1,137 research outputs found

    Talent identification and recruitment in youth soccer: Recruiter’s perceptions of the key attributes for player recruitment

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    Using the modified Delphi method, we aimed to understand the attributes youth coaches and recruiters perceive as important when identifying skilled youth performance at the entry level of representative soccer in Australia (i.e., Under 13 years). Furthermore, we also aimed to describe the current methods youth coaches and recruiters use to assess and identify these attributes in youth players. Australian regional youth technical directors and coaches (n = 20) completed a three stage process, including an initial interview and two subsequent questionnaires, whereby attributes and qualities associated with talent identification were rated and justified according to the importance for youth player performance and talent identification. Results indicate a hierarchy of attributes recruiters perceive as important for Under 13 soccer performance, including technical (i.e., first touch, striking the ball, one-versus-one ability, and technical ability under pressure), tactical (i.e., decision-making ability) and psychological attributes (i.e., coachability and positive attitude). In addition, the findings indicated attributes and qualities not emphasised within the talent identification process including, physiological, anthropometrical, sociological and several psychological attributes. It is suggested talent recruiters apply a holistic multidisciplinary approach to talent identification, with the current findings potentially providing initial evidence to suggest recruiters do consider numerous attributes when selecting and identifying youth players

    Controlled functional expression of the bacteriocins pediocin PA-1 and bactofencin A in Escherichia coli

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    peer-reviewedThe bacteriocins bactofencin A (class IId) and pediocin PA-1 (class IIa) are encoded by operons with a similarly clustered gene organization including a structural peptide, an immunity protein, an ABC transporter and accessory bacteriocin transporter protein. Cloning of these operons in E. coli TunerTM (DE3) on a pETcoco-2 derived vector resulted in successful secretion of both bacteriocins. A corresponding approach, involving the construction of vectors containing different combinations of these genes, revealed that the structural and the transporter genes alone are sufficient to permit heterologous production and secretion in this host. Even though the accessory protein, usually associated with optimal disulfide bond formation, was not required for bacteriocin synthesis, its presence did result in greater pediocin PA-1 production. The simplicity of the system and the fact that the associated bacteriocins could be recovered from the extracellular medium provides an opportunity to facilitate protein engineering and the overproduction of biologically-active bacteriocins at industrial scale. Additionally, this system could enable the characterization of new bacteriocin operons where genetic tools are not available for the native producers

    The development of a prototype behavioral marker system for US Navy officers of the deck

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    The officer of the deck (OOD) of a US Navy ship is in charge of the safe and proper operation of the ship, and accountable to the commanding officer for every event that occurs during his or her OOD watch. This paper discusses the development of a prototype behavioral marker system to evaluate, and provide feedback on, the nontechnical (cognitive, social, and personal resource) skills of OODs. An initial set of 17 categories of nontechnical skills were identified from a literature review. A focus group with four qualified OODs used the skills identified from the literature review to develop an initial taxonomy of five categories, each with two or three corresponding behavioral elements. This taxonomy was then used to classify 149 statements concerned with the nontechnical skills of OODs collected from 16 critical incident interviews. After three iterations of adaptations to the taxonomy, two independent raters were able to reach acceptable levels of reliability in using the taxonomy to classify the statements. Although further development work is required, it is suggested that the prototype behavioral marker system has implications for improving safety and performance on military and civilian ships

    Inventory and monitoring of seabirds in National Park of American Samoa

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    Reports were scanned in black and white at a resolution of 600 dots per inch and were converted to text using Adobe Paper Capture Plug-in.This first island-wide inventory and preliminary monitoring of American Samoa seabird populations has produced several significant results. The beginning of a seabird colony catalog has been established in a digital format. The protocol for surveying seabirds around Tutuila Island by boat is described, and future efforts can be compared to baseline results reported here. A detailed survey of the National Park lands around Tutuila is mapped and photographed and seabird distributions found in the 2000 round-island survey and 2003 partial-island survey are discussed. Our results support anecdotal evidence that the inaccessible north shore of Tutuila supports the majority of that island’s resident seabirds. Greater than 90% of Red-footed Boobies and Great Frigatebird observations during the Tutuila round-island survey were made in NPSA areas. The north shore areas are important for coastal cliff nesters such as Brown, Black Noddies and Blue Noddies, a species of international significance. Bridled Terns are extending their pan tropical range to include Tutuila, including Park areas. White Terns and White-tailed Tropicbirds are more evenly distributed across forests in Tutuila within as well as outside NPSA areas.National Park Service Contract No. 8036 2 900

    Does profiling employees online overstep the boundaries?

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    The challenge is to balance employer needs with ensuring safe and private online spaces for staff, argue Paula McDonald, Peter O’Connor and Paul Thompso

    Employee dissent on social media and organizational discipline

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    What kind of surveillance of employees is evident today? The rights of employers to police and act punitively with regard to workplace dissent and misbehaviour have become contentious legal, policy and ethical issues. Drawing on survey responses from employees in the UK and Australia, this study investigates the scope and scale of employee dissent in relation to critical online comments and the private use of social media during work time. The findings reveal a sufficient pool of misbehaviours, albeit that they are emergent and uneven. Also evident were some apparently contradictory responses with respect to employer rights to profile and discipline, at the same time as asserting employee rights to voice and private online identities. The findings contribute to knowledge of how much and what kinds of online dissent exist in the ambiguous space between the public sphere of work and the private lives of individual employees and what employers do about it

    MEME-LaB : motif analysis in clusters

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    Genome-wide expression analysis can result in large numbers of clusters of co-expressed genes. While there are tools for ab initio discovery of transcription factor binding sites, most do not provide a quick and easy way to study large numbers of clusters. To address this, we introduce a web-tool called MEME-LaB. The tool wraps MEME (an ab initio motif finder), providing an interface for users to input multiple gene clusters, retrieve promoter sequences, run motif finding, and then easily browse and condense the results, facilitating better interpretation of the results from large-scale datasets

    Storage of legacy print collections: the views of Australasian university librarians

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    This paper reports on the results of a qualitative survey conducted with seven managers of university libraries from Australia and New Zealand. The purpose of the survey was to explore both library responses to, and librarians’ attitudes towards, issues related to the long-term storage and management of legacy print collections. There is a focus on issues related to future planning for print storage, including the prospects for collaborative storage; the balance between on-site and off-site storage; the impact of mass-digitisation programs; and the desirability of collaboration outside the university library sector

    Recombinant Incretin-Secreting Microbe Improves Metabolic Dysfunction in High-Fat Diet Fed Rodents

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    peer-reviewedThe gut hormone glucagon-like peptide (GLP)-1 and its analogues represent a new generation of anti-diabetic drugs, which have also demonstrated propensity to modulate host lipid metabolism. Despite this, drugs of this nature are currently limited to intramuscular administration routes due to intestinal degradation. The aim of this study was to design a recombinant microbial delivery vector for a GLP-1 analogue and assess the efficacy of the therapeutic in improving host glucose, lipid and cholesterol metabolism in diet induced obese rodents. Diet-induced obese animals received either Lactobacillus paracasei NFBC 338 transformed to express a long-acting analogue of GLP-1 or the isogenic control microbe which solely harbored the pNZ44 plasmid. Short-term GLP-1 microbe intervention in rats reduced serum low-density lipoprotein cholesterol, triglycerides and triglyceride-rich lipoprotein cholesterol substantially. Conversely, extended GLP-1 microbe intervention improved glucose-dependent insulin secretion, glucose metabolism and cholesterol metabolism, compared to the high-fat control group. Interestingly, the microbe significantly attenuated the adiposity associated with the model and altered the serum lipidome, independently of GLP-1 secretion. These data indicate that recombinant incretin-secreting microbes may offer a novel and safe means of managing cholesterol metabolism and diet induced dyslipidaemia, as well as insulin sensitivity in metabolic dysfunction

    The safety climate in primary care (SAP-C) study: study protocol for a randomised controlled feasibility study

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    BACKGROUND: Research on patient safety has focused largely on secondary care settings, and there is a dearth of knowledge relating to safety culture or climate, and safety climate improvement strategies, in the context of primary care. This is problematic given the high rates of usage of primary care services and the myriad of opportunities for clinical errors daily. The current research programme aimed to assess the effectiveness of an intervention derived from the Scottish Patient Safety Programme in Primary Care. The intervention consists of safety climate measurement and feedback and patient chart audit using the trigger review method. The purpose of this paper is to describe the background to this research and to present the methodology of this feasibility study in preparation for a future definitive RCT. METHODS: The SAP-C study is a feasibility study employing a randomised controlled pretest-posttest design that will be conducted in 10 general practices in the Republic of Ireland and Northern Ireland. Five practices will receive the safety climate intervention over a 9-month period. The five practices in the control group will continue care as usual but will complete the GP-SafeQuest safety climate questionnaire at baseline (month 1) and at the terminus of the intervention (month 9). The outcomes of the study include process evaluation metrics (i.e. rates of participant recruitment and retention, rates of completion of safety climate measures, qualitative data regarding participants’ perceptions of the intervention’s potential efficacy, acceptability, and sustainability), patient safety culture in intervention and control group practices at posttest, and instances of undetected patient harm identified through patient chart audit using the trigger review method. DISCUSSION: The planned study investigates an intervention to improve safety climate in Irish primary care settings. The resulting data may inform our knowledge of the frequency of undetected patient safety incidents in primary care, may contribute to improved patient safety practices in primary care settings, and may inform future research on patient safety improvement initiatives. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40814-016-0096-5) contains supplementary material, which is available to authorized users
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