Acute renal failure in four Comrades Marathon runners ingesting the same electrolyte supplement: Coincidence or causation?

Objectives. To evaluate common factors associated with the development of acute renal failure (ARF) in Comrades Marathon runners.Methods. This was a retrospective case series of 4 runners hospitalised post-race with ARF in the 89 km 2010 Comrades Marathon. The outcome measures were incidence of analgesic use, levels of creatine  phosphokinase (CPK) and degree of electrolyte supplementation (sodium, potassium, calcium and magnesium).Results. The prevalence of ARF was 1/4 125 runners. They presented with rhabdomyolysis (mean admission CPK of 36 294 IU) and hyponatraemia (mean admission blood sodium level of 133 mEq/l). All had ingested an analgesic during the run (3 ingested a non-steroidal anti-inflammatory  drug) and the same readily available anti-cramp electrolyte supplement.  The average amount of supplemental sodium (452 mg), potassium (393 mg), calcium (330 mg) and magnesium (154 mg) ingested via this  particular electrolyte supplement before and during the run did not exceedthe recommended upper limits of daily intake. Three of the runners were Comrades Marathon novices.Conclusions. There is a continuing need to clarify the specific cluster variants that cause ARF in Comrades Marathon runners, as the risk factors appear to have evolved since the first case was described over 40 years ago.S Afr Med J 2011;101:876-878

Bactericidal action of positive and negative ions in air

In recent years there has been renewed interest in the use of air ionisers to control of the spread of airborne infection. One characteristic of air ions which has been widely reported is their apparent biocidal action. However, whilst the body of evidence suggests a biocidal effect in the presence of air ions the physical and biological mechanisms involved remain unclear. In particular, it is not clear which of several possible mechanisms of electrical origin (i.e. the action of the ions, the production of ozone, or the action of the electric field) are responsible for cell death. A study was therefore undertaken to clarify this issue and to determine the physical mechanisms associated with microbial cell death. In the study seven bacterial species (Staphylococcus aureus, Mycobacterium parafortuitum, Pseudomonas aeruginosa, Acinetobacter baumanii, Burkholderia cenocepacia, Bacillus subtilis and Serratia marcescens) were exposed to both positive and negative ions in the presence of air. In order to distinguish between effects arising from: (i) the action of the air ions; (ii) the action of the electric field, and (iii) the action of ozone, two interventions were made. The first intervention involved placing a thin mica sheet between the ionisation source and the bacteria, directly over the agar plates. This intervention, while leaving the electric field unaltered, prevented the air ions from reaching the microbial samples. In addition, the mica plate prevented ozone produced from reaching the bacteria. The second intervention involved placing an earthed wire mesh directly above the agar plates. This prevented both the electric field and the air ions from impacting on the bacteria, while allowing any ozone present to reach the agar plate. With the exception of Mycobacterium parafortuitum, the principal cause of cell death amongst the bacteria studied was exposure to ozone, with electroporation playing a secondary role. However in the case of Mycobacterium parafortuitum, electroporation resulting from exposure to the electric field appears to have been the principal cause of cell inactivation. The results of the study suggest that the bactericidal action attributed to negative air ions by previous researchers may have been overestimated

Probing the interfacial and sub-surface structure of Si/Si1 – xGex multilayers

The ability to determine structural and compositional information from the sub-surface region of a semiconductor material has been demonstrated using a new time-of-flight medium energy ion scattering spectroscopy (ToF-MEISS) system. A series of silicon–silicon/germanium (Si/Si1 – xGex) heterostructure and multilayer samples, grown using both solid source molecular beam epitaxy (MBE) and gas source chemical vapor deposition (CVD) on Si(100) substrates, have been investigated. These data indicate that each individual layer of Si1 – xGex (x ~ 0.22) in both two- and three-period samples, can be uniquely identified with a resolution of approximately 3 nm. A comparison of MBE and CVD grown samples has also been made using layers with similar structures and composition. The total Ge content of each sample was confirmed using conventional Rutherford backscattering spectrometry

Multi-objective variational curves

Riemannian cubics in tension are critical points of the linear combination of two objective functionals, namely the squared norms of the velocity and acceleration of a curve on a Riemannian manifold. We view this variational problem of finding a curve as a multi-objective optimization problem and construct the Pareto fronts for some given instances where the manifold is a sphere and where the manifold is a torus. The Pareto front for the curves on the torus turns out to be particularly interesting: the front is disconnected and it reveals two distinct Riemannian cubics with the same boundary data, which is the first known nontrivial instance of this kind. We also discuss some convexity conditions involving the Pareto fronts for curves on general Riemannian manifolds

Invariant higher-order variational problems II

Motivated by applications in computational anatomy, we consider a second-order problem in the calculus of variations on object manifolds that are acted upon by Lie groups of smooth invertible transformations. This problem leads to solution curves known as Riemannian cubics on object manifolds that are endowed with normal metrics. The prime examples of such object manifolds are the symmetric spaces. We characterize the class of cubics on object manifolds that can be lifted horizontally to cubics on the group of transformations. Conversely, we show that certain types of non-horizontal geodesics on the group of transformations project to cubics. Finally, we apply second-order Lagrange--Poincar\'e reduction to the problem of Riemannian cubics on the group of transformations. This leads to a reduced form of the equations that reveals the obstruction for the projection of a cubic on a transformation group to again be a cubic on its object manifold.Comment: 40 pages, 1 figure. First version -- comments welcome

Why We Need a Ruminant Revolution: Combating Malnutrition and Metabolic Illnesses to Enable Sustainable Development

Animal source foods (ASF) are essential for proper human development and function. Livestock in general, and ruminants in particular, are essential components of our sustainable global food systems. Of significant worldwide impact, diets with higher-than-recommended levels of ASF can correct the symptoms of metabolic illnesses, offering hope in arresting the current worldwide epidemic of diabetes and other metabolic diseases. Most dietary policy and recommendations are based on the ill-founded belief that plant-based, high-carbohydrate diets are “healthy.” High-quality scientific evidence does not support the belief that vegetarian diets are healthier than omnivorous or animal-based diets. A Therapeutic Carbohydrate Reduction (TCR) lifestyle approach has demonstrated its efficacy in reversing Insulin Resistance (IR) and the non-communicable diseases associated with, or caused, by it. True sustainability is a multifaceted topic consisting of societal, economic, and ecological aspects. The enormous suffering and financial costs of chronic illness must be acknowledged. The production of high-quality animal protein and animal fat by ruminants from feed resources humans cannot directly utilize will be fundamental to feeding a growing population. This essential food production can preserve and enhance the diverse environments where it takes place. We need a revolution in our thinking of what constitutes a healthy diet, of what causes chronic illness, of the vital role that animal product play in the human diet, and the essential nature of ruminant animal agriculture in meeting humanity’s needs. This will mean overthrowing established policies and institutions, and confronting vested belief systems. We’ll need an effort, analogous to the Green Revolution, to develop and deploy the knowledge and technology necessary to meet the needs of the mid-21st Century world

Avoid adding insult to injury – correct management of sick female endurance athletes

Objectives. To evaluate the efficacy of Ringer’s lactate, isotonic saline and hypertonic saline on the clinical and biochemical recovery of athletes with exercise-associated hyponatraemic encephalopathy caused by fluid overload.Methods. We retrospectively reviewed serial blood sodium concentrations (Na+) and qualitative signs of recovery and time to recovery in two healthy menstruant females hospitalised with dilutional exercise-associated hyponatraemic encephalopathy after withdrawal from the 2011 Comrades Marathon (89 km) and Argus Cycle Tour (109 km).Results. Improvements in blood Na+ did not occur with intravenous administration of Ringer’s lactate solution, but did occur with administration of isotonic and hypertonic saline. Qualitative improvements in mental status were not quantitatively related to the biochemical value of blood Na+ or subsequent return to normonatraemia.Conclusions. Hyponatraemia should be suspected in all female athletes presenting to the medical area of endurance races with vomiting, altered mental status and a history of high fluid intake. If a diagnosis of exercise-associated hyponatraemia with cerebral encephalopathy is confirmed, the treatment of choice is administration of an intravenous bolus of hypertonic saline. Administration of Ringer’s lactate should be discouraged, as this does not correct Na+ and appears to delay recovery

Differential localization and expression of complement in a rat model of motor neuron disease

ComBio is the major ASBMB conference held each year, in association with other organisations. This page has links to past, current and future ComBio meetings. Further information and links will be placed online as they become available

Coexistence of ferromagnetism and superconductivity in the hybrid ruthenate-cuprate compound RuSr_2GdCu_2O_8 studied by muon spin rotation (\mu SR) and DC-magnetization

We have investigated the magnetic and the superconducting properties of the hybrid ruthenate-cuprate compound RuSr_{2}GdCu_{2}O_{8} by means of zero-field muon spin rotation- (ZF-$\mu$SR) and DC magnetization measurements. The DC-magnetisation data establish that this material exhibits ferromagnetic order of the Ru-moments ($\mu (Ru) \approx 1 \mu_{B}$) below T_{Curie} = 133 K and becomes superconducting at a much lower temperature T_c = 16 K. The ZF-$\mu$SR experiments indicate that the ferromagnetic phase is homogeneous on a microscopic scale and accounts for most of the sample volume. They also suggest that the magnetic order is not significantly modified at the onset of superconductivity.Comment: improved version submitted to Phys. Rev.