56 research outputs found
Integrating laboratory and field data to quantify the immersion freezing ice nucleation activity of mineral dust particles
Data from both laboratory studies and atmospheric measurements are used to
develop an empirical parameterization for the immersion freezing activity of
natural mineral dust particles. Measurements made with the Colorado State
University (CSU) continuous flow diffusion chamber (CFDC) when processing
mineral dust aerosols at a nominal 105% relative humidity with respect
to water (RHw) are taken as a measure of the immersion freezing
nucleation activity of particles. Ice active frozen fractions vs. temperature
for dusts representative of Saharan and Asian desert sources were consistent
with similar measurements in atmospheric dust plumes for a limited set of
comparisons available. The parameterization developed follows the form of one
suggested previously for atmospheric particles of non-specific composition in
quantifying ice nucleating particle concentrations as functions of
temperature and the total number concentration of particles larger than
0.5 μm diameter. Such an approach does not explicitly account for
surface area and time dependencies for ice nucleation, but sufficiently
encapsulates the activation properties for potential use in regional and
global modeling simulations, and possible application in developing remote
sensing retrievals for ice nucleating particles. A calibration factor is
introduced to account for the apparent underestimate (by approximately 3, on
average) of the immersion freezing fraction of mineral dust particles for CSU
CFDC data processed at an RHw of 105% vs. maximum fractions
active at higher RHw. Instrumental factors that affect activation
behavior vs. RHw in CFDC instruments remain to be fully explored
in future studies. Nevertheless, the use of this calibration factor is
supported by comparison to ice activation data obtained for the same aerosols
from Aerosol Interactions and Dynamics of the Atmosphere (AIDA) expansion
chamber cloud parcel experiments. Further comparison of the new
parameterization, including calibration correction, to predictions of the
immersion freezing surface active site density parameterization for mineral
dust particles, developed separately from AIDA experimental data alone, shows
excellent agreement for data collected in a descent through a Saharan aerosol
layer. These studies support the utility of laboratory measurements to obtain
atmospherically relevant data on the ice nucleation properties of dust and
other particle types, and suggest the suitability of considering all mineral
dust as a single type of ice nucleating particle as a useful first-order
approximation in numerical modeling investigations
The Effects of Warming-Shifted Plant Phenology on Ecosystem Carbon Exchange Are Regulated by Precipitation in a Semi-Arid Grassland
BACKGROUND: The longer growing season under climate warming has served as a crucial mechanism for the enhancement of terrestrial carbon (C) sink over the past decades. A better understanding of this mechanism is critical for projection of changes in C cycling of terrestrial ecosystems. METHODOLOGY/PRINCIPAL FINDINGS: A 4-year field experiment with day and night warming was conducted to examine the responses of plant phenology and their influences on plant coverage and ecosystem C cycling in a temperate steppe in northern China. Greater phenological responses were observed under night than day warming. Both day and night warming prolonged the growing season by advancing phenology of early-blooming species but without changing that of late-blooming species. However, no warming response of vegetation coverage was found for any of the eight species. The variances in species-level coverage and ecosystem C fluxes under different treatments were positively dependent upon the accumulated precipitation within phenological duration but not the length of phenological duration. CONCLUSIONS/SIGNIFICANCE: These plants' phenology is more sensitive to night than day warming, and the warming effects on ecosystem C exchange via shifting plant phenology could be mediated by precipitation patterns in semi-arid grasslands
STAT3 Regulates Monocyte TNF-Alpha Production in Systemic Inflammation Caused by Cardiac Surgery with Cardiopulmonary Bypass
BACKGROUND: Cardiopulmonary bypass (CPB) surgery initiates a controlled systemic inflammatory response characterized by a cytokine storm, monocytosis and transient monocyte activation. However, the responsiveness of monocytes to Toll-like receptor (TLR)-mediated activation decreases throughout the postoperative course. The purpose of this study was to identify the major signaling pathway involved in plasma-mediated inhibition of LPS-induced tumor necrosis factor (TNF)-α production by monocytes. METHODOLOGY/PRINCIPAL FINDINGS: Pediatric patients that underwent CPB-assisted surgical correction of simple congenital heart defects were enrolled (n = 38). Peripheral blood mononuclear cells (PBMC) and plasma samples were isolated at consecutive time points. Patient plasma samples were added back to monocytes obtained pre-operatively for ex vivo LPS stimulations and TNF-α and IL-6 production was measured by flow cytometry. LPS-induced p38 mitogen-activated protein kinase (MAPK) and nuclear factor (NF)-κB activation by patient plasma was assessed by Western blotting. A cell-permeable peptide inhibitor was used to block STAT3 signaling. We found that plasma samples obtained 4 h after surgery, regardless of pre-operative dexamethasone treatment, potently inhibited LPS-induced TNF-α but not IL-6 synthesis by monocytes. This was not associated with attenuation of p38 MAPK activation or IκB-α degradation. However, abrogation of the IL-10/STAT3 pathway restored LPS-induced TNF-α production in the presence of suppressive patient plasma. CONCLUSIONS/SIGNIFICANCE: Our findings suggest that STAT3 signaling plays a crucial role in the downregulation of TNF-α synthesis by human monocytes in the course of systemic inflammation in vivo. Thus, STAT3 might be a potential molecular target for pharmacological intervention in clinical syndromes characterized by systemic inflammation
The study of atmospheric ice-nucleating particles via microfluidically generated droplets
Ice-nucleating particles (INPs) play a significant role in the climate and hydrological cycle by triggering ice formation in supercooled clouds, thereby causing precipitation and affecting cloud lifetimes and their radiative properties. However, despite their importance, INP often comprise only 1 in 10³–10⁶ ambient particles, making it difficult to ascertain and predict their type, source, and concentration. The typical techniques for quantifying INP concentrations tend to be highly labour-intensive, suffer from poor time resolution, or are limited in sensitivity to low concentrations. Here, we present the application of microfluidic devices to the study of atmospheric INPs via the simple and rapid production of monodisperse droplets and their subsequent freezing on a cold stage. This device offers the potential for the testing of INP concentrations in aqueous samples with high sensitivity and high counting statistics. Various INPs were tested for validation of the platform, including mineral dust and biological species, with results compared to literature values. We also describe a methodology for sampling atmospheric aerosol in a manner that minimises sampling biases and which is compatible with the microfluidic device. We present results for INP concentrations in air sampled during two field campaigns: (1) from a rural location in the UK and (2) during the UK’s annual Bonfire Night festival. These initial results will provide a route for deployment of the microfluidic platform for the study and quantification of INPs in upcoming field campaigns around the globe, while providing a benchmark for future lab-on-a-chip-based INP studies
Social entrepreneurship orientation: Drivers of success for start-ups and established industrial firms
Academic research into social entrepreneurship orientation (SEO) remains nascent, despite social entrepreneurship receiving increasing attention in both research and practice. This study of 308 industrial industry firms from Austria and Northern Macedonia contributes to closing these research gaps by investigating how SEO influences social entrepreneurial performance taking into account the performance consequences of heterogeneity in firms' characteristics demonstrated by start-ups and established firms using multi-group structural equation modeling and fsQCA as methods of comparison. Combining person-centered and variable-centered methods, this study contributes by exploring how SEO influences social entrepreneurial performance and considering firms' characteristics for both start-ups and established industrial firms. The study highlights key differences for start-ups and established firms. The development phase of a firm, thus, plays a key role when examining SEO dimensions. This study informs practices of individuals seeking to undertake a business start-up or owner/managers involved in the operation and management of established firms in a social entrepreneurship context. The findings will be of interest to the enterprise support community in tailoring funding and training support for social enterprises for both start-ups and established firms
Radiation resistance of lactobacilli isolated from radurized meat relative to growth and environment
Integrating laboratory and field data to quantify the immersion freezing ice nucleation activity of mineral dust particles
Data from both laboratory studies and atmospheric measurements are used to
develop an empirical parameterization for the immersion freezing activity of
natural mineral dust particles. Measurements made with the Colorado State
University (CSU) continuous flow diffusion chamber (CFDC) when processing
mineral dust aerosols at a nominal 105% relative humidity with respect
to water (RHw) are taken as a measure of the immersion freezing
nucleation activity of particles. Ice active frozen fractions vs. temperature
for dusts representative of Saharan and Asian desert sources were consistent
with similar measurements in atmospheric dust plumes for a limited set of
comparisons available. The parameterization developed follows the form of one
suggested previously for atmospheric particles of non-specific composition in
quantifying ice nucleating particle concentrations as functions of
temperature and the total number concentration of particles larger than
0.5 μm diameter. Such an approach does not explicitly account for
surface area and time dependencies for ice nucleation, but sufficiently
encapsulates the activation properties for potential use in regional and
global modeling simulations, and possible application in developing remote
sensing retrievals for ice nucleating particles. A calibration factor is
introduced to account for the apparent underestimate (by approximately 3, on
average) of the immersion freezing fraction of mineral dust particles for CSU
CFDC data processed at an RHw of 105% vs. maximum fractions
active at higher RHw. Instrumental factors that affect activation
behavior vs. RHw in CFDC instruments remain to be fully explored
in future studies. Nevertheless, the use of this calibration factor is
supported by comparison to ice activation data obtained for the same aerosols
from Aerosol Interactions and Dynamics of the Atmosphere (AIDA) expansion
chamber cloud parcel experiments. Further comparison of the new
parameterization, including calibration correction, to predictions of the
immersion freezing surface active site density parameterization for mineral
dust particles, developed separately from AIDA experimental data alone, shows
excellent agreement for data collected in a descent through a Saharan aerosol
layer. These studies support the utility of laboratory measurements to obtain
atmospherically relevant data on the ice nucleation properties of dust and
other particle types, and suggest the suitability of considering all mineral
dust as a single type of ice nucleating particle as a useful first-order
approximation in numerical modeling investigations
Identification of Microbial Isolates from Vacuum-Packaged Ground Pork Irradiated at 1 kGy
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