Future prospects for ethanol fuel use - a review

Countries inspired by a desire to reduce greenhouse gas emissions in order to meet their Kyoto Protocol targets, have turned to ethanol fuels as a cheap and proven alternative to reduce vehicular emissions. Political instability in the Middle East has further motivated countries to develop their own fuel supply to ensure the security of supply and promote internal economic growth. The use of fuel ethanol has attracted considerable negative press and public comments in the early part of 2000s. Because of the importance of ethanol fuel as alternative to petrol, this review presents discussions outlining the various benefits and costs of using fuel ethanol with the objective of highlighting the future prospects of its use to reduce greenhouse gas emissions, provide cheap energy source for vehicles and also provide income to farmers producing bio-ethanol. Key words: ethanol fuel, future prospects, conflicting need

CD4+ T-Lymphocyte counts among under-5 children with protein-energy malnutrition as seen in Usmanu Danfodiyo University Teaching Hospital , Sokoto, Nigeria

Background: Proteinenergy malnutrition is a prevalent public health  problem in the developing countries. It affects body systems including cell-mediated immunity.Objectives: To determine the effect of PEM on CD4+ Tlymphocyte counts among under -5 children.Methods: This was a prospective cross-sectional study conducted among HIV-negative children aged 6 - 59 months with PEM and the HIV-negative well-nourished children between November 1st, 2010 and July 31st, 2011. The socio-demographic characteristics, weight and some haematologicalindices of the both groups were documented. The CD4+ Tlymphocyte count was determined using Partec cytoflow machine.Result: One-hundred children were recruited for each group over a 9 month period. The two study groups were comparable in age (p= 0.53) and sex (p= 0.65). The mean CD4+ T-lymphocyte count in children with PEM was1705.5±605.6 cells/µL as compared to 2314.3±491.1 cells/µL among the controls (p= 0.0001). A statistically significant difference was observed in the mean CD4+ T-lymphocyte count of the different types of PEM with the highest value observed among children with kwashiorkor (2097.7±712.9 cells/µL) and lowest value observed among those with marasmus  (1449.3±368.2 cells/µL). There were significant differences in the mean CD4+ Tlymphocyte count of the control (2314.3±491 cells/µL) when  compared to those of marasmus (1449±368 cells/µL) (p= 0.001), marasmic-kwashiorkor (1888±762 cells/µL) (p= 0.002), underweight (1559±452 cells/µL) (p= 0.001) and underweight-kwashiorkor (1534±402 cells/µL) (p= 0.001), but it was comparable with that of kwashiorkor group (2098±713 cells/µL) (p= 0.21). A statistically significant difference was observed in the mean CD4+ Tlymphocyte count of the different types of PEM with the highest value observed among children with kwashiorkor (2097.7±712.9 cells/µL) and lowest value observed among those with  marasmus (1449.3±368.2 cells/µL).Conclusion: The PEM has deleterious effects on the CD4+ Tlymphocytecounts among under-5 children with PEM with the lowest count observed among those presenting with marasmus.Key words: CD4+ T-Lymphocyte, Count, PEM, Under-5

Prevalence of HIV-infection among under-5 children with protein energy malnutrition presenting at Usmanu Danfodiyo University Teaching Hospital, Sokoto, Nigeria

Background: HIV infection is a major health problem worldwide. It is  associated with Protein-Energy Malnutrition (PEM) among under-5 childrenwith attendant high morbidity and mortality.Objective: To determine the prevalence of HIV-infection among children presenting withvarious subtypes of PEM.Methodology: Children suffering from PEM aged below 5 years admitted into the Paediatric units of UDUTH, Sokoto between October 1st, 2010 and April 30th, 2011 were tested for HIV infection using ELISA tests and HIVDNAPCR. Nutritional status was determined using the modified Wellcome Classification and socioeconomic classification was by the scheme developed by Oyedeji,s. Data were analyzed using SPSS 17.0 statistical package. P-value . 0.05 was considered significant.Results: One-hundred under-5 children (64 males, 36 females) with PEM were studied. The mean (±SD) age was 19.8&#177 9.2 months and the majority were aged 12.0-23.9 months. Twentyseven of the 100 children withPEM had HIV-infection giving a prevalence rate of 27%: 59.3% in males and 40.7% in females. Among the HIV-infected children, the 24.0 . 35.9 months age group was the most affected (53.8%). Infected and non-infected children were comparable in terms of age (χ2=7.35, p=0.12) , gender (χ2=0.36, p=0.55) and socioeconomic (χ2=3.01, p=0.25). Themode of transmission was maternal to child transmission in all cases. The highest prevalence of HIV infection was found among marasmus subgroup (65%). Twenty-two (81.5%) of the 27 cases were discharged home, whilefive patients died giving a case fatality rate of 18.5%.Conclusion: HIV infection is common among under-5 children with PEM with no age, gender or socioeconomic predilection. The clinical type of PEM most often affected is marasmus.Key words: Protein-energy malnutrition, HIV-infection, Under-

Transcriptomic profiling disclosed the role of DNA methylation and histone modifications in tumor-infiltrating myeloid-derived suppressor cell subsets in colorectal cancer

Increased numbers of myeloid-derived suppressor cells (MDSCs) are positively correlated with poor prognosis and reduced survivals of cancer patients. They play central roles in tumor immune evasion and tumor metastasis. However, limited data are available on phenotypic/transcriptomic characteristics of the different MDSCs subsets in cancer. These cells include immature (I-MDSCs), monocytic (M-MDSCs), and polymorphonuclear/granulocytic (PMN-MDSCs). Phenotypic characterization of myeloid subsets from 27 colorectal cancer (CRC) patients was assessed by flow cytometric analyses. RNA-sequencing of sorted I-MDSCs, PMN-MDSCs, and antigen-presenting cells (APCs) was also performed. We found that the levels of I-MDSCs and PMN-MDSCs were increased in tumor tissues (TT), compared with normal tissues (NT) in colorectal cancer. Our functional annotation analyses showed that genes associated with histone deacetylase (HDAC) activation- and DNA methylation-mediated transcriptional silencing were upregulated, and histone acetyl transferase (HAT)-related genes were downregulated in tumor-infiltrating I-MDSCs. Moreover, pathways implicated in cell trafficking and immune suppression, including Wnt, interleukin-6 (IL-6), and mitogen-activated protein kinase (MAPK) signaling, were upregulated in I-MDSCs. Notably, PMN-MDSCs showed downregulation in genes related to DNA methylation and HDAC binding. Using an ex vivo model, we found that inhibition of HDAC activation or neutralization of IL-6 in CRC tumor tissues downregulates the expression of genes associated with immunosuppression and myeloid cell chemotaxis, confirming the importance of HDAC activation and IL-6 signaling pathway in MDSC function and chemotaxis. This study provides novel insights into the epigenetic regulations and other molecular pathways in different myeloid cell subsets within the CRC tumor microenvironment (TME), giving opportunities to potential targets for therapeutic benefits

Transcriptomic profiling of tumor-infiltrating CD4 + TIM-3 + T Cells reveals their suppressive, exhausted, and metastatic characteristics in colorectal cancer patients

T cell immunoglobulin mucin-3 (TIM-3) is an immune checkpoint identified as one of the key players in regulating T-cell responses. Studies have shown that TIM-3 is upregulated in the tumor microenvironment (TME). However, the precise role of TIM-3 in colorectal cancer (CRC) TME is yet to be elucidated. We performed phenotypic and molecular characterization of TIM-3+ T cells in the TME and circulation of CRC patients by analyzing tumor tissues (TT, TILs), normal tissues (NT, NILs), and peripheral blood mononuclear cells (PBMC). TIM-3 was upregulated on both CD4+ and CD3+CD4− (CD8+) TILs. CD4+TIM-3+ TILs expressed higher levels of T regulatory cell (Tregs)-signature genes, including FoxP3 and Helios, compared with their TIM-3− counterparts. Transcriptomic and ingenuity pathway analyses showed that TIM-3 potentially activates inflammatory and tumor metastatic pathways. Moreover, NF-κB-mediated transcription factors were upregulated in CD4+TIM-3+ TILs, which could favor proliferation/invasion and induce inflammatory and T-cell exhaustion pathways. In addition, we found that CD4+TIM-3+ TILs potentially support tumor invasion and metastasis, compared with conventional CD4+CD25+ Tregs in the CRC TME. However, functional studies are warranted to support these findings. In conclusion, this study discloses some of the functional pathways of TIM-3+ TILs, which could improve their targeting in more specific therapeutic approaches in CRC patients

Effect of Bone Marrow-derived Mesenchymal Stem Cells and Umbilical Cord Blood-CD34+ cells on Experimental Rat liver Fibrosis

Background and Objective: Liver disease is one of the major causes of death in many countries. Hence, the development of effective therapies for liver fibrosis is a major aim of medical research. So this study was designed to investigate the therapeutical role of mesenchymal stem cells (MSCs) and hematopoietic stem cells (HSCs) transplantation in the experimental rat liver fibrosis. Design and Method: Bone marrow-derived MSCs were isolated from femoral and tibial bones of male albino rats, then were grown and propagated in culture for 2 weeks and were characterized morphologically and by detection of CD29 by real time-PCR. Human umbilical cord blood cells were obtained after full-term caesarean delivery from healthy donors after written informed consent. Low-density mononuclear cells were separated over Ficoll- Paque (Gibco-Invitrogen, Grand Island, NY), and then CD34+ HSC was isolated using a magnetic cell sorter (MiniMACS; Miltenyi Biotec, Bergisch Gladbach, Germany). The cells were then infused intraperitoneally in rats that received CCl4 injection to induce liver fibrosis. Rats were divided into 4 groups: control, CCl4, CCl4 plus MSC, and CCl4 plus CD34+. Liver tissue was examined histopathologically for all groups. The expression of collagen I and metalloproteinase-2 genes as a marker of liver fibrosis was measured by real time RT- PCR. Results: The results of the present study showed that both MSCs and CD34+ have a significant antifibrotic effect as evidenced by the significant decrease in liver collagen gene expression as well as the decrease in MMP-2 (p < 0.05) compared to the CCl4 group

An Association of Prolactin Gene Polymorphisms with Some Milk Traits in Women

We investigated the effects of prolactin gene polymorphisms on milk contents in women. The main aim of this work is to determine the genotypes of prolactin and its relationship with some milk chemical contents in women. Genotyping was carried out at Molecular Genetic Laboratory at the College of Agriculture, whereas biochemical assays were performed at the Department of Diseases Analyses at the South Technical University. Blood samples were collected for the prolactin-related gene. DNA was extracted from fifty candidate women. The extra-pituitary prolactin gene promoter 1149 G/T was subjected to XapI restriction enzyme. In this analysis   PRL-Pxa, I products result in three genotypes TT, TG and GG, as well as the population, is under Hardy-Weinberg equilibrium. Our results showed that the highest milk fat yield, milk protein, lactose and sold not fat (SNF) materials percentages were obtained by the genotype GG

Spect perfusion imaging versus CT for predicting radiation injury to normal lung in lung cancer patients.

Objectives In non-small cell lung cancer (NSCLC) patients, to establish whether the fractional volumes of irradiated anatomic or perfused lung differed between those with and without deteriorating lung function or radiation associated lung injury (RALI).Methods 48 patients undergoing radical radiotherapy for NSCLC had a radiotherapy-planning CT scan and single photon emission CT lung perfusion imaging (99mTc-labelled macroaggregate albumin). CT defined the anatomic and the single photon emission CT scan (co-registered with CT) identified the perfused (threshold 20 % of maximum) lung volumes. Fractional volumes of anatomic and perfused lung receiving more than 5, 10, 13, 20, 30, 40, 50 Gy were compared between patients with deteriorating (>median decline) vs stable (vs stable FEV1 ( p = 0.005, 0.005 and 0.025 respectively) but did not differ for higher doses of radiation (>30, 40, 50 Gy). Fractional volumes of anatomic and perfused lung receiving > 10 Gy best predicted decline in FEV1 (Area under receiver operating characteristic curve (Az = 0.77 and 0.76 respectively); sensitivity/specificity 75%/81 and 80%/71%) for a 32.7% anatomic and 33.5% perfused volume cut-off. Irradiating an anatomic fractional volume of 4.7% to > 50 Gy had a sensitivity/specificity of 83%/89 % for indicating RALI (Az = 0.83).Conclusion A 10-20 Gy radiation dose to anatomic or perfused lung results in decline in FEV1. A fractional anatomic volume of >5% receiving >50 Gy influences development of RALI.Advances in knowledge Extent of low-dose radiation to normal lung influences functional respiratory decline

PREVALENCE AND CLINICAL FORMS OF MALARIA AMONG FEBRILE HIV-INFECTED CHILDREN SEEN AT USMANU DANFODIYO UNIVERSITY TEACHING HOSPITAL, SOKOTO, NIGERIA

Background: Malaria and HIV infections are major health problems facing the world today. Sub-Saharan Africa with 10 percent of world’s population harbors more than half the burden of the scourge. The present study determined the prevalence and clinical forms of malaria among febrile HIV-infected children aged 3months to 15years, seen in Usmanu Danfodiyo University Teaching Hospital (UDUTH), Sokoto, Nigeria. Materials and Methods: Cross-sectional study among febrile HIV-infected children and their control cohort were carried out between May and October 2016. The participants had the following investigations: malarial parasite, packed cell volume, random blood sugar, retroviral test. &nbsp;Results: A total of 140 febrile HIV-infected children aged 3 months to 15 years and 140 febrile HIV-negative age- and gender-matched children were recruited; 100 of the HIV-infected children were on ART and cotrimoxazole. The prevalence of malaria among the febrile HIV-infected children was 71.4% (100/140) which was significantly lower than the prevalence of 94.3% (132/140) among the control group (χ2 27.72, p=0.001). Among the febrile HIV-infected children that had malaria, 54(54.0%) had uncomplicated malaria while 46(46.0%) had severe malaria. Of the 132 controls that had malaria, 48(36.4%) had uncomplicated malaria and 84(63.6%) had severe malaria (χ2 =7.184, p=0.007). Conclusion: Malaria is a problem in HIV-infected children. Since nearly half of the febrile HIV-infected children had severe form of malaria, it is recommended that health promotion, intermittent malaria prophylaxis, early diagnosis and prompt effective treatment should be instituted for HIV-infected children. This may prevent severe form of malaria and its attendant mortality