TREATMENT WITH APHERESIS

No abstrac

FLOWCYTOMETRIC STUDIES OF CELL IMMUNITY IN PATIENTS WITH URAEMIA ON PERIODIC HAEMODIALYSIS AND AFTER RENAL TRANSPLANTATION

The course of a series of kidney diseases is characterized by impaired immunity additionally altered during haemodialysis and after renal transplantation. The study covered 50 patients divided into three groups: 16 patients with chronic renal failure (CRF), 23 ones on periodic haemodialysis (PHD), and 11 after renal transplantation. Flowcytometric immunophenotyping by means of FACS-analysis using monoclonal antibodies was carried out. The investigation aimed at revealing the changes in the cell immunity, activation of surface markers and adhesion molecules in these patients. Statistically significant changes in the expression of 1С AM-1 adhesion molecules were established as followed: CD54+/ CD4+ = 24,8 ± 11,6 (in CRF) against 14,9 ±8,1 (in PHD) atp < 0,05 and CD54+/CD8+ = 17 ± 6,9 (in CRF) against 10,3 ± 4,8 (in PHD) at p < 0,05. A conclusion was drawn that immune response damage in PHD patients resulted mainly from the lowered expression of the adhesion molecules of the ICAM-1 immunoglobulin gene superfamily

USE OF INTENSIVE METHODS FOR TREATMENT OF RENAL DISEASES

No abstrac

OPTIMAL WEIGHT AND DIALYSIS DOSE IN PATIENTS ON PERIODIC HEMODIALYSIS

Small patients tend to be better dialyzed than large ones. We analyzed the delivered dose of dialysis in two groups of patients - group A, body weight over 50 kg (n-39, m:f=28:l; 57,4 8,3 kg) and group B, body weight under 50 kg (n=15, m:f=6:9; 45,4 3,4 kg). We calculated KT/V and Time Average Concentration of urea (TAC) using two-pool method for urea kinetic modeling. The patients from group Ð’ had a higher KT/V urea =1,35 0,25 (p < 0,05) and lower TAC =13,7 2,72 (p < 0,05) with a shorter dialysis time. Mean serum protein and albumin levels for a year did not differ

Two-dimensional electronic spectroscopy as a tool for tracking molecular conformations in DNA/RNA aggregates

A computational strategy to simulate two-dimensional electronic spectra (2DES) is introduced, which allows characterising ground state conformations of flexible nucleobase aggregates that play a crucial role in nucleic acid photochemistry

Environment-Driven Coherent Population Transfer Governs the Ultrafast Photophysics of Tryptophan

By combining UV transient absorption spectroscopy with sub-30-fs temporal resolution and CASPT2/MM calculations, we present a complete description of the primary photo-induced processes in solvated tryptophan. Our results shed new light on the role of the solvent in the relaxation dynamics of tryptophan. We unveil two consecutive coherent population transfer events involving the lowest two singlet excited states: a sub-50-fs non-adiabatic La--&gt;Lb transfer through a conical intersection and a subsequent 220 fs reverse Lb--&gt;La transfer due to solvent assisted adiabatic stabilization of the La state. Vibrational fingerprints in the transient absorption spectra provide compelling evidence of a vibronic coherence established between the two excited states from the earliest times after photoexcitation and lasting until the back-transfer to La is complete. The demonstration of response to the environment as a driver of coherent population dynamics among the excited states of tryptophan closes the long debate on its solvent-assisted relaxation mechanisms and extends its application as a local probe of protein dynamics to the ultrafast timescales

Post-streptococcal glomerulonephritis is a strong risk factor for chronic kidney disease in later life

Although unusual in western countries and in Australia in general, post-streptococcal glomerulonephritis (PSGN) is still common in Australian Aboriginal children living in remote communities. Here, we evaluated whether episodes of acute PSGN increased the risk for chronic kidney disease in later life in 1519 residents of a remote Aboriginal community (85% of those age eligible), with high rates of renal and cardiovascular disease, who participated in a health screen over a 3-year period. Of these, 200 had had at least one episode of PSGN, with 27 having had multiple episodes, usually in childhood. High levels of albuminuria (albumin/creatinine ratio) with increasing age were confirmed. All PSGN episodes were associated with group A streptococcal skin infections, often related to scabies. In both genders, aged 10-39 years at screening, about one in five had such a history. Among them, PSGN (5 years or more earlier) was significantly associated with higher levels of albuminuria than those without. In women, aged 30-39 years, a history of PSGN was associated with a significantly higher frequency of estimated glomerular filtration rates < 60 ml/min. The adjusted odds ratios for an albumin/creatinine ratio over 34 g/mol (overt albuminuria) in males and females with a history of PSGN were 4.6 and 3.1, respectively, compared with those without a history. Thus, PSGN contributes to the very serious burden of chronic kidney disease in this community. Rigorous strategies to prevent scabies and Group A streptococcal infections will reduce this burden