FUNCTIONAL SPECIALIZATION OF DUPLICATED FLAVONOID BIOSYNTHESIS GENES IN WHEAT

Gene duplication followed by subfunctionalization and neofunctionalization is of a great evolutionary importance. In plant genomes, duplicated genes may result from either polyploidization (homoeologous genes) or segmental chromosome duplications (paralogous genes). In allohexaploid wheat Triticum aestivum L. (2n=6x=42, genome BBAADD), both homoeologous and paralogous copies were found for the regulatory gene Myc encoding MYC-like transcriptional factor in the biosynthesis of flavonoid pigments, anthocyanins, and for the structural gene F3h encoding one of the key enzymes of flavonoid biosynthesis, flavanone 3-hydroxylase. From the 5 copies (3 homoeologous and 2 paralogous) of the Myc gene found in T. aestivum, only one plays a regulatory role in anthocyanin biosynthesis, interacting complementary with another transcriptional factor (MYB-like) to confer purple pigmentation of grain pericarp in wheat. The role and functionality of the other 4 copies of the Myc gene remain unknown. From the 4 functional copies of the F3h gene in T. aestivum, three homoeologues have similar function. They are expressed in wheat organs colored with anthocyanins or in the endosperm, participating there in biosynthesis of uncolored flavonoid substances. The fourth copy (the B-genomic paralogue) is transcribed neither in wheat organs colored with anthocyanins nor in seeds, however, it’s expression has been noticed in roots of aluminium-stressed plants, where the three homoeologous copies are not active. Functional diversification of the duplicated flavonoid biosynthesis genes in wheat may be a reason for maintenance of the duplicated copies and preventing them from pseudogenization.The study was supported by RFBR (11-04-92707). We also thank Ms. Galina Generalova for technical assistance

Development of laboratory information management system

Results of the actual direction of work of Scientific Research Institute of HV on development of the program complex LIS/LIMS «Chemist - analysts» for automatization of activity of analytical laboratories are presented. Functions of the complex and methodological principles of its development are considered, comparison with foreign analogues is carried out. The model of the industrial analytical control with use of concepts of a life cycle of a laboratory, technique and test is described

Conductance oscillations and zero-bias anomaly in a single superconducting junction to a three-dimensional Bi2Te3Bi_2Te_3 topological insulator

We experimentally investigate Andreev transport through a single junction between an s-wave indium superconductor and a thick film of a three-dimensional $Bi_2Te_3$ topological insulator. We study $Bi_2Te_3$ samples with different bulk and surface characteristics, where the presence of a topological surface state is confirmed by direct ARPES measurements. All the junctions demonstrate Andreev transport within the superconducting gap. For junctions with transparent $In-Bi_2Te_3$ interfaces we find a number of nearly periodic conductance oscillations, which are accompanied by zero-bias conductance anomaly. Both effects disappear above the superconducting transition or for resistive junctions. We propose a consistent interpretation of both effects as originating from proximity-induced superconducting correlations within the $Bi_2Te_3$ topological surface state

Executive Summary: Young people, education, employment and ESOL

The report, ‘Young people, education, employment and ESOL’ reviewed 47 studies1 to examine how current ESOL provision2 meets the needs of young people aged 16-25 years who use English as an Additional Language (EAL)3, and who need time and support to develop their English language skills in order to progress in education, training and employment

Complementary methods for the prevention and treatment of stress-induced mastopathy

The article considers the relationship between stress and diseases of the female reproductive system. A method for the rehabilitation of patients with mastopathy resulting from psycho-emotional stress (stress) based on the use of complementary methods of prevention and medical rehabilitation is proposed

GaAs(111)A and B in hydrazine sulfide solutions : extreme polarity dependence of surface adsorption processes

Chemical bonds formed by hydrazine-sulfide treatment of GaAs(111) were studied by synchrotron photoemission spectroscopy. At the B surface, the top arsenic atoms are replaced by nitrogen atoms, while GaAs(111)A is covered by sulfur, also bonded to underlying gallium, despite the sulfide molar concentration being 103 times smaller than that of the hydrazine. This extreme dependence on surface polarity is explained by competitive adsorption processes of HS- and OH- anions and of hydrazine molecules, on Ga- adsorption sites, which have distinct configurations on the A and B surfaces

Вихрова динаміка сполучених течій

Group constructions of bluff bodies are widely used in bridge construction practice. The junction flows of such structures are characterized by considerable complexity, nonstationarity and instability. In the vicinity of bluff bodies, systems of horseshoe vortex structures, shear layers, separated regions, jet flows, wake vortices and vortex Karman’s streets are formed. The study of the features of the generation and evolution of vortex and jet flows, the mechanisms of interaction of these flows with streamlined surfaces requires considerable effort during numerical and physical modeling. The purpose of the work is to determine the features of vortex and jet flow in the region of junction of three-row pile grillage with a rigid flat surface. Experimental studies were carried out in laboratory conditions in a hydrodynamic channel, where the three-row group of cylinders was installed on the hydraulically smooth rigid surface. Visual investigations and measurements of the velocity field were carried out inside and around the three-row grillage. The features of the formation and evolution of vortex and jet flows inside and near the cylindrical group were established. Integral and spectral characteristics of the velocity fluctuation field were obtained. Pages of the article in the issue: 25 - 28 Language of the article: UkrainianГрупові конструкції погано обтічних тіл широко використовуються у мостобудівній практиці. Сполучені течії таких конструкцій характеризуються значною складністю, нестаціонарністю та нестійкістю. В околі погано обтічних тіл формуються системи підковоподібних вихрових структур, зсувні шари, відривні області, струменеві течії, слідні вихори та вихрові доріжки Кармана. Мета роботи – визначення особливостей вихрової та струменевої течії в області сполучення трирядного пального ростверку з жорсткою пласкою поверхнею. Проведені експериментальні дослідження в лабораторних умовах за допомогою візуалізації та вимірювання полів швидкості сполученої течії. Визначено механізм генерації та еволюції вихрових і струменевих течій. Отримані інтегральні та спектральні характеристики поля швидкості в околі передньої групи циліндрів трирядного ростверку