1,284 research outputs found
Extending invariant complex structures
We study the problem of extending a complex structure to a given Lie algebra
g, which is firstly defined on an ideal h of g. We consider the next
situations: h is either complex or it is totally real. The next question is to
equip g with an additional structure, such as a (non)-definite metric or a
symplectic structure and to ask either h is non-degenerate, isotropic, etc.
with respect to this structure, by imposing a compatibility assumption. We show
that this implies certain constraints on the algebraic structure of g.
Constructive examples illustrating this situation are shown, in particular
computations in dimension six are given.Comment: 22 pages, plus an Addendu
Central activation of alpha7 nicotinic signaling attenuates lps-induced neuroinflammation and sickness behavior in adult but not in aged animals
We previously reported that lipopolysaccharide (LPS) challenge caused microglial-mediated neuroinflammation and sickness behavior that was amplified in aged mice. As α7 nAChRs are im-plicated in the âCholinergic anti-inflammatory pathwayâ, we aimed to determine how α7 nAChR stimulation modulates microglial phenotype in an LPS-induced neuroinflammation model in adult and aged mice. For this, BALB/c mice were injected intraperitoneally with LPS (0.33 mg/kg) and treated with the α7 nAChR agonist PNU282987, using different administration protocols. LPS challenge reduced body weight and induced lethargy and social withdrawal in adult mice. Peripheral (intraperitoneal) co-administration of the α7 nAChR agonist PNU282987 with LPS, attenuated body weight loss and sickness behavior associated with LPS challenge in adult mice, and reduced microglial activation with suppression of IL-1ÎČ and TNFα mRNA levels. Furthermore, central (intracerebroven-tricular) administration of the α7 nAChR agonist, even 2 h after LPS injection, attenuated the decrease in social exploratory behavior and microglial activation induced by peripheral administration of LPS, although this recovery was not achieved if activation of α7 nAChRs was performed peripherally. Finally, we observed that the positive results of central activation of α7 nAChRs were lost in aged mice. In conclusion, we provide evidence that stimulation of α7 nAChR signaling reduces microglial activation in an in vivo LPS-based model, but this cholinergic-dependent regulation seems to be dysfunctional in microglia of aged mice.This work was supported by the Spanish Ministry of Science, innovation and Universities
Ref. SAF2015-63935-R and Ref. RTI2018-095793-B-I00 and General Council for Research and Innovation of the Community of Madrid and European Structural Funds Ref. B2017/BMDâ3827âNRF24ADCM to M.G.L. Aging studies were supported by an NIA grant (R01-AG-033028) to J.P.G
Kinesins relocalize the chromosomal passenger complex to the midzone for spindle disassembly.
Mitotic spindle disassembly after chromosome separation is as important as spindle assembly, yet the molecular mechanisms for spindle disassembly are unclear. In this study, we investigated how the chromosomal passenger complex (CPC), which contains the Aurora B kinase Ipl1, swiftly concentrates at the spindle midzone in late anaphase, and we researched the role of this dramatic relocalization during spindle disassembly. We showed that the kinesins Kip1 and Kip3 are essential for CPC relocalization. In cells lacking Kip1 and Kip3, spindle disassembly is severely delayed until after contraction of the cytokinetic ring. Purified Kip1 and Kip3 interact directly with the CPC and recruit it to microtubules in vitro, and single-molecule experiments showed that the CPC diffuses dynamically on microtubules but that diffusion stops when the CPC encounters a Kip1 molecule. We propose that Kip1 and Kip3 trap the CPC at the spindle midzone in late anaphase to ensure timely spindle disassembly
Extinction times in the subcritical stochastic SIS logistic epidemic
Many real epidemics of an infectious disease are not straightforwardly super-
or sub-critical, and the understanding of epidemic models that exhibit such
complexity has been identified as a priority for theoretical work. We provide
insights into the near-critical regime by considering the stochastic SIS
logistic epidemic, a well-known birth-and-death chain used to model the spread
of an epidemic within a population of a given size . We study the behaviour
of the process as the population size tends to infinity. Our results cover
the entire subcritical regime, including the "barely subcritical" regime, where
the recovery rate exceeds the infection rate by an amount that tends to 0 as but more slowly than . We derive precise asymptotics for
the distribution of the extinction time and the total number of cases
throughout the subcritical regime, give a detailed description of the course of
the epidemic, and compare to numerical results for a range of parameter values.
We hypothesise that features of the course of the epidemic will be seen in a
wide class of other epidemic models, and we use real data to provide some
tentative and preliminary support for this theory.Comment: Revised; 34 pages; 6 figure
Lack of microbiological concordance between bone and non-bone specimens in chronic osteomyelitis: an observational study
BACKGROUND: Prognosis of chronic osteomyelitis depends heavily on proper identification and treatment of the bone-infecting organism. Current knowledge on selecting the best specimen for culture is confusing, and many consider that non-bone specimens are suitable to replace bone cultures. This paper compares the microbiology of non-bone specimens with bone cultures, taking the last as the diagnostic gold standard. METHODS: Retrospective observational analysis of 50 patients with bacterial chronic osteomyelitis in a 750-bed University-based hospital. RESULTS: Concordance between both specimens for all etiologic agents was 28%, for Staphylococcus aureus 38%, and for organisms other than S. aureus 19%. The culture of non-bone specimens to identify the causative organisms in chronic osteomyelitis produced 52% false negatives and 36% false positives when compared against bone cultures. CONCLUSIONS: Diagnosis and therapy of chronic osteomyelitis cannot be guided by cultures of non-bone specimens because their microbiology is substantially different to the microbiology of the bone
HIPK2 and extrachromosomal histone H2B are separately recruited by Aurora-B for cytokinesis
Cytokinesis, the final phase of cell division, is necessary to form two distinct daughter cells with correct distribution of genomic and cytoplasmic materials. Its failure provokes genetically unstable states, such as tetraploidization and polyploidization, which can contribute to tumorigenesis. Aurora-B kinase controls multiple cytokinetic events, from chromosome condensation to abscission when the midbody is severed. We have previously shown that HIPK2, a kinase involved in DNA damage response and development, localizes at the midbody and contributes to abscission by phosphorylating extrachromosomal histone H2B at Ser14. Of relevance, HIPK2-defective cells do not phosphorylate H2B and do not successfully complete cytokinesis leading to accumulation of binucleated cells, chromosomal instability, and increased tumorigenicity. However, how HIPK2 and H2B are recruited to the midbody during cytokinesis is still unknown. Here, we show that regardless of their direct (H2B) and indirect (HIPK2) binding of chromosomal DNA, both H2B and HIPK2 localize at the midbody independently of nucleic acids. Instead, by using mitotic kinase-specific inhibitors in a spatio-temporal regulated manner, we found that Aurora-B kinase activity is required to recruit both HIPK2 and H2B to the midbody. Molecular characterization showed that Aurora-B directly binds and phosphorylates H2B at Ser32 while indirectly recruits HIPK2 through the central spindle components MgcRacGAP and PRC1. Thus, among different cytokinetic functions, Aurora-B separately recruits HIPK2 and H2B to the midbody and these activities contribute to faithful cytokinesis
Imaging Jupiter's radiation belts down to 127 MHz with LOFAR
Context. Observing Jupiter's synchrotron emission from the Earth remains
today the sole method to scrutinize the distribution and dynamical behavior of
the ultra energetic electrons magnetically trapped around the planet (because
in-situ particle data are limited in the inner magnetosphere). Aims. We perform
the first resolved and low-frequency imaging of the synchrotron emission with
LOFAR at 127 MHz. The radiation comes from low energy electrons (~1-30 MeV)
which map a broad region of Jupiter's inner magnetosphere. Methods (see article
for complete abstract) Results. The first resolved images of Jupiter's
radiation belts at 127-172 MHz are obtained along with total integrated flux
densities. They are compared with previous observations at higher frequencies
and show a larger extent of the synchrotron emission source (>=4 ). The
asymmetry and the dynamic of east-west emission peaks are measured and the
presence of a hot spot at lambda_III=230 {\deg} 25 {\deg}. Spectral flux
density measurements are on the low side of previous (unresolved) ones,
suggesting a low-frequency turnover and/or time variations of the emission
spectrum. Conclusions. LOFAR is a powerful and flexible planetary imager. The
observations at 127 MHz depict an extended emission up to ~4-5 planetary radii.
The similarities with high frequency results reinforce the conclusion that: i)
the magnetic field morphology primarily shapes the brightness distribution of
the emission and ii) the radiating electrons are likely radially and
latitudinally distributed inside about 2 . Nonetheless, the larger extent
of the brightness combined with the overall lower flux density, yields new
information on Jupiter's electron distribution, that may shed light on the
origin and mode of transport of these particles.Comment: 10 pages, 12 figures, accepted for publication in A&A (27/11/2015) -
abstract edited because of limited character
LOFAR Sparse Image Reconstruction
Context. The LOw Frequency ARray (LOFAR) radio telescope is a giant digital
phased array interferometer with multiple antennas distributed in Europe. It
provides discrete sets of Fourier components of the sky brightness. Recovering
the original brightness distribution with aperture synthesis forms an inverse
problem that can be solved by various deconvolution and minimization methods
Aims. Recent papers have established a clear link between the discrete nature
of radio interferometry measurement and the "compressed sensing" (CS) theory,
which supports sparse reconstruction methods to form an image from the measured
visibilities. Empowered by proximal theory, CS offers a sound framework for
efficient global minimization and sparse data representation using fast
algorithms. Combined with instrumental direction-dependent effects (DDE) in the
scope of a real instrument, we developed and validated a new method based on
this framework Methods. We implemented a sparse reconstruction method in the
standard LOFAR imaging tool and compared the photometric and resolution
performance of this new imager with that of CLEAN-based methods (CLEAN and
MS-CLEAN) with simulated and real LOFAR data Results. We show that i) sparse
reconstruction performs as well as CLEAN in recovering the flux of point
sources; ii) performs much better on extended objects (the root mean square
error is reduced by a factor of up to 10); and iii) provides a solution with an
effective angular resolution 2-3 times better than the CLEAN images.
Conclusions. Sparse recovery gives a correct photometry on high dynamic and
wide-field images and improved realistic structures of extended sources (of
simulated and real LOFAR datasets). This sparse reconstruction method is
compatible with modern interferometric imagers that handle DDE corrections (A-
and W-projections) required for current and future instruments such as LOFAR
and SKAComment: Published in A&A, 19 pages, 9 figure
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