PABRA means partnership: Transforming agriculture in Africa together

For two decades, the Pan-Africa Bean Research Alliance (PABRA), a partnership developed and facilitated by the International Center for Tropical Agriculture (CIAT) – a CGIAR Research Center – has fostered a pan-African research and development partnership to strengthen the common bean value chain. Common beans are important food legumes and contribute to the food and nutrition security, income generation and enhances production systems in over 32 countries in sub-Saharan Africa. To achieve food and nutrition security, Africa must participate, promote regional, continental, and global markets, and strengthen key value chains. Our policies need to be deliberately tailored, made conducive and supportive so as to take advantage of opportunities offered by technological development and innovations. Enhancing capacities of different stakeholder cannot be emphasized. With a changing climate, Africa needs to be prepared with mitigation strategies, plans, policies, tools and actions that enhance resilience of our production systems and communities

Inheritance of resistance to common bacterial blight in four selected common bean (\u3ci\u3ePhaseolus vulgaris\u3c/i\u3e L.) genotypes

Common bacterial blight (CBB) is the most serious bacterial disease of common bean in Uganda. It causes severe yield losses of up to 62%. Genetic resistance is the most effective option for controlling CBB in smallholder common bean production systems. This study was carried out to determine the inheritance pattern of CBB resistance in leaf and pod of four new resistance sources. The four resistant and four susceptible genotypes were crossed in a half-diallel mating design. F1 individuals were advanced to F2 and evaluated with the parents, in a randomized complete block design replicated twice. Combining ability analysis was performed according to Griffing\u27s (1956) method IV and model 1 using Genstat 12th. General combining ability effects were significant whereas specific combining ability was not suggesting that resistance to CBB in leaf and pod was primarily controlled by additive genes effects. The estimated narrow sense coefficient of genetic determination was moderately high (0.65) for the resistance in leaf and high (0.83) for resistance in pod suggesting that early-generation selection would be effective. Baker’s ratio estimates were relatively high for resistance in leaf (0.79) and pod (0.9) suggesting that hybrids’ performance can be predicted based on the parents’ general combining ability (GCA) effects

Effect of intermittent drought on phenotypic traits of F5 RIL Andean intra-gene cross population (BRB 191 X SEQ 1027) of common bean

Drought is a major constraint to common bean ( Phaseolus vulgaris L.) production in East Africa, where irrigation for the crop is very uncommon. The objective of this study was to identify drought tolerant lines and phenotypic traits underlying drought tolerance among 128 F5 recombinant inbred lines (RILs), derived from intra gene pool population, between drought tolerant BRB 191 (source of bc-3) and SEQ 1027. The population was evaluated with eight experimental checks that included BAT 477, CAL 96, DAB 441, DAB 494, and Diacol Calima, NABE 4, SCR 9 and SEQ 1003. A total of 20 phenological, morphological and physiological shoot traits were evaluated, under drought and non-stress conditions, in the field for 2 years (2014 and 2015) at Kawanda in Uganda. New sources of drought tolerance, and previously identified sources of drought tolerance in common bean (BAT 477, DAB 441 and DAB 494), were confirmed based on their superior geometric means and low drought susceptibility. Drought stress in the field significantly affected all measured traits, except harvest index and stem dry weight reduction (P<0.001). Drought significantly reduced yield, yield components and pod harvest index (P<0.01). However, chlorophyll content, canopy temperature, stem dry mass reduction, and 100 seed weight remained stable under season by genotype by water regime treatment interactions (S x G x T). The stability of these traits highlighted their usefulness in selecting for drought tolerance across different environments. Furthermore, pod partitioning index (PPI), harvest index (HI), chlorophyll content and stem dry weight reduction also remained stable under G x T effects. Significant correlations (P<0.001) were maintained between HI and PPI with seed yield under drought stress in field conditions, indicating that photosynthate remobilisation increases yield under drought stress conditions.La s\ue9cheresse est une contrainte major \ue0 la production du haricot commun ( Phaseolus vulgaris L.) en Afrique de l\u2019Est, o\uf9 l\u2019irrigation pour la culture est tr\ue8s rare. L\u2019objectif de cette \ue9tude a \ue9t\ue9 d\u2019identifier les lign\ue9es tol\ue9rantes \ue0 la s\ue9cheresse et les traits ph\ue9notypiques contr\uf4lant la tol\ue9rance \ue0 la s\ue9cheresse parmi les 128 F5 de lign\ue9es consanguines recombinantes, d\ue9riv\ue9es d\u2019une population intra-g\ue8ne de la r\ue9gion andine, entre la lign\ue9e tol\ue9rante \ue0 la s\ue9cheresse BRB191 (source du bc-3) et SEQ 1027. La population a \ue9t\ue9 \ue9valu\ue9e avec huit contr\uf4les exp\ue9rimentaux comprenant BAT 477, CAL 96, DAB 441, DAB 494, et Diacol calima, NABE 4, SCR 9 et SEQ 1003. Un total de 20 traits phr\ue9nologiques, morphologiques et physiologiques a \ue9t\ue9 \ue9valu\ue9, sous les conditions de stress hydrique, dans le champ pour 2 ans (2014 et 2015) \ue0 Kwanda en Uganda. De nouvelles sources de tol\ue9rance, et des sources de tol\ue9rance pr\ue9c\ue9demment identifi\ue9es dans le haricot commun (BAT 477, DAB 441 et DAB 494), ont \ue9t\ue9 confirm\ue9es sur la base des moyens de sup\ue9riorit\ue9 g\ue9om\ue9triques et de susceptibilit\ue9 de faible s\ue8cheresse. Le stress hydrique dans le champ a significativement affect\ue9 tous les traits mesur\ue9s, \ue0 l\u2019exception de l\u2019indice de r\ue9colte et la r\ue9duction du poids sec de la tige (P<0,001). La s\ue9cheresse a significativement r\ue9duit le rendement, les composantes du rendement et l\u2019indice de la r\ue9colte de la gousse (P<0.01). Toutefois, la temp\ue9rature de la canop\ue9e, la r\ue9duction du poids sec de la tige, et le poids de 100 graines sont rest\ue9s stables sous le traitement de l\u2019interaction entre la saison x g\ue9notype x r\ue9gime d\u2019eau de m\ueame que l\u2019interaction entre le g\ue9notype x le traitement (G X T) (P<0,001). La stabilit\ue9 de ces traits a montr\ue9 leur utilit\ue9 dans la s\ue9lection pour la tol\ue9rance \ue0 la s\ue9cheresse \ue0 travers les diff\ue9rents environnements. De plus, l\u2019indice du partitionnement de la gousse (PPI), l\u2019indice de r\ue9colte (HI), la teneur en chlorophylle et la r\ue9duction du poids sec de la tige sont rest\ue9s \ue9galement stables sous les effets de G x T. Des corr\ue9lations significatives (P<0,001) ont \ue9t\ue9 maintenues entre HI et PPI avec le rendement en graine sous le stress hydrique dans les conditions de champ, indiquant la viabilit\ue9 de la remobilisation de la photosynthate dans l\u2019augmentation du rendement potentiel sous les conditions de stress hydrique

AGRONOMIC QUALITIES OF GENETIC PYRAMIDS OF COMMON BEAN DEVELOPED FOR MULTIPLE-DISEASE-RESISTANCE

Multiple co-infections by different pathogens on common bean ( Phaseolus vulgaris L.) affect its productivity and cause complete crop loss in susceptible varieties. Therefore, gene pyramiding using marker assisted selection (MAS) and backcrossing, provide alternative cost-effective control measures to bean diseases. However, in the process of developing pyramids, linkage drags were likely to affect the qualities of progeny lines, hence, special attention was paid to this situation. The objective of this study was thus to assess the agronomic qualities of advanced genetic pyramids developed from a four-way cross for multiple disease resistance. The disease resistance genes (R) pyramided from four parents were: Co42 and Co-5 from G2333; Phg-2 from MEX54; Pythium ultimum Dennis from MLB49-89A and I & bc3 from MCM5001. The progeny lines were planted in an incomplete block design, and replicated thrice for two seasons (2015A and 2015B) in fields at CIAT, Kawanda in Uganda. Agronomic traits were highly heritable (0.6), except number of pods per plant (< 0.3). Backcrossing generated high-yielding bean lines, with 270 - 290 seed per plant and early maturity (95-100 days). Nine superior lines with desirable qualities, such as earliness (95 days), high seed rate (290 seeds per plant), and climbing ability, were obtained. Pyramiding R genes did not affect yield traits, except time to flowering and number of flower buds per plant due to transgressive segregation.Les co-infections multiples par de diff\ue9rents pathog\ue8nes sur le haricot commun ( Phaseolus vulgaris L.) affectent sa productivit\ue9 et causent la perte totale des vari\ue9t\ue9s susceptibles de la culture. Par cons\ue9quent, la pyramide des g\ue8nes en utilisant la s\ue9lection assist\ue9e par des marqueurs (MAS) et le r\ue9trocroisement, fournissent des mesures alternatives de contr\uf4le moins ch\ue8res des maladies du haricot. N\ue9anmoins, dans le processus du d\ue9veloppement des pyramides, les poids des liaisons affectent probablement les qualit\ue9s des lign\ue9es de prog\ue9nitures, de ce fait, une attention particuli\ue8re \ue9tait port\ue9e \ue0 cette situation. L\u2019objectif de cette \ue9tude \ue9tait d\u2019\ue9valuer les qualit\ue9s agronomiques des pyramides g\ue9n\ue9tiques avanc\ue9es d\ue9velopp\ue9es d\u2019un croisement de quatre parents pour la r\ue9sistance aux maladies multiples. Les g\ue8nes pyramid\ue9s de r\ue9sistance (R) \ue0 la maladie de quatre parents \ue9taient\ua0: Co42 et Co-5 de G2333; Phg-2 de MEX54; Pythium ultimum Dennis de MLB49-89A et I & bc3 de MCM5001. Les lign\ue9es de prog\ue9nitures \ue9taient plant\ue9es en arrangement de block incomplet et r\ue9pliqu\ue9 trois fois pendant deux saisons (2015A et 2015B) dans les champs \ue0 CIAT, Kawanda en Ouganda. Les traits agronomiques \ue9taient hautement h\ue9ritables (0,6), \ue0 l\u2019exception du nombre de gousses par plant (<0.3). Le r\ue9trocroisement a g\ue9n\ue9r\ue9 des lign\ue9es d\u2019haricot \ue0 haut rendement, avec 270-290 graines par plant et \ue0 maturit\ue9 pr\ue9coce (95-100 jours). Neuf lign\ue9es sup\ue9rieures avec des qualit\ue9s d\ue9sirables, telles que la pr\ue9cocit\ue9 (95 jours), taux de graines \ue9lev\ue9s (290 graines par plant) et l\u2019habilit\ue9 grimpante, \ue9taient obtenues. La pyramide des g\ue8nes R n\u2019avait pas affect\ue9 les traits li\ue9s au rendement, \ue0 l\u2019exception de la p\ue9riode de floraison et le nombre de bougeons de fleurs par plant d\ufb \ue0 la s\ue9gr\ue9gation transgressive

Redesigning crop varieties to win the race between climate change and food security

Climate change poses daunting challenges to agricultural production and food security. Rising temperatures, shifting weather patterns, and more frequent extreme events have already demonstrated their effects on local, regional, and global agricultural systems. Crop varieties that withstand climate-related stresses and are suitable for cultivation in innovative cropping systems will be crucial to maximize risk avoidance, productivity, and profitability under climate-changed environments. We surveyed 588 expert stakeholders to predict current and novel traits that may be essential for future pearl millet, sorghum, maize, groundnut, cowpea, and common bean varieties, particularly in sub-Saharan Africa. We then review the current progress and prospects for breeding three prioritized future-essential traits for each of these crops. Experts predict that most current breeding priorities will remain important, but that rates of genetic gain must increase to keep pace with climate challenges and consumer demands. Importantly, the predicted future-essential traits include innovative breeding targets that must also be prioritized; for example, (1) optimized rhizosphere microbiome, with benefits for P, N, and water use efficiency, (2) optimized performance across or in specific cropping systems, (3) lower nighttime respiration, (4) improved stover quality, and (5) increased early vigor. We further discuss cutting-edge tools and approaches to discover, validate, and incorporate novel genetic diversity from exotic germplasm into breeding populations with unprecedented precision, accuracy, and speed. We conclude that the greatest challenge to developing crop varieties to win the race between climate change and food security might be our innovativeness in defining and boldness to breed for the traits of tomorrow

Market‐led options to scale up legume seeds in developing countries: Experiences from the Tropical Legumes Project

There are several hurdles to ensure sustainable seed production and consistent flow of improved legume varieties in sub‐Saharan Africa (SSA) and South Asia (SA). The unreliable demand, autogamous nature of most of the grain legumes, and slow variety replacement rate by smallholder farmers do not provide strong incentive for private seed companies to invest in legume seed business. Unless a well thought‐out and comprehensive approach to legume seed delivery is developed, current seed shortages will continue, eroding emerging market opportunities. The experiences reported here are collated through a 10‐year partnership project, the Tropical Legumes in SSA and SA. It fostered innovative public–private partnerships in joint testing of innovative market‐led seed systems, skills and knowledge enhancement, de‐risking private sector initiatives that introduced in new approaches and previously overlooked entities in technology delivery. As new public and private seed companies, individual seed entrepreneurs and farmer organizations emerged, the existing ones enhanced their capacities. This resulted in significant rise in production, availability and accessibility of various seed grades of newly improved and farmer demanded legume varieties in the target countries

Mapping the medical outcomes study HIV health survey (MOS-HIV) to the EuroQoL 5 Dimension (EQ-5D-3L) utility index

10.1186/s12955-019-1135-8Health and Quality of Life Outcomes1718

Inheritance of resistance to common bacterial blight in four selected common bean (\u3ci\u3ePhaseolus vulgaris\u3c/i\u3e L.) genotypes

Common bacterial blight (CBB) is the most serious bacterial disease of common bean in Uganda. It causes severe yield losses of up to 62%. Genetic resistance is the most effective option for controlling CBB in smallholder common bean production systems. This study was carried out to determine the inheritance pattern of CBB resistance in leaf and pod of four new resistance sources. The four resistant and four susceptible genotypes were crossed in a half-diallel mating design. F1 individuals were advanced to F2 and evaluated with the parents, in a randomized complete block design replicated twice. Combining ability analysis was performed according to Griffing\u27s (1956) method IV and model 1 using Genstat 12th. General combining ability effects were significant whereas specific combining ability was not suggesting that resistance to CBB in leaf and pod was primarily controlled by additive genes effects. The estimated narrow sense coefficient of genetic determination was moderately high (0.65) for the resistance in leaf and high (0.83) for resistance in pod suggesting that early-generation selection would be effective. Baker’s ratio estimates were relatively high for resistance in leaf (0.79) and pod (0.9) suggesting that hybrids’ performance can be predicted based on the parents’ general combining ability (GCA) effects