“CLIMA ORGANIZACIONAL EN UN CENTRO COMERCIAL DE LA CIUDAD DE TOLUCA, ESTADO DE MÉXICO, 2012: DIAGNÓSTICO Y ESTRATEGIAS DE MEJORA.”

Largas horas de trabajo, fatiga, malas remuneraciones, estudios y el ejercicio de estos, son algunas de las causas por las que varias empresas se ven en la necesidad de gastar gran parte de sus ingresos en la contratación de nuevo personal. Ante este problema se han generado ideas rígidas que extralimitan el funcionamiento del cuerpo y lo adhieren a las maquinas, estructuras formales y cerradas que entorpecen la comunicación, el entendimiento del mundo a partir de la organización de la sociedad, desarrollo del trabajo iniciando con la comprensión de la psicología del trabajador, la manera en que este pueda entenderse con los demás y consigo mismo para poder formar parte de la organización donde será integrado y motivado; son algunas de las teorías que a lo largo de la historia se han formado alrededor de la desarrollo organizacional

Influence of Serum Vitamin D Level in the Response of Actinic Keratosis to Photodynamic Therapy with Methylaminolevulinate

In mouse models of squamous cell carcinoma, pre-treatment with calcitriol prior to photodynamic therapy with aminolevulinic acid (ALA) enhances tumor cell death. We have evaluated the association between vitamin D status and the response of actinic keratoses to photodynamic therapy with methylaminolevulinate. Twenty-five patients with actinic keratoses on the head received one session of photodynamic therapy with methylaminolevulinate. Biopsies were taken at baseline and six weeks after treatment. Immuno-histochemical staining was performed for VDR, P53, Ki67 and beta-catenin. Basal serum 25(OH)D levels were determined. Cases with a positive histological response to treatment had significantly higher serum 25(OH)D levels (26.96 (SD 7.49) ngr/mL) than those without response (18.60 (SE 7.49) ngr/mL) (p = 0.05). Patients with a complete clinical response displayed lower basal VDR expression (35.71% (SD 19.88)) than partial responders (62.78% (SD 16.735)), (p = 0.002). Our results support a relationship between vitamin D status and the response of actinic keratoses to photodynamic therapy with methylaminolevulinate

MOTIVACIÓN AL PERSONAL DE VENTAS A TRAVÉS DE UN ESQUEMA DE COMPENSACIÓN VARIABLE

La presente investigación se realizó entre los meses de Junio a Agosto del 2016. El objetivo de la misma fue analizar la relación de la motivación en el personal del departamento de ventas en una empresa embotelladora de la ciudad de Chihuahua, con respecto a la aplicación de un esquema de compensación variable. Los criterios metodológicos utilizados fueron, métodos: analítico-sintético y teórico-deductivo, tipo descriptivo, modo bibliográfico y de campo. La población de interés fueron cuarenta empleados del departamento de ventas. A principios del año 2016, la empresa optó por la implementación de un esquema de compensación variable, porque cuenta con la estructura necesaria y poco personal, lo que facilita este modelo de plan de ventas. El esquema de compensación variable funciona básicamente a través de una disminución del salario fijo y un aumento de salario en la base variable, esto lleva a que el vendedor se mantenga continuamente pretendiendo alcanzar mayores volúmenes de ventas. La importancia de esta investigación radicó en el estudio de la influencia del sistema de compensación variable sobre la motivación y las ventas de los empleados. Los principales resultados arrojaron que los incentivos económicos proporcionados por el esquema de compensación variable sí ayudan a los empleados a satisfacer sus necesidades elementales de vivienda, alimentación, pago de servicios y de transporte; también les proporciona mayor seguridad económica, aumentando su autoestima personal y autorrealización profesional, lo que permitirá incluir este tipo de compensación en las sucursales de todo el país

Within Subject Cross-Tissue Analyzes of Epigenetic Clocks in Substance Use Disorder Postmortem Brain and Blood

There is a possible accelerated biological aging in patients with substance use disorders (SUD). The evaluation of epigenetic clocks, which are accurate estimators of biological aging based on DNA methylation changes, has been limited to blood tissue in patients with SUD. Consequently, the impact of biological aging in the brain of individuals with SUD remains unknown. In this study, we evaluated multiple epigenetic clocks (DNAmAge, DNAmAgeHannum, DNAmAgeSkinBlood, DNAmPhenoAge, DNAmGrimAge, and DNAmTL) in individuals with SUD (n = 42), including alcohol (n = 10), opioid (n = 19), and stimulant use disorder (n = 13), and controls (n = 10) in postmortem brain (prefrontal cortex) and blood tissue obtained from the same individuals. We found a higher DNAmPhenoAge (β = 0.191, p-value = 0.0104) and a nominally lower DNAmTL (β = -0.149, p-value = 0.0603) in blood from individuals with SUD compared to controls. SUD subgroup analysis showed a nominally lower brain DNAmTL in subjects with alcohol use disorder, compared to stimulant use disorder and controls (β = 0.0150, p-value = 0.087). Cross-tissue analyzes indicated a lower blood DNAmTL and a higher blood DNAmAge compared to their respective brain values in the SUD group. This study highlights the relevance of tissue specificity in biological aging studies and suggests that peripheral measures of epigenetic clocks in SUD may depend on the specific type of drug used

Within Subject Cross-Tissue Analyzes of Epigenetic Clocks in Substance Use Disorder Postmortem Brain and Blood.

There is a possible accelerated biological aging in patients with substance use disorders (SUD). The evaluation of epigenetic clocks, which are accurate estimators of biological aging based on DNA methylation changes, has been limited to blood tissue in patients with SUD. Consequently, the impact of biological aging in the brain of individuals with SUD remains unknown. In this study, we evaluated multiple epigenetic clocks (DNAmAge, DNAmAgeHannum, DNAmAgeSkinBlood, DNAmPhenoAge, DNAmGrimAge, and DNAmTL) in individuals with SUD (n=42), including alcohol (n=10), opioid (n=19), and stimulant use disorder (n=13), and controls (n=10) in postmortem brain (prefrontal cortex) and blood tissue obtained from the same individuals. We found a higher DNAmPhenoAge (beta=0.191, p-value=0.0104) and a nominally lower DNAmTL (beta=−0.149, p-value=0.0603) in blood from individuals with SUD compared to controls. SUD subgroup analysis showed a nominally lower brain DNAmTL in subjects with alcohol use disorder, compared to stimulant use disorder and controls (beta=0.0150, p-value=0.087). Cross-tissue analyses indicated a lower blood DNAmTL and a higher blood DNAmAge compared to their respective brain values in the SUD group. This study highlights the relevance of tissue specificity in biological aging studies and suggests that peripheral measures of epigenetic clocks in SUD may depend on the specific type of drug used

A Human Stem Cell-Derived Neuronal Model of Morphine Exposure Reflects Brain Dysregulation in Opioid Use Disorder: Transcriptomic and Epigenetic Characterization of Postmortem-Derived iPSC Neurons

INTRODUCTION: Human-derived induced pluripotent stem cell (iPSC) models of brain promise to advance our understanding of neurotoxic consequences of drug use. However, how well these models recapitulate the actual genomic landscape and cell function, as well as the drug-induced alterations, remains to be established. New METHODS: We engineered a novel induced pluripotent stem cell-derived model of neural progenitor cells and neurons from cultured postmortem human skin fibroblasts, and directly compared these to isogenic brain tissue from the donor source. We assessed the maturity of the cell models across differentiation from stem cells to neurons using RNA cell type and maturity deconvolution analyses as well as DNA methylation epigenetic clocks trained on adult and fetal human tissue. As proof-of-concept of this model\u27s utility for substance use disorder studies, we compared morphine- and cocaine-treated neurons to gene expression signatures in postmortem Opioid Use Disorder (OUD) and Cocaine Use Disorder (CUD) brains, respectively. RESULTS: Within each human subject (N = 2, 2 clones each), brain frontal cortex epigenetic age parallels that of skin fibroblasts and closely approximates the donor\u27s chronological age; stem cell induction from fibroblast cells effectively sets the epigenetic clock to an embryonic age; and differentiation of stem cells to neural progenitor cells and then to neurons progressively matures the cells DISCUSSION: In summary, we introduce an iPSC model generated from human postmortem fibroblasts that can be directly compared to corresponding isogenic brain tissue and can be used to model perturbagen exposure such as that seen in opioid use disorder. Future studies with this and other postmortem-derived brain cellular models, including cerebral organoids, can be an invaluable tool for understanding mechanisms of drug-induced brain alterations

Analysis of the metabolic proteome of lung adenocarcinomas by reverse-phase protein arrays (RPPA) emphasizes mitochondria as targets for therapy

Lung cancer is the leading cause of cancer-related death worldwide despite the success of therapies targeting oncogenic drivers and immune-checkpoint inhibitors. Although metabolic enzymes offer additional targets for therapy, the precise metabolic proteome of lung adenocarcinomas is unknown, hampering its clinical translation. Herein, we used Reverse Phase Protein Arrays to quantify the changes in enzymes of glycolysis, oxidation of pyruvate, fatty acid metabolism, oxidative phosphorylation, antioxidant response and protein oxidative damage in 128 tumors and paired non-tumor adjacent tissue of lung adenocarcinomas to profile the proteome of metabolism. Steady-state levels of mitochondrial proteins of fatty acid oxidation, oxidative phosphorylation and of the antioxidant response are independent predictors of survival and/or of disease recurrence in lung adenocarcinoma patients. Next, we addressed the mechanisms by which the overexpression of ATPase Inhibitory Factor 1, the physiological inhibitor of oxidative phosphorylation, which is an independent predictor of disease recurrence, prevents metastatic disease. We highlight that IF1 overexpression promotes a more vulnerable and less invasive phenotype in lung adenocarcinoma cells. Finally, and as proof of concept, the therapeutic potential of targeting fatty acid assimilation or oxidation in combination with an inhibitor of oxidative phosphorylation was studied in mice bearing lung adenocarcinomas. The results revealed that this therapeutic approach significantly extended the lifespan and provided better welfare to mice than cisplatin treatments, supporting mitochondrial activities as targets of therapy in lung adenocarcinoma patientsThe work was supported by grants from MINECO (SAF2016–75916-R and PID2019–108674RB-100), CIBERER-ISCIII (CB06/07/0017) and Fundación Ramón Areces, Spai

Development of a Kemp\u27s Ridley Sea Turtle Stock Assessment Model

We developed a Kemp’s ridley (Lepidochelys kempii) stock assessment model to evaluate the relative contributions of conservation efforts and other factors toward this critically endangered species’ recovery. The Kemp’s ridley demographic model developed by the Turtle Expert Working Group (TEWG) in 1998 and 2000 and updated for the binational recovery plan in 2011 was modified for use as our base model. The TEWG model uses indices of the annual reproductive population (number of nests) and hatchling recruitment to predict future annual numbers of nests on the basis of a series of assumptions regarding age and maturity, remigration interval, sex ratios, nests per female, juvenile mortality, and a putative ‘‘turtle excluder device effect’’ multiplier starting in 1990. This multiplier was necessary to fit the number of nests observed in 1990 and later. We added the effects of shrimping effort directly, modified by habitat weightings, as a proxy for all sources of anthropogenic mortality. Additional data included in our model were incremental growth of Kemp’s ridleys marked and recaptured in the Gulf of Mexico, and the length frequency of stranded Kemp’s ridleys. We also added a 2010 mortality factor that was necessary to fit the number of nests for 2010 and later (2011 and 2012). Last, we used an empirical basis for estimating natural mortality, on the basis of a Lorenzen mortality curve and growth estimates. Although our model generated reasonable estimates of annual total turtle deaths attributable to shrimp trawling, as well as additional deaths due to undetermined anthropogenic causes in 2010, we were unable to provide a clear explanation for the observed increase in the number of stranded Kemp’s ridleys in recent years, and subsequent disruption of the species’ exponential growth since the 2009 nesting season. Our consensus is that expanded data collection at the nesting beaches is needed and of high priority, and that 2015 be targeted for the next stock assessment to evaluate the 2010 event using more recent nesting and in-water data

INNOVA Research Journal

La clase de matemáticas está en un proceso de evolución, las clases tradicionales centradas en el profesor van perdiendo vigencia en la educación ecuatoriana, dando paso a clases que priorizan al estudiante y sus aprendizajes. Tradicionalmente el docente de matemáticas ha tenido formación profesional en áreas técnicas y poca capacitación en aspectos pedagógicos que le den habilidades para llevar adelante una clase fundada en paradigmas constructivistas del aprendizaje, centrados en el estudiante y la calidad de sus aprendizajes. Una clase constructivista de matemáticas implica tener un estudiante con una visión diferente a su formación tradicional, debe estar motivado por alcanzar aprendizajes significativos, para esto debe ser capaz de investigar, analizar, reflexionar, plantear soluciones creativas, resolver situaciones del contexto, formado en valores y que aporta al crecimiento de la sociedad

Asociación entre Artritis Reumatoidea y otras enfermedades autoinmunes

Objetivos: determinar la frecuencia de enfermedades autoinmunes (EAI) en pacientes con Artritis Reumatoidea (AR) y comparar la frecuencia de EAI entre pacientes con AR y sin AR ni otra EAI reumatológica. Material y Métodos: estudio multicéntrico, observacional, analítico, retrospectivo. Se incluyeron pacientes consecutivos con AR (ACR/EULAR 2010) y como grupo control pacientes con diagnóstico inicial de Osteoartritis primaria (OA).