Biogenesis of mitochondrial c-type cytochromes

Cytochromesc andc 1 are essential components of the mitochondrial respiratory chain. In both cytochromes the heme group is covalently linked to the polypeptide chain via thioether bridges. The location of the two cytochromes is in the intermembrane space; cytochromec is loosely attached to the surface of the inner mitochondrial membrane, whereas cytochromec 1 is firmly anchored to the inner membrane. Both cytochromec andc 1 are encoded by nuclear genes, translated on cytoplasmic ribosomes, and are transported into the mitochondria where they become covalently modified and assembled. Despite the many similarities, the import pathways of cytochromec andc 1 are drastically different. Cytochromec 1 is made as a precursor with a complex bipartite presequence. In a first step the precursor is directed across outer and inner membranes to the matrix compartment of the mitochondria where cleavage of the first part of the presequence takes place. In a following step the intermediate-size form is redirected across the inner membrane; heme addition then occurs on the surface of the inner membrane followed by the second processing reaction. The import pathway of cytochromec is exceptional in practically all aspects, in comparison with the general import pathway into mitochondria. Cytochromec is synthesized as apocytochromec without any additional sequence. It is translocated selectively across the outer membrane. Addition of the heme group, catalyzed by cytochromec heme lyase, is a requirement for transport. In summary, cytochromec 1 import appears to follow a conservative pathway reflecting features of cytochromec 1 sorting in prokaryotic cells. In contrast, cytochromec has invented a rather unique pathway which is essentially non-conservative

Efficient Learning of a One-dimensional Density Functional Theory

Density functional theory underlies the most successful and widely used numerical methods for electronic structure prediction of solids. However, it has the fundamental shortcoming that the universal density functional is unknown. In addition, the computational result---energy and charge density distribution of the ground state---is useful for electronic properties of solids mostly when reduced to a band structure interpretation based on the Kohn-Sham approach. Here, we demonstrate how machine learning algorithms can help to free density functional theory from these limitations. We study a theory of spinless fermions on a one-dimensional lattice. The density functional is implicitly represented by a neural network, which predicts, besides the ground-state energy and density distribution, density-density correlation functions. At no point do we require a band structure interpretation. The training data, obtained via exact diagonalization, feeds into a learning scheme inspired by active learning, which minimizes the computational costs for data generation. We show that the network results are of high quantitative accuracy and, despite learning on random potentials, capture both symmetry-breaking and topological phase transitions correctly.Comment: 5 pages, 3 figures; 4+ pages appendi

Space Station Spartan study

The required extension, enhancement, and upgrading of the present Spartan concept are described to conduct operations from the space station using the station's unique facilities and operational features. The space station Spartan (3S), the free flyer will be deployed from and returned to the space station and will conduct scientific missions of much longer duration than possible with the current Spartan. The potential benefits of a space station Spartan are enumerated. The objectives of the study are: (1) to develop a credible concept for a space station Spartan; and (2) to determine the associated requirements and interfaces with the space station to help ensure that the 3S can be properly accommodated

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This is an original manuscript dedicated to the owner of this collection.https://scholarexchange.furman.edu/periphery-neupert/1001/thumbnail.jp

The nature of the fluorescent iron line in V 1486 Ori

The fluorescent 6.4 keV iron line provides information on cool material in the vicinity of hard X-ray sources as well as on the characteristics of the X-ray sources themselves. First discovered in the X-ray spectra of the flaring Sun, X-ray binaries and active galactic nuclei (AGN), the fluorescent line was also observed in a number of stellar X-ray sources. The young stellar object (YSO) V1486 Ori was observed in the framework of the Chandra Ultra Deep Project (COUP) as the source COUP 331. We investigate its spectrum, with emphasis on the strength and time variability of the fluorescent iron K-alpha line, derive and analyze the light curve of COUP 331 and proceed with a time-resolved spectral analysis of the observation. The light curve of V 1486 Ori shows two major flares, the first one lasting for (approx) 20 ks with a peak X-ray luminosity of 2.6*10^{32} erg/s (dereddened in the 1-10 keV band) and the second one -- only partially observed -- for >60 ks with an average X-ray luminosity of 2.4*10^{31} erg/s (dereddened). The spectrum of the first flare is very well described by an absorbed thermal model at high temperature, with a pronounced 6.7 keV iron line complex, but without any fluorescent K-alpha line. The X-ray spectrum of the second flare is characterized by even higher temperatures (>= 10 keV) without any detectable 6.7 keV Fe XXV feature, but with a very strong fluorescent iron K-alpha line appearing predominantly in the 20 ks rise phase of the flare. Preliminary model calculations indicate that photoionization is unlikely to account for the entire fluorescent emission during the rise phase.Comment: 4 pages, letter, accepted for publication in A&

Carbon coating of the SPS dipole chambers

The Electron Multipacting (EM) phenomenon is a limiting factor for the achievement of high luminosity in accelerators for positively charged particles and for the performance of RF devices. At CERN, the Super Proton Synchrotron (SPS) must be upgraded in order to feed the Large Hadron Collider (LHC) with 25 ns bunch spaced beams. At such small bunch spacing, EM may limit the performance of the SPS and consequently that of the LHC. To mitigate this phenomenon CERN is developing a carbon thin film coating with low Secondary Electron Yield (SEY) to coat the internal walls of the SPS dipoles beam pipes. This paper presents the progresses in the coating technology, the performance of the carbon coatings and the strategy for a large scale production.Comment: 7 pages, contribution to the Joint INFN-CERN-EuCARD-AccNet Workshop on Electron-Cloud Effects: ECLOUD'12; 5-9 Jun 2012, La Biodola, Isola d'Elba, Italy; CERN Yellow Report CERN-2013-002, pp.141-14

Puromycin Sensitivity of Ribosomal Label after Incorporation of 14C-Labelled Amino Acids into Isolated Mitochondria from Neurospora crassa

Radioactive amino acids were incorporated into isolated mitochondria from Neurospora crassa. Then the mitochondrial ribosomes were isolated and submitted to density gradient centrifugation. A preferential labelling of polysomes was observed. However, when the mitochondrial suspension was treated with puromycin after amino acid incorporation, no radioactivity could be detected in either the monosomes or the polysomes. The conclusion is drawn that isolated mitochondria under these conditions do not incorporate significant amounts of amino acids into proteins of their ribosomes