The interplay between tissue growth and scaffold degradation in engineered tissue constructs

In vitro tissue engineering is emerging as a potential tool to meet the high demand for replacement tissue, caused by the increased incidence of tissue degeneration and damage. A key challenge in this field is ensuring that the mechanical properties of the engineered tissue are appropriate for the in vivo environment. Achieving this goal will require detailed understanding of the interplay between cell proliferation, extracellular matrix (ECM) deposition and scaffold degradation.\ud \ud In this paper, we use a mathematical model (based upon a multiphase continuum framework) to investigate the interplay between tissue growth and scaffold degradation during tissue construct evolution in vitro. Our model accommodates a cell population and culture medium, modelled as viscous fluids, together with a porous scaffold and ECM deposited by the cells, represented as rigid porous materials. We focus on tissue growth within a perfusion bioreactor system, and investigate how the predicted tissue composition is altered under the influence of (i) differential interactions between cells and the supporting scaffold and their associated ECM, (ii) scaffold degradation, and (iii) mechanotransduction-regulated cell proliferation and ECM deposition.\ud \ud Numerical simulation of the model equations reveals that scaffold heterogeneity typical of that obtained from μCT scans of tissue engineering scaffolds can lead to significant variation in the flow-induced mechanical stimuli experienced by cells seeded in the scaffold. This leads to strong heterogeneity in the deposition of ECM. Furthermore, preferential adherence of cells to the ECM in favour of the artificial scaffold appears to have no significant influence on the eventual construct composition; adherence of cells to these supporting structures does, however, lead to cell and ECM distributions which mimic and exaggerate the heterogeneity of the underlying scaffold. Such phenomena have important ramifications for the mechanical integrity of engineered tissue constructs and their suitability for implantation in vivo

Optical spectroscopy of faint gigahertz peaked spectrum sources

We present spectroscopic observations of a sample of faint Gigahertz Peaked Spectrum (GPS) radio sources drawn from the Westerbork Northern Sky Survey (WENSS). Redshifts have been determined for 19 (40%) of the objects. The optical spectra of the GPS sources identified with low redshift galaxies show deep stellar absorption features. This confirms previous suggestions that their optical light is not significantly contaminated by AGN-related emission, but is dominated by a population of old (>9 Gyr) and metal-rich (>0.2 [Fe/H]) stars, justifying the use of these (probably) young radio sources as probes of galaxy evolution. The optical spectra of GPS sources identified with quasars are indistinguishable from those of flat spectrum quasars, and clearly different from the spectra of Compact Steep Spectrum (CSS) quasars. The redshift distribution of the GPS quasars in our radio-faint sample is comparable to that of the bright samples presented in the literature, peaking at z ~ 2-3. It is unlikely that a significant population of low redshift GPS quasars is missed due to selection effects in our sample. We therefore claim that there is a genuine difference between the redshift distributions of GPS galaxies and quasars, which, because it is present in both the radio-faint and bright samples, can not be due to a redshift-luminosity degeneracy. It is therefore unlikely that the GPS quasars and galaxies are unified by orientation, unless the quasar opening angle is a strong function of redshift. We suggest that the GPS quasars and galaxies are unrelated populations and just happen to have identical observed radio-spectral properties, and hypothesise that GPS quasars are a sub-class of flat spectrum quasars.Comment: LaTeX, 13 pages. Accepted by MNRAS. For related papers see http://www.ast.cam.ac.uk/~snelle

Quasars, their host galaxies, and their central black holes

We present the final results from our deep HST imaging study of the hosts of radio-quiet quasars (RQQs), radio-loud quasars (RLQs) and radio galaxies (RGs). We describe new WFPC2 R-band observations for 14 objects and model these images in conjunction with the data already reported in McLure et al (1999). We find that spheroidal hosts become more prevalent with increasing nuclear luminosity such that, for nuclear luminosities M_V < -23.5, the hosts of both radio-loud and radio-quiet AGN are virtually all massive ellipticals. Moreover we demonstrate that the basic properties of these hosts are indistinguishable from those of quiescent, evolved, low-redshift ellipticals of comparable mass. This result kills any lingering notion that radio-loudness is determined by host-galaxy morphology, and also sets severe constraints on evolutionary schemes which attempt to link low-z ULIRGs with RQQs. Instead, we show that our results are as expected given the relationship between black-hole and spheroid mass established for nearby galaxies, and apply this relation to estimate the mass of the black hole in each object. The results agree very well with completely-independent estimates based on nuclear emission-line widths; all the quasars in our sample have M(bh) > 5 x 10^8 solar masses, while the radio-loud objects are confined to M(bh) > 10^9 solar masses. This apparent mass-threshold difference, which provides a natural explanation for why RQQs outnumber RLQs by a factor of 10, appears to reflect the existence of a minimum and maximum level of black-hole radio output which is a strong function of black-hole mass. Finally, we use our results to estimate the fraction of massive spheroids/black-holes which produce quasar-level activity. This fraction is \~0.1% at the present day, rising to > 10% at z = 2-3.Comment: Revised version accepted for publication in Monthly Notices of the Royal Astronomical Society. 46 pages, the final 19 of which comprise an Appendix. 15 figures in main text. A further 14 4-panel greyscale plots and 14 line plots which appear in the Appendix have been reproduced here with reduced quality due to space limitations. A full resolution copy of the manuscript can be obtained via ftp://ftp.roe.ac.uk/pub/jsd/dunlop2002.ps.g

Observations of HI Absorbing Gas in Compact Radio Sources at Cosmological Redshifts

We present an overview of the occurrence and properties of atomic gas associated with compact radio sources at redshifts up to z=0.85. Searches for HI 21cm absorption were made with the Westerbork Synthesis Radio Telescope at UHF-high frequencies (725-1200 MHz). Detections were obtained for 19 of the 57 sources with usable spectra (33%). We have found a large range in line depths, from tau=0.16 to tau<=0.001. There is a substantial variety of line profiles, including Gaussians of less than 10km/s, to more typically 150km/s, as well as irregular and multi-peaked absorption profiles, sometimes spanning several hundred km/s. Assuming uniform coverage of the entire radio source, we obtain column depths of atomic gas between 1e19 and 3.3e21(Tsp/100K)(1/f)cm^(-2). There is evidence for significant gas motions, but in contrast to earlier results at low redshift, there are many sources in which the HI velocity is substantially negative (up to v=-1420km/s) with respect to the optical redshift, suggesting that in these sources the atomic gas, rather than falling into the centre, may be be flowing out, interacting with the jets, or rotating around the nucleus.Comment: 10 pages, accepted for publication in A&

HST/STIS low dispersion spectroscopy of three Compact Steep Spectrum sources Evidence for jet-cloud interaction

We present Hubble Space Telescope Imaging Spectrograph long-slit spectroscopy of the emission line nebulae in the compact steep spectrum radio sources 3C 67, 3C 277.1, and 3C 303.1. We derive BPT (Baldwin- Philips-Terlevich; Baldwin et al. 1981) diagnostic emission line ratios for the nebulae which are consistent with a mix of shock excitation and photoionization in the extended gas. In addition, line ratios indicative of lower ionization gas are found to be associated with higher gas velocities. The results are consistent with a picture in which these galaxy scale radio sources interact with dense clouds in the interstellar medium of the host galaxies, shocking the clouds thereby ionizing and accelerating them.Comment: Accepted for publication in A&A. 6 pages, 8 figure

Evidence for Ordered Magnetic Fields in the Quasar Environment

At a distance of 20 pc from the purported supermassive black hole powering quasars, temperatures and densities are inferred from optical observations to be ~10**4 K and ~10**4 cm**-3. Here we present Very Long Baseline Interferometry radio observations revealing organized magnetic fields on the parsec scale in the hot plasma surrounding the quasar OQ172 (1442+101). These magnetic fields rotate the plane of polarization of the radio emission coming from the core and inner jet of the quasar. The derived rotation measure (RM) is 40,000 rad m**-2 in the rest frame of the quasar. Only 10 mas (a projected distance of 68 pc) from the nucleus the jet absolute values of RM fall to less than 100 rad m**-2.Comment: in press at ApJ Letters, 12 page LaTeX document includes 4 postscript figure

A century of limnological evolution and interactive threats in the Panama Canal: Long-term assessments from a shallow basin

Large tropical river dam projects are expected to accelerate over the forthcoming decades to satisfy growing demand for energy, irrigation and flood control. When tropical rivers are dammed the immediate impacts are relatively well studied, but the long-term (decades-centuries) consequences of impoundment remain poorly known. We combined historical records of water quality, river flow and climate with a multi-proxy (macrofossils, diatoms, biomarkers and trace elements) palaeoecological approach to reconstruct the limnological evolution of a shallow basin in Gatun Lake (Panama Canal, Panama) and assess the effects of multiple linked factors (river damming, forest flooding, deforestation, invasive species, pollution and hydro-climate) on the study area. Results show that a century after dam construction, species invasion, deforestation and salt intrusions have forced a gradual change in the study basin from a swamp-type environment towards a more saline lake-governed system of benthic–littoral production likely associated with the expansion of macrophyte stands. Hydrology still remains the most important long-term (decades) structural factor stimulating salinity intrusions, primary productivity, deposition of minerals, and reduction of water transparency during wet periods. During dry periods, physical-chemical conditions are in turn linked to clear water and aerobic conditions while nutrients shift to available forms for the aquatic biota in the detrital-rich reductive sediments. Our study suggests that to preserve the natural riverine system functioning of this area of the Panama Canal, management activities must address long-term ecosystem structural drivers such as river flow, runoff patterns and physical-chemical conditions

Very Large Baseline Array observations of Mrk 6 : probing the jet-lobe connection

We present the results of high-resolution VLBI (very long baseline interferometry) observations at 1.6 and 4.9 GHz of the radio-loud Seyfert galaxy, Mrk 6. These observations are able to detect a compact radio core in this galaxy for the first time. The core has an inverted spectral index (α1.6 4.9 = +1.0 ± 0.2) and a brightness temperature of 1 × 108 K. Three distinct radio components, which resemble jet elements and/or hotspots, are also detected. The position angles of these elongated jet elements point not only to a curved jet in Mrk 6, but also towards a connection between the AGN and the kpc-scale radio lobes/bubbles in this galaxy. Firmer constraints on the star formation rate provided by new Herschel observations (SFR <0.8 M⊙ yr-1) make the starburst-wind-powered bubble scenario implausible. From plasma speeds, obtained via prior Chandra X-ray observations, and ram pressure balance arguments for the interstellar medium and radio bubbles, the north-south bubbles are expected to take 7.5 × 106 yr to form, and the east-west bubbles 1.4 × 106 yr. We suggest that the jet axis has changed at least once in Mrk 6 within the last ≈107 yr. A comparison of the nuclear radio-loudness of Mrk 6 and a small sample of Seyfert galaxies with a subset of low-luminosity FR I radio galaxies reveals a continuum in radio properties.Peer reviewe

The follow-up EVN observations of twelve GPS radio sources at 5 GHz

We defined a sub-sample of twelve GPS sources which have not been observed with the VLBI before, from the Parkes half-Jansky sample, and carried out VLBI observations at 1.6 GHz and 5 GHz with the European VLBI Network (EVN) in 2006 and 2008, respectively, to classify the source structure and to find compact symmetric objects (CSOs). Additionally, we carried out the 4.85 GHz flux density observations for these sources with the Urumqi 25-m telescope between the years 2007 and 2009 to study whether there is any variability in the total flux density of the GPS sources. The results of the 5 GHz VLBI observations and total flux densities of these sources are presented in this paper. From the VLBI morphologies, the spectral indices of components and the total flux variability of the twelve targets, we firmly classify three sources J0210+0419, J1135$-$0021, and J2058+0540 as CSOs, and classify J1057+0012, J1203+0414, and J1600$-$0037 as core-jet sources. The others J0323+0534, J0433$-$0229, J0913+1454, J1109+1043, and J1352+0232 are labelled CSO candidates, and J1352+1107 is a complex feature. Apart from core-jet sources, the total flux densities of the CSOs and candidates are quite stable at 5 GHz both during a long-term of $\sim$20 years relative to the PKS90 data and in a period between 2007 and 2009. The total flux densities are resolved-out by more than 20\% in the 5 GHz VLBI images for 6 sources, probably because of diffuse emission. In addition, we estimated the jet viewing angles $\Theta$ for the confirmed CSOs by using the double-lobe flux ratio of the sources, the result being indicative of relatively large $\Theta$ for the CSOs.Comment: 9 pages, 12 figures, accepted for publication in A&A

Community views on ‘Can perinatal services safely identify Aboriginal and Torres Strait Islander parents experiencing complex trauma?’

Family and extended kinship systems which nurture healthy, happy children are central to Aboriginal and Torres Strait Islander cultures. Since colonisation, Aboriginal and Torres Strait Islander communities have been impacted by intergenerational cycles of trauma, stemming from colonial violence, genocidal policies and discrimination, including the forced removal of children from their families. Becoming a parent offers a unique life-course opportunity for trauma recovery and preventing intergenerational trauma. However, identifying or ‘recognising’ complex trauma carries significant risk of harm for Aboriginal and Torres Strait Islander parents due to reactive prenatal child protection involvement potentially compounding experiences of trauma, and limited benefits due to lack of culturally appropriate support. The Aboriginal-led participatory Healing the Past by Nurturing the Future project aims to co-design safe, accessible and feasible perinatal awareness, recognition, assessment and support strategies for Aboriginal and Torres Strait Islander parents experiencing complex trauma. This paper presents views of 38 workshop participants to determine prerequisites for ensuring benefits outweigh risks of assessment to safely recognise parents experiencing complex trauma, consistent with screening criteria. Six essential elements were identified from thematic analysis: high-quality holistic care; cultural, social and emotional safety; empowerment, choice and control; flexible person-centred approaches; trusting relationships; and sensitive, skilled communication