THE DISEASES AND PESTS CONTROL IN THE FARMING CULTURES, USING BIOPRODUCTS

The making of a performant agriculture is influenced among other things by the applicative working technologies, the phyto-sanitary protection having an important role within these technologies.The current research and studies concerning the methods and equipments of applying of phyto-sanitary treatments, are enrolled in the new trends for applying a long lasting agriculture; the phyto-sanitary protection represents one of the main sources of the environment poluation resources.In the first part of the work is presented a syntesis of the aspects concerning the impact of the phytosanitary treatments used in agriculture upon environment, the methods used these days for diseases and pests control and the future  trends  with referrence to the technologies and products used in  the agricultural ecological practice and at the end are presented products already used or being at the begining using phase

CONSTRUCTION TYPES OF TECHNICAL EQUIPMENT FOR DOSING, WEIGHING, PACKAGING AND PROCEDURES USED IN MILLING UNITS

The paper presents several types of technical equipment for dosing, weighing, packaging and the procedures used in the milling units. The weighing systems take into account several important criteria for their design, namely: how the weight or mass of the load is offset and how the result is transferred

Profunda femoris reconstruction – a solution for inferior limb revascularization

Clinica Chirurgie II, Spitalul Clinic Judeţean de Urgenţă Mureş, Clinica Medicală II, Spitalul Clinic Judeţean de Urgenţă Mureş, Clinica Chirurgie Cardiovasculară II, IBCVT Tg. MureşProfunda femoris rămâne singura sursă vasculară pentru salvarea membrului inferior în cazurile leziunilor avansate ocluzive ale arterei femurale superficiale; există diverse proceduri chirurgicale, descrise sub numele de profundoplastii, preferinţa pentru o metodă sau alta bazându-se pe experienţă, situaţie anatomică intraoperatorie, preferințele chirurgului. În toate cazurile rezultatul reconstrucţiei este în strictă dependenţă de calitatea runoff-ului. Calibrul redus al vasului, absenţa colateralelor şi/sau ocluzia vaselor distale întunecă pronosticul postoperator sau chiar contraindică metoda. Scopul acestei lucrări este de a analiza rezultatele obţinute după profundoplastiile efectuate de autori în serviciul nostru.Profunda femoris remains the single vascular source for limb salvage in cases with superficial femoral artery obstruction; there are a lot of surgical procedures described under the name of profundoplasty, using one or another being a matter of experience, pre- or intraop anatomical findings, surgeons preference. The postop outcome depends on the quality of the run-off-poor collateralization or distal vascular obstructions are the worst predictive factors for surgical failure. The aim of this clinical retrospective study is to analyze the authors experience regarding this issue

Damage control surgery in abdominal traumatology. Three cases report

Clinica Chirurgie II, Spitalul Clinic Judeţean de Urgenţă MureşScopul principal al studiului clinic este de a accentua importanța chirurgiei de control lezional în traumele majore abdominale. Cu toate că nu am realizat un studiu retrospectiv al experienţei noastre în domeniu, am considerat util să prezentăm trei situaţii clinice în care pentru leziuni diferite chirurgia de control lezional a fost urmată de rezultate favorabile, atrăgând atenția asupra indicațiilor, criteriilor clinice, paraclinice și operatorii care determină utilizarea metodei.The main purpose of this clinical study is to emphasize the importance of the damage control surgery in major abdominal trauma. Although we do not made a statistical retrospective analysis of our experience in this issue, we describe here three of our cases where we performed damage control surgery with good postoperative results, drawing attention upon indications, clinical, paraclinical and operative criteria which indicate the method

Apple polyphenols in human and animal health*

Apples contain substantial amounts of polyphenols, and diverse phenolics mainly flavonoids and phenolic acids, have been identified in their flesh and skins. This work aimed to analyze the overall landscape of the research literature published to date on apple phenolic compounds in the context of human and animal health. The Web of Science Core Collection electronic database was queried with (apple* polyphenol*) AND (health* OR illness* OR disease* OR medic* OR pharma*) to identify relevant papers covering these words and their derivatives in the titles, abstracts, and keywords. The resulted 890 papers were bibliometrically analyzed. The VOSviewer software was utilized to produce term maps that illustrate how the frequent phrases fared in terms of publication and citation data. The apple polyphenol papers received global contributions, particularly from China, Italy, the United States, Spain, and Germany. Examples of frequently mentioned chemicals/chemical classes are quercetin, anthocyanin, catechin, epicatechin, and flavonol, while examples of frequently mentioned medical conditions are cardiovascular disease, atherosclerosis, diabetes, Alzheimers disease, and obesity. The potential health benefits of apple polyphenols on humans and animals are diverse and warrant further study.Authors acknowledge the support from The National Centre for Research and Development (NCBR) of Poland (project number POIR.01.01.01-00-0593/18).info:eu-repo/semantics/publishedVersio

Progresses towards safe and efficient gene therapy vectors

The emergence of genetic engineering at the beginning of the 1970′s opened the era of biomedical technologies, which aims to improve human health using genetic manipulation techniques in a clinical context. Gene therapy represents an innovating and appealing strategy for treatment of human diseases, which utilizes vehicles or vectors for delivering therapeutic genes into the patients’ body. However, a few past unsuccessful events that negatively marked the beginning of gene therapy resulted in the need for further studies regarding the design and biology of gene therapy vectors, so that this innovating treatment approach can successfully move from bench to bedside. In this paper, we review the major gene delivery vectors and recent improvements made in their design meant to overcome the issues that commonly arise with the use of gene therapy vectors. At the end of the manuscript, we summarized the main advantages and disadvantages of common gene therapy vectors and we discuss possible future directions for potential therapeutic vectors.This work is part of research grant No. 128/2014; PNII- PT-PCCA-2013-4-2166 “New strategies for improving life quality and survival in cancer patients: molecular and clinical studies of the tumor genome in deuterium-depleted water treatment augmentation - GenCanD”. Dr Calin is The Alan M. Gewirtz Leukemia & Lymphoma Society Scholar. Work in Dr. Calin’s laboratory is supported in part by the NIH/NCI grants 1UH2TR00943-01 and 1 R01 CA182905-01, the UT MD Anderson Cancer Center SPORE in Melanoma grant from NCI (P50 CA093459), Aim at Melanoma Foundation and the Miriam and Jim Mulva research funds, the Brain SPORE (2P50CA127001), the Center for Radiation Oncology Research Project, the Center for Cancer Epigenetics Pilot project, a 2014 Knowledge GAP MDACC grant, a CLL Moonshot pilot project, the UT MD Anderson Cancer Center Duncan Family Institute for Cancer Prevention and Risk Assessment, a SINF grant in colon cancer, the Laura and John Arnold Foundation, the RGK Foundation and the Estate of C. G. Johnson, Jr,.http://www.impactjournals.com/oncotargetam2016Immunolog

MiR-543 regulates the epigenetic landscape of myelofibrosis by targeting TET1 and TET2

Myelofibros is (MF) is a myeloproliferative neoplasm characterized by cytopenia and extramedullary hematopoiesis, resulting in splenomegaly. Multiple pathological mechanisms (e.g., circulating cytokines and genetic alterations, such as JAK(V617F) mutation) have been implicated in the etiology of MF, but the molecular mechanism causing resistance to JAK(V617F) inhibitor therapy remains unknown. Among MF patients who were treated with the JAK inhibitor ruxolitinib, we compared noncoding RNA profiles of ruxolitinib therapy responders versus nonresponders and found miR-S43 was significantly upregulated in non responders. We validated these findings by reverse transcription-quantitative PCR. in this same cohort, in 2 additional independent MF patient cohorts from the United States and Romania, and in a JAK2(V617F) mouse model of MF. Both in vitro and in vivo models were used to determine the underlying molecular mechanism of miR-543 in MF. Here, we demonstrate that miR-543 targets the dioxygenases ten-eleven translocation 1 (TET1) and 2 (TET2) in patients and in vitro, causing increased levels of global 5-methylcytosine, while decreasing the acetylation of histone 3, STAT3, and tumor protein p53. Mechanistically, we found that activation of STAT3 by JAKs epigenetically controls miR-543 expression via binding the promoter region of miR-543. Furthermore, miR-543 upregulation promotes the expression of genes related to drug metabolism, including CYP3A4, which is involved in ruxolitinib metabolism. Our findings suggest miR-543 as a potentially novel biomarker for the prognosis of MF patients with a high risk of treatment resistance and as a potentially new target for the development of new treatment options

Conformation-regulated mechanosensory control via titin domains in cardiac muscle

The giant filamentous protein titin is ideally positioned in the muscle sarcomere to sense mechanical stimuli and transform them into biochemical signals, such as those triggering cardiac hypertrophy. In this review, we ponder the evidence for signaling hotspots along the titin filament involved in mechanosensory control mechanisms. On the way, we distinguish between stress and strain as triggers of mechanical signaling events at the cardiac sarcomere. Whereas the Z-disk and M-band regions of titin may be prominently involved in sensing mechanical stress, signaling hotspots within the elastic I-band titin segment may respond primarily to mechanical strain. Common to both stress and strain sensor elements is their regulation by conformational changes in protein domains