El uso de las TIC como herramienta para la socialización de los recursos didácticos empleados por docentes de Inglés en el Nivel Medio Superior

Este trabajo presenta los resultados de un proyecto de investigación-acción que tiene como objetivo primordial fortalecer el conocimiento del docente de inglés en el Nivel Medio Superior sobre los recursos didácticos empleados en la unidad de aprendizaje de inglés. La propuesta didáctica del estudio propone el uso de las Tecnologías de la Información y la Comunicación (TIC) a través de videos tutoriales como herramienta para socializar, es decir, dar a conocer los aspectos fundamentales de cada recurso didáctico de forma más inmediata y práctica

Deletion of E184L, a Putative DIVA Target from the Pandemic Strain of African Swine Fever Virus, Produces a Reduction in Virulence and Protection against Virulent Challenge

African swine fever (ASF) is currently causing a major pandemic affecting the swine industry and protein availability from Central Europe to East and South Asia. No commercial vaccines are available, making disease control dependent on the elimination of affected animals. Here, we show that the deletion of the African swine fever virus (ASFV) E184L gene from the highly virulent ASFV Georgia 2010 (ASFV-G) isolate produces a reduction in virus virulence during the infection in swine. Of domestic pigs intramuscularly inoculated with a recombinant virus lacking the E184L gene (ASFV-G-ΔE184L), 40% experienced a significantly (5 days) delayed presentation of clinical disease and, overall, had a 60% rate of survival compared to animals inoculated with the virulent parental ASFV-G. Importantly, all animals surviving ASFV-G-ΔE184L infection developed a strong antibody response and were protected when challenged with ASFV-G. As expected, a pool of sera from ASFV-G-ΔE184L-inoculated animals lacked any detectable antibody response to peptides partially representing the E184L protein, while sera from animals inoculated with an efficacious vaccine candidate, ASFV-G-ΔMGF, strongly recognize the same set of peptides. These results support the potential use of the E184L deletion for the development of vaccines able to differentiate infected from vaccinated animals (DIVA). Therefore, it is shown here that the E184L gene is a novel ASFV determinant of virulence that can potentially be used to increase safety in preexisting vaccine candidates, as well as to provide them with DIVA capabilities. To our knowledge, E184L is the first ASFV gene product experimentally shown to be a functional DIVA antigenic marker. IMPORTANCE No commercial vaccines are available to prevent African swine fever (ASF). The ASF pandemic caused by the ASF virus Georgia 2010 (ASFV-G) strain is seriously affecting pork production in a contiguous geographical area from Central Europe to East Asia. The only effective experimental vaccines are viruses attenuated by deleting ASFV genes associated with virus virulence. Therefore, identification of such genes is of critical importance for vaccine development. Here, we report the discovery of a novel determinant of ASFV virulence, the E184L gene. Deletion of the E184L gene from the ASFV-G genome (ASFV-G-ΔE184L) produced a reduction in virus virulence, and importantly, animals surviving infection with ASFV-G-ΔE184L were protected from developing ASF after challenge with the virulent parental virus ASFV-G. Importantly, the virus protein encoded by E184L is highly immunogenic, making a virus lacking this gene a vaccine candidate that allows the differentiation of infected from vaccinated animals (DIVA). Here, we show that unlike what is observed in animals inoculated with the vaccine candidate ASFV-G-ΔMGF, ASFV-G-ΔE184L-inoculated animals do not mount a E184L-specific antibody response, indicating the feasibility of using the E184L deletion as the antigenic marker for the development of a DIVA vaccine in ASFV.info:eu-repo/semantics/acceptedVersio

Proyecto Mikuy fit

Actualmente, bajo la coyuntura producida por el COVID-19 se encontró que los peruanos subieron de peso en promedio 7.7 kilos. Adicionalmente, se encontró que estos se veían influenciados a no reducir de peso por la poca disponibilidad de los especialistas en nutrición y terapias físicas para brindar planes acordes a todos sus requerimientos. Respecto al eje alimenticio, se presentaron dolencias como restricciones dentro de los planes y la poca flexibilización de los alimentos; mientras que, en el eje deportivo, mostraban disconformidad con las rutinas excesivas. Entonces, existía una carencia en la alineación y personalización.  La solución ante este problema fue Mikuy Fit, un aplicativo dirigido a personas entre los 18 a 35 años, pertenecientes a las categorías “A” y “B”. Este aplicativo se enfoca en brindar planes alimenticios y deportivos de manera automatizada, con seguimiento personalizado vía chatbot, el cual adaptará ambos planes según las preferencias de cada usuario. Adicionalmente, utiliza el sistema de geolocalización, enfocado en generar una comunidad donde los usuarios puedan tener un espacio para ejercitarse de manera grupal. También, presenta la funcionalidad de reconocimiento corporal, que permitirá que el usuario pueda ser diagnosticado sobre sus índices iniciales de manera óptima con la cámara del celular. Para ejecutar el proyecto se presentaron planes estratégicos de sistemas, RRHH, marketing y finanzas. Esto pudo determinar que la ejecución del proyecto es viable, pues como resultado se tuvo que la inversión inicial será de S/ 81,494.55 en el primer año de operaciones para obtener la utilidad neta de S/ 16,323.49 para el segundo un total de S/ 103,335.05 y para el tercer año S/ 152,085.57Currently, under the global pandemic caused by COVID-19, it was found that Peruvians gained an average of 7.7 kilos in weight. Additionally, it was found that they were influenced not to lose weight due to the limited availability of specialists in nutrition and physical therapies to provide plans according to all their requirements. Regarding the nutritional axis, there were ailments such as restrictions within the plans and the little flexibility of food; while, on the sports axis, they showed dissatisfaction with excessive routines. So there was a lack of alignment and customization. The solution to this problem was Mikuy Fit, an application aimed at people between 18 to 35 years old, belonging to categories "A" and "B". This application focuses on providing meal and sports plans in an automated way, with personalized monitoring via chatbot, which will adapt both plans according to the preferences of each user. Additionally, it uses the geolocation system, focused on generating a community where users can have a space to exercise as a group. It also presents the body recognition functionality, which will allow the user to be optimally diagnosed on their initial indices with the cell phone camera. To execute the project, strategic plans for systems, HR, marketing and finances were presented. This could determine that the execution of the project is viable, since as a result the initial investment had to be S/ 81,494.55 in the first year of operations to obtain the net profit of S/ 16,323.49 for the second a total of S/ 103,335.05 and for the third year S/ 152,085.57Trabajo de investigació

Cuentos de nunca acabar. Aproximaciones desde la interculturalidad

Cuentos de nunca acabar. Aproximaciones desde la interculturalidad, surge después de la pandemia y su imposibilidad de socializar “en persona” con los compañeros de eventuales encuentros, porque la Comprensión Lectora tenía que reinventarse para su nueva reflexión cognitiva, adaptación contextual y reconstrucción del conocimiento. Este renovado enfoque de la realidad postpandemia, concebido en el marco de la educación intercultural comunitaria, busca potencializar los entornos naturales, sociales y culturales como recursos de aprendizaje multidisciplinario a través del lenguaje animado de los cuentos. En este marco, había que dinamizar la asignatura de Comunicación Oral y Escrita, que se dicta en los Primeros Niveles de los Centros de Apoyo de Otavalo, Cayambe, Latacunga y Riobamba, mediante un eje transversal donde los estudiantes escriban fundamentados en valores de la cosmovisión andina, considerando que provienen de varios lugares de la sierra y amazonía ecuatoriana. Todo surgió del encuentro presencial de un sábado cualquiera donde los estudiantes realizaban ejercicios narrativos, logrando una apreciable respuesta de imaginación, más emotiva que la clásica tarea de las Unidades, tanto así que, pasados unos días, seguían llegando sus escritos a mi correo. Entonces nos pusimos manos a la obra, cada estudiante tendría dos opciones como Actividad Integradora, la primera consistía en escribir un cuento de su propia inspiración, y la segunda analizar un clásico para comentar sus valores y antivalores. La mayor parte de estudiantes decidió escribir su propio cuento, de donde se escogieron algunas participaciones que podrían considerarse originales, para una edición que, respetando la transcripción de la tradición oral que prima en los sectores comunitarios, nos concretamos en revisar la puntuación y ortografía para publicarlos. Con esto buscamos innovar la Actividad Integradora, por algo más práctico y operativo para configurar los Objetos de Aprendizaje que buscamos. Así nació, en medio del camino, este libro de Cuentos de nunca acabar. Aproximaciones desde la interculturalidad, que ponemos en sus manos. Hernán Hermosa Mantilla Quito, junio de 202

Quality of life in older adults from a human settlement in Villa María del Triunfo, during the covid-19 pandemic.

Objetivo: Determinar la calidad de vida en adultos mayores de un asentamiento humano en Villa María del Triunfo, durante la pandemia COVID-19. Materiales y métodos: La investigación fue desarrollada bajo la perspectiva del enfoque cuantitativo y su diseño metodológico fue descriptivo y transversal. La población fue conformada por 105 personas adultas mayores. La técnica fue la encuesta y el instrumento fue el cuestionario WHOQOL-OLD, el cual consta de 24 ítems y 6 dimensiones. Resultados: En cuanto a la calidad de vida, prevaleció el valor medianamente bueno con 75,2% (n=79), seguido del bueno con 15,2% (n=16) y el malo con 9,5% (n=10). En relación a las dimensiones, en capacidad sensorial, prevaleció el valor medianamente bueno con 76,2% (n=80), en autonomía, el valor medianamente bueno con 66,7% (n=70), en actividades, pasadas/presentes/futuras, el valor bueno con 57,1% (n=60), en interacción social, el valor bueno con 57,1% (n=60), en muerte/agonía, el valor medianamente bueno con 30,5% (n=65) y en intimidad, el valor medianamente bueno con 60% (n=63). Conclusiones: En cuanto a la calidad de vida, prevaleció el valor medianamente bueno, seguido del valor bueno y valor malo. Según sus dimensiones, en capacidad sensorial, prevaleció el valor medianamente bueno, en autonomía, prevaleció el valor medianamente bueno, en actividades, pasadas, presentes y futuras, prevaleció el valor bueno, en interacción social, prevaleció el valor bueno, en muerte-agonía, prevaleció el valor medianamente bueno y en intimidad, prevaleció el valor medianamente bueno

ASF Vaccine Candidate ASFV-G-∆I177L Does Not Exhibit Residual Virulence in Long-Term Clinical Studies

African swine fever (ASF) is an important disease in swine currently producing a pandemic affecting pig production worldwide. Except in Vietnam, where two vaccines were recently approved for controlled use in the field, no vaccine is commercially available for disease control. Up to now, the most effective vaccines developed are based on the use of live-attenuated viruses. Most of these promising vaccine candidates were developed by deleting virus genes involved in the process of viral pathogenesis and disease production. Therefore, these vaccine candidates were developed via the genomic modification of parental virus field strains, producing recombinant viruses and reducing or eliminating their residual virulence. In this scenario, it is critical to confirm the absence of any residual virulence in the vaccine candidate. This report describes the assessment of the presence of residual virulence in the ASFV vaccine candidate ASFV-G-∆I177L in clinical studies conducted under high virus loads and long-term observation periods. The results demonstrated that domestic pigs intramuscularly inoculated with 106 HAD50 of ASFV-G-∆I177L did not show the presence of any clinical sign associated with ASF when observed daily either 90 or 180 days after vaccination. In addition, necropsies conducted at the end of the experiment confirmed the absence of macroscopic internal lesions associated with the disease. These results corroborate the safety of using ASFV-G-∆I177L as a vaccine candidate

Evaluation of an ASFV RNA Helicase Gene A859L for Virus Replication and Swine Virulence

African swine fever virus (ASFV) is producing a devastating pandemic that, since 2007, has spread to a contiguous geographical area from central Europe to Asia. In July 2021, ASFV was detected in the Dominican Republic, the first report of the disease in the Americas in more than 40 years. ASFV is a large, highly complex virus harboring a large dsDNA genome that encodes for more than 150 genes. The majority of these genes have not been functionally characterized. Bioinformatics analysis predicts that ASFV gene A859L encodes for an RNA helicase, although its function has not yet been experimentally assessed. Here, we evaluated the role of the A859L gene during virus replication in cell cultures and during infection in swine. For that purpose, a recombinant virus (ASFV-G-∆A859L) harboring a deletion of the A859L gene was developed using the highly virulent ASFV Georgia (ASFV-G) isolate as a template. Recombinant ASFV-G-∆A859L replicates in swine macrophage cultures as efficiently as the parental virus ASFV-G, demonstrating that the A859L gene is non-essential for ASFV replication. Experimental infection of domestic pigs demonstrated that ASFV-G-∆A859L replicates as efficiently and induces a clinical disease indistinguishable from that caused by the parental ASFV-G. These studies conclude that the predicted RNA helicase gene A859L is not involved in the processes of virus replication or disease production in swine

Evaluation of the Function of the ASFV KP177R Gene, Encoding for Structural Protein p22, in the Process of Virus Replication and in Swine Virulence

African swine fever virus (ASFV) causes a devastating disease of swine that has caused outbreaks in Central Europe since 2007, spreading into Asia in 2018. ASFV is a large, structurally complex virus with a large dsDNA genome encoding for more than 160 genes, most of them still uncharacterized. p22, encoded by the ASFV gene KP177R, is an early transcribed, structural virus protein located in the ASFV particle. Although its exact function is unknown, p22 has recently been identified as an interacting partner of several host proteins. Here, we describe the development of a recombinant ASFV (ASFV-G-∆KP177R) lacking the KP177R gene as a tool to evaluate the role of p22 in virus replication and virulence in swine. The recombinant ASFV-G-∆KP177R demonstrated that the KP177R gene is non-essential for ASFV replication in primary swine macrophages, with virus yields similar to those of the parental, highly virulent field isolate Georgia2010 (ASFV-G). In addition, experimental infection of domestic pigs with ASFV-G-∆KP177R produced a clinical disease similar to that caused by the parental ASFV-G. Therefore, and surprisingly, p22 does not seem to be involved in virus replication or virulence in swine

The Presence of Virus Neutralizing Antibodies Is Highly Associated with Protection against Virulent Challenge in Domestic Pigs Immunized with ASFV live Attenuated Vaccine Candidates

African swine fever virus (ASFV) is currently producing a pandemic affecting a large area of Eurasia, and more recently, the Dominican Republic in the Western Hemisphere. ASFV is a large and structurally complex virus with a large dsDNA genome encoding for more than 150 genes. Live attenuated virus strains can induce protection in domestic swine against disease produced by homologous virulent parental viruses. The roles of the different immune mechanisms induced by the attenuated strains in protection still need to be understood. In particular, the role of ASFV neutralizing antibody in protection still is an important controversial issue to be elucidated. Here we present the development of a novel methodology to detect virus neutralizing antibodies based on the reduction of virus infectivity in a Vero cell adapted ASFV strain. The described method was used to assess levels of virus neutralizing antibodies in domestic swine inoculated with live attenuated ASFV. Results demonstrated a high association between the presence of virus neutralizing antibodies and protection in 84 animals immunized with the recombinant vaccine candidates ASFV-G-Δ9GL/ΔUK or ASFV-G-ΔI177L. To our knowledge, this is the first report demonstrating an association between virus neutralizing antibodies and protection against virulent challenge in such a large number of experimental individuals

Full genome sequence for the African swine fever virus outbreak in the Dominican Republic in 1980

Abstract African swine fever is a lethal disease of domestic pigs, geographically expanding as a pandemic, that is affecting countries across Eurasia and severely damaging their swine production industry. After more than 40 years of being absent in the Western hemisphere, in 2020 ASF reappeared in the Dominican Republic and Haiti. The recent outbreak strain in the Dominican Republic has been identified as a genotype II ASFV a derivative of the ASF strain circulating in Asia and Europe. However, to date no full-length genome sequence from either the 1978–1980 Here we report the complete genome sequence of an African swine fever virus (ASFV) (DR-1980) that was previously isolated from blood collected in 1980 from the Dominican Republic at the end of the last outbreak, before culling of all swine on the island of Hispaniola and stored in the Plum Island Animal Disease Center ASFV repository. A contig representing the full-length genome (183,687 base pairs) was de novo assembled into a single contig using both Nanopore and Illumina sequences. DR-1980 was determined to belong to genotype I and, as determined by full genome comparison, a close relative to the sequenced Sardinia viruses that were causing outbreaks at this time