13,204 research outputs found
Compressibility effects on fluid entrainment by turbulent mixing layers
Compressibility effects on fluid entrainment by supersonic turbulent mixing layer
Structural organization of gap junction channels
AbstractGap junctions were initially described morphologically, and identified as semi-crystalline arrays of channels linking two cells. This suggested that they may represent an amenable target for electron and X-ray crystallographic studies in much the same way that bacteriorhodopsin has. Over 30 years later, however, an atomic resolution structural solution of these unique intercellular pores is still lacking due to many challenges faced in obtaining high expression levels and purification of these structures. A variety of microscopic techniques, as well as NMR structure determination of fragments of the protein, have now provided clearer and correlated views of how these structures are assembled and function as intercellular conduits. As a complement to these structural approaches, a variety of mutagenic studies linking structure and function have now allowed molecular details to be superimposed on these lower resolution structures, so that a clearer image of pore architecture and its modes of regulation are beginning to emerge
The Mr 28,000 gap junction proteins from rat heart and liver are different but related
The sequence of the amino-terminal 32 residues of the rat heart Mr 28,000 gap junction protein presented here allows, for the first time, a sequence comparison of gap junctional proteins from different tissues (heart and liver). Comparison of the rat heart gap junction protein sequence and that available from rat liver reveals 43% sequence identity and conservative changes at an additional 25% of the positions. Both proteins exhibit a hydrophobic domain which could represent a transmembrane span of the junction. This result unequivocally demonstrates the existence of at least two forms of the gap junction protein. As yet, no homology is evident between the gap junctional proteins of either heart or liver and main intrinsic protein from rat eye lens
Investigating household recycling behaviour through the interactions between personal and situational factors
In recent years household recycling behaviour (HRB) has become a focal point in social science research to understand the concept of household waste recycling management. Household recycling systems involve two main actors: households and municipalities. This paper reports on an empirical study of the interaction between HRB and household waste recycling systems provided by municipalities. A convenience sample of 412 households was selected to complete a survey on recycling initiatives with personal and situational factors and also their interaction. Results showed that personal factors have a significant relation with situational factors (availability, accessibility, awareness and convenience) (p < 0.01) and vice versa; with a positive correlation (r (412) = +0.41). In addition, personal factors correlated positively to availability, accessibility, awareness and convenience at a p-value below 0.01. Furthermore, situational factors interact with demographical factors such that personal factors may be predicted (overall HRB). This study uses both an interdisciplinary and multi-methods approach to answer its research questions and is also accessible to both practitioner and academic domains. © 2014 WIT Press
INTERACTION OF FLUORIDE COMPLEXES DERIVED FROM GLASS-IONOMER CEMENTS WITH HYDROXYAPATITE
A study has been undertaken of the interaction of complexed fluoride extracted from glass-ionomer dental cements with synthetic hydroxyapatite powder. Extracts were prepared from two commercial glass-ionomers (Fuji IX and ChemFlex) under both neutral and acidic conditions. They were analysed by ICP-OES and by fluoride-ion selective electrode with and without added TISAB to decomplex the fluoride. The pH of the acid extracts was 4, conditions under which fluoride complexes with protons as HF or HF2-, it also complexes with aluminium, which was found to be present in higher amounts in the acid extracts. Fluoride was found to be almost completely complexed in acid extracts, but not in neutral extracts, which contained free fluoride ions. Exposure of these extracts to synthetic hydroxyapatite powder showed that fluoride was taken up rapidly (within 5 minutes), whether or not it was complexed. SEM (EDAX) study of recovered hydroxyapatite showed only minute traces of aluminium taken up under all conditions. This showed that aluminium interacts hardly at all with hydroxyapatite, and hence is probably not involved in the remineralisation process
Titan's atmosphere as observed by Cassini/VIMS solar occultations: CH, CO and evidence for CH absorption
We present an analysis of the VIMS solar occultations dataset, which allows
us to extract vertically resolved information on the characteristics of Titan's
atmosphere between 100-700 km with a characteristic vertical resolution of 10
km. After a series of data treatment procedures, 4 occultations out of 10 are
retained. This sample covers different seasons and latitudes of Titan. The
transmittances show clearly the evolution of the haze and detect the detached
layer at 310 km in Sept. 2011 at mid-northern latitudes. Through the inversion
of the transmission spectra with a line-by-line radiative transfer code we
retrieve the vertical distribution of CH and CO mixing ratio. The two
methane bands at 1.4 and 1.7 {\mu}m are always in good agreement and yield an
average stratospheric abundance of %. This is significantly less
than the value of 1.48% obtained by the GCMS/Huygens instrument. The analysis
of the residual spectra after the inversion shows that there are additional
absorptions which affect a great part of the VIMS wavelength range. We
attribute many of these additional bands to gaseous ethane, whose near-infrared
spectrum is not well modeled yet. Ethane contributes significantly to the
strong absorption between 3.2-3.5 {\mu}m that was previously attributed only to
C-H stretching bands from aerosols. Ethane bands may affect the surface windows
too, especially at 2.7 {\mu}m. Other residual bands are generated by stretching
modes of C-H, C-C and C-N bonds. In addition to the C-H stretch from aliphatic
hydrocarbons at 3.4 {\mu}m, we detect a strong and narrow absorption at 3.28
{\mu}m which we tentatively attribute to the presence of PAHs in the
stratosphere. C-C and C-N stretching bands are possibly present between 4.3-4.5
{\mu}m. Finally, we obtain the CO mixing ratio between 70-170 km. The average
result of ppm is in good agreement with previous studies.Comment: 51 pages, 28 figure
Critical behavior of repulsive linear -mers on triangular lattices
Monte Carlo (MC) simulations and finite-size scaling analysis have been
carried out to study the critical behavior in a submonolayer two-dimensional
gas of repulsive linear -mers on a triangular lattice at coverage
. A low-temperature ordered phase, characterized by a repetition of
alternating files of adsorbed -mers separated by adjacent empty sites,
is separated from the disordered state by a order-disorder phase transition
occurring at a finite critical temperature, . The MC technique was
combined with the recently reported Free Energy Minimization Criterion Approach
(FEMCA), [F. Rom\'a et al., Phys. Rev. B, 68, 205407, (2003)], to predict the
dependence of the critical temperature of the order-disorder transformation.
The dependence on of the transition temperature, , observed in MC
is in qualitative agreement with FEMCA. In addition, an accurate determination
of the critical exponents has been obtained for adsorbate sizes ranging between
and . For , the results reveal that the system does not belong
to the universality class of the two-dimensional Potts model with (,
monomers). Based on symmetry concepts, we suggested that the behavior observed
for and 3 could be generalized to include larger particle sizes ().Comment: 17 pages, 13 figure
Modified Zakharov equations for plasmas with a quantum correction
Quantum Zakharov equations are obtained to describe the nonlinear interaction
between quantum Langmuir waves and quantum ion-acoustic waves. These quantum
Zakharov equations are applied to two model cases, namely the four-wave
interaction and the decay instability. In the case of the four-wave
instability, sufficiently large quantum effects tend to suppress the
instability. For the decay instability, the quantum Zakharov equations lead to
results similar to those of the classical decay instability except for quantum
correction terms in the dispersion relations. Some considerations regarding the
nonlinear aspects of the quantum Zakharov equations are also offered.Comment: 4 figures. Accepted for publication in Physics of Plasmas (2004
Automatic Spectroscopic Data Categorization by Clustering Analysis (ASCLAN): A Data-Driven Approach for Distinguishing Discriminatory Metabolites for Phenotypic Subclasses
We propose a novel data-driven approach aiming to reliably distinguish discriminatory metabolites from nondiscriminatory metabolites for a given spectroscopic data set containing two biological phenotypic subclasses. The automatic spectroscopic data categorization by clustering analysis (ASCLAN) algorithm aims to categorize spectral variables within a data set into three clusters corresponding to noise, nondiscriminatory and discriminatory metabolites regions. This is achieved by clustering each spectral variable based on the r(2) value representing the loading weight of each spectral variable as extracted from a orthogonal partial least-squares discriminant (OPLS-DA) model of the data set. The variables are ranked according to r(2) values and a series of principal component analysis (PCA) models are then built for subsets of these spectral data corresponding to ranges of r(2) values. The Q(2)X value for each PCA model is extracted. K-means clustering is then applied to the Q(2)X values to generate two clusters based on minimum Euclidean distance criterion. The cluster consisting of lower Q(2)X values is deemed devoid of metabolic information (noise), while the cluster consists of higher Q(2)X values is then further subclustered into two groups based on the r(2) values. We considered the cluster with high Q(2)X but low r(2) values as nondiscriminatory, while the cluster with high Q(2)X and r(2) values as discriminatory variables. The boundaries between these three clusters of spectral variables, on the basis of the r(2) values were considered as the cut off values for defining the noise, nondiscriminatory and discriminatory variables. We evaluated the ASCLAN algorithm using six simulated (1)H NMR spectroscopic data sets representing small, medium and large data sets (N = 50, 500, and 1000 samples per group, respectively), each with a reduced and full resolution set of variables (0.005 and 0.0005 ppm, respectively). ASCLAN correctly identified all discriminatory metabolites and showed zero false positive (100% specificity and positive predictive value) irrespective of the spectral resolution or the sample size in all six simulated data sets. This error rate was found to be superior to existing methods for ascertaining feature significance: univariate t test by Bonferroni correction (up to 10% false positive rate), Benjamini-Hochberg correction (up to 35% false positive rate) and metabolome wide significance level (MWSL, up to 0.4% false positive rate), as well as by various OPLS-DA parameters: variable importance to projection, (up to 15% false positive rate), loading coefficients (up to 35% false positive rate), and regression coefficients (up to 39% false positive rate). The application of ASCLAN was further exemplified using a widely investigated renal toxin, mercury II chloride (HgCl2) in rat model. ASCLAN successfully identified many of the known metabolites related to renal toxicity such as increased excretion of urinary creatinine, and different amino acids. The ASCLAN algorithm provides a framework for reliably differentiating discriminatory metabolites from nondiscriminatory metabolites in a biological data set without the need to set an arbitrary cut off value as applied to some of the conventional methods. This offers significant advantages over existing methods and the possibility for automation of high-throughput screening in "omics" data
Surface phase transitions in one-dimensional channels arranged in a triangular cross-sectional structure: Theory and Monte Carlo simulations
Monte Carlo simulations and finite-size scaling analysis have been carried
out to study the critical behavior in a submonolayer lattice-gas of interacting
monomers adsorbed on one-dimensional channels arranged in a triangular
cross-sectional structure. The model mimics a nanoporous environment, where
each nanotube or unit cell is represented by a one-dimensional array. Two kinds
of lateral interaction energies have been considered: , interaction
energy between nearest-neighbor particles adsorbed along a single channel and
, interaction energy between particles adsorbed across
nearest-neighbor channels. For and , successive planes are
uncorrelated, the system is equivalent to the triangular lattice and the
well-known
ordered phase is found at low temperatures and a coverage, , of 1/3
. In the more general case ( and ), a
competition between interactions along a single channel and a transverse
coupling between sites in neighboring channels allows to evolve to a
three-dimensional adsorbed layer. Consequently, the and structures "propagate" along the
channels and new ordered phases appear in the adlayer. The Monte Carlo
technique was combined with the recently reported Free Energy Minimization
Criterion Approach (FEMCA), to predict the critical temperatures of the
order-disorder transformation. The excellent qualitative agreement between
simulated data and FEMCA results allow us to interpret the physical meaning of
the mechanisms underlying the observed transitions.Comment: 24 pages, 6 figure
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