Effect of Piper betle and Brucea javanica on the Differential Expression of Hyphal Wall Protein (HWP1) in Non-Candida albicans Candida (NCAC) Species

The study aimed to identify the HWP1 gene in non-Candida albicans Candida species and the differential expression of HWP1 following treatment with Piper betle and Brucea javanica aqueous extracts. All candidal suspensions were standardized to 1 x 10(6) cells/mL. The suspension was incubated overnight at 37 degrees C (C. parapsilosis, 35 degrees C). Candidal cells were treated with each respective extract at 1, 3, and 6 mg/mL for 24 h. The total RNA was extracted and reverse transcription-polymerase chain reaction was carried out with a specific primer of HWP1. HWP1 mRNAs were only detected in C. albicans, C. parapsilosis, and C. tropicalis. Exposing the cells to the aqueous extracts has affected the expression of HWP1 transcripts. C. albicans, C. parapsilosis, and C. tropicalis have demonstrated different intensity of mRNA. Compared to P. betle, B. javanica demonstrated a higher suppression on the transcript levels of HWP1 in all samples. HWP1 was not detected in C. albicans following the treatment of B. javanica at 1 mg/mL. In contrast, C. parapsilosis and C. tropicalis were shown to have HWP1 regulation. However, the expression levels were reduced upon the addition of higher concentration of B. javanica extract. P. betle and B. javanica have potential to be developed as oral health product.Article Link : http://www.hindawi.com/journals/ecam/2013/397268

Future Community-Based Ecotourism (CBET) development

Ecotourism is an alternative form of tourism and is usually confused with natural and cultural tourism.CBET is fast becoming a popular biodiversity conservation tool that develops and benefits the local community. Based on the context of conservation theory and practice, Community-Based Ecotourism (CBET) is a form of community-based natural resource management. However, a sustainable CBET development through Community Capacity Building (CCB)programs is not something that it easily achievable. Local community’s capacity varies from one culture to another. It takes a high level of community participation, in order for it to come to a level where the community members themselves are motivated to participate and contribute to the development of the program. This fully qualitative research involved 15 respondents from the community of Kg. Selai, Bekok in Johor, Malaysia. The result show there are five factors that sustained the ecotourism development based for Orang Asli Community in Kg. Selai, namely, existing CBET development, past CBET development, local community participation in planning stage of tourism, local participation in implementation stage of tourism and participation in nature conservation

Amalan pengurusan keselamatan bengkel kemahiran hidup (KHB) Sekolah Menengah Harian Daerah Batu Pahat

Kajian ini dijalankan untuk mengkaji sejauhmanakah persepsi pelajar terhadap amalan pengurusan keselamatan dari aspek keselamatan alatan dan bahan, diri dan rakan serta keselamatan am di bengkel Kemahiran Hidup sekolah menengah harian daerah Batu Pahat. Kajian berbentuk kuantitatif dilaksanakan di empat buah sekolah menengah harian daerah Batu Pahat iaitu SMK Datin Onn Jaafar, SMK Banang Jaya, SMK Dato Onn dan SMK Seri Gading. Sampel kajian terdiri daripada 350 orang pelajar tingkatan dua yang mengambil matapelajaran KHB dan data dikumpul menggunakan instrumen borang soal selidik. Dapatan kajian dianalisis menggunakan perisian Winsteps Version 3.69.1.11 dengan pendekatan Model Pengukuran Rasch sepenuhnya. Data dianalisis menggunakan teknik analisis diskriptif dan analisis inferensi iaitu ujian-t bagi menguji hipotesis kajian. Dapatan kajian menunjukkan bahawa amalan pengurusan keselamatan di bengkel diamalkan secara sederhana bagi ketiga-tiga aspek tersebut. Terdapat perbezaan yang signifikan di antara pelajar di bandar dan luar bandar iaitu t=-4.08 dan p=0.000 di mana nilai min ukuranya pula ialah -.19. Beberapa cadangan telah dikemukakan bagi penambahbaikkan seperti mengadakan kempen kesedaran dan menyediakan sudut standard operasi amalan pengurusan keselamatan agar pelajar sentiasa mematuhi peraturan semasa berada di bengkel Kemahiran Hidup

Normal soaking extraction (NSE) of rotenone from Derris elliptica

Derris elliptica or the tuba plant contains rotenone, bio-active compound known that has the potential to be used as bio-pesticide. Bio-pesticide is the best-known alternative bio-pesticide which has the potential to replace the use of conventional pesticides, as it is more environmentally friendly. The main objective of the paper is to obtain the best extraction solvent for optimizing the yield in rotenone extraction. Rotenone was extracted from Derris root using three different parts of roots and three types of solvent in Normal Soaking Extraction (NSE). The types of Derris roots are: (1) Fine root (2) Coarse root (3) Stem. The three types of solvent system are: (1) Acetone 95 % (v/v), (2) Chloroform 99.9 % (v/v) and (3) a mixture of ethanol: H2O (9:1) added with oxalic acid (1mg/ml). The liquid crude extracts were further cleaned up to remove the fine debris of roots. The presence of rotenone was confirmed using qualitative analysis Thin Layer Chromatography (TLC) and thereupon the determination of rotenone content was carried out using High Performance Liquid Chromatography (HPLC). From the results obtained, it was found that the Normal Soaking Extraction (NSE) using acetone 95 % (v/v) was the best method to extract the highest yield of rotenone; 1.14 % (w/w)

Review on automated follicle identification for polycystic ovarian syndrome

Polycystic Ovarian Syndrome (PCOS), is a condition of the ovary consisting numerous follicles. Accurate size and number of follicles detected are crucial for treatment. Hence the diagnosis of this condition is by measuring and calculating the size and number of follicles existed in the ovary. To diagnosis, ultrasound imaging has become an effective tool as it is non invasive, inexpensive and portable. However, the presence of speckle noise in ultrasound imaging has caused an obstruction for manual diagnosis which are high time consumption and often produce errors. Thus, image segmentation for ultrasound imaging is critical to identify follicles for PCOS diagnosis and proper health treatment. This paper presents different methods proposed and applied in automated follicle identification for PCOS diagnosis by previous researchers. In this paper, the methods and performance evaluation are identified and compared. Finally, this paper also provided suggestions in developing methods for future research

The room temperature phosphine-free synthesis of near-infrared emitting HgSe quantum dots

Luminescent mercury selenide (HgSe) quantum dots have been synthesised by a phosphine-free method using oleic acid as a capping agent. The modification of experimental conditions such as temperature resulted in particles of various sizes (15–100 nm) and morphologies not previously seen in HgSe, with emission tuneable between 1000 nm and 1350 nm

Test-retest reliability of knee kinematics measurement during gait with 3D motion analysis system

Restoration of gait symmetry following anterior cruciate ligament reconstruction (ACLR) is crucial to minimize the risk of joint degeneration. To achieve this, it is essential that the chosen measurement method can accurately assess knee kinematics and detect the changes in multi-planes of motion. However to date, limited study is available on repeatability of the multi-planes knee angle measurements particularly among male patients post ACLR. The purpose of this study was to assess the test-retest reliability of knee kinematic measurements using three-dimensional (3D) motion analysis system during gait in post ACLR patients. Eight patients with mean (SD) age 28.89 (4.0) years, 5.82(4.07) months post ACLR were recruited from a tertiary hospital of Kuala Lumpur. All patients undergone two sessions of knee joint angles measurement during gait at four hours interval, for the injured and the non-injured knees. Angles in the sagittal, frontal and transverse planes during initial contact phase of gait that derived from the two measurement sessions were compared.  The results showed high test-retest reliability of the measurement in sagittal and transverse planes; the intraclass correlation coefficient (ICC) was 0.97(95% CI: 0.84-0.99) and 0.96 (95% CI: 0.83-0.99) respectively, and moderate test-retest reliability for the measurements in frontal planes, with ICC 0.44 (95% CI: -0.32-0.86). The study findings suggest that multi-planes knee angle measurements during initial contact phase of gait could reliably be measured using a 3D motion analysis system. Further research may focus on knee kinematics measurements at other phases of gait. Keywords: biomechanics, reliability, walking, knee, anterior cruciate ligament reconstructio

Elements of Community Capacity Building (CCB)for CBET development

Ecotourism is an alternative form of tourism and is usually confused with natural and cultural tourism.CBET is fast becoming a popular biodiversity conservation tool that develops and benefits the local community. Based on the context of conservation theory and practice, Community-Based Ecotourism (CBET) is a form of community-based natural resource management. However, a sustainable CBET development through Community Capacity Building (CCB)programs is not something that it easily achievable. Local community’s capacity varies from one culture to another. It takes a high level of community participation, in order for it to come to a level where the community members themselves are motivated to participate and contribute to the development of the program. This fully qualitative research involved 15 respondents from the community of Kg. Selai, Bekok in Johor, Malaysia. The result show there are five factors that sustained the ecotourism development based for Orang Asli Community in Kg. Selai, namely, existing CBET development, past CBET development, local community participation in planning stage of tourism, local participation in implementation stage of tourism and participation in nature conservation

Effect of membrane performance including fouling on cost optimization in brackish water desalination process

Membrane selection is a crucial step that will affect the economic feasibility of the membrane water treatment process. A comprehensive evaluation consisting of Verberne Cost Model, assessment of membrane performance and fouling propensity, osmotic pressure differential (OPD) and specific energy consumption (SEC) was employed to determine the potential of nanofiltration (NF 270, NF 90 and TS 80) and low pressure reverse osmosis (XLE) membranes to be used in brackish water desalination process. The aim was to save costs by replacing the typical brackish water reverse osmosis (BW 30) membrane. Verberne Cost Model showed that higher flux NF membranes resulted in lower overall costs. However, after assessing the membrane performance, NF 270 and TS 80 were excluded due to their high fouling propensity and their failure to reduce total dissolved solids (TDS) in the solution. Instead, NF 90 membrane which produced water with acceptable TDS and has moderate permeability ended up to be more cost competitive compared to BW 30 membrane, with 17%-21% lower total costs and 13%-17% lower water costs. Apart from this, OPD and SEC were applied to justify the selection of optimal membrane recovery rate based on the water costs calculated. It was determined that the optimal recovery rate was 80% where the SEC and water costs were close to available water treatment plants. Overall, this study showed that the selection of membrane can be carried out by using Verberne Cost Model assisted by assessment of membrane performance and fouling propensity, OPD and SEC

Solvent dependence of the rheological properties in hydrogel magnetorheological plastomer

Chemically crosslinked hydrogel magnetorheological (MR) plastomer (MRP) embedded with carbonyl iron particles (CIPs) exhibits excellent magnetic performance (MR effect) in the presence of external stimuli especially magnetic field. However, oxidation and desiccation in hydrogel MRP due to a large amount of water content as a dispersing phase would limit its usage for long‐term applications, especially in industrial engineering. In this study, different solvents such as dimethyl sulfoxide (DMSO) are also used to prepare polyvinyl alcohol (PVA) hydrogel MRP. Thus, to understand the dynamic viscoelastic properties of hydrogel MRP, three different samples with different solvents: water, DMSO, and their binary mixtures (DMSO/water) were prepared and systematically carried out using the oscillatory shear. The outcomes demonstrate that the PVA hydrogel MRP prepared from precursor gel with water shows the highest MR effect of 15,544% among the PVA hydrogel MRPs. However, the samples exhibit less stability and tend to oxidise after a month. Meanwhile, the samples with binary mixtures (DMSO/water) show an acceptable MR effect of 11,024% with good stability and no CIPs oxidation. Otherwise, the sample with DMSO has the lowest MR effect of 7049% and less stable compared to the binary solvent samples. This confirms that the utilisation of DMSO as a new solvent affects the rheological properties and stability of the samples