181 research outputs found
Molecular Chemical Engines: Pseudo-Static Processes and the Mechanism of Energy Transduction
We propose a simple theoretical model for a molecular chemical engine that
catalyzes a chemical reaction and converts the free energy released by the
reaction into mechanical work. Binding and unbinding processes of reactant and
product molecules to and from the engine are explicitly taken into account. The
work delivered by the engine is calculated analytically for infinitely slow
(``pseudo-static'') processes, which can be reversible (quasi-static) or
irreversible, controlled by an external agent. It is shown that the work larger
than the maximum value limited by the second law of thermodynamics can be
obtained in a single cycle of operation by chance, although the statistical
average of the work never exceeds this limit and the maximum work is delivered
if the process is reversible. The mechanism of the energy transductionis also
discussed.Comment: 8 pages, 3 figues, submitted to J. Phys. Soc. Jp
In-beam Ξ³-ray Spectroscopy Studies of Medium-spin States in the Odd-odd Nucleus \u3csup\u3e186\u3c/sup\u3eRe
Excited states in 186Re with spins up to J=12β were investigated in two separate experiments using 186W(d,2n) reactions at beam energies of 12.5 and 14.5 MeV. Two- and threefold Ξ³-ray coincidence data were collected using the CAESAR and CAGRA spectrometers, respectively, each composed of Compton-suppressed high-purity germanium detectors. Analysis of the data revealed rotational bands built on several two-quasiparticle intrinsic states, including a long-lived KΟ=(8+) isomer. Configuration assignments were supported by an analysis of in-band properties, such as |gKβgR| values. The excitation energies of the observed intrinsic states were compared with results from multi-quasiparticle blocking calculations, based on the Lipkin-Nogami pairing approach, that included contributions from the residual proton-neutron interactions. Abstract Β©2017 American Physical Societ
Single-molecule experiments in biological physics: methods and applications
I review single-molecule experiments (SME) in biological physics. Recent
technological developments have provided the tools to design and build
scientific instruments of high enough sensitivity and precision to manipulate
and visualize individual molecules and measure microscopic forces. Using SME it
is possible to: manipulate molecules one at a time and measure distributions
describing molecular properties; characterize the kinetics of biomolecular
reactions and; detect molecular intermediates. SME provide the additional
information about thermodynamics and kinetics of biomolecular processes. This
complements information obtained in traditional bulk assays. In SME it is also
possible to measure small energies and detect large Brownian deviations in
biomolecular reactions, thereby offering new methods and systems to scrutinize
the basic foundations of statistical mechanics. This review is written at a
very introductory level emphasizing the importance of SME to scientists
interested in knowing the common playground of ideas and the interdisciplinary
topics accessible by these techniques. The review discusses SME from an
experimental perspective, first exposing the most common experimental
methodologies and later presenting various molecular systems where such
techniques have been applied. I briefly discuss experimental techniques such as
atomic-force microscopy (AFM), laser optical tweezers (LOT), magnetic tweezers
(MT), biomembrane force probe (BFP) and single-molecule fluorescence (SMF). I
then present several applications of SME to the study of nucleic acids (DNA,
RNA and DNA condensation), proteins (protein-protein interactions, protein
folding and molecular motors). Finally, I discuss applications of SME to the
study of the nonequilibrium thermodynamics of small systems and the
experimental verification of fluctuation theorems. I conclude with a discussion
of open questions and future perspectives.Comment: Latex, 60 pages, 12 figures, Topical Review for J. Phys. C (Cond.
Matt
Unexpected distribution of Ξ½1f7/2 strength in Ca 49
The calcium isotopes have emerged as a critical testing ground for new microscopically derived shell-model interactions, and a great deal of experimental and theoretical focus has been directed toward this region. We investigate the relative spectroscopic strengths associated with 1f7/2 neutron hole states in Ca47,49 following one-neutron knockout reactions from Ca48,50. The observed reduction of strength populating the 7/21- state in Ca49, as compared to Ca47, is inconsistent with shell-model calculations using both phenomenological interactions such as GXPF1, and interactions derived from microscopically based two- and three-nucleon forces. The result suggests a fragmentation of the l=3 strength to higher-lying states as suggested by the microscopic calculations, but the observed magnitude of the reduction is not reproduced in any shell-model description
Serum Biomarkers of Myocardial Remodeling and Coronary Dysfunction in Early Stages of Hypertrophic Cardiomyopathy in the Young
HIV-1 Tat protein directly induces mitochondrial membrane permeabilization and inactivates cytochrome c oxidase
The Trans-activator protein (Tat) of human immunodeficiency virus (HIV)
is a pleiotropic protein involved in different aspects of AIDS pathogenesis. As
a number of viral proteins Tat is suspected to disturb mitochondrial function.
We prepared pure synthetic full-length Tat by native chemical ligation (NCL),
and Tat peptides, to evaluate their direct effects on isolated mitochondria.
Submicromolar doses of synthetic Tat cause a rapid dissipation of the
mitochondrial transmembrane potential (ΞΞ¨m) as well as
cytochrome c release in mitochondria isolated from mouse liver, heart,
and brain. Accordingly, Tat decreases substrate oxidation by mitochondria
isolated from these tissues, with oxygen uptake being initially restored by
adding cytochrome c. The anion-channel inhibitor
4,4β²-diisothiocyanostilbene-2,2β²-disulfonic acid (DIDS) protects
isolated mitochondria against Tat-induced mitochondrial membrane
permeabilization (MMP), whereas ruthenium red, a ryanodine receptor blocker,
does not. Pharmacologic inhibitors of the permeability transition pore,
Bax/Bak inhibitors, and recombinant Bcl-2 and Bcl-XL proteins do not reduce
Tat-induced MMP. We finally observed that Tat inhibits cytochrome c
oxidase (COX) activity in disrupted mitochondria isolated from liver, heart, and
brain of both mouse and human samples, making it the first described viral
protein to be a potential COX inhibitor
Guidelines for the management of biliary tract and ampullary carcinomas: surgical treatment
The only curative treatment in biliary tract cancer is surgical treatment. Therefore, the suitability of curative resection should be investigated in the first place. In the presence of metastasis to the liver, lung, peritoneum, or distant lymph nodes, curative resection is not suitable. No definite consensus has been reached on local extension factors and curability. Measures of hepatic functional reserve in the jaundiced liver include future liver remnant volume and the indocyanine green (ICG) clearance test. Preoperative portal vein embolization may be considered in patients in whom right hepatectomy or more, or hepatectomy with a resection rate exceeding 50%β60% is planned. Postoperative complications and surgery-related mortality may be reduced with the use of portal vein embolization. Although hepatectomy and/or pancreaticoduodenectomy are preferable for the curative resection of bile duct cancer, extrahepatic bile duct resection alone is also considered in patients for whom it is judged that curative resection would be achieved after a strict diagnosis of its local extension. Also, combined caudate lobe resection is recommended for hilar cholangiocarcinoma. Because the prognosis of patients treated with combined portal vein resection is significantly better than that of unresected patients, combined portal vein resection may be carried out. Prognostic factors after resection for bile duct cancer include positive surgical margins, especially in the ductal stump; lymph node metastasis; perineural invasion; and combined vascular resection due to portal vein and/or hepatic artery invasion. For patients with suspected gallbladder cancer, laparoscopic cholecystectomy is not recommended, and open cholecystectomy should be performed as a rule. When gallbladder cancer invading the subserosal layer or deeper has been detected after simple cholecystectomy, additional resection should be considered. Prognostic factors after resection for gallbladder cancer include the depth of mural invasion; lymph node metastasis; extramural extension, especially into the hepatoduodenal ligament; perineural invasion; and the degree of curability. Pancreaticoduodenectomy is indicated for ampullary carcinoma, and limited operation is also indicated for carcinoma in adenoma. The prognostic factors after resection for ampullary carcinoma include lymph node metastasis, pancreatic invasion, and perineural invasion
Anaerobic growth and potential for amino acid production by nitrate respiration in Corynebacterium glutamicum
Diagnosis of biliary tract and ampullary carcinomas
Diagnostic methods for biliary tract carcinoma and the efficacy of these methods are discussed. Neither definite methods for early diagnosis nor specific markers are available in this disease. When this disease is suspected on the basis of clinical symptoms and risk factors, hemato-biochemical examination and abdominal ultrasonography are performed and, where appropriate, enhanced computed tomography (CT) and/or magnetic resonance cholangiopancreatography (MRCP) is carried out. Diagnoses of extrahepatic bile duct cancer and ampullary carcinoma are often made based on the presence of obstructive jaundice. Although rare, abdominal pain and pyrexia, as well as abnormal findings of the hepatobiliary system detected by hemato-biochemical examination, serve as a clue to making a diagnosis of these diseases. On the other hand, the early diagnosis of gallbladder cancer is scarcely possible on the basis of clinical symptoms, so when this cancer is found with the onset of abdominal pain and jaundice, it is already advanced at the time of detection, thus making a cure difficult. When gallbladder cancer is suspected, enhanced CT is carried out. Multidetector computed tomography (MDCT), in particular β one of the methods of enhanced CT β is useful for decision of surgical criteria, because MDCT shows findings such as localization and extension of the tumor, and the presence or absence of remote metastasis. Procedures such as magnetic resonance imaging, endoscopic ultrasonography, bile duct biopsy, and cholangioscopy should be carried out taking into account indications for these procedures in individual patients. However, direct biliary tract imaging is necessary for making a precise diagnosis of the horizontal extension of bile duct cancer
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