of the second ear of each S 0 plant, genotyped with 47 SSRs. The Modified Roger's Distance (MRD) between each pair of S 1 inbred lines, the number of alleles and the polymorphic information content (PIC) of each primer were estimated. The genetic distances between progenitors were correlated with the grain yield of the inter- and intrapopulational hybrids. The number of obtained alleles was 186, with a mean of 3.96 alleles. The PIC varied from 0.49 to 0.80, with a mean of 0.65. The mean genetic distance between all S 1 inbred lines was 0.75, varying from 0.40 to 0.89, indicating the existence of variability between the S 1 inbred lines. The correlation between MRD and grain yield was high and significant for the interpopulational crosses (r = 0.84, P ≤ 0.01) and low and not significant (r = 0.18, P ≥ 0.05) for intrapopulational crosses.5103/04/15507513Ajmone Marsan, P., Castiglioni, P., Fu Sari, F., Kuiper, M., Motto, M., Genetic diversity and its relationship to hybrid performance in maize as revealed by RFLP and AFLP markers (1998) Theor. Appl. Genet., 96, pp. 219-227Árcade, A., Faivre-Rampant, P., Le Guerroué, B., Paques, L.E., Prat, D., Heterozigosity and hybrid performance in larch (1996) Theor. Appl. 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Chemical and Sensorial Characterization of Tropical Syrah Wines Produced at Different Altitudes in Northeast of Brazil

Over the years, viticulture has expanded to new regions outside the temperate zones, such as Northeast Brazil, India, Thailand, Myanmar, Vietnam, Bangladesh and Venezuela, characterized by the production of tropical wines. It is important for the productive sector to comprehend the effects of grapevine interaction with the characteristics of each new region on wines composition. In this study, the composition of wines of Syrah from two regions with different altitudes in Northeast Brazil were analyzed by different methodologies to characterize chemical compounds as sugar, acids, minerals, phenolics (anthocyanins, flavonols, stilbenes and condensed tannins) and the sensory profile. The wines of the Bahia region (1100 m of altitude) obtained high concentrations for chemical parameters related to color, monomeric anthocyanins, stilbenes and monomeric and oligomeric tannins. Wines of the low altitude region, Pernambuco (350 m of altitude) were characterized by higher concentrations of flavonols (kaempferol, isorhamnetin, quercetin and rutin) and polymerized tannins. The chemical composition of wines from the two studied regions was influenced by altitude. A trend towards higher concentrations in most for phenolic compounds analyzed was observed in wines from the higher altitude region during the two years of study. Regarding the sensory profile, fruity, floral, herbaceous and empyreumatic attributes aromatic obtained highest scores in wines of the 350 m altitude region, the other attributes were dependent on the year of harvest

Processing and characterization of glass-ceramic foams belonging to the Li2O-ZrO2-Al2O3-SiO2 (LZSA) system produced by gelcasting

In this work the viscosimetry technique was used to evaluate the rheological characteristics of ceramic suspensions prepared with a LZSA (Li2O-ZrO2-SiO2-Al2O3) glass. From the rheological characterization it was possible to establish optimized conditions of solid fraction, dispersant, organic monomers and foaming agent for the production of glass-ceramic foams by gelcasting. The resulting foams were subjected to heattreatments at 200-500ºC/60 min, for degradation of organics and at 950ºC/60 min for sintering and crystallization. With the obtained porous ceramic bodies linear thermal shrinkage, apparent density and mechanical strength measurements as well as microstructural analysis were performed. The adaptation of the rheological characteristics of the LZSA parent glass powder with the gelcasting processing technique allowed the production of ceramic foams with high open and interconnected porosity (>90%) with good thermal stability and with mechanical strength suitable for the production of porous ceramics.Neste trabalho, a técnica de viscosimetria foi utilizada para avaliar as características reológicas de suspensões preparadas com precursor vitrocerâmico do sistema LZSA (Li2O-ZrO2-SiO2-Al2O3). A partir da caracterização reológica, foi possível estabelecer condições composicionais adequadas de fração de sólidos, dispersante e monômeros orgânicos. A quantidade de agente espumante para produção de espumas vitrocerâmicas por gelcasting foi determinada pela variação volumétrica após agitação. As espumas resultantes foram submetidas a tratamentos térmicos no intervalo de temperatura compreendido entre 200 e 500ºC/60 min, para degradação da matéria orgânica e a 950ºC/60 min para sinterização e cristalização. Com os corpos cerâmicos porosos foram realizadas medidas de retração térmica, densidade aparente, análise microestrutural e resistência mecânica. A adequação das características reológicas do precursor LZSA por gelcasting permitiu a produção de espumas com elevada porosidade (> 90%) aberta e interconectada com boa estabilidade térmica e com resistência mecânica compatível com cerâmicas [email protected]@[email protected]@emc.ufsc.brUniversidade Federal de Santa Catarina Departamento de Engenharia Mecânica Programa de Pós-Graduação em Ciência e Engenharia de MateriaisCentro Universitário Barriga Verde - UNIBAVEUniversidade Federal de São Paulo (UNIFESP) Departamento de Ciência e TecnologiaUNIFESP, Depto. de Ciência e TecnologiaSciEL

Zoneamento agroecológico para a região de Ribeirão Preto utilizando um sistema de informações geográficas.

Objetivando contribuir com a metodologia de identificação de potencialidades de uso das terras, este trabalho apresenta uma proposta de zoneamento agroecológico da quadrícula de Ribeirão Preto, SP, localizada entre as coordenadas de 21o00'S a 21o30'S e 47o30'W a 48o00'W, com base em características de solo, relevo e clima, utilizando-se de um Sistema de Informações Geográficas. Para caracterizar o regime térmico-hídrico da área utilizou-se os dados de temperatura do ar e de chuva, de 22 localidades, referentes ao período de 1967 a 1996. De acordo com os critérios adotados, quanto a capacidade de uso das terras, o zoneamento identificou seis unidades de utilização da área: agricultura (I); agricultura (II); agricultura (III); pecuária; agrossilvicultura e preservação. As principais conclusões referentes à área de estudo foram: o regime térmico-hídrico é praticamente homogêneo, a vocação dominante é para agricultura (I) representando aproximadamente 191.118 hectares, correspondentes a 66,3% da área e cerca de 82,5% das terras possuem vocação para agropecuária e 10,4% devem ser preservadas ou utilizadas seguindo técnicas conservacionistas

Design of a nanostructured mucoadhesive system containing curcumin for buccal application : from physicochemical to biological aspects

Mucoadhesive nanostructured systems comprising poloxamer 407 and Carbopol 974P® have already demonstrated good mucoadhesion, as well as improved mechanical and rheological properties. Curcumin displays excellent biological activity, mainly in oral squamous cancer; however, its physicochemical characteristics hinder its application. Therefore, the aim of this study was to develop nanostructured formulations containing curcumin for oral cancer therapy. The photophysical interactions between curcumin and the formulations were elucidated by incorporation kinetics and location studies. They revealed that the drug was quickly incorporated and located in the hydrophobic portion of nanometer-sized polymeric micelles. Moreover, the systems displayed plastic behavior with rheopexy characteristics at 37 °C, viscoelastic properties and a gelation temperature of 36 °C, which ensures increased retention after application in the oral cavity. The mucoadhesion results confirmed the previous findings with the nanostructured systems showing a residence time of 20 min in porcine oral mucosa under flow system conditions. Curcumin was released after 8 h and could permeate through the porcine oral mucosa. Cytotoxicity testing revealed that the formulations were selective to cancer cells over healthy cells. Therefore, these systems could improve the physicochemical characteristics of curcumin by providing improved release and permeation, while selectivity targeting cancer cells

Elastase-2, a tissue alternative pathway for angiotensin II generation, plays a role in circulatory sympathovagal balance in mice

In vitro and ex vivo experiments indicate that elastase-2 (ELA-2), a chymotrypsin-serine protease elastase family member 2A, is an alternative pathway for angiotensin II (Ang II) generation. However, the role played by ELA-2 in vivo is unclear. We examined ELA-2 knockout (ELA-2KO) mice compared to wild-type (WT) mice and determined whether ELA-2 played a role in hemodynamics [arterial pressure (AP) and heart rate (HR)], cardiocirculatory sympathovagal balance and baroreflex sensitivity. The variability of systolic arterial pressure (SAP) and pulse interval (PI) for evaluating autonomic modulation was examined for time and frequency domains (spectral analysis), whereas a symbolic analysis was also used to evaluate PI variability. In addition, baroreflex sensitivity was examined using the sequence method. Cardiac function was evaluated echocardiographically under anesthesia. The AP was normal whereas the HR was reduced in ELA-2KO mice (425 ± 17 vs. 512 ± 13 bpm from WT). SAP variability and baroreflex sensitivity were similar in both strains. The LF power from the PI spectrum (33.6 ± 5 vs. 51.8 ± 4.8 nu from WT) and the LF/HF ratio (0.60 ± 0.1 vs. 1.45 ± 0.3 from WT) were reduced, whereas the HF power was increased (66.4 ± 5 vs. 48.2 ± 4.8 nu from WT) in ELA-2KO mice, indicating a shift toward parasympathetic modulation of HR. Echocardiographic examination showed normal fractional shortening and an ejection fraction in ELA-2KO mice; however, the cardiac output, stroke volume, and ventricular size were reduced. These findings provide the first evidence that ELA-2 acts on the sympathovagal balance of the heart, as expressed by the reduced sympathetic modulation of HR in ELA-2KO mice

Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured

Os efeitos do α-tocoferol e da ternatina sobre morfologia, ativação e crescimento de folículos pré-antrais caprinos cultivados in vitro, por um ou cinco dias, foram avaliados. Os fragmentos ovarianos foram imediatamente fixados (controle não-cultivado) ou cultivados in vitro, por um ou cinco dias, em Meio Essencial Mínimo (MEM) com ou sem suplementação com α-tocoferol ou ternatina nas concentrações de 5, 10 ou 15M, formando os tratamentos MEM, TOC5, TOC10, TOC 15, TER5, TER10, TER15. O percentual de folículos pré-antrais normais no controle não-cultivado foi de 73,2%, depois de cinco dias de cultivo, houve redução desse percentual em todos os tratamentos, quando comparados com o controle não-cultivado (P<0,05). O cultivo por cinco dias aumentou a ativação folicular em todos os tratamentos (P<0,05). A análise ultra-estrutural não mostrou folículos pré-antrais íntegros após cinco dias de cultivo em meio contendo antioxidantes. Concluiu-se que o α -tocoferol e a ternatina podem promover a ativação folicular, no entanto a adição desses antioxidantes nas concentrações testadas reduziu a viabilidade folicular após o cultivo in vitro. ______________________________________________________________________________________________________________ ABSTRACTThe effects of α-tocopherol and ternatin on the morphology, activation, and growth of goat preantral follicles in vitro cultured, for one or five days, were evaluated. Ovarian fragments were immediately fixed (non-cultured control) or in vitro cultured for one or five days in Minimum Essential Medium (MEM) with or without α-tocopherol or ternatin supplementation, both at concentrations of 5, 10, or 15M, corresponding to the following treatments: MEM, TOC5, TOC10, TOC 15, TER5, TER10, and TER15. The percentages of morphologically normal preantral follicles in non-cultured ovarian tissue (control) was 73.2% and after five days of culture, there was a decrease on these percentages in all treatments (P<0.05) when compared with non-cultured control. Culture of ovarian cortex for five days increased the percentages of follicular activation in all treatments (P<0.05). Ultrastructural analysis did not confirm the integrity of caprine preantral follicles cultured for five days in medium containing antioxidants. This study demonstrated that α-tocopherol and ternatin can promote follicular activation; however, addition of these antioxidants in the tested concentrations reduced the follicular viability after in vitro culture

The Consistent Result of Cosmological Constant From Quantum Cosmology and Inflation with Born-Infeld Scalar Field

The Quantum cosmology with Born-Infeld(B-I) type scalar field is considered. In the extreme limits of small cosmological scale factor the wave function of the universe can also be obtained by applying the methods developed by Hartle-Hawking(H-H) and Vilenkin. H-H wave function predicts that most Probable cosmological constant $\Lambda$ equals to $\frac{1}{\eta}$($\frac{1}{2\eta}$ equals to the maximum of the kinetic energy of scalar field). It is different from the original results($\Lambda=0$) in cosmological constant obtained by Hartle-Hawking. The Vilenkin wave function predicts a nucleating unverse with largest possible cosmological constant and it is larger than $1/\eta$. The conclusions have been nicely to reconcile with cosmic inflation. We investigate the inflation model with B-I type scalar field, and find that $\eta$ depends on the amplitude of tensor perturbation $\delta_h$, with the form $\frac{1}{\eta}\simeq \frac{m^2}{12\pi[(\frac{9\delta_{\Phi}^2}{N \delta_h^2})^2-1]}.$ The vacuum energy in inflation epoch depends on the tensor-to-scalar ratio $\frac{\delta_h}{\delta_{\Phi}}$. The amplitude of the tensor perturbation ${\delta_{h}}$ can, in principle, be large enough to be discovered. However, it is only on the border of detectability in future experiments. If it has been observed in future, this is very interesting to determine the vacuum energy in inflation epoch.Comment: 12 pages, one figure, references added, accepted by European Physical Journal