ISOLASI DAN UJI PENANGKAPAN RADIKAL BEBAS DPPH OLEH ISOLAT-1, FRAKSI ETIL ASETAT, DAN EKSTRAK ETANOL AKAR PASAK BUMI (Eurycoma longifolia Jack)

The free radical was produced when undertaking processes metabolit or opposedinfection and when the body dissolved food. The unstable free radical could beneutralised with antioxidant. The root of the pasak bumi (Eurycoma longifolia Jack)was known contained the compound flavonoid, that it was known had the activity asantioxidant. This research aimed at knowing the activity capacity of the capture of thefree radical the water fraction and the faction ethyl acetate fraction the extract ethanolof the root of the pasak bumi by using the DPPH method.The root of the pasak bumi was extracted with ethanol 70% used maceration. Theethanolic extract was dissolved in ethyl acetate, fractination with ethyl acetate so as tobe received by the fraction ethyl acetate. The concentration of the ethanolic extract andthe fraction of ethyl acetate that was used were 2; 4; 8; 16 ug/mL and isolat-1 that is:0.8; 1.6; 3.2; and 6.4 ug/mL.Results of the research showed that all the treatments had the activity asscavenging the free radical. Results of the analysis statistika with the Kruskal Wallismethod in the level of the confident 95% that was followed by the test Mann Whitneyshowed the existence of the difference of the activity of the capture of the free radicalwho was significant between respectively the treatment group. The conclusion from thisresearch was the value ES50 ethanalic extract (15.64 ug/mL) bigger than the factionethyl acetate fraction and higher (13.948 ug/mL) value of ethanalic extract was thanisolat 1 (3.961 g/mL)

UJI SITOTOKSISITAS DAN ANTIPROLIFERATIF FRAKSI ETIL ASETAT EKSTRAK ETANOL BIJI JINTEN HITAM (Nigella sativa, Lour) TERHADAP SEL MIELOMA

Cancer is the formation of new tissue which is abnormal and malignant. A group of cells suddenly become disorganized and reduplicate themselves rigorously (hyperproliferation). Nigella sativa L. is one of the herbs which have an anticancer effect. This research aims to assess the cytotoxic and antiproliferative effect of Nigella sativa L. ethanol extract of Myeloma cells. Ethanolic extract was produced from Nigella sativa L. powder with maseration method. The cytotoxicity test was done by incubating Myeloma cells with the treatment concentration group of N. sativa L. ethyl acetic fraction of ethanolic extract 2000; 1000; 500; 250; and 62.5 µg/ml, respectively. The test was done with an MTT method and then with a calculation of its death percentage. The LC50 is calculated using a probit analysis method. The test was then continued with the antiproliferative test to assess the doubling time at treatment concentration 125; 62.5 µg/ml and cellular control at hours 24, 48, and 72. The results showed that Nigella sativa L. ethanolic extract had cytotoxic activity towards the Mieloma cells with an LC50 value 177.01 µg/ml. The antiproliferative test showed that there was a growth inhibition, even cell death at the extract treatments. The doubling time was 253 hours at 62.5 µg/ml concentration, 298.4 hours at 125 ug/ml, while the cell control had 54.52 hour

UJI SITOTOKSITAS DAN ANTIPROLIFERATIF SEL KANKER PAYUDARA T47D DAN SEL VERO BIJI Nigella sativa, L.

Black cumin seeds contain oil classes atisiri, terpenes, and alkaloids that can beused for traditional medicine as anticancer. The purpose of this study was to assess thecytotoxic effects of ether extract, ethanol, and infusa seed Nigella sativa, L. (Blackcumin) to inhibit the growth of T47D and normal (Vero) cells and its effect on thekinetics of T47D breast cancer cells proliferation. This study were used extracts ofether, ethanol, and black cumin seeds infusa that obtained by maceration method andinfundasi. Cytotoxicity test was performed by incubating T47D breast cancer cells at a2 x 104 density with concentrations series of 2000; 1000; 500: 250: 125; 62.5; 31.25and 15.625 microg/ml for 24 hours. Vero cell with a density of 2 x 104 withconcentrations series of 4000; 2000; 1000; 500; 250; 125 and 62.5 microg/ml. Thenumber of cells was calculated by direct counting method and the calculated the deathpercentage. The LC50 values calculated using probit analysis. Observations on thenature of the growth inhibition were done by observing kinetics of cell proliferationwith the addition of trypan blue at-24, 48 and 72 to determine its doubling time. Theresults showed that the ether extract, ethanol, and black cumin seeds infusa arecytotoxic to T47D breast cancer cells with successive LC50 of 32.63: 10.02, and 23.82mg mL. Vero cell cytotoxicity test to produce successive LC50 of 300.6; 328.41, and778.64 g/ml. Antiproliferative test results showed that in 62.5 ug/ml and 31.625microg/ml prolong the doubling time. Ethanol extract of cumin seeds have a higherpotential due to the highest security index compared to ether extract and infusa

The inhibition of CYP 1A1 Expression in Mice By Melatonin

Lung cancer is a leading cause of death among the other types of cancer in the world in 2010. The impact of genetic polymorphisms in CYP1A1 on the susceptibility of lung cancer has become particular interest in recent years, since this sub-enzyme plays a major role in activation of major classes of tobacco carcinogens. Melatonin has been proved to modulate the effects of cancer chemotherapy, by enhancing its therapeutic efficacy and reducing its toxicity. This study aimed to assess CYP 1A1 expression which is modulated by melatonin. Mice are divided into negative control group (without melatonin), melatonin 25 mg /kg and 125 mg melatonin / kg BW. Cancer induced by using Cadmium. The parameters determined are the results of electrophoresis the band color intensity of bands of CYP 1A1 gene which is resulted from PCR (Polymerase Chain Reaction) between the control group, melatonin dose of 25 mg/kg, and melatonin doses of 50 mg/kg. Electrophoresis results showed that the CYP1A1 band intensity of the melatonin-induced rat weaker than the negative control. This suggests that the rats with melatonin isolates treatment group reduce expression of CYP 1A1 compared to group without melatonin. The conclusions of this study is that melatonin is able to suppress the activation of potentially toxic metabolites thus potential as anticancer. Key words: CYP 1A1, melatonin, cance

CEK SIMILARITY "ISOLASI DAN UJI PENANGKAPAN RADIKAL BEBAS DPPH OLEH ISOLAT-1, FRAKSI ETIL ASETAT,DAN EKSTRAK ETANOL AKAR PASAK BUMI(Eurycoma longifolia Jack)"

Radikal bebas dihasilkan pada waktu menjalankan proses proses metabolit atau melawan infeksi maupun sewaktu tubuh mencerna makanan. Radikal bebas yang tidak stabil dapat dinetralisir dengan antioksidan. Akar pasak bumi (Eurycoma longifolia Jack.) diketahui mengandung senyawa flavonoid, yang diketahui mempunyai aktivitas sebagai antioksidan. Penelitian ini bertujuan untuk mengetahui kemampuan aktivitas penangkapan radikal bebas fraksi air dan fraksi etil asetat ekstrak etanol akar pasak bumi dengan menggunakan metode DPPH (1,1-difenil-2-pikrilhidrazil). Akar pasak bumi diekstraksi dengan etanol 70% menggunakan maserasi. Ekstrak etanol dilarutkan dalam etil asetat, fraksinasi dengan etil asetat sehingga diperoleh fraksi etil asetat. Konsentrasi ekstrak etanol dan fraksi etil asetat yang digunakan adalah 2; 4; 8;16 ug/mL dan isolat-1 yaitu: 0,8; 1,6; 3,2; dan 6,4 ug/mL. Hasil penelitian menunjukkan bahwa semua perlakuan mempunyai aktivitas sebagai penangkap radikal bebas. Hasil analisis statistika dengan metode Kruskal Wallis pada taraf kepercayaan 95% yang dilanjutkan dengan uji Mann whitney menunjukkan adanya perbedaan aktivitas penangkapan radikal bebas yang signifikan antara masing-masing kelompok perlakuan. Kesimpulan dari penelitian ini adalah diperolehnya harga ES50 ektrak etanol (15,64 ug/mL) lebih besar daripada fraksi etil asetat (13,948 ug/mL) lebih besar daripada isolat 1 (3,961). Kata kunci: Eurycoma longifolia Jack., radikal bebas, DPPH

CEK SIMILARITY "ANTIOXIDANT ACTIVITY ASSAY OF ETHANOLIC EXTRACT OF SIRSAK (Annona muricta L) LEAVES"

Background. Free radicals cause cell`s damage in the body which manifestate as disease. The increase prevalence of degeneratife diseases caused by free radicals in Indonesia has motivated scientists to explore natural antioxidant compounds. Sirsak (Annona muricata L) is one of plant used as anticonvulsant, antioxidant, and anticancer. Objective. This study was purposed to investigate antioxidant activity of ethanolic extract of Annona muricata L Methods. This research comprised ethanolic extract of Sirsak leaves using maceration method and antioxidant in vitro examintation used 2,5; 5; 10; 20; and 40 ug/mL of DPPH. The method used for antioxidant assessment was true ability of antioxidant to capture DPPH. Outcome measured. ES50 of DPPH technique Results. The ES50 result of ethanolic extract on Sirsak leaves was 22.23 ± 0,64 µg/mL Key words : Annona muricata, DPPH, antioxidan

UJI SITOTOKSISITAS, ANTIPROLIFERATIF, DAN PENGARUHNYA TERHADAP EKSPRESI P53 DAN BCl2 DARI FRAKSI ETANOL INFUSA DAUN TEH (Camellia sinensis (L.) O.K.) TERHADAP SEL HeLa

Tea (Camellia sinensis  (L.) O.K.)  is one of medical plant traditionally used by society as anticancer. The aim of this research is to evaluate the citotoxic and antiproliferative effect of ethanolic fraction of tea (Camellia sinensis  (L.) O.K.) and its effect on P53 and bcl-2. Tea were collected and extracted by infundation and fractioned with ethanol as solvent. Citotoxicity test was done by incubating HeLa cell at a density of 2.104 with treatment using extract in concentration of 250; 125 ; 62,5; 31,25; 15,63; and 7,81 µg/mL during 24 hours.  Antiproliferative test were done by calculating doubling time which was equaling a life cell on treatment sample concentration 31,25 µg/mL; 15,63 µg/mL; 7,81 µg/mL; and 3,91 µg/mL with cell control at 24, 48 and 72 hours.  Imunohistochemistry assay was used in LC50  with p53 and bcl-2 antibody. P53 and bcl-2 expression is compared to control. The result indicated that ethanol fraction of tea (Camellia sinensis  (L.) O.K.) leaf infuse has citotoxicity effect on HeLa cell with LC50 of 24,45 µg/mL. The result of  doubling time test indicated that doubling time value for a solvent  control is 74,11 hours and a cell control is 78,22 hours. While, with treatment 31,25 µg/mL; 15,63 µg/mL; 7,81 µg/mL; and 3,91 µg/mL resulted on negative slope then did not produce doubling time. The extract can induce p53 expression and inhibit bcl-2  expression compared to control.Daun teh (Camellia sinensis  (L.) O.K.) merupakan salah satu bahan alam yang digunakan masyarakat untuk pengobatan tradisional sebagai antikanker. Penelitian ini bertujuan untuk mengetahui efek sitotoksisitas, antiproliferatif, dan mekanisme terhadap p53 dan bcl-2  dari fraksi etanol infusa daun teh (Camellia sinensis  (L.) O.K.). Daun teh yang diperoleh, diekstraksi dengan cara infundasi dan difraksinasi dengan pelarut etanol. Uji sitotoksisitas dilakukan dengan menginkubasi sel HeLa dengan kepadatan 2.104 dengan perlakuan ekstrak kadar 250 µg/mL; 125  µg/mL; 62,5; 31,25; 15,63; dan 7,81 µg/mL selama 24 jam. Uji antiproliferatif dilakukan dengan menghitung  jumlah sel hidup pada perlakuan sampel kadar 31,25; 15,63; 7,81; dan 3,91 µg/mL setelah diinkubasi pada jam ke-24, 48 dan 72. Uji imunositokimia dilakukan pada konsentrasi sebesar 24,45 µg/mL  dengan antibodi p53 dan bcl-2.  Ekspresi p53 dan bcl-2   dibandingkan dengan kontrol. Hasil penelitian menunjukkan bahwa sampel fraksi etanol dari infusa daun teh (Camellia sinensis  (L.) O.K.) bersifat sitotoksik terhadap sel HeLa dengan harga LC­­50 sebesar 24, 45 µg/mL. Hasil uji doubling time diperoleh doubling time kontrol pelarut 74,11 µg/mL dan kontrol sel 78,22 µg/mL. Sedangkan pada perlakuan kadar 31,25; 15,63; 7,81; dan 3,91 µg/mL diperoleh harga slope yang negatif sehingga tidak diperoleh harga doubling time.  Ekstrak tersebut dapat memacu ekspresi p53 dan menghambat ekspresi bcl-2 dibandingkan dengan kontrol

CEK SIMILARITY "UJI SITOTOKSISITAS DAN ANTIPROLIFERATIF FRAKSI ETIL ASETAT EKSTRAK ETANOL BIJI JINTEN HITAM (Nigella sativa, Lour) TERHADAP SEL MIELO"

Kanker merupakan penyakit karena sel yang berproliferasi secara pesat dan terus-menerus (proliferasi). Jinten hitam (Nigella sativa, L.) merupakan salah satu tanaman yang berkhasiat sebagai antikanker. Penelitian ini bertujuan untuk mengetahui aktivitas sitotoksisitas dan antiproliferasi ekstrak etanol dari biji jinten hitam (Nigella sativa, L.) terhadap sel Mieloma. Ekstrak etanol diperoleh dari serbuk biji jinten hitam dengan metode penyarian maserasi yang selanjutnya dilakukan fraksinasi dengan etil asetat. Uji sitotoksisitas dilakukan dengan menginkubasi sel Mieloma dengan perlakuan ekstrak etanol biji jinten hitam (Nigella Sativa, L.) dengan beberapa seri kadar yaitu 2000; 1000; 500; 250 dan 62,5 µg/ml. Pengujian dilakukan dengan metode MTT kemudian dihitung persen kematiannya. Nilai LC50 dihitung dengan menggunakan analisis probit. Penelitian dilanjutkan dengan uji antiproliferasi dengan menentukan doubling time pada perlakuan sampel kadar 125 µg/ml dan 62,5 µg/ml dengan kontrol sel pada jam ke-24, 48, dan 72. Hasil penelitian menunjukkan bahwa ekstrak etanol biji jinten hitam bersifat sitotoksik terhadap sel Mieloma dengan harga LC50 sebesar 177,01 µg/ml. Hasil uji antiproliferasi menunjukkan adanya penghambatan pertumbuhan. Nilai doubling time sebesar 253 jam pada kadar 62,5 µg/ml, 298,4 jam pada kadar 125 µg/ml sedangkan pada kontrol sel adalah 54,52 jam. Kata kunci : Kanker, biji Jinten Hitam (Nigella sativa L.), sitotoksi

CEK SIMILARITY "ANTIOXIDANT ACTIVITY ASSAY OF ETHANOLIC EXTRACT OF SIRSAK (Annona muricta L) LEAVES"

Background. Free radicals cause cell`s damage in the body which manifestate as disease. The increase prevalence of degeneratife diseases caused by free radicals in Indonesia has motivated scientists to explore natural antioxidant compounds. Sirsak (Annona muricata L) is one of plant used as anticonvulsant, antioxidant, and anticancer. Objective. This study was purposed to investigate antioxidant activity of ethanolic extract of Annona muricata L Methods. This research comprised ethanolic extract of Sirsak leaves using maceration method and antioxidant in vitro examintation used 2,5; 5; 10; 20; and 40 ug/mL of DPPH. The method used for antioxidant assessment was true ability of antioxidant to capture DPPH. Outcome measured. ES50 of DPPH technique Results. The ES50 result of ethanolic extract on Sirsak leaves was 22.23 ± 0,64 µg/mL Key words : Annona muricata, DPPH, antioxidan