Opportunities of Digitalization of Economic Subjects in Educational Process

In the conditions of the accelerated development of scientific and technical progress, and, first of all, information and computer technologies in scales not only one concrete country, and all set of the developed countries which are in a stage of a post-industrial service state, creation of intercountry communications, the great value is gained by a position of the higher education. Thus, institutions of higher professional education should become so-called points of growth or locomotives of growth of the level of application of information and computer technologies, algorithms for solving economic and management problems based on the use of economic and mathematical methods and models that students master in the process of studying the relevant disciplines. The main objectives of training in other disciplines, and especially management and economic complex is the application, consolidation and increase of knowledge, skills of digital technologies in the preparation of course work, final qualifying work, during research work as students in bachelor’s and master’s degree, and in graduate school. The level of computer-digital technology proficiency affects not only the validity of management and economic decisions in the production and financial and economic activities of economic entities, both commercial and non-commercial, but also on the research activities not only in higher education, but also abroad, in the field of fundamental and applied research, development. In addition, the need to master digital technologies is increasingly covering the daily life of the population, not only large cities, but also any settlements. Therefore, the strategic direction of development of Russian socio-economic processes, which is declared by the Government and the President of the Russian Federation as a priority, is the transition to a digital economy or digitalization of all life. Therefore, the development of educational guidelines for the solution of financial and economic problems and their application in the educational process is an urgent task, the solution of which allows to improve the level of competence of graduates of higher education in matters of digitalization not only in socio-economic activities, but also to use this knowledge and competence in all spheres of activity — cultural, environmental, scientific, technical and others

Detection of DNA autoantibodies using a novel amperometric biosensor on the basis of platinum(II) complex with DNA | Opredelenie autoantitel k DNK s pomoshch'iu novogo amperometricheskogo biosensora na osnove kompleksa platiny(II) s DNK.

A biochemical biosensor was developed on the basis of a steady-state Hg-film electrode and either DNA molecules or antibodies to DNA immobilized in a cellulose nitrate film. This biosensor is designed to measure the concentration of DNA (or antibodies to DNA), and it can be used in the diagnosis of autoimmune diseases. Electric current of catalytic H2 evolution caused by complexing between DNA (antibodies to DNA) and Pt(II) was used as an analytical signal. The detection sensitivity threshold for DNA and antibodies to DNA was 10 and 0.075 microgram/ml, respectively

A New Amperometric Biosensor Based on Platinum(II) Complex with DNA for Detecting Autoantibodies to DNA

A biochemical biosensor was developed on the basis of a steady-state Hg-film electrode and either DNA molecules or antibodies to DNA immobilized in a cellulose nitrate film. This biosensor is designed to measure the concentration of DNA (or antibodies to DNA), and it can be used in the diagnosis of autoimmune diseases. Electric current of catalytic H2 evolution caused by complexing between DNA (antibodies to DNA) and Pt(II) was used as an analytical signal. The detection sensitivity threshold for DNA and antibodies to DNA was 10 and 0.075 μg/ml, respectively

表紙、執筆者紹介、奥付、裏表紙

A biochemical biosensor was developed on the basis of a steady-state Hg-film electrode and either DNA molecules or antibodies to DNA immobilized in a cellulose nitrate film. This biosensor is designed to measure the concentration of DNA (or antibodies to DNA), and it can be used in the diagnosis of autoimmune diseases. Electric current of catalytic H2 evolution caused by complexing between DNA (antibodies to DNA) and Pt (II) was used as an analytical signal. The detection sensitivity threshold for DNA and antibodies to DNA was 10 and 0.075 μg/ml, respectively

A new amperometric biosensor based on platinum(II) complex with DNA for detecting autoantibodies to DNA

A biochemical biosensor was developed on the basis of a steady-state Hg-film electrode and either DNA molecules or antibodies to DNA immobilized in a cellulose nitrate film. This biosensor is designed to measure the concentration of DNA (or antibodies to DNA), and it can be used in the diagnosis of autoimmune diseases. Electric current of catalytic H2 evolution caused by complexing between DNA (antibodies to DNA) and Pt (II) was used as an analytical signal. The detection sensitivity threshold for DNA and antibodies to DNA was 10 and 0.075 μg/ml, respectively

Voltammetric determination of the components of biospecific deoxyribonucleic acid (DNA)-DNA antibody interaction as their Pt(II) complexes

A biochemical sensor based on a stationary mercury film electrode and a cellulose nitrate film containing either DNA molecules or molecules of antibodies to DNA was developed to determine the concentration of DNA (antibodies to DNA) and to diagnose autoimmune diseases. The peak currents of catalytic hydrogen liberation upon the formation of Pt(II)-DNA (antibodies to DNA) complexes were used as the analytical signal. The detection limits were 0.075 and 10 μg/mL for antibodies and DNA, respectively. © 1998 MAEe Cyrillic signK Hayκa/Interperiodica Publishing

The role of non-commercial cyprinids in maintenance and spread of the opisthorchiasis focus in the middle Ob River basin (Tomsk region, Russia)

The study assessed the role of non-commercial cyprinid species in maintaining the opisthorchiasis focus in the middle Ob River basin, Tomsk region, Russia. The source of O. felineus infection for humans and carnivores is fish of the family Cyprinidae. This is the most numerous family, 14 species live in the middle Ob River basin, which includes 6 commercial species and 8 non-commercial species. This study aimed to investigate the current situation on infestation of non-commercial cyprinids with O. felineus metacercariae and their role in maintaining and spreading the natural focus of opisthorchiasis in the middle Ob River basin. We investigated 4 non-commercial species (tench, sunbleak, common bleak, gudgeon), which are highly abundant in water bodies. Tench, common bleak and gudgeon are objects of amateur fishing. These species are traditionally included in the diet of the local population. Opisthorchis felineus metacercariae were recorded in muscles of all the examined fish species. The identification of metacercariae was confirmed by morphological methods and PCR diagnostics. Tench and sunbleak are the main sources of opisthorchiasis infection in the floodplain lakes of the Ob River basin (the prevalence of tench infection is 89.3% and mean intensity of infection is 11.2 metacercariae per fish, the prevalence of sunbleak infection is 50.9% and the intensity of infection is 4.25 metacercariae per fish). The prevalence of infection in the introduced common bleak from the rivers of the middle Ob River basin is rapidly increasing from 2.4 (2016-2018) to 37.5% (2020-2021), and mean intensity of infection increased from 1 to 4.15. The epizootic state of water bodies in the middle Ob River basin remains unfavorable in relation to opisthorchiasis. Tench, common bleak and sunbleak, along with ide and dace, are the main source of infection for humans and animals, which is evidenced by high infection with Opisthorchis felineus metacercariae in these numerous fish species. They pose the greatest danger of infection of people and animals with opisthorchiasis. These species should be included in the campaign to avoid raw and poorly cooked fish in the diet. In addition, such species as roach, bream and sunbleak also pose the danger of infection with opisthorchiasis, but to a lesser extent

Оптимизация иммуносупрессивной терапии при выполнении третьей пересадки почки в раннем послеоперационном периоде. Клиническое наблюдение

The choice of immunosuppressive therapy is determined by the degree of sensitization to the histocompatibility gene complex on chromosome 6 (HLA). The risk of rejection in the early periods after surgery  increases for the patients with repeated kidney transplantation. Optimizing immunosuppressive therapy is the only  way to prolong the life of a patient with a terminal stage of chronic renal failure. имости на 6-й хромосоме (НLA). The analysis of a clinical case of a 47-year-old patient who was undergoing treatment at the N.V. Sklifosovsky Scientific Research Institute of Emergency Medicine after the third allotransplantation of a cadaveric kidney in 2016 was performed. The patient was diagnosed with chronic glomerulonephritis (IgA-nephropathy) chronic end-stage renal failure; in the early postoperative period, in addition to basic immunosuppression, anti-lymphocytic polyclonal antibodies were prescribed in combination with plasmapheresis sessions for the treatment and prevention of acute rejection crisis in the early postoperative period. For the first time, in order to prevent the development of an acute rejection crisis and minimize infectious complications of immunosuppressive therapy in the recipient after the third kidney transplant, plasmapheresis sessions were used using a plasmapheresis filter with a polymethylacrylate membrane in combination with a short course of polyclonal antibodies.Выбор иммуносупрессивной терапии определяется степенью сенсибилизации к  комплексу генов гистосовместимости на 6-й хромосоме (НLA). У пациентов при повторной  пересадке почки риск отторжения в ранние сроки после операции увеличивается. Оптимизация иммуносупрессивной терапии – единственный путь продления жизни пациента с хронической почечной недостаточностью в терминальной стадии. Проведен анализ  клинического случая пациента 47 лет после выполнения третьей аллотрансплантации  трупной почки в 2016 г., находившегося на лечении в НИИ СП им. Н.В. Склифосовского с диагнозом «хронический гломерулонефрит (IgA-нефропатия), хроническая почечная недостаточность, терминальная стадия». В раннем послеоперационном периоде помимо  базовой иммуносупрессии были назначены антилимфоцитарные поликлональные антитела в сочетании с сеансами плазмафереза для лечения и профилактики острого криза отторжения в ранние сроки после операции.Впервые с целью профилактики развития острого криза отторжения, минимизации  инфекционных осложнений иммуносупрессивной терапии у реципиента после третьей  пересадки почки были применены сеансы плазмафереза с использованием плазмофильтра с полиметокрилатной мембраной в сочетании с коротким курсом поликлональных антител

Вовлеченность полиморфных вариантов гена EPHX1 в формирование хронической патологии легких профессионального и непрофессионального генеза в популяции жителей Курской области

Summary. Recently, growing attention has been paid to the genetic predisposition to respiratory diseases. A major direction is searching candidate genes responsible for occurrence and course of the disease. This study investigated the involvement of epoxide hydrolase gene group in development of occupational respiratory diseases and nonoccupational chronic bronchitis.Резюме. В последнее время все больше внимания уделяется генетической предрасположенности к возникновению патологии бронхолегочной системы. Основными направлениями являются поиск и изучение генов-кандидатов, ответственных за возникновение и характер течения заболевания. В данном исследовании проводилось изучение вовлеченности гена группы эпоксидгидролаз в формирование профессиональной бронхолегочной патологии и хронического бронхита непрофессиональной этиологии

Biomarkers of Air-Blood Barrier Damage In COVID-19

The search for sensitive and specific markers enabling timely identification of patients with a life-threatening novel coronavirus infection (COVID-19) is important for a successful treatment.The aim of the study was to examine the association of molecular biomarkers of air-blood barrier damage, surfactant proteins SP-A and SP-D and Club cell protein CC16, with the outcome of patients with COVID-19.Materials and methods. A cohort of 109 patients diagnosed with COVID-19 was retrospectively divided into two groups. Group 1 comprised survivor patients discharged from the ICU (w=90). Group 2 included the patients who did not survive (w=19). Association of disease outcome and SP-A, SP-D, and CC16 levels in blood serum, clinical, and laboratory data were examined taking into account the day of illness at the time of biomaterial collection.Results. The non-survivors had higher SP-A (from days 1 to 10 of symptoms onset) and lower CC16 (from days 11 to 20 of symptoms onset) levels vs survivors discharged from ICU. No significant differences in SP-D levels between the groups were found.Conclusion. According to the study results, the surfactant protein SP-A and Club cell protein CC16 are associated with increased COVID-19 mortality