The Relationship Between Lifestyle and Eating Behaviors in College Students

A Research Methods Project supervised by Dr. Laura Wilson (Fall 2021)

Influence of the ratio of planktonic to benthic diatoms on lacustrine organic matter δ13C from Erlongwan maar lake, northeast China

Carbon isotope ratio (δ13Corg) values of organic matter in lake sediments are commonly used to reconstruct environmental change, but the factors which influence change are varied and complex. Here we report δ13C values for sediments from Erlongwan maar lake in northeast China. In this record, changes in δ13C cannot be explained by simple changes in aquatic productivity. Instead, values were likely influenced by differences in the ratio between planktonic and benthic algae, as indicated by the remains of diatoms. This is because the variation of δ13Corg in algae from different habitats is controlled by the thickness of the diffusive boundary layer, which is dependent on the turbulence of the water. Compared with benthic algae, which grow in relatively still water, pelagic algae are exposed to greater water movement. This is known to dramatically reduce the thickness of the boundary layer and was found to cause even more severe δ13C depletion. In Erlongwan maar lake, low values were linked to the dominance of planktonic diatoms during the period commonly known as the Medieval Warm Period. Values gradually increased with the onset of the Little Ice Age, which we interpret as being driven by an increase in the proportion of benthic taxa, due to effect of the colder climate. The increase in planktonic diatoms at the end of the Little Ice Age, linked to higher temperature and a reduction in ice cover, resulted in a further decline in δ13Corg

Biallelic PI4KA variants cause a novel neurodevelopmental syndrome with hypomyelinating leukodystrophy

Phosphoinositides are lipids that play a critical role in processes such as cellular signalling, ion channel activity and membrane trafficking. When mutated, several genes that encode proteins that participate in the metabolism of these lipids give rise to neurological or developmental phenotypes. PI4KA is a phosphoinositide kinase that is highly expressed in the brain and is essential for life. Here we used whole exome or genome sequencing to identify 10 unrelated patients harbouring biallelic variants in PI4KA that caused a spectrum of conditions ranging from severe global neurodevelopmental delay with hypomyelination and developmental brain abnormalities to pure spastic paraplegia. Some patients presented immunological deficits or genito-urinary abnormalities. Functional analyses by western blotting and immunofluorescence showed decreased PI4KA levels in the patients' fibroblasts. Immunofluorescence and targeted lipidomics indicated that PI4KA activity was diminished in fibroblasts and peripheral blood mononuclear cells. In conclusion, we report a novel severe metabolic disorder caused by PI4KA malfunction, highlighting the importance of phosphoinositide signalling in human brain development and the myelin sheath

Reimagining Housing Options for the Sisters Elementary School Site

56 pagesUniversity of Oregon Architecture students proposed site designs and adaptive reuse ideas for the existing Sisters Elementary School and outdoor recreational uses and affordable housing. Student housing proposals fell into four main categories: 1) Housing on top of the school 2) Housing on the west side of the site 3) Housing in groups of clusters 4) Housing as larger volumes Students worked together and independently to improve the quality of life and community in Sisters, Oregon, through design. Students focused on creating spaces for future residents that would serve a range of family sizes and types. Students also proposed outdoor spaces that are designed to serve the community in a variety of ways, mostly through recreational activities such as sports. Proposed designs considered the future Highway 20 roundabout that will be built adjacent to the site, which will slow traffic down as it travels through the central part of Sisters. Students also proposed site layouts that will better accommodate pedestrians and bicyclists as they travel through and around the site. To formulate their designs, students started with case studies, diagrams, and process models. They also completed a detailed site analysis, which included a field trip to the site and the city of Sisters. This helped the students visualize and understand the site, the community, and the community’s needs. Students also researched relevant demographics within Sisters, Oregon, including the school district since their project focus included adaptive reuse of the current elementary school and affordable housing for existing and incoming teachers. Every student designed distinct details within their designs, such as splash pads, a community amphitheater, gardens, and unique connections from the proposed housing to the community spaces. Students strived to reflect the current culture of the community in their designs while adding to the existing beauty of Sisters, Oregon. This report summarizes the three different housing approaches while highlighting details within some of the designs for the affordable housing, adaptive reuse and site design in Sisters, Oregon

The STEM Crisis and Teacher Practice: Exploring Responses to the Competing Discursive Arrangements of Education in the Sciences in a Catholic School Setting

STEM has become a pervasive part of global education reform. The STEM discourse positions the purpose of scientific education as being to prepare young people for work in a hyper-competitive 21st century knowledge economy, pushing aside alternative approaches focussed on interrogating social, moral and political issues in context. This narrative does not always sit comfortably with the holistic ambitions of many state and faith-based education systems. In this paper we will argue that these tensions emerge from deeper conflicts in the cultural-discursive arrangements around education in the advanced democratic states through an exploration of the response to a STEM curriculum project in a Catholic education system. The exploration is based on a phenomenographic analysis of reflective interviews conducted with participating teachers. We conclude that while the teachers are aware of the tensions, they may benefit from access to a language for discussing the various pressures on learning design and meaning making

Revision of the Australian guidelines to reduce health risks from drinking alcohol

Introduction: The Australian guidelines to reduce health risks from drinking alcohol were released in 2020 by the National Health and Medical Research Council. Based on the latest evidence, the guidelines provide advice on how to keep the risk of harm from alcohol low. They refer to an Australian standard drink (10 g ethanol). Recommendations:: •Guideline 1: To reduce the risk of harm from alcohol-related disease or injury, healthy men and women should drink no more than ten standard drinks a week and no more than four standard drinks on any one day. The less you drink, the lower your risk of harm from alcohol. •Guideline 2: To reduce the risk of injury and other harms to health, children and people under 18 years of age should not drink alcohol. •Guideline 3: To prevent harm from alcohol to their unborn child, women who are pregnant or planning a pregnancy should not drink alcohol. For women who are breastfeeding, not drinking alcohol is safest for their baby. Changes as result of the guideline: The recommended limit for healthy adults changed from two standard drinks per day (effectively 14 per week) to ten per week. The new guideline states that the less you drink, the lower your risk of harm from alcohol. The recommended maximum on any one day remains four drinks (clarified from previously “per drinking occasion”). Guidance is clearer for pregnancy and breastfeeding, and for people aged less than 18 years, recommending not drinking.</p

A single inactivating amino acid change in the SARS-CoV-2 NSP3 Mac1 domain attenuates viral replication in vivo

Despite unprecedented efforts, our therapeutic arsenal against SARS-CoV-2 remains limited. The conserved macrodomain 1 (Mac1) in NSP3 is an enzyme exhibiting ADP-ribosylhydrolase activity and a possible drug target. To determine the role of Mac1 catalytic activity in viral replication, we generated recombinant viruses and replicons encoding a catalytically inactive NSP3 Mac1 domain by mutating a critical asparagine in the active site. While substitution to alanine (N40A) reduced catalytic activity by ~10-fold, mutations to aspartic acid (N40D) reduced activity by ~100-fold relative to wild-type. Importantly, the N40A mutation rendered Mac1 unstable in vitro and lowered expression levels in bacterial and mammalian cells. When incorporated into SARS-CoV-2 molecular clones, the N40D mutant only modestly affected viral fitness in immortalized cell lines, but reduced viral replication in human airway organoids by 10-fold. In mice, the N40D mutant replicated at &gt;1000-fold lower levels compared to the wild-type virus while inducing a robust interferon response; all animals infected with the mutant virus survived infection. Our data validate the critical role of SARS-CoV-2 NSP3 Mac1 catalytic activity in viral replication and as a promising therapeutic target to develop antivirals