Level of Service and the Transit Neighbourhood - observations from Dublin City and Suburbs

Few studies have looked at the impact Level-of-Service (LOS) might have on the distances people walk to public transport. The relationship, if any, has implications for transit-oriented-development and the viability of different transit modes serving suburban areas. This paper examines pedestrian catchment areas and LOS at across a light rail, a metro rail and two bus corridors in Dublin. Over 700 public transport users were surveyed at 17 stops and their trip origin identified. Catchment areas for bus services with high levels of service were found to be comparable and often greater than those for LRT or metro rail. 65% of all bus trip-origins are more than 500m from stops. A standard distance analysis suggests natural catchment limits of over 1400m for high quality bus, significantly greater than light rail and metro rail of similar service levels. The shape of transit catchment areas are also distinctive and appear to be most influenced by: network density, stopping patterns, urban gravity and quality of service. While further analysis is recommended, public transport users in the Greater Dublin Area appear more influenced by level-of-service than by modal type when deciding how far they are prepared to walk to public transport.Institute of Transport and Logistics Studies. Faculty of Economics and Business. The University of Sydne

Measuring the Economic and Cultural Values of Historic Heritage Places

This research project on the valuation of cultural heritage is developing a methodology for a quantitative valuation study of the use and non-use values of historic heritage places. The methodology developed in this project will provide a template for subsequent empirical applications to generate data of use in the formulation of heritage policies and programs. The initial stages in reaching this larger objective have involved determining how historic heritage places are to be categorised for measurement, establishing the process by which heritage attributes will be determined for valuation, and developing and trialling an effective measurement framework. The principal techniques being developed for valuation assessment in the project are derived from choice-modelling (CM) and contingent-valuation methods (CVM). A particular focus of the project is on the systematic integration of cultural and economic value assessments within a consistent theoretical framework, reflecting the need for an economic perspective on the significance criteria currently in use in heritage policy in Australia.Community/Rural/Urban Development, Environmental Economics and Policy,

On the "renormalization" transformations induced by cycles of expansion and contraction in causal set cosmology

We study the ``renormalization group action'' induced by cycles of cosmic expansion and contraction, within the context of a family of stochastic dynamical laws for causal sets derived earlier. We find a line of fixed points corresponding to the dynamics of transitive percolation, and we prove that there exist no other fixed points and no cycles of length two or more. We also identify an extensive ``basin of attraction'' of the fixed points but find that it does not exhaust the full parameter space. Nevertheless, we conjecture that every trajectory is drawn toward the fixed point set in a suitably weakened sense.Comment: 22 pages, 1 firgure, submitted to Phys. Rev.

Pilot scale implementation of a single-use, high intensity, integrated process system

Pfizer and Boehringer Ingelheim are developing pilot scale systems with entirely single-use flow paths that are fully integrated end-to-end under a single control system from bioreactor through downstream processing. These prototype systems are designed to run continuous processing from the bioreactor to downstream, and periodic processing to the end of the downstream system. This presentation shows the evolution of the systems including some novel single use technologies, details of some high-intensity run results, and offers future single use improvement ideas

Printable devices for neurotechnology

Printable electronics for neurotechnology is a rapidly emerging field that leverages various printing techniques to fabricate electronic devices, offering advantages in rapid prototyping, scalability, and cost-effectiveness. These devices have promising applications in neurobiology, enabling the recording of neuronal signals and controlled drug delivery. This review provides an overview of printing techniques, materials used in neural device fabrication, and their applications. The printing techniques discussed include inkjet, screen printing, flexographic printing, 3D printing, and more. Each method has its unique advantages and challenges, ranging from precise printing and high resolution to material compatibility and scalability. Selecting the right materials for printable devices is crucial, considering factors like biocompatibility, flexibility, electrical properties, and durability. Conductive materials such as metallic nanoparticles and conducting polymers are commonly used in neurotechnology. Dielectric materials, like polyimide and polycaprolactone, play a vital role in device fabrication. Applications of printable devices in neurotechnology encompass various neuroprobes, electrocorticography arrays, and microelectrode arrays. These devices offer flexibility, biocompatibility, and scalability, making them cost-effective and suitable for preclinical research. However, several challenges need to be addressed, including biocompatibility, precision, electrical performance, long-term stability, and regulatory hurdles. This review highlights the potential of printable electronics in advancing our understanding of the brain and treating neurological disorders while emphasizing the importance of overcoming these challenges

Pairing Neutral Cues with Alcohol Intoxication: New Findings in Executive and Attention Networks

Rationale: Alcohol-associated stimuli capture attention, yet drinkers differ in the precise stimuli that become paired with intoxication. Objectives: Extending our prior work to examine the influence of alcoholism risk factors, we paired abstract visual stimuli with intravenous alcohol delivered covertly and examined brain responses to these Pavlovian conditioned stimuli in fMRI when subjects were not intoxicated. Methods: Sixty healthy drinkers performed task-irrelevant alcohol conditioning that presented geometric shapes as conditioned stimuli. Shapes were paired with a rapidly rising alcohol limb (CS+) using intravenous alcohol infusion targeting a final peak breath alcohol concentration of 0.045 g/dL or saline (CS−) infusion at matched rates. On day two, subjects performed monetary delay discounting outside the scanner to assess delay tolerance and then underwent event-related fMRI while performing the same task with CS+, CS−, and an irrelevant symbol. Results: CS+ elicited stronger activation than CS− in frontoparietal executive/attention and orbitofrontal reward-associated networks. Risk factors including family history, recent drinking, sex, and age of drinking onset did not relate to the [CS+ > CS−] activation. Delay-tolerant choice and [CS+ > CS−] activation in right inferior parietal cortex were positively related. Conclusions: Networks governing executive attention and reward showed enhanced responses to stimuli experimentally paired with intoxication, with the right parietal cortex implicated in both alcohol cue pairing and intertemporal choice. While different from our previous study results in 14 men, we believe this paradigm in a large sample of male and female drinkers offers novel insights into Pavlovian processes less affected by idiosyncratic drug associations

Male Sexual Preference for Female Swimming Activity in the Guppy (Poecilia reticulata)

Mate choice that is based on behavioural traits is a common feature in the animal kingdom. Using the Trinidadian guppy, a species with mutual mate choice, we investigated whether males use female swimming activity—a behavioural trait known to differ consistently among individuals in many species—as a trait relevant for their mate choice. In the first experiment, we assessed male and female activity in an open field test alone (two repeated measures) and afterwards in heterosexual pairs (two repeated measures). In these pairs, we simultaneously assessed males’ mating efforts by counting the number of sexual behaviours (courtship displays and copulations). Male and female guppies showed consistent individual differences in their swimming activity when tested both alone and in a pair, and these differences were maintained across both test situations. When controlling for male swimming behaviour and both male and female body size, males performed more courtship displays towards females with higher swimming activity. In a second experiment, we tested for a directional male preference for swimming activity by presenting males video animations of low- and high-active females in a dichotomous choice test. In congruence with experiment 1, we found males to spend significantly more time in association with the high-active female stimulus. Both experiments thus point towards a directional male preference for higher activity levels in females. We discuss the adaptive significance of this preference as activity patterns might indicate individual female quality, health or reproductive state while, mechanistically, females that are more active might be more detectable to males as well.Leibniz-GemeinschaftDeutsche ForschungsgemeinschaftPeer Reviewe

Identification and characterisation of capidermicin, a novel bacteriocin produced by Staphylococcus capitis

peer-reviewedOne hundred human-derived coagulase negative staphylococci (CoNS) were screened for antimicrobial activity using agar-based deferred antagonism assays with a range of indicator bacteria. Based on the findings of the screen and subsequent well assays with cell free supernatants and whole cell extracts, one strain, designated CIT060, was selected for further investigation. It was identified as Staphylococcus capitis and herein we describe the purification and characterisation of the novel bacteriocin that the strain produces. This bacteriocin which we have named capidermicin was extracted from the cell-free supernatant of S. capitis CIT060 and purified to homogeneity using reversed-phase high performance liquid chromatography (RP-HPLC). Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometric (MS) analysis revealed that the capidermicin peptide has a mass of 5,464 Da. Minimal inhibitory concentration (MIC) experiments showed that capidermicin was active in the micro-molar range against all the Gram-positive bacteria that were tested. Antimicrobial activity was retained over a range of pHs (2–11) and temperatures (10–121°C x 15 mins). The draft genome sequence of S. capitis CIT060 was determined and the genes predicted to be involved in the biosynthesis of capidermicin were identified. These genes included the predicted capidermicin precursor gene, and genes that are predicted to encode a membrane transporter, an immunity protein and a transcriptional regulator. Homology searches suggest that capidermicin is a novel member of the family of class II leaderless bacteriocins