The Identification of Scientific Programs to Utilize the Space Environment

A program to identify and develop ideas for scientific experimentation on the long duration exposure facility (LDEF) was completed. Four research proposals were developed: (1) Ultra pure germanium gamma ray radiation detectors in the space environment, intended to develop and demonstrate an X-ray and gamma-ray spectroscopy system incorporating a temperature cyclable high-purity germanium detector and diode heat pipe cryogenic system for cooling, (2) growth, morphogenesis and metabolism of plant embryos in the zero-gravity environment, to investigate if the space environment induces mutations in the embryogenic cells so that mutants of commercial significance with desirable attributes may be obtained, (3) effect of zero gravity on the growth and pathogenicity of selected zoopathic fungi. It is possible that new kinds of treatment for candidiasis, and tichophytosis could eventuate from the results of the proposed studies, and (4) importance of gravity to survival strategies of small animals. Gravitational effects may be direct or mediate the selection of genetic variants that are preadapted to weightlessness

Temperature measurements behind reflected shock waves in air

A radiometric method for the measurement of gas temperature in self-absorbing gases has been applied in the study of shock tube generated flows. This method involves making two absolute intensity measurements at identical wavelengths, but for two different pathlengths in the same gas sample. Experimental results are presented for reflected shock waves in air at conditions corresponding to incident shock velocities from 7 to 10 km/s and an initial driven tube pressure of 1 torr. These results indicate that, with this technique, temperature measurements with an accuracy of + or - 5 percent can be carried out. The results also suggest certain facility related problems

DIII-D power supply, design, and development

An overview of the DIII-D power supply system with information details concerning the configuration, power ratings, acquisition costs, and cost scaling relevant to the design of ITER and other tokamaks is presented. The power supplies for the DIII-D tokamak were installed and commissioned during the late 1970`s and the beginning of the 1980`s. Several upgrades have been implemented during the last two years to solve increasing reliability problems encountered as the equipment aged, to provide enhanced operational flexibilities, and to enable operation at the higher power levels needed to provide experimental data relevant to the ITER and TPX design activities. These upgrades ranged from redesign of the power supply control systems to the replacement of vacuum circuit breakers which had become unreliable in service. A new interlock and protection system has also been implemented using the latest programmable logic controllers (PLC) and computer technology. These upgrades have been highly successful and are described to provide insight to many issues in the specification of high power converters. Power supply models used in the design of the DIII-D Plasma Control System are also described along with model verification test data. These models are being used in the development of a new advanced plasma control system for the DIII-D tokamak. Recent operational experience and results are presented

An Ongoing Shift in Pacific Ocean Sea Level

Based on the satellite altimeter data, sea level off the west coast of the United States has increased over the past 5 years, while sea level in the western tropical Pacific has declined. Understanding whether this is a short‐term shift or the beginning of a longer‐term change in sea level has important implications for coastal planning efforts in the coming decades. Here, we identify and quantify the recent shift in Pacific Ocean sea level, and also seek to describe the variability in a manner consistent with recent descriptions of El Nino‐Southern Oscillation (ENSO) and particularly the Pacific Decadal Oscillation (PDO). More specifically, we extract two dominant modes of sea level variability, one related to the biennial oscillation associated with ENSO and the other representative of lower‐frequency variability with a strong signal in the northern Pacific. We rely on cyclostationary empirical orthogonal function (CSEOF) analysis along with sea level reconstructions to describe these modes and provide historical context for the recent sea level changes observed in the Pacific. As a result, we find that a shift in sea level has occurred in the Pacific Ocean over the past few years that will likely persist in the coming years, leading to substantially higher sea level off the west coast of the United States and lower sea level in the western tropical Pacific. Sea level in the Pacific has undergone a shift in the past 5 years, with sea level in the eastern (western) Pacific rising (falling) Sea level variability in the Pacific Ocean has been separated into a biennial oscillation mode and a decadal mode This shift appears to result from a change of phase of a low‐frequency climate signal, that could continue on for the next several year

Improving plasma shaping accuracy through consolidation of control model maintenance, diagnostic calibration, and hardware change control

With the advent of more sophisticated techniques for control of tokamak plasmas comes the requirement for increasingly more accurate models of plasma processes and tokamak systems. Development of accurate models for DIII-D power systems, vessel, and poloidal coils is already complete, while work continues in development of general plasma response modeling techniques. Increased accuracy in estimates of parameters to be controlled is also required. It is important to ensure that errors in supporting systems such as diagnostic and command circuits do not limit the accuracy of plasma parameter estimates or inhibit the ability to derive accurate plasma/tokamak system models. To address this issue, we have developed more formal power systems change control and power system/magnetic diagnostics calibration procedures. This paper discusses our approach to consolidating the tasks in these closely related areas. This includes, for example, defining criteria for when diagnostics should be re-calibrated along with required calibration tolerances, and implementing methods for tracking power systems hardware modifications and the resultant changes to control models

Co-Culture of Endothelial Cells and Smooth Muscle Cells in a Flow Environment: An Improved Culture Model of the Vascular Wall?

Numerous studies have demonstrated that the neighboring smooth muscle cells (SMC) influence the morphology, cytoskeleton and growth of co-cultured endothelial cells (EC). Also, flow-induced laminar shear stress has been shown to induce cell elongation, F-actin reorganization and growth inhibition in cultured EC. We investigated the effect of neighboring SMC and collagen matrix on the response of EC to shear stress. The co-culture system was made by growing porcine aortic SMC in a gel of collagen type I and then seeding porcine aortic EC (P AEC) on the top surface. Then the co-culture was exposed to steady, laminar shear stress of 10 and 30 dynes/cm2 in a parallel-plate flow chamber. EC had a different morphology when cultured on top of collagen gels as compared to cells grown on plastic. When grown in static co-culture with SMC, EC were already elongated and showed a random wavy pattern of orientation. When exposed to 30 dynes/cm2, the EC aligned with the direction of flow after 24 to 48 hours. We suggest that the elongation and orientation of the EC, when cultured on a collagen matrix under static conditions, may be due to contact guidance on the collagen fibers previously rearranged by the SMC during gel retraction. Shear stress, however, was sufficient to induce cell orientation along the direction of flow

Origin of interannual variability in global mean sea level

Author Posting. © National Academy of Sciences, 2020. This article is posted here by permission of National Academy of Sciences for personal use, not for redistribution. The definitive version was published in Proceedings of the National Academy of Sciences of the United States of America 117(25), (2020): 13983-13990, doi: 10.1073/pnas.1922190117.The two dominant drivers of the global mean sea level (GMSL) variability at interannual timescales are steric changes due to changes in ocean heat content and barystatic changes due to the exchange of water mass between land and ocean. With Gravity Recovery and Climate Experiment (GRACE) satellites and Argo profiling floats, it has been possible to measure the relative steric and barystatic contributions to GMSL since 2004. While efforts to “close the GMSL budget” with satellite altimetry and other observing systems have been largely successful with regards to trends, the short time period covered by these records prohibits a full understanding of the drivers of interannual to decadal variability in GMSL. One particular area of focus is the link between variations in the El Niño−Southern Oscillation (ENSO) and GMSL. Recent literature disagrees on the relative importance of steric and barystatic contributions to interannual to decadal variability in GMSL. Here, we use a multivariate data analysis technique to estimate variability in barystatic and steric contributions to GMSL back to 1982. These independent estimates explain most of the observed interannual variability in satellite altimeter-measured GMSL. Both processes, which are highly correlated with ENSO variations, contribute about equally to observed interannual GMSL variability. A theoretical scaling analysis corroborates the observational results. The improved understanding of the origins of interannual variability in GMSL has important implications for our understanding of long-term trends in sea level, the hydrological cycle, and the planet’s radiation imbalance.The research was carried out at JPL, California Institute of Technology, under a contract with NASA. This study was funded by NASA Grants NNX17AH35G (Ocean Surface Topography Science Team), 80NSSC17K0564, and 80NSSC17K0565 (NASA Sea Level Change Team). The efforts of J.T.F. in this work were also supported by NSF Award AGS-1419571, and by the Regional and Global Model Analysis component of the Earth and Environmental System Modeling Program of the US Department of Energy's Office of Biological & Environmental Research via National Science Foundation Grant IA 1844590. C.G.P. was supported by the J. Lamar Worzel Assistant Scientist Fund and the Penzance Endowed Fund in Support of Assistant Scientists at the Woods Hole Oceanographic Institution.2020-12-0

“Chapter 13: Sea Level Change” in Climate Change 2013: The Physical Science Basis: Contribution of Working Group I to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change

This chapter considers changes in global mean sea level, regional sea level, sea level extremes, and waves. Confidence in projections of global mean sea level rise has increased since the Fourth Assessment Report (AR4) because of the improved physical understanding of the components of sea level, the improved agreement of process-based models with observations, and the inclusion of ice-sheet dynamical changes

Asymmetric Response of Land Storage to ENSO Phase and Duration

Emergence of global mean sea level (GMSL) from a 'hiatus' following a persistent La Ni&ntilde;a highlights the need to understand the causes of interannual variability in GMSL. Several studies link interannual variability in GMSL to anomalous transport of water mass between land and ocean-and subsequent changes in water storage in these reservoirs-primarily driven by El Ni&ntilde;o/Southern Oscillation (ENSO). Despite this, asymmetries in teleconnections between ENSO mode and land water storage have received less attention. We use historical simulations of natural climate variability to characterize asymmetries in the hydrological response to ENSO based on phase and duration. Findings indicate pronounced phase-specific and duration-specific asymmetries covering up to 93 and 50 million km2 land area, respectively. The asymmetries are seasonally dependent, and based on the mean regional climate are capable of influencing inherently bounded storage by pushing the storage-precipitation relationship towards nonlinearity. The nonlinearities are more pronounced in dry regions in the dry season, wet regions in the wet season, and during Year 2 of persistent ENSO events, limiting the magnitude of associated anomalies under persistent ENSO influence. The findings have implications for a range of stakeholders, including sea level researchers and water managers.</p

An Evaluation of Contaminated Complete Feed as a Vehicle for Porcine Epidemic Diarrhea Virus Infection of Naïve Pigs Following Consumption Via Natural Feeding Behavior: Proof of Concept

Background: Since its initial detection in May 2013, porcine epidemic diarrhea virus (PEDV) has spread rapidly throughout the US swine industry. Initially, contaminated feed was proposed as a risk factor for PEDV; however, data were not available to support this theory. Here we provide proof of concept of this risk by describing a novel means for recovering PEDV-contaminated complete feed material from commercial swine sites and conducting an in vivo experiment to prove its infectivity. Results: For on-farm detection of PEDV RNA in feed, paint rollers were used to collect material from at-risk feed bins from 3 clinically affected breeding herds. This material was tested by PCR and determined to be positive for PEDV-RNA (Ct = 19.50-22.20 range). To test infectivity, this material was pooled (Ct = 20.65) and a Treatment group of 3-week old PEDV-naïve piglets were allowed to consume it via natural feeding behavior. For the purpose of a Positive control, piglets were allowed to ingest feed spiked with stock PEDV (Ct = 18.23) while the negative control group received PEDV-free feed. Clinical signs of PEDV infection (vomiting and diarrhea) and viral shedding were observed in both the Positive control and Treatment group’ post-consumption with virus and microscopic lesions detected in intestinal samples No evidence of infection was observed in the Negative controls.Conclusions: These data provide proof of concept that contaminated complete feed can serve as a vehicle for PEDV infection of naïve pigs using natural feeding behavior