ANALYZING ECOHYDROLOGY OF SUBIRRIGATED MEADOW, DRY VALLEY AND UPLAND DUNE ECOSYSTEMS USING REMOTE SENSING AND IN-SITU ESTIMATIONS IN THE SEMIARID SAND HILLS REGION OF NEBRASKA, USA

Nebraska’s dependence upon the High Plains (Ogallala) Aquifer for agricultural production is vital to the state’s economy, ecology and hydrology. The Sand Hills region (58,000 km2) of Nebraska is a unique system of lakes, (~5%) wetlands, (~10%) subirrigated meadows, (~20%) dry valleys and (~65%) upland sand dune ecosystems. Understanding how each of these land cover types reacts to climate conditions of different water limitations is vital to regional water resource management. This research explores the ecohydrological behavior of different land cover types at the Gudmundsen Sand Hills Research Laboratory (GSRL) near Whitman, Nebraska in the heart of the Sand Hills region of Nebraska by using remote sensing and in-situ estimations of energy partitioning. By employing satellite technology and micrometeorological instrumentation this research establishes a better understanding how energy partitioning, and resulting evapotranspiration (ET), differs between different vegetative communities. We present findings of diurnal and seasonal estimates of energy partitioning as well as daily estimations of ET from both satellite image processing and in-situ observations by Bowen ratio energy balance systems (BREBS). This research also employed different techniques to estimate energy partitioning via remote sensing by adjusting radiation, wind speed, and stability parameters to better represent areas with high topographic relief. The last focal point of this research was to analyze how energy partitioning and ET varied both spatially and temporally under different climate conditions between 2004 (normal year), 2006 (dry year), and 2009 (wet year). Adviser: John D. Lenter

Examination of Surface Temperature Modification by Open-Top Chambers along Moisture and Latitudinal Gradients in Arctic Alaska Using Thermal Infrared Photography

Passive warming manipulation methodologies, such as open-top chambers (OTCs), are a meaningful approach for interpretation of impacts of climate change on the Arctic tundra biome. The magnitude of OTC warming has been studied extensively, revealing an average plot-level warming of air temperature that ranges between 1 and 3 °C as measured by shielded resistive sensors or thermocouples. Studies have also shown that the amount of OTC warming depends in part on location climate, vegetation, and soil properties. While digital infrared thermometers have been employed in a few comparisons, most of the focus of the effectiveness of OTC warming has been on air or soil temperature rather than tissue or surface temperatures, which directly translate to metabolism. Here we used thermal infrared (TIR) photography to quantify tissue and surface temperatures and their spatial variability at a previously unavailable resolution (3–6 mm2). We analyzed plots at three locations that are part of the International Tundra Experiment (ITEX)-Arctic Observing Network (AON-ITEX) network along both moisture and latitudinal gradients spanning from the High Arctic (Barrow, AK, USA) to the Low Arctic (Toolik Lake, AK, USA). Our results show a range of OTC surface warming from 2.65 to 1.27 °C (31%–10%) at our three sites. The magnitude of surface warming detected by TIR imagery in this study was comparable to increases in air temperatures previously reported for these sites. However, the thermal images revealed wide ranges of surface temperatures within the OTCs, with some surfaces well above ambient unevenly distributed within the plots under sunny conditions. We note that analyzing radiometric temperature may be an alternative for future studies that examine data acquired at the same time of day from sites that are in close geographic proximity to avoid the requirement of emissivity or atmospheric correction for validation of results. We foresee future studies using TIR photography to describe species-level thermodynamics that could prove highly valuable toward a better understanding of species-specific responses to climate change in the Arctic

Annual, seasonal, and diel surface energy partitioning in the semiarid Sand Hills of Nebraska, USA

Study Region: The Nebraska Sand Hills consisting of four major land cover types: (1) lakes and wetlands (∼5% for both), (2) subirrigated meadows (∼10%), (3) dry valleys (∼20%), and (4) upland dunes (∼65%). Study Focus: Examination of surface energy and water balances on multiple temporal scales with primary focus on latent heat flux (λE), and evapotranspiration (ET), to gain a better understanding of the annual, seasonal, and diel properties of surface energy partitioning among different Sand Hills ecosystems to improve regional water resource management. New Hydrological Insights for the Region: Based on surface energy and water balance measurements using Bowen ratio energy balance systems at three locations during 2004, we find a strong spatial gradient between sites in latent (λE) and sensible (H) heat flux due to differences in topography, soils, and plant community composition on all timescales. Seasonally, all land covers show the greatest λE in summer. Our results show that subirrigated meadows, dry valleys, and upland dunes allocate roughly 81%, 50%, and 41% of available energy to λE, respectively, during the growing season. The subirrigated meadow was the only cover type where cumulative annual ET surpassed cumulative annual precipitation (i.e. net loss of water to the atmosphere). Therefore, the dry valleys and upland dunes are where net groundwater recharge to the High Plains Aquifer is occurring

Evaporation from a shallow, saline lake in the Nebraska Sandhills: Energy balance drivers of seasonal and interannual variability

Despite potential evaporation rates in excess of the local precipitation, dry climates often support saline lakes through groundwater inputs of water and associated solutes. These groundwater-fed lakes are important indicators of environmental change, in part because their shallow water levels and salinity are very sensitive to weather and climatic variability. Some of this sensitivity arises from high rates of open-water evaporation, which is a dominant but poorly quantified process for saline lakes. This study used the Bowen ratio energy budget method to calculate open-water evaporation rates for Alkali Lake, a saline lake in the Nebraska Sandhills region (central United States), where numerous groundwaterfed lakes occupy the landscape. Evaporation rates were measured during the warm season (May – October) over three consecutive years (2007–2009) to gain insights into the climatic and limnological factors driving evaporation, as well as the partitioning of energy balance components at seasonal and interannual time scales. Results show a seasonal peak in evaporation rate in late June of 7.0 mm day–1 (on average), with a maximum daily rate of 10.5 mm day–1 and a 3-year mean July-September (JAS) rate of 5.1 mm day–1, which greatly exceeds the long-term JAS precipitation rate of 1.3 mm day–1. Seasonal variability in lake evaporation closely follows that of net radiation and lake surface temperature, with sensible heat flux and heat storage variations being relatively small, except in response to short-term, synoptic events. Interannual changes in the surface energy balance were weak, by comparison, although a 6-fold increase in mean lake level over the three years (0.05–0.30 m) led to greater heat storage within the lake, an enhanced JAS lake-air temperature gradient, and greater sensible heat loss. These large variations in water level were also associated with large changes in absolute salinity (from 28 to 118 g kg–1), with periods of high salinity characterized by reductions in mass transfer estimates of evaporation rate by up to 20%, depending on atmospheric conditions and absolute salinity. Energy balance estimates of evaporation, on the other hand, were found to be less sensitive to variations in salinity. These results provide regional insights for lakes in the Nebraska Sandhills region and implications for estimation of the energy and water balance of saline lakes in similar arid and semi-arid landscapes

A review of open top chamber (OTC) performance across the ITEX Network

Open top chambers (OTCs) were adopted as the recommended warming mechanism by the International Tundra Experiment (ITEX) network in the early 1990’s. Since then, OTCs have been deployed across the globe. Hundreds of papers have reported the impacts of OTCs on the abiotic environment and the biota. Here we review the impacts of the OTC on the physical environment, with comments on the appropriateness of using OTCs to characterize the response of biota to warming. The purpose of this review is to guide readers to previously published work and to provide recommendations for continued use of OTCs to understand the implications of warming on low stature ecosystems. In short, the OTC is a useful tool to experimentally manipulate temperature, however the characteristics and magnitude of warming varies greatly in different environments, therefore it is important to document chamber performance to maximize the interpretation of biotic response. When coupled with long-term monitoring, warming experiments are a valuable means to understand the impacts of climate change on natural ecosystems

Interactions between growth-dependent changes in cell size, nutrient supply and cellular elemental stoichiometry of marine Synechococcus

The factors that control elemental ratios within phytoplankton, like carbon:nitrogen:phosphorus (C:N:P), are key to biogeochemical cycles. Previous studies have identified relationships between nutrient-limited growth and elemental ratios in large eukaryotes, but little is known about these interactions in small marine phytoplankton like the globally important Cyanobacteria. To improve our understanding of these interactions in picophytoplankton, we asked how cellular elemental stoichiometry varies as a function of steady-state, N- and P-limited growth in laboratory chemostat cultures of Synechococcus WH8102. By combining empirical data and theoretical modeling, we identified a previously unrecognized factor (growth-dependent variability in cell size) that controls the relationship between nutrient-limited growth and cellular elemental stoichiometry. To predict the cellular elemental stoichiometry of phytoplankton, previous theoretical models rely on the traditional Droop model, which purports that the acquisition of a single limiting nutrient suffices to explain the relationship between a cellular nutrient quota and growth rate. Our study, however, indicates that growth-dependent changes in cell size have an important role in regulating cell nutrient quotas. This key ingredient, along with nutrient-uptake protein regulation, enables our model to predict the cellular elemental stoichiometry of Synechococcus across a range of nutrient-limited conditions. Our analysis also adds to the growth rate hypothesis, suggesting that P-rich biomolecules other than nucleic acids are important drivers of stoichiometric variability in Synechococcus. Lastly, by comparing our data with field observations, our study has important ecological relevance as it provides a framework for understanding and predicting elemental ratios in ocean regions where small phytoplankton like Synechococcus dominates

An Essential Role for DYF-11/MIP-T3 in Assembling Functional Intraflagellar Transport Complexes

MIP-T3 is a human protein found previously to associate with microtubules and the kinesin-interacting neuronal protein DISC1 (Disrupted-in-Schizophrenia 1), but whose cellular function(s) remains unknown. Here we demonstrate that the C. elegans MIP-T3 ortholog DYF-11 is an intraflagellar transport (IFT) protein that plays a critical role in assembling functional kinesin motor-IFT particle complexes. We have cloned a loss of function dyf-11 mutant in which several key components of the IFT machinery, including Kinesin-II, as well as IFT subcomplex A and B proteins, fail to enter ciliary axonemes and/or mislocalize, resulting in compromised ciliary structures and sensory functions, and abnormal lipid accumulation. Analyses in different mutant backgrounds further suggest that DYF-11 functions as a novel component of IFT subcomplex B. Consistent with an evolutionarily conserved cilia-associated role, mammalian MIP-T3 localizes to basal bodies and cilia, and zebrafish mipt3 functions synergistically with the Bardet-Biedl syndrome protein Bbs4 to ensure proper gastrulation, a key cilium- and basal body-dependent developmental process. Our findings therefore implicate MIP-T3 in a previously unknown but critical role in cilium biogenesis and further highlight the emerging role of this organelle in vertebrate development

The Changing Landscape for Stroke\ua0Prevention in AF: Findings From the GLORIA-AF Registry Phase 2

Background GLORIA-AF (Global Registry on Long-Term Oral Antithrombotic Treatment in Patients with Atrial Fibrillation) is a prospective, global registry program describing antithrombotic treatment patterns in patients with newly diagnosed nonvalvular atrial fibrillation at risk of stroke. Phase 2 began when dabigatran, the first non\u2013vitamin K antagonist oral anticoagulant (NOAC), became available. Objectives This study sought to describe phase 2 baseline data and compare these with the pre-NOAC era collected during phase 1. Methods During phase 2, 15,641 consenting patients were enrolled (November 2011 to December 2014); 15,092 were eligible. This pre-specified cross-sectional analysis describes eligible patients\u2019 baseline characteristics. Atrial fibrillation disease characteristics, medical outcomes, and concomitant diseases and medications were collected. Data were analyzed using descriptive statistics. Results Of the total patients, 45.5% were female; median age was 71 (interquartile range: 64, 78) years. Patients were from Europe (47.1%), North America (22.5%), Asia (20.3%), Latin America (6.0%), and the Middle East/Africa (4.0%). Most had high stroke risk (CHA2DS2-VASc [Congestive heart failure, Hypertension, Age  6575 years, Diabetes mellitus, previous Stroke, Vascular disease, Age 65 to 74 years, Sex category] score  652; 86.1%); 13.9% had moderate risk (CHA2DS2-VASc = 1). Overall, 79.9% received oral anticoagulants, of whom 47.6% received NOAC and 32.3% vitamin K antagonists (VKA); 12.1% received antiplatelet agents; 7.8% received no antithrombotic treatment. For comparison, the proportion of phase 1 patients (of N = 1,063 all eligible) prescribed VKA was 32.8%, acetylsalicylic acid 41.7%, and no therapy 20.2%. In Europe in phase 2, treatment with NOAC was more common than VKA (52.3% and 37.8%, respectively); 6.0% of patients received antiplatelet treatment; and 3.8% received no antithrombotic treatment. In North America, 52.1%, 26.2%, and 14.0% of patients received NOAC, VKA, and antiplatelet drugs, respectively; 7.5% received no antithrombotic treatment. NOAC use was less common in Asia (27.7%), where 27.5% of patients received VKA, 25.0% antiplatelet drugs, and 19.8% no antithrombotic treatment. Conclusions The baseline data from GLORIA-AF phase 2 demonstrate that in newly diagnosed nonvalvular atrial fibrillation patients, NOAC have been highly adopted into practice, becoming more frequently prescribed than VKA in Europe and North America. Worldwide, however, a large proportion of patients remain undertreated, particularly in Asia and North America. (Global Registry on Long-Term Oral Antithrombotic Treatment in Patients With Atrial Fibrillation [GLORIA-AF]; NCT01468701