Long-Term Changes of Central Ocular Motor Signs in Patients with Vestibular Migraine

Background: A high percentage of patients with vestibular migraine (VM)were reported to have central ocular motor dysfunctions (COMD) in thesymptom-free interval. Since VM is a chronic disorder, it is of interestif COMD worsen over time. Methods: Thirty VM patients and 11 controlpatients participated in an 8-year-long observational study withfollow-up. All patients underwent standardized neuro-ophthalmologicaland neuro-otological examinations at the initial presentation and atfollow-up. The primary outcome measure was change in COMD at follow-up.Results: In general, COMD in the symptom-free interval were subtle,consisting of a saccadic horizontal and/or vertical pursuit

Correlation of crystal violet biofilm test results of Staphylococcus aureus clinical isolates with Raman spectroscopic read-out

Biofilm-related infections occur quite frequently in hospital settings and require rapid diagnostic identification as they are recalcitrant to antibiotic therapy and make special treatment necessary. One of the standard microbiological in vitro tests is the crystal violet test. It indirectly determines the amount of biofilm by measuring the optical density (OD) of the crystal violet-stained biofilm matrix and cells. However, this test is quite time-consuming, as it requires bacterial cultivation up to several days. In this study, we correlate fast Raman spectroscopic read-out of clinical Staphylococcus aureus isolates from 47 patients with different disease background with their biofilm-forming characteristics. Included were low (OD  20) biofilm performers as determined by the crystal violet test. Raman spectroscopic analysis of the bacteria revealed most spectral differences between high and low biofilm performers in the fingerprint region between 750 and 1150 cm−1. Using partial least square regression (PLSR) analysis on the Raman spectra involving the three categories of biofilm formation, it was possible to obtain a slight linear correlation of the Raman spectra with the biofilm OD values. The PLSR loading coefficient highlighted spectral differences between high and low biofilm performers for Raman bands that represent nucleic acids, carbohydrates, and proteins. Our results point to a possible application of Raman spectroscopy as a fast prediction tool for biofilm formation of bacterial strains directly after isolation from the infected patient. This could help clinicians make timely and adapted therapeutic decision in future

Casting new light on the chronology of the loess/paleosol sequences in Lower Austria

Der vorliegende Artikel gibt einen Überblick über neu datierte Abschnitte in bekannten Löss/Paläoboden-Sequenzen Niederösterreichs. Die Ergebnisse der Datierungen im Profil Joching deuten darauf hin, dass es im letzten Hochglazial zur Lösssedimentation kam. Die meisten erfassten Alter sind jedoch älter als das letzte Hochglazial, was auf Erosionsprozesse hindeutet, die zur Abtragung der jüngeren Lösse geführt hat. In dem Abschnitt zwischen ~28 ka and ~35 ka wurden überwiegend Tundragleye gebildet. Eine intensivere interstadiale Bodenbildung ist nicht nachzuweisen. Dieses Ergebnis kann auch für die stratigraphische Einstufung von ‚Stillfried B‘ (sensu Fink) von Bedeutung sein. Der folgende chronologische Abschnitt liegt zwischen ~35 ka and ~57 ka in Lösssedimenten mit eingeschalteten Tundragleyen. Auch dieser Abschnitt ist durch Umlagerungsprozesse charakterisiert. Im Zeitraum von ~57 ka bis ~106 ka befindet sich eine markante Zeitlücke, die vermutlich auf langandauernde und intensive Erosionsprozesse im Untersuchungsgebiet zurückzuführen ist. Die älteste Datierung in den Sedimenten des letzten Glazials mit 106 ± 12 ka befindet sich in Paudorf direkt über dem ‚Stillfried A‘- Komplex (Paudorfer Bodenbildung). Direkt unter diesem Pedokomplex, bzw. vergleichbaren Pedokomplexen treten in Lössablagerungen Alter von 124 ± 2 5 ka (Göttweig-Aigen), 159 ± 20 ka (Paudorf 1), and 170 ± 16 ka (Joching) auf. Darüber hinausgehende Alter konnten in Stratzing, Paudorf 2, Göttweig-Furth und Langenlois nachgewiesen werden

Nerve Growth Factor Serum Levels Are Associated With Regional Gray Matter Volume Differences in Schizophrenia Patients

Numerous neuroimaging studies have revealed structural brain abnormalities in schizophrenia patients. There is emerging evidence that dysfunctional nerve growth factor (NGF) signaling may contribute to structural brain alterations found in these patients. In this pilot study, we investigated whether there was a correlation between NGF serum levels and gray matter volume (GMV) in schizophrenia patients. Further, we investigated whether there was an overlap between the correlative findings and cross-sectional GMV differences between schizophrenia patients (n = 18) and healthy controls (n = 19). Serum NGF was significantly correlated to GMV in the left prefrontal lobe, the left midcingulate cortex, and the brainstem in schizophrenia patients. However, we did not find any correlations of NGF serum levels with GMV in healthy controls. Schizophrenia patients showed smaller GMV than healthy controls in brain regions located in the bilateral limbic system, bilateral parietal lobe, bilateral insula, bilateral primary auditory cortex, left frontal lobe, and bilateral occipital regions. In a conjunction analysis, GMV in the left midcingulate cortex (MCC) appears negatively correlated to NGF serum levels in the group of schizophrenia patients and also to be reduced compared to healthy controls. These results suggest an increased vulnerability of schizophrenia patients to changes in NGF levels compared to healthy controls and support a role for NGF signaling in the pathophysiology of schizophrenia. As our pilot study is exploratory in nature, further studies enrolling larger sample sizes will be needed to further corroborate our findings and to investigate the influence of additional covariates

Effectiveness of screening preschool children for amblyopia: a systematic review

<p>Abstract</p> <p>Background</p> <p>Amblyopia and amblyogenic factors like strabismus and refractive errors are the most common vision disorders in children. Although different studies suggest that preschool vision screening is associated with a reduced prevalence rate of amblyopia, the value of these programmes is the subject of a continuing scientific and health policy discussion. Therefore, this systematic review focuses on the question of whether screening for amblyopia in children up to the age of six years leads to better vision outcomes.</p> <p>Methods</p> <p>Ten bibliographic databases were searched for randomised controlled trials, non-randomised controlled trials and cohort studies with no limitations to a specific year of publication and language. The searches were supplemented by handsearching the bibliographies of included studies and reviews to identify articles not captured through our main search strategy.</p> <p>Results</p> <p>Five studies met the inclusion criteria. Of these, three studies suggested that screening is associated with an absolute reduction in the prevalence of amblyopia between 0.9% and 1.6% (relative reduction: between 45% and 62%). However, the studies showed weaknesses, limiting the validity and reliability of their findings. The main limitation was that studies with significant results considered only a proportion of the originally recruited children in their analysis. On the other hand, retrospective sample size calculation indicated that the power based on the cohort size was not sufficient to detect small changes between the groups. Outcome parameters such as quality of life or adverse effects of screening have not been adequately investigated in the literature currently available.</p> <p>Conclusion</p> <p>Population based preschool vision screening programmes cannot be sufficiently assessed by the literature currently available. However, it is most likely that the present systematic review contains the most detailed description of the main limitations in current available literature evaluating these programmes. Therefore, future research work should be guided by the findings of this publication.</p

A Modular BAM Complex in the Outer Membrane of the α-Proteobacterium Caulobacter crescentus

Mitochondria are organelles derived from an intracellular α-proteobacterium. The biogenesis of mitochondria relies on the assembly of β-barrel proteins into the mitochondrial outer membrane, a process inherited from the bacterial ancestor. Caulobacter crescentus is an α-proteobacterium, and the BAM (β-barrel assembly machinery) complex was purified and characterized from this model organism. Like the mitochondrial sorting and assembly machinery complex, we find the BAM complex to be modular in nature. A ∼150 kDa core BAM complex containing BamA, BamB, BamD, and BamE associates with additional modules in the outer membrane. One of these modules, Pal, is a lipoprotein that provides a means for anchorage to the peptidoglycan layer of the cell wall. We suggest the modular design of the BAM complex facilitates access to substrates from the protein translocase in the inner membrane